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Impact of Energy Efficiency and Financial Support on Green Upgrading in China’s Industrial Sector
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作者 Yingying Zhou Ning Yang +2 位作者 Panpan Meng Xiaoqian Jing Fengran Lu 《Proceedings of Business and Economic Studies》 2023年第6期24-29,共6页
The report from the 20th National Congress of China emphasizes the importance of focusing on the clean,low-carbon,and efficient use of energy,increasing financial support,and promoting green upgrading within the indus... The report from the 20th National Congress of China emphasizes the importance of focusing on the clean,low-carbon,and efficient use of energy,increasing financial support,and promoting green upgrading within the industrial sector.This paper,based on annual data,employs the entropy weight method to construct a comprehensive index reflecting the impact of green upgrading in industrial sectors.To delve deeper,it utilizes the DEA model to measure energy efficiency and its subdivision BCC model to break down energy efficiency into technical and scale efficiency.The financial support landscape is examined from the vantage points of both direct and indirect financing.Using a multivariate time series model,this paper thoroughly investigates the influence of energy efficiency and financial support on the green upgrading of the industrial sector.The findings reveal a significant positive impact of both energy efficiency and financial support on green upgrading in industrial industries.Notably,scale efficiency emerges as the primary driver of energy efficiency.Moreover,indirect financing proves to be more effective in promoting financial support than direct financing.The empirical results retain their robustness even after substituting explanatory variables.The study concludes by contextualizing the research findings within the current real-world scenario,offering practical insights,and proposing specific recommendations. 展开更多
关键词 Energy efficiency Financial support Industrial green upgrading DEA-BCC model
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Green up-conversion Luminescence in Nd^(3+)-Ho^(3+) Co-doped Oxyfluorotellurite Glasses
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作者 邱建备 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2007年第B12期809-812,共4页
Blue, green and red up-conversion luminescence at around 490, 545 and 650 nm, which result from the Ho^3+5F3→5I8, (5F4,5S2)→3. 5I8 and 5F5→ 5I8 transitions, respectively, were observed in Nd3+-Ho3. co-doped oxy... Blue, green and red up-conversion luminescence at around 490, 545 and 650 nm, which result from the Ho^3+5F3→5I8, (5F4,5S2)→3. 5I8 and 5F5→ 5I8 transitions, respectively, were observed in Nd3+-Ho3. co-doped oxyfiuorotellurite glasses under 800 nm excitation. Among these up-conversion luminescence, the green emission was extremely strong and the blue and red emission intensities were very weak. Selectively strong green up-conversion luminescence of these glasses indicate a high possibility for realizing a green upconversion laser. Up-conversion processes for the blue, green and red emissions are two-photon processes assisted by Nd3^+→Ho^3+ energy transfer. It is proposed that the up-conversion mechanism for the blue and green emissions is different from that for the red emission. The respective mechanisms are discussed. 展开更多
关键词 up-conversion luminescence oxyfiuorotellurite glass blue and greene emission
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Deep eutectic solvents:Green multi-task agents for sustainable super green hydrogen technologies
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作者 Raiyan Al-Farsi Maan Hayyan 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第5期357-382,共26页
