Objective:This review is aimed at explaining the psychological problems related to capillary blood glucose(CBG)testing and insulin injection,as well as recommending essential strategies to solve the fear thereof.Metho...Objective:This review is aimed at explaining the psychological problems related to capillary blood glucose(CBG)testing and insulin injection,as well as recommending essential strategies to solve the fear thereof.Methods:Databases,including PubMed,Cumulative Index of Nursing and Allied Health Literature(CINAHL),Scopus,and Google Scholar,were searched to extract the relevant articles.Initially,the terms used to retrieve related studies were"fear of blood glucose monitoring","anxiety capillary blood glucose testing and insulin injection","psychological problems on blood glucose monitoring and insulin injection","diabetes management",and"diabetes mellitus".Results:Results showed that the psychological problems related to CBG testing and insulin injection were associated with the stress and depression experienced during diabetes self-monitoring of blood glucose.This psychological issue has its impacts such as nonadherence to medication as well as a lack of self-discipline in terms of CBG testing and insulin injection.Inadequate information,inappropriate perception,and pain/discomfort during pricking of fingers were the main reasons for the psychological issues in CBG testing and self-injection of insulin.Conclusions:The expected benefits of this review include the explanation of the issues related to the psychological problems in CBG testing and insulin injection among type 2 diabetes mellitus(T2DM)patients.This review article also provides the recommendations on providing counseling and empowering the patients on CBG monitoring and insulin injection.Moreover,family members should provide psychological support to reduce fear,anxiety,and distress arising from CBG testing and insulin injection.展开更多
BACKGROUND： In studies concerning cell injury induced by cerebral ischemia-reperfusion, current experiments have primarily focused on altered protein levels. In addition, the apoptotic proteins Bax and Bcl-2 have bee...BACKGROUND： In studies concerning cell injury induced by cerebral ischemia-reperfusion, current experiments have primarily focused on altered protein levels. In addition, the apoptotic proteins Bax and Bcl-2 have been thoroughly studied with regard to initiating neuronal apoptosis. OBJECTIVE： To establish an in vitro model of oxygen-glucose deprivation and reintroduction in the rat hippocampus to simulate cerebral ischemia-reperfusion injury; to observe c-Jun N-terminal kinase 3 （JNK3） mRNA expression in hippocampal neurons following Astragalus injection; and thus to determine changes in the signaling and downstream pathways of neuronal apoptosis at the cellular and molecular level. DESIGN, TIME AND SETTING： A randomized, controlled, cellular and molecular experiment was performed at the Department of Central Laboratory, Chengde Medical College from February to June 2008. MATERIALS： Astragalus injection, the main ingredient of astragaloside, was purchased from Chengdu Di＇ao Jiuhong Pharmaceutical Manufactory, China. JNK3 mRNA probe and in situ hybridization kit were purchased from Tianjin Haoyang Biological Technology, China, and JNK3 RT-PCR primers were designed by Shanghai Bio-engineering, China. METHODS： Primary cultures of hippocampal neurons derived from Sprague Dawley rats, aged 1 2 days, were established. After 8 days, the hippocampal neurons were assigned to the following interventions： model group, Astragalus group, and vehicle control group, cells were subjected to oxygen-glucose reintroduction after oxygen-glucose deprivation for 30 minutes in sugar-free Earle＇s solution and a hypoxia device, which contained high-purity nitrogen. The normal control group was subjected to primary culture techniques and was not treated using above-mentioned interventions. In addition, the Astragalus and vehicle control groups were treated with Astragalus injection （0.5 g/L raw drug） or sterile, deionized water at 2 hours prior to oxygen-glucose deprivation, respectively. MAIN OUTCOME MEASURES： JNK3 mRNA expression was measured by in situ hybridization and RT-PCR at 0, 0.5, 2, 6, 24, 72, and 120 hours after oxygen-glucose