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Application of Rhododendron Species (Ericaceae) in Green System of Kunming City in Yunnan, China
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作者 ZHANG Jingli ZHAO Haizhou +1 位作者 WU Yawen WANG Yunyue 《Journal of Landscape Research》 2012年第10期63-67,共5页
Species, application frequencies, habitat features and combination modes of Rhododendron species in Kunming City were studied through random sampling in different subareas of Kunming City. The results show that Rhodod... Species, application frequencies, habitat features and combination modes of Rhododendron species in Kunming City were studied through random sampling in different subareas of Kunming City. The results show that Rhododendron species were widely applied in parks, residential areas and street-side green spaces. Diversified application patterns presented excellent landscape effects, but only a few species were used, for example, Rhododendron pulchrum Sweet., R. hybridum, R. simsii var. simsii, while R. delavayi Franch. and R. simsii var. mesembrinum Rehd. were occasionally found. Landscaping features of Kunming were characterized by domination of Rhododendron species, advantages and problems of Rhododendron species in landscaping of Kunming City were analyzed. It was proposed that introduction and domestication of wild Rhododendron species should be enhanced, rich germplasm resources of Rhododendron species should be fully used, and more indigenous Rhododendron species applied. 展开更多
关键词 RHODODENDRON SPECIES Kunming City green system SPECIES APPLICATION Plant furnishing pattern
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着眼提供有价值的整体解决方案 约翰迪尔Green System系列农具上市
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作者 汪芙蓉 《农机质量与监督》 2012年第4期44-44,共1页
在2012年约翰迪尔中国新产品发布会上,Green System系列农具正式亮相,现在,该系列产品已经在中国正式上市,这标志着约翰迪尔在中国的产品线已经扩展到农具系列产品,为中国用户提供有价值的整体解决方案。约翰迪尔服务中国36年,每年向广... 在2012年约翰迪尔中国新产品发布会上,Green System系列农具正式亮相,现在,该系列产品已经在中国正式上市,这标志着约翰迪尔在中国的产品线已经扩展到农具系列产品,为中国用户提供有价值的整体解决方案。约翰迪尔服务中国36年,每年向广大用户提供数以万计的产品,深受广大用户的欢迎。约翰迪尔的成功来自于深入理解客户的需要,不断向客户提供有价值的产品和服务, 展开更多
关键词 整体解决方案 约翰迪尔 green 价值 农具 上市 新产品发布会 中国用户
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提供有价值的整体解决方案——Green System系列农具上市
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作者 汪芙蓉 《农机科技推广》 2012年第4期62-62,共1页
在2012年约翰迪尔中国新产品发布会上,Green System系列农具正式亮相,现在,该系列产品已经在中国正式上市,这标志着约翰迪尔在中国的产品线已经扩展到农具系列产品,为中国用户提供有价值的整体解决方案。
关键词 整体解决方案 green 农具 价值 上市 新产品发布会 中国用户 约翰迪尔
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提供有价值的整体解决方案——约翰迪尔Green System系列农具上市
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《农机市场》 2012年第4期60-60,共1页
在2012年约翰迪尔中国新产品发布会上,Green System系列农具正式亮相,现在,该系列产品已经在中国正式上市,这标志着约翰迪尔在中国的产品线已经扩展到农具系列产品,为中国用户提供有价值的整体解决方案。
关键词 整体解决方案 green 约翰迪尔 农具 价值 上市 新产品发布会 中国用户
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提供有价值的整体解决方案——Green System系列农具上市
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《农村科技》 2012年第5期73-73,共1页
2012年3月26日,在2012年约翰迪尔中国新产品发布会上,Green System系列农具正式亮相。现在,该系列产品已经在中国正式上市,这标志着约翰迪尔在中国的产品线已经扩展到农具系列产品,为中国用户提供有价值的整体解决方案。
关键词 整体解决方案 green 农具 价值 上市 新产品发布会 中国用户 约翰迪尔
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Coupling and coordination relationship of tourism inclusive green growth system:Evidence from Shandong Province
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作者 Tianjun Xu Gangmin Weng Wei Guo 《Chinese Journal of Population,Resources and Environment》 2024年第3期334-342,共9页
Sustainable growth is a prerequisite for high-quality development,and inclusive green growth is regarded as an important way to realize sustainable growth.This paper constructs a tourism inclusive green growth index(I... Sustainable growth is a prerequisite for high-quality development,and inclusive green growth is regarded as an important way to realize sustainable growth.This paper constructs a tourism inclusive green growth index(IGGI)system based on the 2018 Asian Development Bank IGGI.Using Shandong Province from 2017 to 2019,the entropy weight method is utilized to explore the degree of coupling and coordination among the subsystems of tourism inclusive green growth.The study shows that:(1)the tourism IGGI continues to grow;(2)in the tourism inclusive green growth system,the social equity subsystem shows a trend of steady improvement;and(3)in the system,there is a slight discordance between the economic growth subsystem and the environmental sustainability subsystem.The study aims to provide the scientific basis for the high-quality development of tourism and theoretical support for the field of tourism and environmental sustainability. 展开更多
