Fusarium graminearum(sexual stage:Gibberella zeae)is the causative agent of Fusarium Head Blight(FHB),which is one of the most destructive plant disease of cereals,accounting for high grain yield losses,especially for...Fusarium graminearum(sexual stage:Gibberella zeae)is the causative agent of Fusarium Head Blight(FHB),which is one of the most destructive plant disease of cereals,accounting for high grain yield losses,especially for wheat and maize.Like other fungal pathogens,several extracellular enzymes secreted by G.zeae are known to be involved in host infection.Among these secreted lipases,G.zeae lipase(GZEL),which is encoded by the FGL1 gene,was demonstrated to be crucial to G.zeae pathogenicity.However,the precise mechanism of GZEL remains unclear due to a lack of detailed structural information.In this study,we report the crystal structure of GZEL at the atomic level.The structure of GZEL displays distinct structural differences compared to reported homologues and indicates a unique“double lock”enzymatic mechanism.To gain insight into substrate/inhibitor recognition,we proposed a model of GZEL in complex with substrate and the lipase inhibitor ebelactone B(based on the reported structures of GZEL homologues),which defines possible substrate binding sites within the catalytic cleft and suggests an“anti sn-l”binding mode.These results pave the way to elucidating the mechanism of GZEL and thus provide clues for the design of anti-FHB inhibitors.展开更多
2009年在河南省西峡县、唐河县采集得到玉米穗腐病样品,样品经病原分离和单孢子纯化后,得到3个菌株Gz1、Gz2与Gz3(GenBank登记号分别为:HM769949、HM769950与HQ110051).在致病性测定的基础上进行了rDNA-ITS序列分析,其结果表明3个菌株...2009年在河南省西峡县、唐河县采集得到玉米穗腐病样品,样品经病原分离和单孢子纯化后,得到3个菌株Gz1、Gz2与Gz3(GenBank登记号分别为:HM769949、HM769950与HQ110051).在致病性测定的基础上进行了rDNA-ITS序列分析,其结果表明3个菌株均与玉蜀黍赤霉菌(Gibberella zeae)有99%的同源性,用软件MEGA version 4.0构建基于r DNA-ITS序列系统发育树,3个菌株均与玉蜀黍赤霉菌在100%boostrap水平相聚于同一群;菌株Gz1、Gz2与Gz3的菌落形态和分生孢子特征均与文献描述的玉蜀黍赤霉菌相符.为此实验选取了Gz1作为代表性菌株对玉蜀黍赤霉菌的部分生物学特性做了研究.展开更多
基金supported by the National Programs for High Technology Research and Development Program of China(863 Program)(Grant No.2009ZX10004-304)the National Natural Science Foundation of China(NSFC)(Grant No.30870486)the National Major Project(Grant Nos.2009ZX09311-00,2006AA02A322).
文摘Fusarium graminearum(sexual stage:Gibberella zeae)is the causative agent of Fusarium Head Blight(FHB),which is one of the most destructive plant disease of cereals,accounting for high grain yield losses,especially for wheat and maize.Like other fungal pathogens,several extracellular enzymes secreted by G.zeae are known to be involved in host infection.Among these secreted lipases,G.zeae lipase(GZEL),which is encoded by the FGL1 gene,was demonstrated to be crucial to G.zeae pathogenicity.However,the precise mechanism of GZEL remains unclear due to a lack of detailed structural information.In this study,we report the crystal structure of GZEL at the atomic level.The structure of GZEL displays distinct structural differences compared to reported homologues and indicates a unique“double lock”enzymatic mechanism.To gain insight into substrate/inhibitor recognition,we proposed a model of GZEL in complex with substrate and the lipase inhibitor ebelactone B(based on the reported structures of GZEL homologues),which defines possible substrate binding sites within the catalytic cleft and suggests an“anti sn-l”binding mode.These results pave the way to elucidating the mechanism of GZEL and thus provide clues for the design of anti-FHB inhibitors.
文摘2009年在河南省西峡县、唐河县采集得到玉米穗腐病样品,样品经病原分离和单孢子纯化后,得到3个菌株Gz1、Gz2与Gz3(GenBank登记号分别为:HM769949、HM769950与HQ110051).在致病性测定的基础上进行了rDNA-ITS序列分析,其结果表明3个菌株均与玉蜀黍赤霉菌(Gibberella zeae)有99%的同源性,用软件MEGA version 4.0构建基于r DNA-ITS序列系统发育树,3个菌株均与玉蜀黍赤霉菌在100%boostrap水平相聚于同一群;菌株Gz1、Gz2与Gz3的菌落形态和分生孢子特征均与文献描述的玉蜀黍赤霉菌相符.为此实验选取了Gz1作为代表性菌株对玉蜀黍赤霉菌的部分生物学特性做了研究.