reintroduction. RESULTS： Hippocampal neuronal morphology was normal in the normal control group. Hippocampal neurons exhibited apparent apoptosis-like pathological changes in the model, as well as the vehicle control, groups. The apoptosis-like pathological changes in the hippocampal neurons were less in the Astragalus group. Results from in situ hybridization and RT-PCR showed that JNK3 mRNA expression significantly increased in hippocampal neurons from model group, as well as the vehicle control group, compared with the normal control group （P 〈 0.05）. In addition, JNK3 mRNA expression significantly decreased in hippocampal neurons of the Astragalus group, compared with the model group and vehicle control group （P 〈 0.05）. CONCLUSION： Astragalus injection inhibited apoptosis-related JNK3 mRNA expression following oxygen-glucose deprivation and reintroduction, and accordingly played a role in inhibiting hippocampal neuronal apoptosis.展开更多
On the basis of oxidative decoloration of bromopyrogallol red （BPR） with H2O2, catalyzed by horseradish peroxidase（ HRP）, and the sequential injection renewable surface technique（ SI-RST）, a highly sensitive opt...On the basis of oxidative decoloration of bromopyrogallol red （BPR） with H2O2, catalyzed by horseradish peroxidase（ HRP）, and the sequential injection renewable surface technique（ SI-RST）, a highly sensitive optical-fiber sensor spectrophotometric method for the enzymatic determination of hydrogen peroxide was proposed. By coupling with a glucose oxidase（GOD）-catalyzed reaction, the method was used to determine glucose in human serum. The considerations in system and flow cell design, and factors that influence the determination performance are discussed. With 100μL of sample loaded and 0. 6 mg of bead trapped, the linear response range from 5.0 × 10^-8 to 5.2 × 10^-6 mol/L BPR with a detection limit（3σ） of 2. 5 ×10 ^-8 mol/L BPR, and a precision of 1.1% RSD（ n = 11 ） and a throughput of a 80 samples per hour can be achieved. Under the conditions of a 8. 7 × 10^ -6 mol/L BPR substrate, 0. 04 unit/mL HRP, 600 s reaction time and a reaction temperature of 37℃, the linear response range for H2O2 was from 5.0 × 10^-8 to 7.0 × 10^-6 mol/L with a detection limit（3σ） of 1.0 × 10^-8 mol/L and a precision of 3.7% RSD （ n = 11 ）. The linear response range by coupling with a GOD-catalyzed reaction was from 1.0 × 10^-7 to 1.0 × 10^-5 mol/L. The method was directly applied to determine glucose in human serum. Glucose contents obtained by the proposed procedure were compared with those obtained by using the phenol-4-AAP method, the error was found to be less than 3%.展开更多
Shuxuetong injection composed of leech(Hirudo nipponica Whitman) and earthworm(Pheretima aspergillum) has been used for the clinical treatment of acute stroke for many years in China. However, the precise neuroprotect...Shuxuetong injection composed of leech(Hirudo nipponica Whitman) and earthworm(Pheretima aspergillum) has been used for the clinical treatment of acute stroke for many years in China. However, the precise neuroprotective mechanism of Shuxuetong injection remains poorly understood. Here, cerebral microvascular endothelial cells(bEnd.3) were incubated in glucose-free Dulbecco's modified Eagle's medium containing 95% N_2/5% CO_2 for 6 hours, followed by high-glucose medium containing 95% O_2 and 5% CO_2 for 18 hours to establish an oxygen-glucose deprivation/reperfusion model. This in vitro cell model was administered Shuxuetong injection at 1/32, 1/64, and 1/128 concentrations(diluted 32-, 64-, and 128-times). Cell Counting Kit-8 assay was used to evaluate cell viability. A fluorescence method was used to measure lactate dehydrogenase, and a fluorescence microplate reader used to detect intracellular reactive oxygen species. A fluorescent probe was also used to measure mitochondrial superoxide production. A cell resistance meter was used to measure transepithelial resistance and examine integrity of monolayer cells. The fluorescein isothiocyanate-dextran test was performed to examine blood-brain barrier permeability. Real-time reverse transcription polymerase chain reaction was performed to analyze mRNA expression levels of tumor necrosis factor alpha, interleukin-1β, interleukin-6, and inducible nitric oxide synthase. Western blot assay was performed to