On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cult...On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.展开更多
为进一步研究γ-醇溶蛋白1基因调控小麦面粉品质的分子机制,以郑麦158(低蛋白质含量、高面团强度)为研究材料,克隆小麦γ-醇溶蛋白1基因,构建诱饵载体pGBKT7-γ-醇溶蛋白1,利用酵母双杂交技术从郑麦158的cDNA文库中筛选γ-醇溶蛋白1的...为进一步研究γ-醇溶蛋白1基因调控小麦面粉品质的分子机制,以郑麦158(低蛋白质含量、高面团强度)为研究材料,克隆小麦γ-醇溶蛋白1基因,构建诱饵载体pGBKT7-γ-醇溶蛋白1,利用酵母双杂交技术从郑麦158的cDNA文库中筛选γ-醇溶蛋白1的候选互作蛋白,并通过回转验证试验进一步验证其互作关系。结果表明,成功克隆了γ-醇溶蛋白1基因,其cDNA全长为1 020 bp。利用酵母双杂交技术共筛选到117个蓝色单克隆菌落,经菌液PCR检测、测序以及NCBI的BLAST比对分析,共获得10个与γ-醇溶蛋白1互作的候选蛋白,它们分别是富含半胱氨酸和跨膜结构域蛋白1(XM_044546254.1)、α-淀粉酶抑制剂(XM_044509330.1)、特异性内切-1,3:1,4-β-D-葡聚糖酶2(XM_044550285.1、XM_044594213.1)、蔗糖果糖基转移酶(XM_044554537.1)、支链淀粉酶1(XM_044577151.1)和半胱氨酸蛋白酶B-like(XM_044577994.1),还有与植物生殖生长尤其是果实或者籽粒生长有关的细胞数目调控因子CNR8-like(XM_044483337.1、XM_044491902.1)、CNR2-like(XM_044468782.1)以及参与蛋白质修饰的泛素结构域蛋白DSK2b-like(Dominant suppressor of KAR 2b like,XM_044546840.1)和CIP73(CCa MK-interacting protein of 73 ku,XM_044554473.1)。候选互作蛋白的回转验证试验结果表明,富含半胱氨酸和跨膜结构域蛋白1、α-淀粉酶抑制剂、特异性内切-1,3:1,4-β-D-葡聚糖酶2和半胱氨酸蛋白酶B-like等4个蛋白质可能与γ-醇溶蛋白1存在互作关系。推测γ-醇溶蛋白1可能与这些蛋白质相互作用参与小麦种子中贮藏蛋白(如半胱氨酸)和淀粉的合成与降解等过程。展开更多
文摘On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.
文摘为进一步研究γ-醇溶蛋白1基因调控小麦面粉品质的分子机制,以郑麦158(低蛋白质含量、高面团强度)为研究材料,克隆小麦γ-醇溶蛋白1基因,构建诱饵载体pGBKT7-γ-醇溶蛋白1,利用酵母双杂交技术从郑麦158的cDNA文库中筛选γ-醇溶蛋白1的候选互作蛋白,并通过回转验证试验进一步验证其互作关系。结果表明,成功克隆了γ-醇溶蛋白1基因,其cDNA全长为1 020 bp。利用酵母双杂交技术共筛选到117个蓝色单克隆菌落,经菌液PCR检测、测序以及NCBI的BLAST比对分析,共获得10个与γ-醇溶蛋白1互作的候选蛋白,它们分别是富含半胱氨酸和跨膜结构域蛋白1(XM_044546254.1)、α-淀粉酶抑制剂(XM_044509330.1)、特异性内切-1,3:1,4-β-D-葡聚糖酶2(XM_044550285.1、XM_044594213.1)、蔗糖果糖基转移酶(XM_044554537.1)、支链淀粉酶1(XM_044577151.1)和半胱氨酸蛋白酶B-like(XM_044577994.1),还有与植物生殖生长尤其是果实或者籽粒生长有关的细胞数目调控因子CNR8-like(XM_044483337.1、XM_044491902.1)、CNR2-like(XM_044468782.1)以及参与蛋白质修饰的泛素结构域蛋白DSK2b-like(Dominant suppressor of KAR 2b like,XM_044546840.1)和CIP73(CCa MK-interacting protein of 73 ku,XM_044554473.1)。候选互作蛋白的回转验证试验结果表明,富含半胱氨酸和跨膜结构域蛋白1、α-淀粉酶抑制剂、特异性内切-1,3:1,4-β-D-葡聚糖酶2和半胱氨酸蛋白酶B-like等4个蛋白质可能与γ-醇溶蛋白1存在互作关系。推测γ-醇溶蛋白1可能与这些蛋白质相互作用参与小麦种子中贮藏蛋白(如半胱氨酸)和淀粉的合成与降解等过程。
