Tumor-Specific Histo-Blood Group Antigens: Apropos of Two Cases

Cancer cells with immunogenic properties having altered protein glycosylation, modified blood group substances have been widely studied. Due to the genetic instability occurring during carcinogenesis the glycosyltransferases may suffer from posttranslation sequence modification. The author describes 2 autopsy cases, where in the background of the unusual metastatic tumor presentation, incompatible blood group antigenic determinants have been demonstrated using blood group specific lectins and monoclonal antibodies (mAb). In the first case, reported here, a 10-year-old girl developed an acute myeloid leukemia and died in a septic endotoxin shock after successful cytostatic treatment of a juvenile signet ring cell cancer of her colon. At autopsy there were no signs of tumor except bilateral apple-sized mucinous ovarian (Krukenberg) metastases. While she had erythrocyte phenotype of blood group A, the signet ring adenocarcinoma cells expressed blood group B incompatible antigenic determinants with lectin/mAb. In the second case, the autopsy of a 78-year-old female resulted in no macroscopic tumor sign except a moderately enlarged, ham hard spleen. Light microscopy revealed adenocarcinomatous infiltration in the splenic sinusoids. The patient had blood group O, while the metastatic cells in the spleen reacted with Breast Carcinoma Antigen (BioGenex) and incompatible anti-B Banderiaeasimplicifolia agglutinin I and anti-B mAb. It proved to be a case of an occult, completely regressed breast cancer. Based on these observations the expression of tumor specific incompatible blood group antigens might occur from time to time, mostly in adenocarcinomas. Accordingly, blood group-based specific immuno-oncotherapy could be considered in some cancer cases.

Enterprise Internal Control Based on Risk Management with Moutai Group as an Example

Focusing on the two themes of"internal control"and"risk management",this paper makes an in-depth analysis of the current situation of risk management and internal control of Moutai Group.It analyzes the current situation of risk management and internal control of Moutai Group.It is found that the risk management of Moutai Group is not perfect,the information exchange is not smooth,and the internal control assessment is not perfect.Finally,it puts forward some corresponding countermeasures,including establishing an effective information communication mechanism,perfecting the risk assessment system,and strengthening the construction of the professional talent team.

RELATIVE ENTROPY DIMENSION FOR COUNTABLE AMENABLE GROUP ACTIONS

We study the topological complexities of relative entropy zero extensions acted upon by countable-infinite amenable groups.First,for a given Følner sequence,we define the relative entropy dimensions and the dimensions of the relative entropy generating sets to characterize the sub-exponential growth of the relative topological complexity.we also investigate the relations among these.Second,we introduce the notion of a relative dimension set.Moreover,using the method,we discuss the disjointness between the relative entropy zero extensions via the relative dimension sets of two extensions,which says that if the relative dimension sets of two extensions are different,then the extensions are disjoint.

Improvisation of Node Mobility Using Cluster Routing-based Group Adaptive in MANET

In today's Internet routing infrastructure,designers have addressed scal-ing concerns in routing constrained multiobjective optimization problems examining latency and mobility concerns as a secondary constrain.In tactical Mobile Ad-hoc Network(MANET),hubs can function based on the work plan in various social affairs and the internally connected hubs are almost having the related moving standards where the topology between one and the other are tightly coupled in steady support by considering the touchstone of hubs such as a self-sorted out,self-mending and self-administration.Clustering in the routing process is one of the key aspects to increase MANET performance by coordinat-ing the pathways using multiple criteria and analytics.We present a Group Adaptive Hybrid Routing Algorithm(GAHRA)for gathering portability,which pursues table-driven directing methodology in stable accumulations and on-request steering strategy for versatile situations.Based on this aspect,the research demonstrates an adjustable framework for commuting between the table-driven approach and the on-request approach,with the objectives of enhancing the out-put of MANET routing computation in each hub.Simulation analysis and replication results reveal that the proposed method is promising than a single well-known existing routing approach and is well-suited for sensitive MANET applications.

Conditional Similarity Reductions of Jimbo—Miwa Equation via the Classical Lie Group Approach

Recently, the Clarkson and Kruskal direct method has been modified to find new similarity reductions (conditional similarity reductions) of nonlinear systems and the results obtained by the modified direct method cannot be obtained by the current classical and/or non-classical Lie group approach. In this paper, we show that the conditional similarity reductions of the Jimbo-Miwa equation can be reobtained by adding an additional constraint equation to the original model to form a conditional equation system first and then solving the model system by means of the classical Lie group approach.

Identification and Characterization of Peptide Mimics of Blood Group A Antigen

In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase （GST） fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A antigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.