While reliance on renewable energy resources has become a reality, there is still a need to deploy greener and more sustainable methods in order to achieve sustainable development goals. Indeed, green hydrogen is curr... While reliance on renewable energy resources has become a reality, there is still a need to deploy greener and more sustainable methods in order to achieve sustainable development goals. Indeed, green hydrogen is currently believed to be a reliable solution for global warming and the pollution challenges arising from fossil fuels, making it the resilient fuel of the future. However, the sustainability of green hydrogen technologies is yet to be achieved. In this context, generation of green hydrogen with the aid of deep eutectic solvents(DESs) as green mixtures has been demonstrated as a promising research area. This systematic review article covers green hydrogen generation through water splitting and biomass fermentation when DESs are utilized within the generation process. It also discusses the incorporation of DESs in fuel cell technologies. DESs can play a variety of roles such as solvent, electrolyte, or precursor;colloidal suspension and reaction medium;galvanic replacement, shape-controlling, decoration, or extractive agent;finally oxidant. These roles are relevant to several methods of green hydrogen generation, including electrocatalysis, photocatalysis, and fermentation. As such, it is of utmost importance to screen potential DES formulations and determine how they can function in and contribute throughout the green hydrogen mobility stages. The realization of super green hydrogen generation stands out as a pivotal milestone in our journey towards achieving a more sustainable form of development;DESs have great potential in making this milestone achievable. Overall, incorporating DESs in hydrogen generation constitutes a promising research area and offers potential scalability for green hydrogen production, storage,transport, and utilization. 展开更多
关键词 Watersplitting BIOHYDROGEN Super green hydrogen ELECTROCATALYSIS PHOTOCATALYSIS Fuel cell Power-to-X
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Artificial selection of the Green Revolution gene Semidwarf 1 is implicated in upland rice breeding
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作者 Shuliang Jiao Qinyan Li +6 位作者 Fan Zhang Yonghong Tao Yingzhen Yu Fan Yao Qingmao Li Fengyi Hu Liyu Huang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第3期769-780,共12页
Semidwarf breeding has boosted crop production and is a well-known outcome from the first Green Revolution. The Green Revolution gene Semidwarf 1(SD1), which modulates gibberellic acid(GA) biosynthesis, plays a princi... Semidwarf breeding has boosted crop production and is a well-known outcome from the first Green Revolution. The Green Revolution gene Semidwarf 1(SD1), which modulates gibberellic acid(GA) biosynthesis, plays a principal role in determining rice plant height. Mutations in SD1 reduce rice plant height and promote lodging resistance and fertilizer tolerance to increase grain production. The plant height mediated by SD1 also favors grain yield under certain conditions. However, it is not yet known whether the function of SD1 in upland rice promotes adaptation and grain production. In this study, the plant height and grain yield of irrigated and upland rice were comparatively analyzed under paddy and dryland conditions. In response to dryland environments, rice requires a reduction in plant height to cope with water deficits. Upland rice accessions had greater plant heights than their irrigated counterparts under both paddy and dryland conditions, and appropriately reducing plant height could improve adaptability to dryland environments and maintain high grain yield formation. Moreover, upland rice cultivars with thicker stem diameters had stronger lodging resistance, which addresses the lodging problem. Knockout of SD1 in the upland rice cultivar IRAT104 reduced the plant height and grain yield, demonstrating that the adjustment of plant height mediated by SD1 could increase grain production in dryland fields. In addition, an SD1 genetic diversity analysis verified that haplotype variation causes phenotypic variation in plant height. During the breeding history of rice, SD1 allelic mutations were selected from landraces to improve the grain yield of irrigated rice cultivars, and this selection was accompanied by a reduction in plant height. Thus, five known mutant alleles were analyzed to verify that functional SD1 is required for upland rice production. All these results suggest that SD1 might have undergone artificial positive selection in upland rice, which provides further insights concerning greater plant height in upland rice breeding. 展开更多