关键词 Tourism inclusive green growth system Coupled coordination Entropy law Tourism quality development
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How Marie Sklodowska-Curie Doctoral Networks Can Support European Refractory Ecosystem in Context of The European Green Deal
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作者 Marc HUGER Glyn DERRICK +4 位作者 Mossaab MOUIYA Andrea SALERNO Kwasi Addo BOATENG Hrikeshava RANGANATHAN Amit Kumar GOPE 《China's Refractories》 CAS 2024年第2期1-9,共9页
The movement of the Iron&Steelmaking(I&S)industry towards Net-Zero emissions and digitalized processes through disruptive,breakthrough technologies will be achieved through the use of Hydrogen.The biggest chal... The movement of the Iron&Steelmaking(I&S)industry towards Net-Zero emissions and digitalized processes through disruptive,breakthrough technologies will be achieved through the use of Hydrogen.The biggest challenge for the refractory industry is to continue to meet the performance expectations while,at the same time,moving to a more sustainable production direction.The complexity and urgency of these technological changes,highlighted by the European Green Deal,requires ambitious,international,interdisciplinary and intersectoral projects,bringing together institutes from across the global value chain,to carry out cutting edge research.The European Union,through its flagship doctoral training program,MSCA,has,and continues to support research and development as well as the promotion of the refractory industry in Europe.An introduction to two MSCA projects and some of the results achieved are highlighted within this article. 展开更多
关键词 FIRE network Horizon 2020 Horizon Europe Marie Sklodowska-Curie Actions Doctoral networks European green Deal
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Comparative Analysis for the Development of a Green Building Rating System for Nigeria
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作者 Usman Aminu Umar 《Journal of Civil Engineering and Architecture》 2024年第7期340-347,共8页
The functionality of buildings depends on the climate they are subjected to,this means buildings constructed these days need to be built to perform efficiently within the current and future climate and with the aim of... The functionality of buildings depends on the climate they are subjected to,this means buildings constructed these days need to be built to perform efficiently within the current and future climate and with the aim of reducing the greenhouse emission induced on the environment.In order to lower CO_(2) emissions and assist in reinforcing the capability of cities to adapt to climate change whilst enhancing the quality of the built environment,it is vital to improve the environmental performance and energy efficiency in buildings.This study aims to identify and compare existing GBRS(green building rating system)with Nigerian climatic condition.Proposal criteria weighting was developed from the questionnaire supplying expert opinions.It began with a comparative analysis of GBRS.The analysis was conducted to find the similarities and differences between existing rating systems and come up with an appropriate rating system for Nigeria.These approaches included a fieldwork approach(pilot study,survey);questionnaire;and interviews,both structured and unstructured.Then,the information generated in this pre-test investigation formed the conversational guide and described the decision to determine the main factors that should be involved in the new assessment system for Nigeria. 展开更多
关键词 green building GBRS climate change NIGERIA
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Impact on Soil Organic C and Total Soil N from Cool- and Warm-Season Legumes Used in a Green Manure-Forage Cropping System