analyze expression of caspase-3, intercellular adhesion molecule 1, vascular cell adhesion molecule 1, occludin, vascular endothelial growth factor, cleaved caspase-3, B-cell lymphoma 2, phosphorylated extracellular signal-regulated protein kinase, extracellular signal-regulated protein kinase, nuclear factor-κB p65, I kappa B alpha, phosphorylated I kappa B alpha, I kappa B kinase, phosphorylated I kappa B kinase, claudin-5, and zonula occludens-1. Our results show that Shuxuetong injection increases bEnd.3 cell viability and B-cell lymphoma 2 expression, reduces cleaved caspase-3 expression, inhibits production of reactive oxygen species and mitochondrial superoxide, suppresses expression of tumor necrosis factor alpha, interleukin-1β, interleukin-6, inducible nitric oxide synthase mRNA, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1, markedly increases transepithelial resistance, decreases blood-brain barrier permeability, upregulates claudin-5, occludin, and zonula occludens-1 expression, reduces nuclear factor-κB p65 and vascular endothelial growth factor expression, and reduces I kappa B alpha, extracellular signal-regulated protein kinase 1/2, and I kappa B kinase phosphorylation levels. Overall, these findings suggest that Shuxuetong injection has protective effects on brain microvascular endothelial cells after oxygen-glucose deprivation/reperfusion. Moreover, its protective effect is associated with reduction of mitochondrial superoxide production, inhibition of the inflammatory response, and inhibition of vascular endothelial growth factor, extracellular signal-regulated protein kinase 1/2, and the nuclear factor-κB p65 signaling pathway.展开更多
Impulsive injections of glucose and insulin analogues are very important strategies for the control of diabetes mellitus. We mainly imitate diabetes patients take insulin before eating, and eating approximately as a p...Impulsive injections of glucose and insulin analogues are very important strategies for the control of diabetes mellitus. We mainly imitate diabetes patients take insulin before eating, and eating approximately as a pulse blood glucose injection, as a result, a new mathematical model with impulsive injections of both glucose and insulin at different fixed times is formulated in this paper. Using the discrete dynamical system determined by the stroboscopic map, we show that the existence and uniqueness of a positive globally asymptotically stable periodic solution for type I diabetes. By impulsive comparison theorem, we obtain the glucose concentration level of the system is uniformly bounded above and below for type Ⅱ diabetes. Numerical analysis verifies our theoretical results.展开更多
Objective To examine the hyperglycemic effects of periocular dexamethasone injection in type 2 diabetic patients after vitreoretinal surgery （VRS）. Methods This was a retrospective non-randomized controlled trial. T...Objective To examine the hyperglycemic effects of periocular dexamethasone injection in type 2 diabetic patients after vitreoretinal surgery （VRS）. Methods This was a retrospective non-randomized controlled trial. Twenty consecutive hospitalized patients with type 2 diabetes and ocular inflammatory reaction after VRS were enrolled in this study. Ten patients received 2.5 mg dexamethasone and 10 patients received 5 mg dexamethasone. Fourteen consecutive type 2 diabetic patients without ocular inflammatory reaction after VRS were used as control group. We measured fasting blood glucose （FBG） and at 2 h after each meal （post prandial glucose, PBG; 09：00, 13：00, and 19：00 h） after periocular dexamethasone injection. Differences among three groups were determined by q tests. Results The PBG levels in both dexamethasone-treated groups started to increase within 5 h after injection （i.e., PBG at 13：00 h）, and were significantly increased at 29：00 h after injection （P〈0.05）. BG levels were almost 2-fold higher than at baseline and compared with the control group. The BG values declined gradually by 24 h to 48 h after injection. There were no differences in BG levels between the two dexamethasone-treated groups （P〉0.05）, except for PBG at 19：00 h on day 2 after injection （P〈0.05）. Conclusion Periocular dexamethasone injection can cause transient hyperglycemia in diabetic patients after VRS. BG monitoring should be performed following such injection.展开更多