关键词 green Revolution Semidwarf 1 high-yield breeding upland rice plant height
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Legume green manure can intensify the function of chemical nitrogen fertilizer substitution via increasing nitrogen supply and uptake of wheat
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作者 Jingui Wei Zhilong Fan +5 位作者 Falong Hu Shoufa Mao Fang Yin Qiming Wang Qiang Chai Wen Yin 《The Crop Journal》 SCIE CSCD 2024年第4期1222-1232,共11页
Achieving the green development of agriculture requires the reduction of chemical nitrogen(N)fertilizer input.Previous studies have confirmed that returning green manure to the field is an effective measure to improve... Achieving the green development of agriculture requires the reduction of chemical nitrogen(N)fertilizer input.Previous studies have confirmed that returning green manure to the field is an effective measure to improve crop yields while substituting partial chemical N fertilizer.However,it remains unclear how to further intensify the substituting function of green manure and elucidate its underlying agronomic mechanism.In a split-plot field experiment in spring wheat,different green manures returned to the field under reduced chemical N supply was established in an oasis area since 2018,in order to investigate the effect of green manure and reduced N on grain yield,N uptake,N use efficiency(NUE),N nutrition index,soil organic matter,and soil N of wheat in 2020-2022.Our results showed that mixed sown common vetch and hairy vetch can substitute 40%of chemical N fertilizer without reducing grain yield or N accumulation.Noteworthily,mixed sown common vetch and hairy vetch under reduced N by 20%showed the highest N agronomy efficiency and recovery efficiency,which were 92.0%and 46.0%higher than fallow after wheat harvest and conventional N application rate,respectively.The increase in NUE of wheat was mainly attributed to mixed sown common vetch and hairy vetch,which increased N transportation quantity and transportation rate at pre-anthesis,enhanced N harvest index,optimized N nutrition index,and increased activities of nitrate reductase and glutamine synthetase of leaf,respectively.Meanwhile,mixed sown common vetch and hairy vetch under reduced N by 20%improved soil organic matter and N contents.Therefore,mixed sown common vetch and hairy vetch can substitute 40%of chemical N fertilizer while maintaining grain yield and N accumulation,and it combined with reduced chemical N by 20%or 40%improved NUE of wheat via enhancing N supply and uptake. 展开更多
关键词 Legume green manure Reduced N input WHEAT N uptake and supply Agronomic mechanism
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Coupling and coordination relationship of tourism inclusive green growth system:Evidence from Shandong Province
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作者 Tianjun Xu Gangmin Weng Wei Guo 《Chinese Journal of Population,Resources and Environment》 2024年第3期334-342,共9页
Sustainable growth is a prerequisite for high-quality development,and inclusive green growth is regarded as an important way to realize sustainable growth.This paper constructs a tourism inclusive green growth index(I... Sustainable growth is a prerequisite for high-quality development,and inclusive green growth is regarded as an important way to realize sustainable growth.This paper constructs a tourism inclusive green growth index(IGGI)system based on the 2018 Asian Development Bank IGGI.Using Shandong Province from 2017 to 2019,the entropy weight method is utilized to explore the degree of coupling and coordination among the subsystems of tourism inclusive green growth.The study shows that:(1)the tourism IGGI continues to grow;(2)in the tourism inclusive green growth system,the social equity subsystem shows a trend of steady improvement;and(3)in the system,there is a slight discordance between the economic growth subsystem and the environmental sustainability subsystem.The study aims to provide the scientific basis for the high-quality development of tourism and theoretical support for the field of tourism and environmental sustainability. 展开更多
