Aim: We measured Golgi protein73 (GP73), alpha-fetoprotein (AFP), and vascular endothelial growth factor (VEGF) expression in primary hepatic cancer (PHC) and assessed their clinical significance. Methods: Forty-five ...Aim: We measured Golgi protein73 (GP73), alpha-fetoprotein (AFP), and vascular endothelial growth factor (VEGF) expression in primary hepatic cancer (PHC) and assessed their clinical significance. Methods: Forty-five PHC and tumor-adjacent specimens and 14 normal liver specimens were examined. GP73, AFP, and VEGF expression was measured via immunohistochemistry and a correlation of protein expression with clinical pathology of PHC was suggested. Results: GP73, AFP, and VEGF expression was significantly higher in PHC and tumor-adjacent tissue compared to tumor-adjacent tissue (0.143 ± 0.018 vs. 0.124 ± 0.027, 0.116 ± 0.026 vs. 0.098 ± 0.014, and 0.126 ± 0.027 vs. 0.092 ± 0.016, respectively;all p 0.088 ± 0.029, 0.098 ± 0.014 vs. 0.073 ± 0.011, and 0.092 ± 0.016 vs. 0.076 ± 0.018, respectively;all p < 0.05), respectively. GP73 expression was positively correlated with pathological grade and cirrhosis, but not with tumor size, nodules, clinical stage and serum AFP. VEGF expression was positively correlated with tumor size, nodules, portal vein tumor thrombus, and clinical stage, but not with the degree of tumor differentiation and serum AFP. Expression of GP73 and VEGF was greater than that of AFP in PHC (both p < 0.05). Conclusion: GP73 is highly expressed in PHC and may be a diagnostic marker. Combined detection of GP73, AFP, and VEGF is helpful for diagnosis of PHC.展开更多
目的比较血清高尔基体蛋白73(GP73)与甲胎蛋白(AFP)对肝细胞癌(HCC)的诊断价值。方法检索TheCochrane Central Register of Controlled Trials、PubMed、EMBase、Web of Science、Nature、Science、Springer link、中国期刊全文数据库...目的比较血清高尔基体蛋白73(GP73)与甲胎蛋白(AFP)对肝细胞癌(HCC)的诊断价值。方法检索TheCochrane Central Register of Controlled Trials、PubMed、EMBase、Web of Science、Nature、Science、Springer link、中国期刊全文数据库、中国科技期刊数据库(维普)、万方数字化期刊全文数据库2012年10月以前公开发表的中、英文文献,收集关于血清GP73以及AFP诊断HCC的相关文献,按照纳入与排除标准筛选文献并进行信息统计、科学性评估。采用Meta-Disc 1.4软件进行数据分析,通过汇总后的敏感度、特异度、似然比及受试者工作特征(ROC)曲线综合评价血清GP73与AFP对HCC的诊断价值。结果共纳入18篇文献,样本量为5279例,其中HCC 2075例,非HCC的对照组3204例。通过随机效应模型计算得出血清GP73和AFP的汇总敏感度分别为0.78(0.76～0.79)和0.55(0.53～0.57),汇总特异度分别为0.84(0.83～0.86)和0.80(0.78～0.81),汇总阳性似然比分别为5.60(4.05～7.74)和2.92(2.04～4.17),汇总阴性似然比分别为0.28(0.24～0.32)和0.58(0.51～0.67),ROC曲线下面积分别为0.8849及0.6827,Z检验显示其差异具有统计学意义(P<0.01)。结论血清GP73对HCC有较高的诊断价值,可作为替代血清AFP的重要参考指标。展开更多
文摘Aim: We measured Golgi protein73 (GP73), alpha-fetoprotein (AFP), and vascular endothelial growth factor (VEGF) expression in primary hepatic cancer (PHC) and assessed their clinical significance. Methods: Forty-five PHC and tumor-adjacent specimens and 14 normal liver specimens were examined. GP73, AFP, and VEGF expression was measured via immunohistochemistry and a correlation of protein expression with clinical pathology of PHC was suggested. Results: GP73, AFP, and VEGF expression was significantly higher in PHC and tumor-adjacent tissue compared to tumor-adjacent tissue (0.143 ± 0.018 vs. 0.124 ± 0.027, 0.116 ± 0.026 vs. 0.098 ± 0.014, and 0.126 ± 0.027 vs. 0.092 ± 0.016, respectively;all p 0.088 ± 0.029, 0.098 ± 0.014 vs. 0.073 ± 0.011, and 0.092 ± 0.016 vs. 0.076 ± 0.018, respectively;all p < 0.05), respectively. GP73 expression was positively correlated with pathological grade and cirrhosis, but not with tumor size, nodules, clinical stage and serum AFP. VEGF expression was positively correlated with tumor size, nodules, portal vein tumor thrombus, and clinical stage, but not with the degree of tumor differentiation and serum AFP. Expression of GP73 and VEGF was greater than that of AFP in PHC (both p < 0.05). Conclusion: GP73 is highly expressed in PHC and may be a diagnostic marker. Combined detection of GP73, AFP, and VEGF is helpful for diagnosis of PHC.
文摘目的比较血清高尔基体蛋白73(GP73)与甲胎蛋白(AFP)对肝细胞癌(HCC)的诊断价值。方法检索TheCochrane Central Register of Controlled Trials、PubMed、EMBase、Web of Science、Nature、Science、Springer link、中国期刊全文数据库、中国科技期刊数据库(维普)、万方数字化期刊全文数据库2012年10月以前公开发表的中、英文文献,收集关于血清GP73以及AFP诊断HCC的相关文献,按照纳入与排除标准筛选文献并进行信息统计、科学性评估。采用Meta-Disc 1.4软件进行数据分析,通过汇总后的敏感度、特异度、似然比及受试者工作特征(ROC)曲线综合评价血清GP73与AFP对HCC的诊断价值。结果共纳入18篇文献,样本量为5279例,其中HCC 2075例,非HCC的对照组3204例。通过随机效应模型计算得出血清GP73和AFP的汇总敏感度分别为0.78(0.76～0.79)和0.55(0.53～0.57),汇总特异度分别为0.84(0.83～0.86)和0.80(0.78～0.81),汇总阳性似然比分别为5.60(4.05～7.74)和2.92(2.04～4.17),汇总阴性似然比分别为0.28(0.24～0.32)和0.58(0.51～0.67),ROC曲线下面积分别为0.8849及0.6827,Z检验显示其差异具有统计学意义(P<0.01)。结论血清GP73对HCC有较高的诊断价值,可作为替代血清AFP的重要参考指标。