Objective：To investigate the effects of Salvia Miltiorrhiza Liguspyragine Hydrochloride and Glucose Injection（参芎葡萄糖注射液,SLGI） on the expression of platelet membrane receptors proteinase-activated receptor-1...Objective：To investigate the effects of Salvia Miltiorrhiza Liguspyragine Hydrochloride and Glucose Injection（参芎葡萄糖注射液,SLGI） on the expression of platelet membrane receptors proteinase-activated receptor-1（PAR1） and proteinase-activated receptor-4（PAR4） in end-stage renal disease（ESRD） patients on chronic haemodialysis（HD）.Methods：Eighty-six ESRD patients on HD（treated group） were treated with SLGI,7 days as one therapeutic course,for two successive courses.The previous therapies were unchanged.Flow cytometry was used to assess the expression of platelet PAR1 and PAR4 in the patients,and turbidity method was used to determine the platelet maximum aggregation rate（MAR）.Meanwhile,renal function was measured.The final data were compared with those before treatment and with those in the normal control group（54 healthy subjects）.Results：Compared with the normal control group,the expressions of PAR1 and PAR4 and platelet MAR in ESRD patients on HD was significantly higher before treatment（P=0.001,P=0.006, and P=0.008）;after treatment with SLGI,the above indices in patients were remarkably decreased（P=0.036 and P=0.046）,except PAR4（P=0.067）,but still higher than those in the normal control group,however,it was not statistically significant.Conclusions：（1） The overexpression of PAR1 and PAR4 might lead to increased platelet aggregation and this could be one of the reasons for the thrombotic events in ESRD patients on HD.（2） SLGI was able to down-regulate the expression of PAR1 in ESRD patients on HD,improve platelet function,and regulate platelet activation.展开更多
目的:探究度拉糖肽注射液配合低碳水化合物早餐饮食疗法对老年糖尿病患者糖脂代谢紊乱的影响。方法:采用随机数字表法将84例2021年1月—2023年1月来单县中心医院内分泌科就诊的老年糖尿病患者分为对照组(42例)和试验组(42例),两组均予...目的:探究度拉糖肽注射液配合低碳水化合物早餐饮食疗法对老年糖尿病患者糖脂代谢紊乱的影响。方法:采用随机数字表法将84例2021年1月—2023年1月来单县中心医院内分泌科就诊的老年糖尿病患者分为对照组(42例)和试验组(42例),两组均予以常规药物治疗,在此基础上,对照组予以低碳水化合物早餐饮食疗法治疗,试验组则在对照组治疗基础上加用度拉糖肽注射液治疗,疗程均为3个月,对比两组治疗前后糖代谢水平、血脂指标水平、体重指数(BMI)、胰岛素水平。结果:经治疗后,两组空腹血糖(FPG)、餐后2 h血糖(2 h PG)及糖化血红蛋白(HbA1c)均较于治疗前明显下降,且试验组均低于对照组(P<0.05);试验组低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、甘油三酯(TG)均比对照组低(P<0.05),而高密度脂蛋白胆固醇(HDL-C)比对照组高(P<0.05);试验组BMI、空腹胰岛素(FINS)、餐后2 h胰岛素均低于对照组(P<0.05)。结论:采用度拉糖肽注射液配合低碳水化合物早餐饮食疗法对老年糖尿病治疗具有较好的效果,能够有效改善糖脂代谢异常和胰岛素功能,降低血糖与体质量。展开更多
基金the Layanan Beasiswa dan Pendanaan Riset Indonesia(LPDP)Scholarship scheme for supporting this study。
文摘Objective:This review is aimed at explaining the psychological problems related to capillary blood glucose(CBG)testing and insulin injection,as well as recommending essential strategies to solve the fear thereof.Methods:Databases,including PubMed,Cumulative Index of Nursing and Allied Health Literature(CINAHL),Scopus,and Google Scholar,were searched to extract the relevant articles.Initially,the terms used to retrieve related studies were"fear of blood glucose monitoring","anxiety capillary blood glucose testing and insulin injection","psychological problems on blood glucose monitoring and insulin injection","diabetes management",and"diabetes mellitus".Results:Results showed that the psychological problems related to CBG testing and insulin injection were associated with the stress and depression experienced during diabetes self-monitoring of blood glucose.This psychological issue has its impacts such as nonadherence to medication as well as a lack of self-discipline in terms of CBG testing and insulin injection.Inadequate information,inappropriate perception,and pain/discomfort during pricking of fingers were the main reasons for the psychological issues in CBG testing and self-injection of insulin.Conclusions:The expected benefits of this review include the explanation of the issues related to the psychological problems in CBG testing and insulin injection among type 2 diabetes mellitus(T2DM)patients.This review article also provides the recommendations on providing counseling and empowering the patients on CBG monitoring and insulin injection.Moreover,family members should provide psychological support to reduce fear,anxiety,and distress arising from CBG testing and insulin injection.