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作者 Clark B. Neely Francis M. Rouquette Jr. +3 位作者 Cristine L.S. Morgan Frank M. Hons William L. Rooney Gerald R. Smith 《Agricultural Sciences》 2024年第3期333-357,共25页
Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their... Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their seasonal biomass production can be managed to complement forage grasses. Our research objectives were to evaluate both warm- and cool-season annual forage legumes as green manure for biomass, N content, ability to enhance soil organic carbon (SOC) and soil N, and impact on post season forage grass crops. Nine warm-season forage legumes (WSL) were spring planted and incorporated as green manure in the fall. Forage rye (Secale cereale L.) was planted following the incorporation of WSL treatments. Eight cool-season forage legumes (CSL) were fall planted in previously fallow plots and incorporated as green manure in late spring. Sorghum-sudangrass (Sorghum bicolor x Sorghum bicolor var. sudanense) was planted over all treatments in early summer after forage rye harvest and incorporation of CSL treatments. Sorghum-sudangrass was harvested in June, August and September, and treatments were evaluated for dry matter and N concentration. Soil cores were taken from each plot, split into depths of 0 to 15, 15 to 30 and 30 to 60 cm, and soil C and N were measured using combustion analysis. Nylon mesh bags containing plant samples were buried at 15 cm and used to evaluate decomposition rate of above ground legume biomass, including change in C and N concentrations. Mungbean (Vigna radiata L. [Wilczek]) had the highest shoot biomass yield (6.24 t DM ha<sup>-1</sup>) and contributed the most total N (167 kg∙ha<sup>-1</sup>) and total C (3043 kg∙ha<sup>-1</sup>) of the WSL tested. Decomposition rate of WSL biomass was rapid in the first 10 weeks and very slow afterward. Winter pea (Pisum sativum L. spp. sativum), arrow leaf clover (Trifolium vesiculosum Savi.), and crimson clover (Trifolium incarnatum L.) were the most productive CSL in this trial. Austrian winter pea produced 8.41 t DM ha<sup>-1</sup> with a total N yield of 319 kg N ha<sup>-1</sup> and total C production of 3835 kg C ha<sup>-1</sup>. The WSL treatments had only small effects on rye forage yield and N concentration, possibly due to mineralization of N from a large SOC pool already in place. The CSL treatments also had only minimal effects on sorghum-sudangrass forage production. Winter pea, arrow leaf and crimson clover were productive cool season legumes and could be useful as green manure crops. Mungbean and cowpea (Vigna unguiculata [L.] Walp.) were highly productive warm season legumes but may include more production risk in green manure systems due to soil moisture competition. 展开更多
关键词 Annual Legumes Soil N Soil Organic C green Manure Deer Browse Forage Cropping systems
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System and Realization Path of Xi Jinping's Eco-poverty Alleviation Concept under the Vision of Green Development
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作者 Xieyu XIONG Jialin LIU Wanxuan WU 《Meteorological and Environmental Research》 2024年第3期23-31,共9页
Since the 18 th National Congress of the Communist Party of China,General Secretary Xi Jinping delivered a series of speeches on ecological environment and poverty governance,forming the Xi Jinping's eco-poverty a... Since the 18 th National Congress of the Communist Party of China,General Secretary Xi Jinping delivered a series of speeches on ecological environment and poverty governance,forming the Xi Jinping's eco-poverty alleviation ideology.In this paper,the Xi Jinping's eco-poverty alleviation ideology is taken as the research object.Using the methods of literature analysis and comparative analysis,the origin of eco-poverty alleviation theory is explored from the common prosperity theory and the theory of ecological capital,and the practice foundation is found out from practical cases.The relationship between the ecological damage and poverty is systematically analyzed,and the mechanism of ecological poverty is explored in detail.The basic connotation of Xi Jinping's eco-poverty alleviation is expounded,and it consists of three parts:guiding ideology layer,implementation layer,and guarantee layer.By perfecting and innovating the market mechanism,social management mechanism,performance evaluation mechanism,integration mechanism with other poverty alleviation methods,and ecological immigration mechanism of ecological poverty,the realization of Xi Jinping's eco-poverty alleviation concept could be guaranteed. 展开更多