关键词 Tourism inclusive green growth system Coupled coordination Entropy law Tourism quality development
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Synergistic effects of three traditional herbs green tea,mulberry leaf and corn silk on glucose uptake level of L6 myoblasts and the hypoglycemic mechanism
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作者 Jing-Na Zhou Ming-Yue Li +4 位作者 Ting-Ting Zhang Jing-Yang Lu Min Zhang Peng-Wei Zhuang Hai-Xia Chen 《Traditional Medicine Research》 2024年第7期1-12,共12页
Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear... Background:Green tea,mulberry leaf and corn silk are traditional herbs used in the prevention and treatment of diabetes in China for a long time,but their synergistic hypoglycemic effects and mechanisms remain unclear.Methods:The effective components of green tea,mulberry leaf and corn silk were extracted and enriched.Mixture design of experiments was used to study the influences of different combinations on the cell viability and glucose uptake level of L6 myoblasts,so as to determine the optimal synergistic hypoglycemic combination.The possible hypoglycemic mechanism of the optimal synergistic combination was explored by cytotoxicity assay,glucose uptake assay,and western blot.Results:Three polyphenol enrichment fractions of the herbs,30%ethanol elution fraction of green tea(GT),50%ethanol elution fraction of mulberry leaf(ML)and 60%ethanol elution fraction of corn silk(CS)were obtained.The antioxidant activities of GT-30%,ML-50%and CS-60%were superior to those of crude extracts,and showed strong potential inα-amylase andα-glucosidase inhibition activities.The optimal synergistic combination of crude extracts G7(crude extract of green tea:crude extract of mulberry leaf:crude extract of corn silk=1:5:3),polyphenol enrichment fractions R3(GT-30%:ML-50%:CS-60%=1:7:1)and monomers X2(epigallocatechin gallate:morusin:formononetin=3:1:2)were selected,respectively.G7,R3,and X2 showed promoting effects on the cell viability and glucose uptake of L6 myoblasts within the detected concentration range.In addition,G7,R3,and X2 could increase the expression levels of p-PI3K/PI3K and p-Akt/Akt in L6 myoblasts,and promote the translocation of Glut4,but G7 and R3 showed more significant effects.Conclusion:The synergistic hypoglycemic effects of green tea,mulberry leaf and corn silk had the characteristics of multiple-components and multiple-targets with p-PI3K/PI3K,p-Akt/Akt and the translocation of Glut4 signal pathways involved.The three traditional herbs might have the potential to be combined used for the prevention and treatment of diabetes based on the synergistic hypoglycemic effects. 展开更多
关键词 green tea mulberry leaf corn silk synergistic effect type 2 diabetes mellitus mechanism
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How Marie Sklodowska-Curie Doctoral Networks Can Support European Refractory Ecosystem in Context of The European Green Deal
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作者 Marc HUGER Glyn DERRICK +4 位作者 Mossaab MOUIYA Andrea SALERNO Kwasi Addo BOATENG Hrikeshava RANGANATHAN Amit Kumar GOPE 《China's Refractories》 CAS 2024年第2期1-9,共9页
