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Coupling and coordination relationship of tourism inclusive green growth system:Evidence from Shandong Province
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作者 Tianjun Xu Gangmin Weng Wei Guo 《Chinese Journal of Population,Resources and Environment》 2024年第3期334-342,共9页
Sustainable growth is a prerequisite for high-quality development,and inclusive green growth is regarded as an important way to realize sustainable growth.This paper constructs a tourism inclusive green growth index(I... Sustainable growth is a prerequisite for high-quality development,and inclusive green growth is regarded as an important way to realize sustainable growth.This paper constructs a tourism inclusive green growth index(IGGI)system based on the 2018 Asian Development Bank IGGI.Using Shandong Province from 2017 to 2019,the entropy weight method is utilized to explore the degree of coupling and coordination among the subsystems of tourism inclusive green growth.The study shows that:(1)the tourism IGGI continues to grow;(2)in the tourism inclusive green growth system,the social equity subsystem shows a trend of steady improvement;and(3)in the system,there is a slight discordance between the economic growth subsystem and the environmental sustainability subsystem.The study aims to provide the scientific basis for the high-quality development of tourism and theoretical support for the field of tourism and environmental sustainability. 展开更多
关键词 Tourism inclusive green growth system Coupled coordination Entropy law Tourism quality development
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Tunable dispersion relations manipulated by strain in skyrmion-based magnonic crystals 被引量:1
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作者 金兆年 何宣霖 +3 位作者 于超 方贺男 陈琳 陶志阔 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第1期692-696,共5页
We theoretically investigate the propagation characteristics of spin waves in skyrmion-based magnonic crystals. It is found that the dispersion relation can be manipulated by strains through magneto-elastic coupling. ... We theoretically investigate the propagation characteristics of spin waves in skyrmion-based magnonic crystals. It is found that the dispersion relation can be manipulated by strains through magneto-elastic coupling. Especially, the allowed bands and forbidden bands in dispersion relations shift to higher frequency with strain changing from compressive to tensile,while shifting to lower frequency with strain changing from tensile to compressive. We also confirm that the spin wave with specific frequency can pass the magnonic crystal or be blocked by tuning the strains. The result provides an advanced platform for studying the tunable skyrmion-based spin wave devices. 展开更多
关键词 SKYRMION magnonic crystal spin wave dispersion relation
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Understanding the Relationship Between Shrinking Cities and Land Prices:Spatial Pattern,Effectiveness,and Policy Implications 被引量:1
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作者 WANG Xiaohui PENG Li +1 位作者 HUANG Kexin DENG Wei 《Chinese Geographical Science》 SCIE CSCD 2024年第1期1-18,共18页
Urban shrinkage has emerged as a widespread phenomenon globally and has a significant impact on land,particularly in terms of land use and price.This study focuses on 2851 county-level cities in China in 2005–2018(ex... Urban shrinkage has emerged as a widespread phenomenon globally and has a significant impact on land,particularly in terms of land use and price.This study focuses on 2851 county-level cities in China in 2005–2018(excluding Hong Kong,Macao,Taiwan,and‘no data’areas in Qinhai-Tibet Plateau)as the fundamental units of analysis.By employing nighttime light(NTL)data to identify shrinking cities,the propensity score matching(PSM)model was used to quantitatively examine the impact of shrinking cities on land prices,and evaluate the