基金the Natural Science Foundation of Hebei Province,No.C2006000865
文摘BACKGROUND： In studies concerning cell injury induced by cerebral ischemia-reperfusion, current experiments have primarily focused on altered protein levels. In addition, the apoptotic proteins Bax and Bcl-2 have been thoroughly studied with regard to initiating neuronal apoptosis. OBJECTIVE： To establish an in vitro model of oxygen-glucose deprivation and reintroduction in the rat hippocampus to simulate cerebral ischemia-reperfusion injury; to observe c-Jun N-terminal kinase 3 （JNK3） mRNA expression in hippocampal neurons following Astragalus injection; and thus to determine changes in the signaling and downstream pathways of neuronal apoptosis at the cellular and molecular level. DESIGN, TIME AND SETTING： A randomized, controlled, cellular and molecular experiment was performed at the Department of Central Laboratory, Chengde Medical College from February to June 2008. MATERIALS： Astragalus injection, the main ingredient of astragaloside, was purchased from Chengdu Di＇ao Jiuhong Pharmaceutical Manufactory, China. JNK3 mRNA probe and in situ hybridization kit were purchased from Tianjin Haoyang Biological Technology, China, and JNK3 RT-PCR primers were designed by Shanghai Bio-engineering, China. METHODS： Primary cultures of hippocampal neurons derived from Sprague Dawley rats, aged 1 2 days, were established. After 8 days, the hippocampal neurons were assigned to the following interventions： model group, Astragalus group, and vehicle control group, cells were subjected to oxygen-glucose reintroduction after oxygen-glucose deprivation for 30 minutes in sugar-free Earle＇s solution and a hypoxia device, which contained high-purity nitrogen. The normal control group was subjected to primary culture techniques and was not treated using above-mentioned interventions. In addition, the Astragalus and vehicle control groups were treated with Astragalus injection （0.5 g/L raw drug） or sterile, deionized water at 2 hours prior to oxygen-glucose deprivation, respectively. MAIN OUTCOME MEASURES： JNK3 mRNA expression was measured by in situ hybridization and RT-PCR at 0, 0.5, 2, 6, 24, 72, and 120 hours after oxygen-glucose reintroduction. RESULTS： Hippocampal neuronal morphology was normal in the normal control group. Hippocampal neurons exhibited apparent apoptosis-like pathological changes in the model, as well as the vehicle control, groups. The apoptosis-like pathological changes in the hippocampal neurons were less in the Astragalus group. Results from in situ hybridization and RT-PCR showed that JNK3 mRNA expression significantly increased in hippocampal neurons from model group, as well as the vehicle control group, compared with the normal control group （P 〈 0.05）. In addition, JNK3 mRNA expression significantly decreased in hippocampal neurons of the Astragalus group, compared with the model group and vehicle control group （P 〈 0.05）. CONCLUSION： Astragalus injection inhibited apoptosis-related JNK3 mRNA expression following oxygen-glucose deprivation and reintroduction, and accordingly played a role in inhibiting hippocampal neuronal apoptosis.
文摘On the basis of oxidative decoloration of bromopyrogallol red （BPR） with H2O2, catalyzed by horseradish peroxidase（ HRP）, and the sequential injection renewable surface technique（ SI-RST）, a highly sensitive optical-fiber sensor spectrophotometric method for the enzymatic determination of hydrogen peroxide was proposed. By coupling with a glucose oxidase（GOD）-catalyzed reaction, the method was used to determine glucose in human serum. The considerations in system and flow cell design, and factors that influence the determination performance are discussed. With 100μL of sample loaded and 0. 6 mg of bead trapped, the linear response range from 5.0 × 10^-8 to 5.2 × 10^-6 mol/L BPR with a detection limit（3σ） of 2. 5 ×10 ^-8 mol/L BPR, and a precision of 1.1% RSD（ n = 11 ） and a throughput of a 80 samples per hour can be achieved. Under the conditions of a 8. 7 × 10^ -6 mol/L BPR substrate, 0. 04 unit/mL HRP, 600 s reaction time and a reaction temperature of 37℃, the linear response range for H2O2 was from 5.0 × 10^-8 to 7.0 × 10^-6 mol/L with a detection limit（3σ） of 1.0 × 10^-8 mol/L and a precision of 3.7% RSD （ n = 11 ）. The linear response range by coupling with a GOD-catalyzed reaction was from 1.0 × 10^-7 to 1.0 × 10^-5 mol/L. The method was directly applied to determine glucose in human serum. Glucose contents obtained by the proposed procedure were compared with those obtained by using the phenol-4-AAP method, the error was found to be less than 3%.