The movement of the Iron&Steelmaking(I&S)industry towards Net-Zero emissions and digitalized processes through disruptive,breakthrough technologies will be achieved through the use of Hydrogen.The biggest chal... The movement of the Iron&Steelmaking(I&S)industry towards Net-Zero emissions and digitalized processes through disruptive,breakthrough technologies will be achieved through the use of Hydrogen.The biggest challenge for the refractory industry is to continue to meet the performance expectations while,at the same time,moving to a more sustainable production direction.The complexity and urgency of these technological changes,highlighted by the European Green Deal,requires ambitious,international,interdisciplinary and intersectoral projects,bringing together institutes from across the global value chain,to carry out cutting edge research.The European Union,through its flagship doctoral training program,MSCA,has,and continues to support research and development as well as the promotion of the refractory industry in Europe.An introduction to two MSCA projects and some of the results achieved are highlighted within this article. 展开更多
关键词 FIRE network Horizon 2020 Horizon Europe Marie Sklodowska-Curie Actions Doctoral networks European green Deal
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Research on the Impact of BYD Group’s Green Innovation on Corporate Financial Performance under the Background of“Dual Carbon”
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作者 Ziyue Liao 《Proceedings of Business and Economic Studies》 2024年第4期207-213,共7页
With the increasingly severe global climate change problem,the“dual carbon”goals(peak carbon emissions and carbon neutrality)have become a common focus of international attention.The report of the 20th National Cong... With the increasingly severe global climate change problem,the“dual carbon”goals(peak carbon emissions and carbon neutrality)have become a common focus of international attention.The report of the 20th National Congress of the Communist Party of China clearly emphasizes the need to accelerate the green transformation of development models,implement comprehensive strategies for frugal development,support the growth of green and low-carbon industries,and promote the concept of green consumption.At the same time,“Made in China 2025”also elaborates on the strategic concept of innovation-driven and green development centered,and strives for breakthroughs in key industries such as new energy vehicles.In such a macro environment,adopting green innovation measures by enterprises not only contributes to ecological protection but also has an undeniable impact on their economic performance and overall value.This article takes BYD Group as a case study to explore in detail the positive effects of green innovation on its economic performance.We first systematically organized and analyzed BYD’s specific practices in green innovation;Then,by examining three key financial indicators,BYD’s current financial situation was analyzed in depth;On this basis,combined with research data,the positive impact of green innovation on BYD’s financial performance was revealed;Finally,based on the analysis results,relevant suggestions are proposed to provide reference for the sustainable development of enterprises in the context of“dual carbon.” 展开更多
关键词 Dual carbon target BYD Group green innovation Corporate financial performance
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水禽细小病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立与应用
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作者 汪宏才 商雨 +7 位作者 马瑶 曾哲 张蓉蓉 姚伦 罗玲 李丽 温国元 罗青平 《湖北农业科学》 2024年第6期218-222,共5页
为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.... 为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.77;除WPV出现S形扩增曲线外,新城疫病毒(NDV)、H9亚型禽流感病毒(H9 AIV)、鸭坦布苏病毒(DTMUV)、鸭肝炎病毒(DHAV)、鸭肠炎病毒(DEV)、鸭呼肠孤病毒(DRV)样品均未出现S形阳性扩增曲线;批内变异系数(CV)为0.15%~0.23%,批间变异系数为0.09%~0.28%。结果表明,SYBR Green Ⅰ荧光定量PCR检测方法重复性好、灵敏度高和特异性强。临床样品检测结果表明,SYBR Green Ⅰ荧光定量PCR与普通PCR的符合率达98.4%,灵敏度是普通PCR的1 000倍。SYBR Green Ⅰ荧光定量PCR检测方法不仅能定性检测WPV,还可以进行定量检测,可用于种鸭场、种鹅场的WPV净化检测,也可用于WPV临床大量样品的快速检测。 展开更多
关键词 水禽细小病毒 检测方法 SYBR green 荧光定量PCR
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盆底超声检查和Green分型诊断产妇产后早期盆底结构及压力性尿失禁
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作者 黄雪 周京晶 +3 位作者 李梦妮 白炜 容亚娟 邵越 《中国计划生育学杂志》 2024年第6期1444-1447,共4页