magnitude of this influence.The findings demonstrate the following:1)there were 613 shrinking cities in China,with moderate shrinkage being the most prevalent and severe shrinkage being the least.2)Regional disparities are evident in the spatial distribution of shrinking cities,especially in areas with diverse terrain.3)The spatial pattern of land price exhibits a significant correlated to the economic and administrative levels.4)Shrinking cities significantly negatively impact on the overall land price(ATT=–0.1241,P<0.05).However,the extent of the effect varies significantly among different spatial regions.This study contributes novel insights into the investigation of land prices and shrinking cities,ultimately serving as a foundation for government efforts to promote the sustainable development of urban areas. 展开更多
关键词 shrinking cities land price propensity score matching(PSM) relative effectiveness China
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Generating Sets of the Complete Semigroup of Binary Relations Defined by Semilattices of the Class Σ8(X, 5)
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作者 Nino Tsinaridze 《Applied Mathematics》 2024年第2期169-197,共29页
In this article, we study generating sets of the complete semigroups of binary relations defined by X-semilattices of unions of the class Σ<sub>8</sub>(X, 5). Found uniquely irreducible generating set for... In this article, we study generating sets of the complete semigroups of binary relations defined by X-semilattices of unions of the class Σ<sub>8</sub>(X, 5). Found uniquely irreducible generating set for the given semigroups and when X is finite set formulas for calculating the number of elements in generating sets are derived. 展开更多
关键词 SEMIGROUP SEMILATTICE Binary relation
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Dispersion Relations in Diffraction in Time
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作者 Salvador Godoy Karen Villa 《Applied Mathematics》 2024年第7期464-468,共5页
In agreement with Titchmarsh’s theorem, we prove that dispersion relations are just the Fourier-transform of the identity, g(x′)=±Sgn(x′)g(x′), which defines the property of being a truncated functions at the... In agreement with Titchmarsh’s theorem, we prove that dispersion relations are just the Fourier-transform of the identity, g(x′)=±Sgn(x′)g(x′), which defines the property of being a truncated functions at the origin. On the other hand, we prove that the wave-function of a generalized diffraction in time problem is just the Fourier-transform of a truncated function. Consequently, the existence of dispersion relations for the diffraction in time wave-function follows. We derive these explicit dispersion relations. 展开更多
关键词 Diffraction in Time Dispersion relations Hilbert Transforms
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Enhancing Data Forwarding Efficiency in SIoT with Multidimensional Social Relations
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作者 Fang Xu Songhao Jiang +3 位作者 Yi Ma Manzoor Ahmed Zenggang Xiong Yuanlin Lyu 《Computers, Materials & Continua》 SCIE EI 2024年第1期1095-1113,共19页
Effective data communication is a crucial aspect of the Social Internet of Things(SIoT)and continues to be a significant research focus.This paper proposes a data forwarding algorithm based on Multidimensional Social ... Effective data communication is a crucial aspect of the Social Internet of Things(SIoT)and continues to be a significant research focus.This paper proposes a data forwarding algorithm based on Multidimensional Social Relations(MSRR)in SIoT to solve this problem.The proposed algorithm separates message forwarding into intra-and cross-community forwarding by analyzing interest traits and social connections among nodes.Three new metrics are defined:the intensity of node social relationships,node activity,and community connectivity.Within the community,messages are sent by determining which node is most similar to the sender by weighing the strength of social connections and node activity.When a node performs cross-community forwarding,the message is forwarded to the most reasonable relay community by measuring the node activity and the connection between communities.The proposed algorithm was compared to three existing routing algorithms in simulation experiments.Results indicate that the proposed algorithmsubstantially improves message delivery efficiency while lessening network overhead and enhancing connectivity and coordination in the SIoT context. 展开更多