关键词 green transformation Xi Jinping's eco-poverty alleviation Ecological capital
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Construction of Regional Ecological Capital Operation Mechanism System from the Perspective of Green Economy Development
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作者 Wanxuan WU Jialin LIU Xieyu XIONG 《Meteorological and Environmental Research》 2024年第4期44-48,共5页
Green development has gradually become the main theme of the current world economic development.Green transformation development and cracking the contradiction between ecology and economy need to build an effective ec... Green development has gradually become the main theme of the current world economic development.Green transformation development and cracking the contradiction between ecology and economy need to build an effective ecological capital operation system and mechanism to support,encourage the effective operation of regional ecological capital,promote the appreciation and preservation of ecological capital,and escort the operation.This paper constructs a four-in-one regional ecological capital operation mechanism system,in which the"accumulation mechanism"is the prerequisite,the"conversion mechanism"is the key link,the"compensation mechanism"is an important supplement,and the"incentive mechanism"is the safeguard measure,which aims to ensure the smooth progress of the construction of beautiful China in the new era and realize the socialist modern power. 展开更多
关键词 green economy Value realization of ecological products Ecological capital operation
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Research on the Index System of Old Neighborhood Renovation Based on Green Building Evaluation Standard
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作者 Yufei Que 《Journal of Architectural Research and Development》 2024年第3期140-146,共7页
With the advancement of the transformation,the contradiction between the residents’demand for a better living environment and the convenience of living in the settlements comes to the fore.Effective identification,or... With the advancement of the transformation,the contradiction between the residents’demand for a better living environment and the convenience of living in the settlements comes to the fore.Effective identification,organic integration,timely adoption,and correct decision-making for the transformation of old neighborhoods are pressing issues in the transformation of old neighborhoods.Therefore,this paper takes the green building evaluation standards of various countries as the research basis and support for the construction of the transformation strategy of old neighborhoods.Through the collection and comparative analysis of the indicators of green building evaluation standards,the index system of transformation is formed,and it also provides a certain foundation for the subsequent related research. 展开更多
关键词 Old neighborhoods green building evaluation standards RENOVATION CRITERIA
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水禽细小病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立与应用
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作者 汪宏才 商雨 +7 位作者 马瑶 曾哲 张蓉蓉 姚伦 罗玲 李丽 温国元 罗青平 《湖北农业科学》 2024年第6期218-222,共5页
为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.... 为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.77;除WPV出现S形扩增曲线外,新城疫病毒(NDV)、H9亚型禽流感病毒(H9 AIV)、鸭坦布苏病毒(DTMUV)、鸭肝炎病毒(DHAV)、鸭肠炎病毒(DEV)、鸭呼肠孤病毒(DRV)样品均未出现S形阳性扩增曲线;批内变异系数(CV)为0.15%~0.23%,批间变异系数为0.09%~0.28%。结果表明,SYBR Green Ⅰ荧光定量PCR检测方法重复性好、灵敏度高和特异性强。临床样品检测结果表明,SYBR Green Ⅰ荧光定量PCR与普通PCR的符合率达98.4%,灵敏度是普通PCR的1 000倍。SYBR Green Ⅰ荧光定量PCR检测方法不仅能定性检测WPV,还可以进行定量检测,可用于种鸭场、种鹅场的WPV净化检测,也可用于WPV临床大量样品的快速检测。 展开更多
关键词 水禽细小病毒 检测方法 SYBR green 荧光定量PCR
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盆底超声检查和Green分型诊断产妇产后早期盆底结构及压力性尿失禁
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作者 黄雪 周京晶 +3 位作者 李梦妮 白炜 容亚娟 邵越 《中国计划生育学杂志》 2024年第6期1444-1447,共4页