基金supported in part by the National Natural Science Foundation of China,No.81573644(to LMH),81573733(to SWX)the Tianjin 131 Innovative Team Project,China(to HW)+5 种基金the National Major Science and Technology Project of China,No.2012ZX09101201-004(to SWX)the Science and Technology Plan Project of Tianjin of China,No.16PTSYJC00120(to LMH)the Applied Foundation and Frontier Technology Research Program of Tianjin of China(General Project),No.14JCYBJC28900(to SXW)the National International Science and Technology Cooperation Project of China,No.2015DFA30430(to HW)the Key Program of the Natural Science Foundation of Tianjin of China,No.16ICZDJC36300(to HW)the Scientific Research and Technology Development Plan Project of Guangxi Zhuang Autonomous Region of China,No.14125008-2-5(to SXW)
文摘Shuxuetong injection composed of leech(Hirudo nipponica Whitman) and earthworm(Pheretima aspergillum) has been used for the clinical treatment of acute stroke for many years in China. However, the precise neuroprotective mechanism of Shuxuetong injection remains poorly understood. Here, cerebral microvascular endothelial cells(bEnd.3) were incubated in glucose-free Dulbecco's modified Eagle's medium containing 95% N_2/5% CO_2 for 6 hours, followed by high-glucose medium containing 95% O_2 and 5% CO_2 for 18 hours to establish an oxygen-glucose deprivation/reperfusion model. This in vitro cell model was administered Shuxuetong injection at 1/32, 1/64, and 1/128 concentrations(diluted 32-, 64-, and 128-times). Cell Counting Kit-8 assay was used to evaluate cell viability. A fluorescence method was used to measure lactate dehydrogenase, and a fluorescence microplate reader used to detect intracellular reactive oxygen species. A fluorescent probe was also used to measure mitochondrial superoxide production. A cell resistance meter was used to measure transepithelial resistance and examine integrity of monolayer cells. The fluorescein isothiocyanate-dextran test was performed to examine blood-brain barrier permeability. Real-time reverse transcription polymerase chain reaction was performed to analyze mRNA expression levels of tumor necrosis factor alpha, interleukin-1β, interleukin-6, and inducible nitric oxide synthase. Western blot assay was performed to analyze expression of caspase-3, intercellular adhesion molecule 1, vascular cell adhesion molecule 1, occludin, vascular endothelial growth factor, cleaved caspase-3, B-cell lymphoma 2, phosphorylated extracellular signal-regulated protein kinase, extracellular signal-regulated protein kinase, nuclear factor-κB p65, I kappa B alpha, phosphorylated I kappa B alpha, I kappa B kinase, phosphorylated I kappa B kinase, claudin-5, and zonula occludens-1. Our results show that Shuxuetong injection increases bEnd.3 cell viability and B-cell lymphoma 2 expression, reduces cleaved caspase-3 expression, inhibits production of reactive oxygen species and mitochondrial superoxide, suppresses expression of tumor necrosis factor alpha, interleukin-1β, interleukin-6, inducible nitric oxide synthase mRNA, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1, markedly increases transepithelial resistance, decreases blood-brain barrier permeability, upregulates claudin-5, occludin, and zonula occludens-1 expression, reduces nuclear factor-κB p65 and vascular endothelial growth factor expression, and reduces I kappa B alpha, extracellular signal-regulated protein kinase 1/2, and I kappa B kinase phosphorylation levels. Overall, these findings suggest that Shuxuetong injection has protective effects on brain microvascular endothelial cells after oxygen-glucose deprivation/reperfusion. Moreover, its protective effect is associated with reduction of mitochondrial superoxide production, inhibition of the inflammatory response, and inhibition of vascular endothelial growth factor, extracellular signal-regulated protein kinase 1/2, and the nuclear factor-κB p65 signaling pathway.
基金Supported by the Universities Young Teachers Program of Henan Province(2014GGJS-093)Supported by the Program for Science and Technology Innovation Talents in Universities of Henan Province(17HASTIT011)
文摘Impulsive injections of glucose and insulin analogues are very important strategies for the control of diabetes mellitus. We mainly imitate diabetes patients take insulin before eating, and eating approximately as a pulse blood glucose injection, as a result, a new mathematical model with impulsive injections of both glucose and insulin at different fixed times is formulated in this paper. Using the discrete dynamical system determined by the stroboscopic map, we show that the existence and uniqueness of a positive globally asymptotically stable periodic solution for type I diabetes. By impulsive comparison theorem, we obtain the glucose concentration level of the system is uniformly bounded above and below for type Ⅱ diabetes. Numerical analysis verifies our theoretical results.