关键词 SIoT data forwarding social attributes social relations COMMUNITY
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Defining the We-map Reference Frames and Providing Mathematical Expressions for Transformation Relations
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作者 WANG Xiaolong YAN Haowen +2 位作者 WANG Zhuo MA Wenjun YU Yitao 《Journal of Geodesy and Geoinformation Science》 CSCD 2024年第3期76-88,共13页
We-map is an interactive mobile map that can be easily communicated and applied on personal electronic devices,such as personal computers and mobile phones.Therefore,the study of direction systems and coordinate syste... We-map is an interactive mobile map that can be easily communicated and applied on personal electronic devices,such as personal computers and mobile phones.Therefore,the study of direction systems and coordinate systems is critical,and exploring reference frames is essential in direction and coordinate systems.Despite its significance,existing research on We-map lacks specific solutions for the exploration of reference frames is indispensable for the establishment of accurate direction and coordinate systems.In this paper,we endeavor to address this gap by elucidating the significance of We-map reference frames,defining them with mathematical constraints,summarizing their nature and characteristics,deriving their transformation relationships and representing them through mathematical formulars and equations.Our work contributes to the fundamental theory of We-map and provides valuable systems and support for the mathematical foundation of We-map,map production,and platform development.Ultimately,this research serves to advance the development of We-map. 展开更多
关键词 We-map reference frames spatial direction relation coordinate system
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Relational Turkish Text Classification Using Distant Supervised Entities and Relations
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作者 Halil Ibrahim Okur Kadir Tohma Ahmet Sertbas 《Computers, Materials & Continua》 SCIE EI 2024年第5期2209-2228,共20页
Text classification,by automatically categorizing texts,is one of the foundational elements of natural language processing applications.This study investigates how text classification performance can be improved throu... Text classification,by automatically categorizing texts,is one of the foundational elements of natural language processing applications.This study investigates how text classification performance can be improved through the integration of entity-relation information obtained from the Wikidata(Wikipedia database)database and BERTbased pre-trained Named Entity Recognition(NER)models.Focusing on a significant challenge in the field of natural language processing(NLP),the research evaluates the potential of using entity and relational information to extract deeper meaning from texts.The adopted methodology encompasses a comprehensive approach that includes text preprocessing,entity detection,and the integration of relational information.Experiments conducted on text datasets in both Turkish and English assess the performance of various classification algorithms,such as Support Vector Machine,Logistic Regression,Deep Neural Network,and Convolutional Neural Network.The results indicate that the integration of entity-relation information can significantly enhance