目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsa... 目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsalva动作状态下膀胱颈距耻骨联合下缘的距离(BSD)、膀胱尿道后角(PUA)、膀胱颈移动度(BND)和尿道旋转角(UR),对比Green分型;采用受试者工作特征(ROC)曲线分析相关参数诊断SUI价值。结果:静息状态下,两组盆底超声参数无差异(P>0.05);SUI组Valsalva动作状态下的BSD、PUA、BND和UR值均大于非SUI组,Green分型Ι型占比(25.6%)低于非SUI组(59.8%)(均P<0.05)。ROC曲线分析,Green分型曲线下面积(AUC)0.579,对SUI诊断价值不佳(P=0.057),Valsalva动作状态下BSD(AUC=0.758)、PUA(AUC=0.743)、BND(AUC=0.769)和UR(AUC=0.705)对SUI诊断价值良好(均P<0.05)。结论:盆底超声检查和Green分型能有效评估经产妇早期盆底结构状况,前者可诊断SUI。 展开更多
关键词 压力性尿失禁 产后早期 盆底结构 超声检查 green分型 诊断
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约束Green函数与非线性地基梁模态分析 被引量:1
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作者 赵翔 王琦 +1 位作者 朱伟东 李映辉 《动力学与控制学报》 2024年第2期77-84,共8页
在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础... 在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础上的梁振动模型,研究了任意位置弹簧和非线性弹簧基础上的梁模态.通过Laplace变换和线性叠加原理,得到了一种约束Green函数,利用数值计算验证方案的有效性,并研究了各种重要物理参数的影响,发现弹簧位置向跨中移动时,模态对称性被打破,弹簧刚度增加,模态阶数改变. 展开更多
关键词 约束green函数 LAPLACE变换 非线性弹簧基础 模态分析 叠加原理
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猪流行性腹泻病毒SYBR Green I实时荧光定量PCR检测方法的建立
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作者 董苏洁 孔宁 +9 位作者 秦文珍 翟焕杰 翟雪滢 杨心雨 童武 郑浩 于海 童光志 李有文 单同领 《中国动物传染病学报》 CAS 北大核心 2024年第2期174-180,共7页
为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。... 为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。 展开更多
关键词 猪流行性腹泻病毒 N基因 SYBR green I 荧光定量PCR
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美洲鳗鲡腺瘤病毒(AEAdoV)普通PCR和SYBR Green I实时荧光定量PCR检测方法的建立及应用
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作者 孔文迪 陈曦 +1 位作者 杨金先 葛均青 《水产学报》 CAS CSCD 北大核心 2024年第4期358-365,共8页
为建立美洲鳗鲡腺瘤病毒(AEAdoV)的检测方法,根据AEAdoV福建株(AEAdoVFJ)的superfamily 3 helicases(S3H)序列,设计引物,建立了AEAdoV的普通PCR和qPCR检测方法;进一步评价检测方法的灵敏性、特异性及重复性,利用2种方法对美洲鳗鲡“出... 为建立美洲鳗鲡腺瘤病毒(AEAdoV)的检测方法,根据AEAdoV福建株(AEAdoVFJ)的superfamily 3 helicases(S3H)序列,设计引物,建立了AEAdoV的普通PCR和qPCR检测方法;进一步评价检测方法的灵敏性、特异性及重复性,利用2种方法对美洲鳗鲡“出血性烂鳃”病料进行了检测,并对美洲鳗鲡体内不同组织的病毒含量进行分析。结果显示,普通PCR扩增的目的片段长度约300 bp,利用其构建的qPCR质粒标准品,其拷贝数与qPCR阈值循环数(C_(t))线性关系良好,线性范围广,标准曲线相关系数(R2)达到0.999,扩增效率为105.067%。建立的普通PCR法和qPCR的最低检测AEAdoV拷贝数分别为100个和10个。2种方法均可特异性检测AEAdoV,而对蛙虹彩病毒(RGV)、鳗鲡疱疹病毒(AngHV)、鲤疱疹病毒(KHV)、对虾白斑综合征病毒(WSSV)、日本鳗鲡内皮细胞坏死病毒(JEAdoV)和花鳗鲡腺瘤病毒(MEAdoV)均无扩增反应。qPCR法的组内和组间变异系数均小于2%,表明其重复性良好。临床应用结果显示,35份美洲鳗鲡“出血性烂鳃”病料,采用普通PCR法的AEAdoV检出率为82.8%,而qPCR法的AEAdoV检出率为97%。对美洲鳗鲡不同组织的病毒含量分析结果显示,心脏、肝脏、鳃、鳍的AEAdoV相对含量较高,而黏液、皮肤和脾脏的病毒含量相对较低。研究表明,建立的AEAdoV的灵敏度高、特异性强的普通PCR和qPCR检测方法,证实AEAdoV与美洲鳗鲡“出血性烂鳃”病密切相关,且在感染鳗鲡主要组织中都存在。实验结果对于研究AEAdoV的致病性,开展其流行情况和病原学情况分析具有重要意义。 展开更多
关键词 美洲鳗鲡腺瘤病毒(AEAdoV) 普通PCR 荧光定量PCR SYBR green 检测方法
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香辛料SYBR GREENⅠ染料实时聚合酶链式反应检测方法的建立及应用
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作者 杨晴丽 王仁静 +6 位作者 张茹 孟宪卓 姚帮本 陈赵然 张旭 方建军 陈伟 《食品科学》 EI CAS CSCD 北大核心 2024年第12期269-275,共7页
目的:本研究致力于建立香辛料真实性成分的分子鉴定方法,为香辛料的掺假提供有效的鉴定和检测方法,在不同的使用条件下分别实现定性或半定量分析,为建立相关标准提供一定的基础技术支撑。方法:研究采用SYBR GREENⅠ染料实时聚合酶链式反... 目的:本研究致力于建立香辛料真实性成分的分子鉴定方法,为香辛料的掺假提供有效的鉴定和检测方法,在不同的使用条件下分别实现定性或半定量分析,为建立相关标准提供一定的基础技术支撑。方法:研究采用SYBR GREENⅠ染料实时聚合酶链式反应(polymerase chain reaction,PCR)方法,通过设计特异性引物成功建立了特异性检测八角、茴香、胡椒和花椒的分析技术。结果:该方法能够特异性检测出八角、茴香、胡椒、花椒,Ct值在标准品体积分数0.001%~10%范围内线性关系良好。针对八角标准品,检测限为1%;针对茴香、花椒、胡椒标准品,最检测限均为0.001%。利用该方法对5份实际样本进行检测,分别在样本1中未检测出八角、茴香、胡椒、花椒;样本2、3、5中检测出八角、茴香、胡椒;样本4中检测出八角、茴香、胡椒、花椒。结论:本实验建立的SYBR GREENⅠ染料实时PCR方法灵敏度高、重复性好,可应用于实际样品的检测。 展开更多
关键词 香辛料 SYBR greenⅠ染料 实时聚合酶链式反应 检测
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Comparison of growth and nutrient uptake capacities of three dominant species of Qinhuangdao green tides
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作者 Hongbin Han Ruobing Wen +1 位作者 Hui Wang Sheng Zhao 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第4期114-123,共10页