目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsa... 目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsalva动作状态下膀胱颈距耻骨联合下缘的距离(BSD)、膀胱尿道后角(PUA)、膀胱颈移动度(BND)和尿道旋转角(UR),对比Green分型;采用受试者工作特征(ROC)曲线分析相关参数诊断SUI价值。结果:静息状态下,两组盆底超声参数无差异(P>0.05);SUI组Valsalva动作状态下的BSD、PUA、BND和UR值均大于非SUI组,Green分型Ι型占比(25.6%)低于非SUI组(59.8%)(均P<0.05)。ROC曲线分析,Green分型曲线下面积(AUC)0.579,对SUI诊断价值不佳(P=0.057),Valsalva动作状态下BSD(AUC=0.758)、PUA(AUC=0.743)、BND(AUC=0.769)和UR(AUC=0.705)对SUI诊断价值良好(均P<0.05)。结论:盆底超声检查和Green分型能有效评估经产妇早期盆底结构状况,前者可诊断SUI。 展开更多
关键词 压力性尿失禁 产后早期 盆底结构 超声检查 green分型 诊断
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约束Green函数与非线性地基梁模态分析 被引量:1
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作者 赵翔 王琦 +1 位作者 朱伟东 李映辉 《动力学与控制学报》 2024年第2期77-84,共8页
在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础... 在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础上的梁振动模型,研究了任意位置弹簧和非线性弹簧基础上的梁模态.通过Laplace变换和线性叠加原理,得到了一种约束Green函数,利用数值计算验证方案的有效性,并研究了各种重要物理参数的影响,发现弹簧位置向跨中移动时,模态对称性被打破,弹簧刚度增加,模态阶数改变. 展开更多
关键词 约束green函数 LAPLACE变换 非线性弹簧基础 模态分析 叠加原理
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Evolutionary game-based optimization of green certificate-carbon emission right-electricity joint market for thermal-wind-photovoltaic power system 被引量:1
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作者 Ran Wang Yanhe Li Bingtuan Gao 《Global Energy Interconnection》 EI CAS CSCD 2023年第1期92-102,共11页
With the increasing proportion of renewable energy in the power market,the demands on government financial subsidies are gradually increasing.Thus,a joint green certificate-carbon emission right-electricity multi-mark... With the increasing proportion of renewable energy in the power market,the demands on government financial subsidies are gradually increasing.Thus,a joint green certificate-carbon emission right-electricity multi-market trading process is proposed to study the market-based strategy for renewable energy.Considering the commodity characteristics of green certificates and carbon emission rights,the dynamic cost models of green certificates and carbon rights are constructed based on the Rubinstein game and ladder pricing models.Furthermore,considering the irrational bidding behavior of energy suppliers in the actual electricity market,an evolutionary game based multi-market bidding optimization model is presented.Subsequently,it is solved using a composite differential evolutionary algorithm.Finally,the case study results reveal that the proposed model can increase profits and the consumption rate of renewable energy and reduce carbon emission. 展开更多
关键词 Electricity market Carbon emission right green certificate Evolutionary game
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Global energy transition revolution and the connotation and pathway of the green and intelligent energy system 被引量:1
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作者 ZOU Caineng MA Feng +10 位作者 PAN Songqi ZHAO Qun FU Guoyou ZHANG Guosheng YANG Yichao YU Hao LIANG Yingbo LIN Minjie WANG Ying XIONG Bo LIU Hanlin 《Petroleum Exploration and Development》 SCIE 2023年第3期722-740,共19页
The essence of energy system transition is the"energy revolution':The development of the"resource-dominated"energy system with fossil energy as the mainstay has promoted human progress,but it has al... The essence of energy system transition is the"energy revolution':The development of the"resource-dominated"energy system with fossil energy as the mainstay has promoted human progress,but it has also triggered energy crisis and ecological environment crisis,which is not compatible with the new demands of the new round of scientific and technological revolution,industrial transformation,and sustainable human development.It is in urgent need to research and develop a new-type energy system in the context of carbon neutrality.In the framework of"technique-dominated"new green and intelligent energy system with"three new"of new energy,new power and new energy storage as the mainstay,the"super energy basin"concepts with the Ordos Basin,Nw China as a representative will reshape the concept and model of future energy exploration and development.In view of the"six inequalities"in global energy and the resource conditions of"abundant coal,insufficient oil and