algorithmperformance in text classification tasks and offer new perspectives for information extraction and semantic analysis in NLP applications.Contributions of this work include the utilization of distant supervised entity-relation information in Turkish text classification,the development of a Turkish relational text classification approach,and the creation of a relational database.By demonstrating potential performance improvements through the integration of distant supervised entity-relation information into Turkish text classification,this research aims to support the effectiveness of text-based artificial intelligence(AI)tools.Additionally,it makes significant contributions to the development ofmultilingual text classification systems by adding deeper meaning to text content,thereby providing a valuable addition to current NLP studies and setting an important reference point for future research. 展开更多
关键词 Text classification relation extraction NER distant supervision deep learning machine learning
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Do companies influenced by smart cities pay more attention to green governance?Mechanism and optimal spatial correlation
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作者 Xiaoxu Guo Rao Zhang Yue Zhang 《Chinese Journal of Population,Resources and Environment》 2024年第1期48-57,共10页
Smart cities are a way for China to construct an innovative and environmentally conscious nation.The paper examines the impact of smart cities on corporate green governance and provides a theoretical foundation for fo... Smart cities are a way for China to construct an innovative and environmentally conscious nation.The paper examines the impact of smart cities on corporate green governance and provides a theoretical foundation for formulating and executing smart city policy in China.Based on panel data from Chinese A-share listed companies in Shanghai and Shenzhen from 2008 to 2020,this study constructs a multiperiod double-difference model to examine the influence of smart cities on corporate green governance.Additionally,it uses a spatial double-difference model to investigate the spatial spillover effect of smart cities on neighboring areas.The findings indicate that smart cities effectively enhance corporate green governance.Analyzing the influencing mechanisms reveals that resource allocation efficiency,technological innovation,management environmental awareness,and regional environmental enforcement efforts act as mediators.Furthermore,the study reveals that the impact of smart cities on promoting corporate green governance is more pronounced in regions with lower levels of marketization and resource-based cities.Moreover,the research explores the spatial spillover effects of smart cities,with an effective radius of approximately 350 km.The optimal spatial correlation zone for green governance of businesses in neighboring areas in relation to smart cities is within a range of 250-350 km.This is manifested by the significant promotion of green governance in neighboring area businesses facilitated by smart cities. 展开更多
关键词 Smart cities Corporate green governance Mediating effects Spatial associations
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Explanation of Two Important Empirical Relations for Galaxies
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作者 Yingqiu Gu 《Journal of Applied Mathematics and Physics》 2024年第1期284-304,共21页