Since 2015, green tides have been blooming in offshore waters of Qinhuangdao, with serious impacts on the local ecological environment and tourism. Ulva australis, Bryopsis plumosa, and U. prolifera are the dominant s... Since 2015, green tides have been blooming in offshore waters of Qinhuangdao, with serious impacts on the local ecological environment and tourism. Ulva australis, Bryopsis plumosa, and U. prolifera are the dominant species of Qinhuangdao green tides, following a sequential succession pattern. Ulva prolifera is the dominant species,with the highest biomass and the greatest influence on the local ecological environment. To study the reason of green tide dominant species succession and U. profilera became the dominant species with the largest biomass,we compared and analyzed the growth and nutrient uptake capacity of the three algae. The results showed that temperature significantly affects the growth of the three species. Within the temperature range of the experimental setup, the optimum temperature for the growth of U. australis, B. plumosa and U. profilera is10℃, 15℃, and 20–25℃, respectively. Combined with the temperature variation trend during green tide bloom development, we believe that temperature is the key environmental factor for the succession of the dominant species. Ulva prolifera has a higher growth rate than U. australis and B. plumosa under the same nitrate,ammonium, and phosphate levels. Significant differences in the maximum absorption rate(R_(max)) and R_(max)/Ks(the relationship between uptake rate and substrate concentration) values indicated that U. prolifera had an apparent competitive advantage over U. australis and B. plumosa regarding nutrient uptake. Therefore, the strong growth and nutrient uptake capacities of U. prolifera might be the main reason for becoming the dominant species with the largest biomass in Qinhuangdao green tides. 展开更多
关键词 GROWTH nutrient uptake dominant specie green tide Qinhuangdao
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SYBR GreenⅠ染料-实时荧光定量聚合酶链式反应法检测发酵乳中的霉菌和酵母含量
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作者 张捷 李献 +4 位作者 张瑞 刘雨蒙 明若阳 陈佳 周巍 《食品安全质量检测学报》 CAS 2024年第16期31-38,共8页
目的建立一种应用SYBR GreenⅠ染料的实时荧光定量聚合酶链式反应法快速检测发酵乳中的霉菌和酵母菌。方法本研究针对霉菌与酵母菌的保守序列设计引物,确定最优反应体系与反应条件。通过熔解曲线以及非目标菌株的检测验证该方法的特异性... 目的建立一种应用SYBR GreenⅠ染料的实时荧光定量聚合酶链式反应法快速检测发酵乳中的霉菌和酵母菌。方法本研究针对霉菌与酵母菌的保守序列设计引物,确定最优反应体系与反应条件。通过熔解曲线以及非目标菌株的检测验证该方法的特异性;通过菌悬液的梯度稀释检测确定该方法的灵敏度;通过菌悬液与发酵乳样品混合后进行检测,确定该方法的检出限,并得到标准曲线。结果该方法能够特异性的检测发酵乳中的霉菌、酵母菌,无交叉反应。该方法检测霉菌、酵母菌的灵敏度均为10^(2) CFU/mL,并且当菌悬液与发酵乳样品混合后,并没有降低该方法的检出限。在10^(2)~10^(6) CFU/mL浓度范围内,菌液浓度的对数值与循环阈值(cyclethreshold,Ct)具有良好的线性关系,可以在市售样品的检测中通过Ct值计算样品中目标菌的含量,更快的判断发酵乳是否被霉菌、酵母菌污染。结论该方法可用于发酵乳中霉菌和酵母菌的快速检测,为发酵乳的食品安全提供了技术保障。 展开更多
关键词 霉菌 酵母 SYBR greenⅠ染料 检测 实时荧光定量聚合酶链式反应 发酵乳
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Superiority of indocyanine green-enhanced near-infrared fluorescence-guided imaging for laparoscopic lymph node dissection in patients with early-stage endometrial cancer: A retrospective cohort study
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作者 Wenzhi Xu Jianqiong Li +3 位作者 Saihua Chen Jiaren Zhang Xueyuan Chen Jianhua Yang 《Laparoscopic, Endoscopic and Robotic Surgery》 2023年第3期103-108,共6页
Objective:Laparoscopic pelvic lymph node dissection(LPND),which is an effective therapy for endometrial cancer,is challenging because of the complexity of the procedure and the occurrence of postoperative complication... Objective:Laparoscopic pelvic lymph node dissection(LPND),which is an effective therapy for endometrial cancer,is challenging because of the complexity of the procedure and the occurrence of postoperative complications.This study aimed to explore whether indocyanine green(ICG)-enhanced nearinfrared(NIR)fluorescence-guided LPND is superior to LPND in the context of early-stage endometrial carcinoma.Methods:In this retrospective study,we included the medical records of 190 patients with early-stage endometrioid adenocarcinoma who underwent LPND at the Department of Obstetrics and Gynecology,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine between January 2019 and January 2021.Depending on whether ICG-enhanced NIR fluorescence guidance was used,the patients were assigned to the ICG group or non-ICG group.Patients were followed-up for one year after surgery.Data on demographic characteristics,pathological results,operative outcomes,and complications were collected and analyzed.Results:The baseline characteristics were comparable between the ICG group and non-ICG group,including age,BMI,pregnancy