A thermo-insensitive pale green leaf mutant （pgl2） was isolated from T-DNA inserted transgenic lines of rice （Oryza sativa L. subsp, japonica cv. Nipponbare）. Genetic analysis indicated that the phenotype was caus...A thermo-insensitive pale green leaf mutant （pgl2） was isolated from T-DNA inserted transgenic lines of rice （Oryza sativa L. subsp, japonica cv. Nipponbare）. Genetic analysis indicated that the phenotype was caused by a recessive mutation in a single nuclear-encoded gene. To map the PGL2gene, an F2 population was constructed by crossing the mutant with Longtefu （Oryza sativa L. subsp, indica）. The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8. To finely map the gene, 14 new InDel markers were developed around the marker, and PGL2 was further mapped to a 2.37 Mb centromeric region. Analysis on chlorophyll contents of leaves showed that there was no obvious difference between the mutant and the wild type in total chlorophyll （Chl） content, while the ratio of Chl a / Chl b in the mutant was only about 1, which was distinctly lower than that in the wild type, suggesting that the PGL2 gene was related to the conversion between Chl a and Chl b. Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres.展开更多
P/TGMS (photo-thermo sensitive genie male sterility) lines with pale-green leaf color have been developed in japonica rice. The marker trait is used as an assistant selection in the production of the two-lines system ...P/TGMS (photo-thermo sensitive genie male sterility) lines with pale-green leaf color have been developed in japonica rice. The marker trait is used as an assistant selection in the production of the two-lines system hybrid rice for the improvement of F, seed purity. A joint inheritance study of both leaf color and male sterility is presented for P/TGMS line with pale-green leaf color. The segregation ratios for leaf color in the F2 populations of the three crosses showed 13 : 3 and 15 : 1 at early and late sowing stages (April 26 and June 23) respectively, implying that the leaf color is controlled by two genes with fertility gene as dominant. Sterility level is higher in the early sowing stage than that in the late sowing. The inducement of male sterility is closely related to longer day-length and higher temperature at the developmental stages of young panicle. The genes to govern the leaf color and male fertility are inherited independently.展开更多
The tea green leafhopper, Empoasca vitis Gothe, is one of the most serious insect pests of tea plantations in China's Mainland. Over the past decades, this pest has been controlled mainly by spraying pesticides. I...The tea green leafhopper, Empoasca vitis Gothe, is one of the most serious insect pests of tea plantations in China's Mainland. Over the past decades, this pest has been controlled mainly by spraying pesticides. Insecticide applications not only have become less effective in controlling damage, but even more seriously, have caused high levels of toxic residues in teas, which ultimately threatens human health. Therefore, we should seek a safer biological control approach. In the present study, key components of tea shoot volatiles were identified and behaviorally tested as potential leafhopper attractants. The following 13 volatile compounds were identified from aeration samples of tea shoots using gas chromatography-mass spectrometry （GC-MS）： （E）-2-hexenal, （Z）-3-hexen-1- ol, （Z）-3-hexenyl acetate, 2-ethyl-1-hexanol, （E）-ocimene, linalool, nonanol, （Z）-butanoic acid, 3-hexenyl ester, decanal, tetradecane, β-caryophyllene, geraniol and hexadecane. In Y-tube olfactometer tests, the following individual compounds were identified： （E）-2- hexenal, （E）-ocimene, （Z）-3-hexenyl acetate and linalool, as well as two synthetic mixtures （called blend 1 and blend 2） elicited significant taxis, with blend 2 being the most attractive. Blend 1 included linalool, （Z）-3-hexen-l-ol and （E）-2-hexenal at a 1： 1：1 ratio, whereas blend 2 was a mixture of eight compounds at the same loading ratio： （E）-2-hexenal, （Z）- 3-hexen-l-ol, （Z）-3-hexenyl acetate, 2-penten-l-ol, （E）-2-pentenal, pentanol, hexanol and 1-penten-3-ol. In tea fields, the bud-green sticky board traps baited with blend 2, （E）-2- hexenal or hexane captured adults and nymphs of the leafhoppers, with blend 2 being the most attractive, foUowed by （E）-2-hexenal and hexane. Placing sticky traps baited with blend 2 or （E）-2-hexenal in the tea fields significantly reduced leathopper populations. Our results indicate that the bud-green sticky traps baited with tea shoot volatiles can provide a new tool for monitoring and managing the tea leafhopper.展开更多
"WITH a dyed darkish-blue apron" was myself portrait when I first entered literarycircles. My hometown Chumen in Yuhuan is asmall town by the sea, a land of fish and ricein the southern reaches of the Changj..."WITH a dyed darkish-blue apron" was myself portrait when I first entered literarycircles. My hometown Chumen in Yuhuan is asmall town by the sea, a land of fish and ricein the southern reaches of the ChangjiangRiver. The gong and drum for the Liberationsounded in my childhood. I was infatuated bybooks then; the wing of ideas often entered mydreams like beautiful butterflies.展开更多