The phenomenon of “missing mass” in galaxies has triggered new theoretical exploration, forming a competition between dark matter assumption, modified Newtonian dynamics and modified gravity. Over the past forty yea... The phenomenon of “missing mass” in galaxies has triggered new theoretical exploration, forming a competition between dark matter assumption, modified Newtonian dynamics and modified gravity. Over the past forty years, various versions of the modified scenario have been proposed to simulate the effects of missing mass. These schemes replace the dynamic effect of dark matter by introducing some tiny extra force terms in the dynamic equations. Such extra forces have mainly interactions on large scales of galaxies, such as fitting the Tully-Fisher relation or asymptotically flat rotation curves. The discussion in this paper shows that the evidence of taking the modified schemes as fundamental theory is still insufficient. In this paper, we display a system of simplified galactic dynamical equations derived from weak field and low-speed approximations of Einstein field equations, and then we use it to discuss two important empirical relations in galactic dynamics, namely the Faber-Jackson relation and Tully-Fisher relation, as well as the related fundamental plane. These discussions provide a reference scheme for improving the dispersion of the empirical relations, and also provide a theoretical foundation to analyze the properties of dark matter and galactic structures. 展开更多
关键词 Galactic Dynamics Faber-Jackson relation Tully-Fisher relation Dark Matter MOND
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Relationship between the Ecological Livability and the Green Development of Agriculture
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作者 Wenhui LI Hongyang LI Xingxing SUN 《Agricultural Biotechnology》 CAS 2023年第4期85-87,共3页
Ecological livability is one of the important tasks and objectives of the rural revitalization strategy proposed at the 19 th National Congress of the CPC.The most precious wealth of the countryside is the excellent e... Ecological livability is one of the important tasks and objectives of the rural revitalization strategy proposed at the 19 th National Congress of the CPC.The most precious wealth of the countryside is the excellent ecological environment,and China must unswervingly follow the route of green agricultural development.Based on current research on the relationship between ecological livable and agricultural green development,comprehensive policy documents,journal literature and related reports,this paper explored the background and connotation of ecological livable,the current situation of agricultural green development and existing problems,and profoundly analyzed ecological livable and the internal connection between agricultural green development,aiming to provide theoretical reference and scientific reference for the final realization of ecological livable goal. 展开更多
关键词 Ecological and livable Agricultural green agricultural development Rural revitalization
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Exploring the spatiotemporal relationship between green infrastructure and urban heat island under multi‐source remote sensing imagery:A case study of Fuzhou City
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作者 Tingting Hong Xiaohui Huang +2 位作者 Guangjian Chen Yiwei Yang Lijia Chen 《CAAI Transactions on Intelligence Technology》 SCIE EI 2023年第4期1337-1349,共13页
Green Infrastructure(GI)has garnered increasing attention from various regions due to its potential to mitigate urban heat island(UHI),which has been exacerbated by global climate change.This study focuses on the cent... Green Infrastructure(GI)has garnered increasing attention from various regions due to its potential to mitigate urban heat island(UHI),which has been exacerbated by global climate change.This study focuses on the central area of Fuzhou city,one of the“furnace”cities,and aims to explore the correlation between the GI pattern and land surface temperature(LST)in the spring and autumn seasons.The research adopts a multiscale approach,starting from the urban scale and using urban geographic spatial characteristics,multispectral remote sensing data,and morphological spatial pattern analysis(MSPA).Significant MSPA elements were tested and combined with LST to conduct a geographic weighted regression(GWR)experiment.The findings reveal that the UHI in the