As a traditional herbal medicine,the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects.It is essential to develop an effective method for the determination ...As a traditional herbal medicine,the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects.It is essential to develop an effective method for the determination of the major alkaloids in U.rhynchophylla.In this research,a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids(corynoxeine,isocorynoxeine,isorhynchophylline,rhynchophylline)in U.rhynchophylla.Isorhynchophylline was selected as the internal reference substance,the content of which was determined by the traditional external standard method.Relative correction factors(RCF)between isorhynchophylline and the other three alkaloids were calculated respectively.The results showed that the QAMS method had good robustness under different HPLC instruments.Nineteen batches of U.rhynchophylla were tested.No significant difference was observed between the results by QAMS and EMS(Correlation coefficient>0.99,p>0.05).The QAMS method could be employed as a rapid,effective technique for the quality control of U.rhynchophylla.展开更多
Uncarialines A-E(1-5),five undescribed monoterpene indole alkaloids,together with five known analogues were obtained from the stems of Uncaria rhynchophylla.Alkaloids 1-3 were unique 3,4-seco-tricyclic alkaloids with ...Uncarialines A-E(1-5),five undescribed monoterpene indole alkaloids,together with five known analogues were obtained from the stems of Uncaria rhynchophylla.Alkaloids 1-3 were unique 3,4-seco-tricyclic alkaloids with a 6/5/10 ring system,while 4 and 5 possessed a rare rearranged scaffold originated from corynantheine-type alkaloids with C-2/C-7 oxidation.Their structures were characterized by a comprehensive analysis of MS,NMR,and ECD.Their effects on blood clotting times of human plasma were evaluated and alkaloid 5 had a slight prolongation effect on both thrombin time and activated partial thromboplastin time(p<0.001).展开更多
[Object] The research aimed to find out seed germination conditions and regulations.[Method] Seeds were soaked by clear water and 4 chemical agents of different concentrations(NaHCO3,KHCO3,K2CO3,NaClO)then conducted g...[Object] The research aimed to find out seed germination conditions and regulations.[Method] Seeds were soaked by clear water and 4 chemical agents of different concentrations(NaHCO3,KHCO3,K2CO3,NaClO)then conducted germination test in illumination box.[Result] Seed germination number and absolute germination rate(above 80%)in water treatment group was obviously higher than other treatments.[Conclusion] No dormancy phenomenon in mature embryo and no inhibition in seed coat,so conventional moist treatment can induct seed germination.展开更多
文摘As a traditional herbal medicine,the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects.It is essential to develop an effective method for the determination of the major alkaloids in U.rhynchophylla.In this research,a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids(corynoxeine,isocorynoxeine,isorhynchophylline,rhynchophylline)in U.rhynchophylla.Isorhynchophylline was selected as the internal reference substance,the content of which was determined by the traditional external standard method.Relative correction factors(RCF)between isorhynchophylline and the other three alkaloids were calculated respectively.The results showed that the QAMS method had good robustness under different HPLC instruments.Nineteen batches of U.rhynchophylla were tested.No significant difference was observed between the results by QAMS and EMS(Correlation coefficient>0.99,p>0.05).The QAMS method could be employed as a rapid,effective technique for the quality control of U.rhynchophylla.
基金Yunnan Applied Basic Research Projects(No.202301AS070057)National Key R&D Program of China(No.2022YFF1100301)Yunnan Revitalization Talents Support Plan-Young Talent Project(to Y.Zhang).
文摘Uncarialines A-E(1-5),five undescribed monoterpene indole alkaloids,together with five known analogues were obtained from the stems of Uncaria rhynchophylla.Alkaloids 1-3 were unique 3,4-seco-tricyclic alkaloids with a 6/5/10 ring system,while 4 and 5 possessed a rare rearranged scaffold originated from corynantheine-type alkaloids with C-2/C-7 oxidation.Their structures were characterized by a comprehensive analysis of MS,NMR,and ECD.Their effects on blood clotting times of human plasma were evaluated and alkaloid 5 had a slight prolongation effect on both thrombin time and activated partial thromboplastin time(p<0.001).
基金Supported by Demonstration Project of Cropping Structure Adjustment Comprehensive Technique"Research and Demonstration on Molecular Marker and High-yield Adjustment Technology of Uncaria,the Genuine Me-dicinal Material in Guizhou"(Project of Agriculture and Planting in Guizhou Agricultural Department[2005]08)~~
文摘[Object] The research aimed to find out seed germination conditions and regulations.[Method] Seeds were soaked by clear water and 4 chemical agents of different concentrations(NaHCO3,KHCO3,K2CO3,NaClO)then conducted germination test in illumination box.[Result] Seed germination number and absolute germination rate(above 80%)in water treatment group was obviously higher than other treatments.[Conclusion] No dormancy phenomenon in mature embryo and no inhibition in seed coat,so conventional moist treatment can induct seed germination.