[Objectives] Introducing and screening leaf vegetable varieties tolerant to heat and humidity has great significance to ensure balanced supply of vegetables in summer and autumn in Hainan Province. [Methods] On the ba...[Objectives] Introducing and screening leaf vegetable varieties tolerant to heat and humidity has great significance to ensure balanced supply of vegetables in summer and autumn in Hainan Province. [Methods] On the basis of the introduction of green-stem non-heading Chinese cabbage and dark-green-leaf non-heading Chinese cabbage varieties, the varieties tolerant to heat and humidity were identified by the investigation of agronomic traits and evaluation of heat injury index. [Results] The data analysis results showed that four green-stem non-heading Chinese cabbage varieties including Huaguan Qingcaigeng F_1, Zhongguan Qinggeng, Hanshen No. 1 Qinggengcai F_1 and Huaxiawang Piaocai have the characteristics of strong tolerance to heat and high yield, and four dark-green-leaf non-heading Chinese cabbage varieties including Baimeigui, Heijingang, Xiaxue Naibaicai and Siji Baicai have the characteristics of strong tolerance to heat and high yield. [Conclusions] These eight leaf vegetable varieties are suitable for popularization in summer and autumn in Hainan Province.展开更多
The current urban green space construction was guided by a two-dimensional index evaluation system, resulting in weak ecological benefits of green space. Green plot ratio (GPR), as a three-dimensional indicator, can c...The current urban green space construction was guided by a two-dimensional index evaluation system, resulting in weak ecological benefits of green space. Green plot ratio (GPR), as a three-dimensional indicator, can characterize the ecological benefits of green areas and the ability of green areas to participate in the operation and regulation of urban ecosystems. As an important component of urban green space, the GPR index was added to the two-dimensional index evaluation system to optimize the green space, which can promote the development of low-carbon, healthy and ecological green space. Based on the research of 22 residential districts in the central city of Chongqing, the Leaf area index (LAI) index of common native garden plants in Chongqing was formed to improve the accuracy of green capacity index measurement in Chongqing. The study also took the residential community of Sunrise City in Banan District of Chongqing as an example, and carried out the optimization design practice from four types of residential community green areas: green areas next to houses, road green areas, concentrated green areas, and green areas attached to public service facilities.展开更多
文摘A thermo-insensitive pale green leaf mutant （pgl2） was isolated from T-DNA inserted transgenic lines of rice （Oryza sativa L. subsp, japonica cv. Nipponbare）. Genetic analysis indicated that the phenotype was caused by a recessive mutation in a single nuclear-encoded gene. To map the PGL2gene, an F2 population was constructed by crossing the mutant with Longtefu （Oryza sativa L. subsp, indica）. The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8. To finely map the gene, 14 new InDel markers were developed around the marker, and PGL2 was further mapped to a 2.37 Mb centromeric region. Analysis on chlorophyll contents of leaves showed that there was no obvious difference between the mutant and the wild type in total chlorophyll （Chl） content, while the ratio of Chl a / Chl b in the mutant was only about 1, which was distinctly lower than that in the wild type, suggesting that the PGL2 gene was related to the conversion between Chl a and Chl b. Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres.
基金This research was supported by a grant from China Nationl“863"High Tchnology Proramn,a key grant(2003C22007)“8812”Program from Zhejiang Province,China.
文摘P/TGMS (photo-thermo sensitive genie male sterility) lines with pale-green leaf color have been developed in japonica rice. The marker trait is used as an assistant selection in the production of the two-lines system hybrid rice for the improvement of F, seed purity. A joint inheritance study of both leaf color and male sterility is presented for P/TGMS line with pale-green leaf color. The segregation ratios for leaf color in the F2 populations of the three crosses showed 13 : 3 and 15 : 1 at early and late sowing stages (April 26 and June 23) respectively, implying that the leaf color is controlled by two genes with fertility gene as dominant. Sterility level is higher in the early sowing stage than that in the late sowing. The inducement of male sterility is closely related to longer day-length and higher temperature at the developmental stages of young panicle. The genes to govern the leaf color and male fertility are inherited independently.