central area of Fuzhou city has a spatial characteristic of“high temperature in the middle and low temperature around,”which is coupled with a“central scattered and peripheral concentrated”distribution of GI.This suggests that remote sensing data can effectively be utilised for UHI inversion.Additionally,the study finds that the complexity of GI,whether from the perspective of the overall GI pattern or the classification study based on the proportion of the core area,has an impact on the alleviation of UHI in both seasons.In conclusion,this study underscores the importance of a reasonable layout of urban green infrastructure for mitigating UHI. 展开更多
关键词 geographic weighted regression green infrastructure image analysis morphological spatial pattern analysis statistical analysis urban heat island
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盆底超声检查和Green分型诊断产妇产后早期盆底结构及压力性尿失禁 被引量:1
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作者 黄雪 周京晶 +3 位作者 李梦妮 白炜 容亚娟 邵越 《中国计划生育学杂志》 2024年第6期1444-1447,共4页
目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsa... 目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsalva动作状态下膀胱颈距耻骨联合下缘的距离(BSD)、膀胱尿道后角(PUA)、膀胱颈移动度(BND)和尿道旋转角(UR),对比Green分型;采用受试者工作特征(ROC)曲线分析相关参数诊断SUI价值。结果:静息状态下,两组盆底超声参数无差异(P>0.05);SUI组Valsalva动作状态下的BSD、PUA、BND和UR值均大于非SUI组,Green分型Ι型占比(25.6%)低于非SUI组(59.8%)(均P<0.05)。ROC曲线分析,Green分型曲线下面积(AUC)0.579,对SUI诊断价值不佳(P=0.057),Valsalva动作状态下BSD(AUC=0.758)、PUA(AUC=0.743)、BND(AUC=0.769)和UR(AUC=0.705)对SUI诊断价值良好(均P<0.05)。结论:盆底超声检查和Green分型能有效评估经产妇早期盆底结构状况,前者可诊断SUI。 展开更多
关键词 压力性尿失禁 产后早期 盆底结构 超声检查 green分型 诊断
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水禽细小病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立与应用
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作者 汪宏才 商雨 +7 位作者 马瑶 曾哲 张蓉蓉 姚伦 罗玲 李丽 温国元 罗青平 《湖北农业科学》 2024年第6期218-222,共5页
为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.... 为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.77;除WPV出现S形扩增曲线外,新城疫病毒(NDV)、H9亚型禽流感病毒(H9 AIV)、鸭坦布苏病毒(DTMUV)、鸭肝炎病毒(DHAV)、鸭肠炎病毒(DEV)、鸭呼肠孤病毒(DRV)样品均未出现S形阳性扩增曲线;批内变异系数(CV)为0.15%~0.23%,批间变异系数为0.09%~0.28%。结果表明,SYBR Green Ⅰ荧光定量PCR检测方法重复性好、灵敏度高和特异性强。临床样品检测结果表明,SYBR Green Ⅰ荧光定量PCR与普通PCR的符合率达98.4%,灵敏度是普通PCR的1 000倍。SYBR Green Ⅰ荧光定量PCR检测方法不仅能定性检测WPV,还可以进行定量检测,可用于种鸭场、种鹅场的WPV净化检测,也可用于WPV临床大量样品的快速检测。 展开更多
关键词 水禽细小病毒 检测方法 SYBR green 荧光定量PCR
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约束Green函数与非线性地基梁模态分析 被引量:1
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作者 赵翔 王琦 +1 位作者 朱伟东 李映辉 《动力学与控制学报》 2024年第2期77-84,共8页
在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础... 在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础上的梁振动模型,研究了任意位置弹簧和非线性弹簧基础上的梁模态.通过Laplace变换和线性叠加原理,得到了一种约束Green函数,利用数值计算验证方案的有效性,并研究了各种重要物理参数的影响,发现弹簧位置向跨中移动时,模态对称性被打破,弹簧刚度增加,模态阶数改变. 展开更多
关键词 约束green函数 LAPLACE变换 非线性弹簧基础 模态分析 叠加原理
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美洲鳗鲡腺瘤病毒(AEAdoV)普通PCR和SYBR Green I实时荧光定量PCR检测方法的建立及应用 被引量:1
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作者 孔文迪 陈曦 +1 位作者 杨金先 葛均青 《水产学报》 CAS CSCD 北大核心 2024年第4期358-365,共8页
为建立美洲鳗鲡腺瘤病毒(AEAdoV)的检测方法,根据AEAdoV福建株(AEAdoVFJ)的superfamily 3 helicases(S3H)序列,设计引物,建立了AEAdoV的普通PCR和qPCR检测方法;进一步评价检测方法的灵敏性、特异性及重复性,利用2种方法对美洲鳗鲡“出... 为建立美洲鳗鲡腺瘤病毒(AEAdoV)的检测方法,根据AEAdoV福建株(AEAdoVFJ)的superfamily 3 helicases(S3H)序列,设计引物,建立了AEAdoV的普通PCR和qPCR检测方法;进一步评价检测方法的灵敏性、特异性及重复性,利用2种方法对美洲鳗鲡“出血性烂鳃”病料进行了检测,并对美洲鳗鲡体内不同组织的病毒含量进行分析。结果显示,普通PCR扩增的目的片段长度约300 bp,利用其构建的qPCR质粒标准品,其拷贝数与qPCR阈值循环数(C_(t))线性关系良好,线性范围广,标准曲线相关系数(R2)达到0.999,扩增效率为105.067%。建立的普通PCR法和qPCR的最低检测AEAdoV拷贝数分别为100个和10个。2种方法均可特异性检测AEAdoV,而对蛙虹彩病毒(RGV)、鳗鲡疱疹病毒(AngHV)、鲤疱疹病毒(KHV)、对虾白斑综合征病毒(WSSV)、日本鳗鲡内皮细胞坏死病毒(JEAdoV)和花鳗鲡腺瘤病毒(MEAdoV)均无扩增反应。qPCR法的组内和组间变异系数均小于2%,表明其重复性良好。临床应用结果显示,35份美洲鳗鲡“出血性烂鳃”病料,采用普通PCR法的AEAdoV检出率为82.8%,而qPCR法的AEAdoV检出率为97%。对美洲鳗鲡不同组织的病毒含量分析结果显示,心脏、肝脏、鳃、鳍的AEAdoV相对含量较高,而黏液、皮肤和脾脏的病毒含量相对较低。研究表明,建立的AEAdoV的灵敏度高、特异性强的普通PCR和qPCR检测方法,证实AEAdoV与美洲鳗鲡“出血性烂鳃”病密切相关,且在感染鳗鲡主要组织中都存在。实验结果对于研究AEAdoV的致病性,开展其流行情况和病原学情况分析具有重要意义。 展开更多
关键词 美洲鳗鲡腺瘤病毒(AEAdoV) 普通PCR 荧光定量PCR SYBR green 检测方法