history,and preoperative hemoglobin.For surgical outcomes,the patients in ICG group had significantly lower intraoperative blood loss(50 mL vs.120 mL,p<0.001),less postoperative pelvic drainage time(4.14±1.44 d vs.5.70±1.89 d,p¼0.001),shorter duration of hospital stay(5.26±1.41 d vs.7.37±1.85 d,p¼0.003),higher number of positive pelvic lymph nodes(PLNs)(1 vs.0,p¼0.003),and more PLN-positive cases(16.0%vs.3.6%,p¼0.003)than the patients in non-ICG group.However,no significant differences were noted in blood transfusion requirement,operative time,hemoglobin level decreases,number of PLNs harvested,or the presence of lymphocysts between the two groups.Conclusion:Our study showed that ICG-enhanced NIR fluorescence-guided operation may improve the accuracy and safety of LPND. 展开更多
关键词 Endometrial cancer Laparoscopic surgery LYMPHADENECTOMY Indocyanine green Near-infrared fluorescence
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Evaluation of Green Development Efficiency in Chongqing Municipality Based on Super-SBM and Malmquist Models
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作者 Zhang Yuefang 《Contemporary Social Sciences》 2023年第5期44-58,共15页
The green development of Chongqing municipality is crucial in establishing a major ecological shield in the upper reaches of the Yangtze River.By developing a Super-SBM model and using the Malmquist index to analyze a... The green development of Chongqing municipality is crucial in establishing a major ecological shield in the upper reaches of the Yangtze River.By developing a Super-SBM model and using the Malmquist index to analyze and calculate the green development efficiency and its influencing factors in Chongqing from 2011 to 2021,this study reveals an accelerating trend in the overall green development efficiency in Chongqing.The significant enhancement of green development efficiency in Chongqing is primarily attributed to changes in returns to scale.Pure technical efficiency and technological advancements have a considerable potential impact on improving green development efficiency in Chongqing.Furthermore,there are discernible disparities in green development efficiency among districts and counties in Chongqing,with different factors influencing these variations.Chongqing is suggested to promote clean and efficient energy utilization,bolster the application and commercialization of scientific and technological advancements,consistently advance ecological restoration and management,and elevate the quality of green development to a higher level. 展开更多
关键词 CHONGQING green development efficiency SupER-EFFICIENCY SBMMalmquist model
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Green Superhydrophobic Paper with Self-cleaning Properties Prepared via One-step Impregnation
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作者 Xiangbin Zhang Shanshan Gao +4 位作者 Xiaoming Song Jiale Wang Xunqian Wu Fushan Chen Shiyuan Xie 《Paper And Biomaterials》 CAS 2023年第4期9-19,共11页
In this study,a green and pollution-free multifunctional superhydrophobic paper-based material was prepared using a simple and efficient dipping method.The superhydrophobic paper with a water contact angle(WCA)of 160&... In this study,a green and pollution-free multifunctional superhydrophobic paper-based material was prepared using a simple and efficient dipping method.The superhydrophobic paper with a water contact angle(WCA)of 160°was prepared by attaching micro-and nanocomposite particles,made of stearic acid-modified chitosan and two kinds of titanium dioxide(TiO_(2))nanoparticles of different sizes,to a paper substrate.The surface morphology,elemental composition,and wetting properties of the coatings were examined using scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FT-IR),and contact angle measurements.Additionally,superhydrophobic coatings exhibited good self-cleaning properties,liquid repellency,ease of repair,and antifouling properties in organic solutions. 展开更多
关键词 SupERHYDROPHOBIC IMPREGNATION green PAPER-BASED SELF-CLEANING
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