文摘The tea green leafhopper, Empoasca vitis Gothe, is one of the most serious insect pests of tea plantations in China's Mainland. Over the past decades, this pest has been controlled mainly by spraying pesticides. Insecticide applications not only have become less effective in controlling damage, but even more seriously, have caused high levels of toxic residues in teas, which ultimately threatens human health. Therefore, we should seek a safer biological control approach. In the present study, key components of tea shoot volatiles were identified and behaviorally tested as potential leafhopper attractants. The following 13 volatile compounds were identified from aeration samples of tea shoots using gas chromatography-mass spectrometry （GC-MS）： （E）-2-hexenal, （Z）-3-hexen-1- ol, （Z）-3-hexenyl acetate, 2-ethyl-1-hexanol, （E）-ocimene, linalool, nonanol, （Z）-butanoic acid, 3-hexenyl ester, decanal, tetradecane, β-caryophyllene, geraniol and hexadecane. In Y-tube olfactometer tests, the following individual compounds were identified： （E）-2- hexenal, （E）-ocimene, （Z）-3-hexenyl acetate and linalool, as well as two synthetic mixtures （called blend 1 and blend 2） elicited significant taxis, with blend 2 being the most attractive. Blend 1 included linalool, （Z）-3-hexen-l-ol and （E）-2-hexenal at a 1： 1：1 ratio, whereas blend 2 was a mixture of eight compounds at the same loading ratio： （E）-2-hexenal, （Z）- 3-hexen-l-ol, （Z）-3-hexenyl acetate, 2-penten-l-ol, （E）-2-pentenal, pentanol, hexanol and 1-penten-3-ol. In tea fields, the bud-green sticky board traps baited with blend 2, （E）-2- hexenal or hexane captured adults and nymphs of the leafhoppers, with blend 2 being the most attractive, foUowed by （E）-2-hexenal and hexane. Placing sticky traps baited with blend 2 or （E）-2-hexenal in the tea fields significantly reduced leathopper populations. Our results indicate that the bud-green sticky traps baited with tea shoot volatiles can provide a new tool for monitoring and managing the tea leafhopper.
文摘"WITH a dyed darkish-blue apron" was myself portrait when I first entered literarycircles. My hometown Chumen in Yuhuan is asmall town by the sea, a land of fish and ricein the southern reaches of the ChangjiangRiver. The gong and drum for the Liberationsounded in my childhood. I was infatuated bybooks then; the wing of ideas often entered mydreams like beautiful butterflies.
基金Supported by Major Science and Technology Project of Hainan Province(ZDKJ2017001)
文摘[Objectives] Introducing and screening leaf vegetable varieties tolerant to heat and humidity has great significance to ensure balanced supply of vegetables in summer and autumn in Hainan Province. [Methods] On the basis of the introduction of green-stem non-heading Chinese cabbage and dark-green-leaf non-heading Chinese cabbage varieties, the varieties tolerant to heat and humidity were identified by the investigation of agronomic traits and evaluation of heat injury index. [Results] The data analysis results showed that four green-stem non-heading Chinese cabbage varieties including Huaguan Qingcaigeng F_1, Zhongguan Qinggeng, Hanshen No. 1 Qinggengcai F_1 and Huaxiawang Piaocai have the characteristics of strong tolerance to heat and high yield, and four dark-green-leaf non-heading Chinese cabbage varieties including Baimeigui, Heijingang, Xiaxue Naibaicai and Siji Baicai have the characteristics of strong tolerance to heat and high yield. [Conclusions] These eight leaf vegetable varieties are suitable for popularization in summer and autumn in Hainan Province.
文摘The current urban green space construction was guided by a two-dimensional index evaluation system, resulting in weak ecological benefits of green space. Green plot ratio (GPR), as a three-dimensional indicator, can characterize the ecological benefits of green areas and the ability of green areas to participate in the operation and regulation of urban ecosystems. As an important component of urban green space, the GPR index was added to the two-dimensional index evaluation system to optimize the green space, which can promote the development of low-carbon, healthy and ecological green space. Based on the research of 22 residential districts in the central city of Chongqing, the Leaf area index (LAI) index of common native garden plants in Chongqing was formed to improve the accuracy of green capacity index measurement in Chongqing. The study also took the residential community of Sunrise City in Banan District of Chongqing as an example, and carried out the optimization design practice from four types of residential community green areas: green areas next to houses, road green areas, concentrated green areas, and green areas attached to public service facilities.