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3型哺乳动物正呼肠孤病毒SYBR Green I荧光定量PCR方法的建立及应用
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作者 汤文菲 罗宇航 +12 位作者 董覃婷 朱鑫玥 王杨林 韦祖樟 陈樱 欧阳康 覃一峰 钟舒红 谢江 陈集成 王小玲 黄伟坚 潘艳 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第8期819-824,共6页
哺乳动物正呼肠孤病毒(MRV)是双链RNA病毒,可以感染自然宿主的哺乳动物和脊椎动物。为建立一种针对3型哺乳动物正呼肠孤病毒(MRV-3)的特异且快速的检测方法,本研究根据Genbank登录的MRV-3(OQ627746-OQ627755)S1基因保守区设计1对特异性... 哺乳动物正呼肠孤病毒(MRV)是双链RNA病毒,可以感染自然宿主的哺乳动物和脊椎动物。为建立一种针对3型哺乳动物正呼肠孤病毒(MRV-3)的特异且快速的检测方法,本研究根据Genbank登录的MRV-3(OQ627746-OQ627755)S1基因保守区设计1对特异性引物,并从MRV-3中扩增S1基因,构建重组质粒p MD18-S1,经PCR和测序鉴定正确后作为质粒标准品,经反应体系及反应条件优化后首次初步建立了检测MRV-3的SYBR Green I荧光定量PCR(q PCR)方法。将质粒标准品10倍倍比稀释后作为模板经该q PCR扩增,建立标准曲线,结果显示,质粒标准品在1.3×10^(8)拷贝/μL~1.3×10^(3)拷贝/μL与各自的Ct值均呈良好的线性关系,斜率为-3.1706,R^(2)为0.9999,熔解曲线为单峰。以牛病毒性腹泻病毒(BVDV)、牛肠道病毒(BEV)、水牛匈爱病毒(Buf Hu V)、牛冠状病毒(BCo V)、牛细小病毒(BPV)和MRV-3的基因组DNA/c DNA为模板,利用本研究建立的q PCR方法检测,评估该方法的特异性;将质粒标准品10倍倍比稀释至1.3×10^(2)拷贝/μL~1.3×10^(8)拷贝/μL后作为模板,分别利用本研究建立的q PCR和常规PCR检测,比较两种方法的检测结果,评估本研究建立q PCR方法的敏感性;以1.3×10^(3)拷贝/μL~1.3×10^(7)拷贝/μL 5个不同浓度的质粒标准品为模板,利用该方法分别进行批内和批间的重复性试验,评估该方法的重复性。结果显示,该方法只能检测出MRV-3,其他相关病原的检测结果均为阴性;该q PCR对质粒标准品的检测限为1.3×10^(3)拷贝/μL,比常规PCR敏感性高10 000倍;批内和批间重复性试验的变异系数均小于或等于1.0%,表明该q PCR方法特异性强、敏感性高、重复性好。利用该方法检测220份牛粪便样品,结果显示MRV-3的检出率(3.64%,8/220)高于常规PCR的检出率(1.36%,3/220),两种检测方法的阳性符合率达100%,阴性符合率为97.75%,总符合率为97.78%。综上所述,本研究首次建立的检测MRV-3的SYBR Green I q PCR方法可以用于临床牛腹泻病原的检测,为MRV-3尤其是牛源MRV-3提供了一种快速灵敏的检测手段,也为MRV-3的后续研究奠定了基础。 展开更多
关键词 哺乳动物正呼肠孤病毒 SYBR green I 荧光定量PCR 病毒检测
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猪流行性腹泻病毒SYBR Green I实时荧光定量PCR检测方法的建立
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作者 董苏洁 孔宁 +9 位作者 秦文珍 翟焕杰 翟雪滢 杨心雨 童武 郑浩 于海 童光志 李有文 单同领 《中国动物传染病学报》 CAS 北大核心 2024年第2期174-180,共7页
为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。... 为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。 展开更多
关键词 猪流行性腹泻病毒 N基因 SYBR green I 荧光定量PCR
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三疣梭子蟹十足目虹彩病毒1 SYBR Green I荧光定量PCR检测方法的建立及初步应用
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作者 赵丹阳 施慧 +2 位作者 许文军 何杰 王庚申 《海洋与湖沼》 CAS CSCD 北大核心 2024年第5期1273-1281,共9页
为建立十足目虹彩病毒1(decapod iridescent virus 1,DIV1)的SYBR GreenⅠ荧光定量PCR检测方法,根据DIV1的MCP和ATPase基因序列,设计并筛选出引物,以制备的DIV1阳性质粒标准品为模板构建标准曲线,建立DIV1的SYBR Green I qPCR方法,并对... 为建立十足目虹彩病毒1(decapod iridescent virus 1,DIV1)的SYBR GreenⅠ荧光定量PCR检测方法,根据DIV1的MCP和ATPase基因序列,设计并筛选出引物,以制备的DIV1阳性质粒标准品为模板构建标准曲线,建立DIV1的SYBR Green I qPCR方法,并对该方法进行临床初步应用。结果显示,建立的qPCR方法阈值循环数(cycle threshold value,Ct)与标准品拷贝数的对数线性关系良好,标准曲线相关系数(R^(2))为0.999;对DIV1阳性的虾蟹核酸样本能够进行特异性扩增,但对传染性脾肾坏死病毒(infectious spleen and kidney necrosis virus,ISKNV)和白斑综合征病毒(white spot syndrome virus,WSSV)阳性核酸样本均无扩增;最低检测限为9.77 copies/μL;Ct值的组内和组间变异系数均小于1%。运用该方法对70份疑似感染DIV1的虾蟹类样本进行DIV1检测,该方法阳性率为48.57%,与套式PCR检测方法的阳性率一致;利用建立的方法对DIV1阳性三疣梭子蟹的血淋巴、肝胰腺及心脏等组织进行定量检测分析,结果显示各组织中均存在DIV1,其中血淋巴中DIV1平均拷贝数最高。研究表明,建立的SYBR GreenⅠ荧光定量PCR方法特异性强、灵敏度高、重复性好,可用于对DIV1的快速、定量检测,对十足目虹彩病毒病的诊断和防控具有重要意义。 展开更多
关键词 十足目虹彩病毒1(decapod iridescent virus 1 DIV1) 荧光定量PCR SYBR green 检测方法
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(Generalized)Green's Relations of GV-Semigroups 被引量:1
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作者 张建刚 申冉 《Journal of Donghua University(English Edition)》 EI CAS 2012年第2期175-177,共3页
Green's relations and generalized Green's relations play a fundamental role in the study of semigroups.GV-semigroups are the generalizations of completely regular semigroups in the range of π-regular semigrou... Green's relations and generalized Green's relations play a fundamental role in the study of semigroups.GV-semigroups are the generalizations of completely regular semigroups in the range of π-regular semigroups.In this paper,Green's relations and generalized Green's relations on GV-semigroups are considered by the structure of GV-semigroups.D=j and D C D* on GV-semigroups will be proved. 展开更多
关键词 GV-SEMIGROUP GV-inverse semigroup green’s relations
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