gas and infinite new energy"in China,it is suggested to deeply boost"China energy revolution',sticking to the six principles of independent energy production,green energy supply,secure energy reserve,efficient energy consumption,intelligent energy management,economical energy cost;enhance"energy scientific and technological innovation"by implementing technique-dominated"four major science and technology innovation projects',namely,clean coal project,oil production stabilization and gas production increasing project,new energy acceleration project,and green-intelligent energy project;implement"energy transition"by accelerating the green-dominated"four-modernization development',namely,fossil energy cleaning,large-scale new energy,coordinated centralized energy distribution,intelligent multi-energy management,so as to promote the exchange of two 80%s"in China's energy structure and construct the new green and intelligent energy system. 展开更多
关键词 carbon neutrality new energy energy revolution primitive energy system ancient energy system neoteric energy system modern energy system new-type energy system green and intelligent energy system super energy basin Ordos Basin
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猪流行性腹泻病毒SYBR Green I实时荧光定量PCR检测方法的建立
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作者 董苏洁 孔宁 +9 位作者 秦文珍 翟焕杰 翟雪滢 杨心雨 童武 郑浩 于海 童光志 李有文 单同领 《中国动物传染病学报》 CAS 北大核心 2024年第2期174-180,共7页
为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。... 为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。 展开更多
关键词 猪流行性腹泻病毒 N基因 SYBR green I 荧光定量PCR
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Design and implementation of railway green performance basic data collection system 被引量:1
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作者 Xiangru Lyu Hui Li 《High-Speed Railway》 2023年第4期265-272,共8页
This study addressed the issues related to the collection and management of basic data for railway green performance. A railway green performance basic database has been constructed based on metadata and data exchange... This study addressed the issues related to the collection and management of basic data for railway green performance. A railway green performance basic database has been constructed based on metadata and data exchange schemas. A data classification system has been established from the perspectives of businesses, processes,and entities. A BIM(Building Information Modelling) model data extraction scheme is proposed based on field similarity matching and a document content extraction scheme is proposed based on image recognition. A railway green performance basic data collection system has been developed, achieving efficient collection and integrated management of railway green performance basic data. This system can provide data support for applications such as railway carbon emissions accounting, green cost-benefit analysis, and evaluation of green design solutions. 展开更多
关键词 RAILWAY green performance Data collection METADATA Data classifcation BIM Image recognition
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美洲鳗鲡腺瘤病毒(AEAdoV)普通PCR和SYBR Green I实时荧光定量PCR检测方法的建立及应用
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作者 孔文迪 陈曦 +1 位作者 杨金先 葛均青 《水产学报》 CAS CSCD 北大核心 2024年第4期358-365,共8页
为建立美洲鳗鲡腺瘤病毒(AEAdoV)的检测方法,根据AEAdoV福建株(AEAdoVFJ)的superfamily 3 helicases(S3H)序列,设计引物,建立了AEAdoV的普通PCR和qPCR检测方法;进一步评价检测方法的灵敏性、特异性及重复性,利用2种方法对美洲鳗鲡“出... 为建立美洲鳗鲡腺瘤病毒(AEAdoV)的检测方法,根据AEAdoV福建株(AEAdoVFJ)的superfamily 3 helicases(S3H)序列,设计引物,建立了AEAdoV的普通PCR和qPCR检测方法;进一步评价检测方法的灵敏性、特异性及重复性,利用2种方法对美洲鳗鲡“出血性烂鳃”病料进行了检测,并对美洲鳗鲡体内不同组织的病毒含量进行分析。结果显示,普通PCR扩增的目的片段长度约300 bp,利用其构建的qPCR质粒标准品,其拷贝数与qPCR阈值循环数(C_(t))线性关系良好,线性范围广,标准曲线相关系数(R2)达到0.999,扩增效率为105.067%。建立的普通PCR法和qPCR的最低检测AEAdoV拷贝数分别为100个和10个。2种方法均可特异性检测AEAdoV,而对蛙虹彩病毒(RGV)、鳗鲡疱疹病毒(AngHV)、鲤疱疹病毒(KHV)、对虾白斑综合征病毒(WSSV)、日本鳗鲡内皮细胞坏死病毒(JEAdoV)和花鳗鲡腺瘤病毒(MEAdoV)均无扩增反应。qPCR法的组内和组间变异系数均小于2%,表明其重复性良好。临床应用结果显示,35份美洲鳗鲡“出血性烂鳃”病料,采用普通PCR法的AEAdoV检出率为82.8%,而qPCR法的AEAdoV检出率为97%。对美洲鳗鲡不同组织的病毒含量分析结果显示,心脏、肝脏、鳃、鳍的AEAdoV相对含量较高,而黏液、皮肤和脾脏的病毒含量相对较低。研究表明,建立的AEAdoV的灵敏度高、特异性强的普通PCR和qPCR检测方法,证实AEAdoV与美洲鳗鲡“出血性烂鳃”病密切相关,且在感染鳗鲡主要组织中都存在。实验结果对于研究AEAdoV的致病性,开展其流行情况和病原学情况分析具有重要意义。 展开更多
关键词 美洲鳗鲡腺瘤病毒(AEAdoV) 普通PCR 荧光定量PCR SYBR green 检测方法
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