文摘Objective To examine the hyperglycemic effects of periocular dexamethasone injection in type 2 diabetic patients after vitreoretinal surgery （VRS）. Methods This was a retrospective non-randomized controlled trial. Twenty consecutive hospitalized patients with type 2 diabetes and ocular inflammatory reaction after VRS were enrolled in this study. Ten patients received 2.5 mg dexamethasone and 10 patients received 5 mg dexamethasone. Fourteen consecutive type 2 diabetic patients without ocular inflammatory reaction after VRS were used as control group. We measured fasting blood glucose （FBG） and at 2 h after each meal （post prandial glucose, PBG; 09：00, 13：00, and 19：00 h） after periocular dexamethasone injection. Differences among three groups were determined by q tests. Results The PBG levels in both dexamethasone-treated groups started to increase within 5 h after injection （i.e., PBG at 13：00 h）, and were significantly increased at 29：00 h after injection （P〈0.05）. BG levels were almost 2-fold higher than at baseline and compared with the control group. The BG values declined gradually by 24 h to 48 h after injection. There were no differences in BG levels between the two dexamethasone-treated groups （P〉0.05）, except for PBG at 19：00 h on day 2 after injection （P〈0.05）. Conclusion Periocular dexamethasone injection can cause transient hyperglycemia in diabetic patients after VRS. BG monitoring should be performed following such injection.
基金Supported by the National Natural Science Foundation of China (No.30572441)
文摘Objective：To investigate the effects of Salvia Miltiorrhiza Liguspyragine Hydrochloride and Glucose Injection（参芎葡萄糖注射液,SLGI） on the expression of platelet membrane receptors proteinase-activated receptor-1（PAR1） and proteinase-activated receptor-4（PAR4） in end-stage renal disease（ESRD） patients on chronic haemodialysis（HD）.Methods：Eighty-six ESRD patients on HD（treated group） were treated with SLGI,7 days as one therapeutic course,for two successive courses.The previous therapies were unchanged.Flow cytometry was used to assess the expression of platelet PAR1 and PAR4 in the patients,and turbidity method was used to determine the platelet maximum aggregation rate（MAR）.Meanwhile,renal function was measured.The final data were compared with those before treatment and with those in the normal control group（54 healthy subjects）.Results：Compared with the normal control group,the expressions of PAR1 and PAR4 and platelet MAR in ESRD patients on HD was significantly higher before treatment（P=0.001,P=0.006, and P=0.008）;after treatment with SLGI,the above indices in patients were remarkably decreased（P=0.036 and P=0.046）,except PAR4（P=0.067）,but still higher than those in the normal control group,however,it was not statistically significant.Conclusions：（1） The overexpression of PAR1 and PAR4 might lead to increased platelet aggregation and this could be one of the reasons for the thrombotic events in ESRD patients on HD.（2） SLGI was able to down-regulate the expression of PAR1 in ESRD patients on HD,improve platelet function,and regulate platelet activation.
文摘目的:探究度拉糖肽注射液配合低碳水化合物早餐饮食疗法对老年糖尿病患者糖脂代谢紊乱的影响。方法:采用随机数字表法将84例2021年1月—2023年1月来单县中心医院内分泌科就诊的老年糖尿病患者分为对照组(42例)和试验组(42例),两组均予以常规药物治疗,在此基础上,对照组予以低碳水化合物早餐饮食疗法治疗,试验组则在对照组治疗基础上加用度拉糖肽注射液治疗,疗程均为3个月,对比两组治疗前后糖代谢水平、血脂指标水平、体重指数(BMI)、胰岛素水平。结果:经治疗后,两组空腹血糖(FPG)、餐后2 h血糖(2 h PG)及糖化血红蛋白(HbA1c)均较于治疗前明显下降,且试验组均低于对照组(P<0.05);试验组低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、甘油三酯(TG)均比对照组低(P<0.05),而高密度脂蛋白胆固醇(HDL-C)比对照组高(P<0.05);试验组BMI、空腹胰岛素(FINS)、餐后2 h胰岛素均低于对照组(P<0.05)。结论:采用度拉糖肽注射液配合低碳水化合物早餐饮食疗法对老年糖尿病治疗具有较好的效果,能够有效改善糖脂代谢异常和胰岛素功能,降低血糖与体质量。
基金Supported by the Key Program of Excellent Youth Foundation of Higher Education in Anhui Province(2013SQRL071ZD)the National Natural Science Foundation for Young Scholars of Anhui Province(1508085QA04)