Molecular Epidemiological Analysis of Group A Streptococci Isolated from Children in Chaoyang District of Beijing, 2011:emm Types, Virulence Factor Genes and Erythromycin Resistant Genes

Group A streptococcus （GAS） causes a wide range of diseases in the human population. GAS diseases are more common in children than in adults, with clinical manifestations ranging from pharyngitis and impetigo to invasive infections and post streptococcal sequelae, such as acute rheumatic fever and acute post-streptococcal glomerulonephritis[1]. GAS harbors a host of virulence factors that contribute to its complex pathogenicity and differences in the disease severity and frequency. M protein, one of the major virulence factors, is encoded by the emm gene induces a type of specific host immune response and confers antiphagocytic properties.

Histo-blood group antigens in Crassostrea gigas and binding profiles with GⅡ.4 Norovirus

Noroviruses(NoVs) are the main cause of viral gastroenteritis outbreaks worldwide, and oysters are the most common carriers of NoV contamination and transmission. NoVs bind specifically to oyster tissues through histo-blood group antigens(HBGAs), and this facilitates virus accumulation and increases virus persistence in oysters. To investigate the interaction of HBGAs in Pacific oysters with GⅡ.4 NoV, we examined HBGAs with ELISAs and investigated binding patterns with oligosaccharide-binding assays using P particles as a model of five GⅡ.4 NoV capsids. The HBGAs in the gut and gills exhibited polymorphisms. In the gut, type A was detected(100%), whereas type Leb(91.67%) and type A(61.11%) were both observed in the gills. Moreover, we found that seasonal NoV gastroenteritis outbreaks were not significantly associated with the specific HBGAs detected in the oyster gut and gills. In the gut, we found that strain-2006 b and strain-96/96 US bound to type A and H1 but only weakly bound to type Leb; in contrast, the Camberwell and Hunter strains exhibited weak binding to types H1 and Ley, and strain-Sakai exhibited no binding to any HBGA type. In the gills, strain-96/96 US and strain-2006 b bound to type Leb but only weakly bound to type H1; strains Camberwell, Hunter, and Sakai did not bind to oyster HBGAs. Assays for oligosaccharide binding to GⅡ.4 NoV P particles showed that strain-95/96 US and strain-2006 b strongly bound to type A, B, H1, Leb, and Ley oligosaccharides, while strains Camberwell and Hunter showed weak binding ability to type H1 and Ley oligosaccharides and strain-Sakai showed weak binding ability to type Leb and Ley oligosaccharides. Our study presents new information and enhances understanding about the mechanism for NoV accumulation in oysters. Further studies of multiple NoV-tissue interactions might assist in identifying new or improved strategies for minimizing contamination, including HBGA-based attachment inhibition or depuration.

MULTIPLICATIVE GROUP AUTOMORPHISMS OF INVERTIBLE UPPER TRIANGULAR MATRICES OVER FIELDS

Suppose F is a field of characteristic not 2 and F* its multiplicative group. Let T*n(F) be the multiplicative group of invertible upper triangular n x n matrices over F and STn(F) its subgroup {(aij) E T*n(F)aii = 1, i}. This paper proves that f: T*n(F) → T*n(F) is a group automorphism if and only if there exist a matrix Q in T*n(F) and a field automorphism rs of F such that either where A = ((aij)), A-T is the transpose inverse of A, J = Ei n+1-i, and : i= 1T*n(F) → F* is a homomorphism which satisfies {(xIn)(x)x F*} = F* and {x F*(xIn)(x) = 1} = {1}. Simultaneously, they also determine the automorphisms of STn(F).

Norovirus Infection and Histo-blood Group Antigens in Children Hospitalized with Diarrhea in Lulong and Chenzhou in China

Norovirus （NOV） is a pathogen that commonly causes viral diarrhea in children. Studies indicate that NoV recognizes human histo-blood group antigens （HBGAs） as cell attachment factors. In order to explore the correlation between of NoV infection and HBGAs, a cross-sectional study was conducted in children less than five years old who were hospitalized with diarrhea in two areas of China between November 2014 and February 2015. Of the paired stool and saliva samples taken from 424 children,

New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus

An effective vaccine against group A streptococcus(GAS)is highly desirable for definitive control of GAS infections.In the present study,two variants of amphiphilic chitosan nanoparticles-based GAS vaccines were developed.The vaccines were primarily composed of encapsulated KLH protein(a source of T helper cell epitopes)and lipidated M-protein derived B cell peptide epitope(lipoJ14)within the amphiphilic structure of nanoparticles.The only difference between themwas one of the nanoparticles vaccines received additional surface coating with poly(I:C).The formulated vaccines exhibited nanosized particles within the range of 220–240 nm.Cellular uptake study showed that nanoparticles vaccine without additional poly(I:C)coating has greater uptake by dendritic cells and macrophages compared to nanoparticles vaccine that was functionalized with poly(I:C).Both vaccines were found to be safe in mice and showed negligible cytotoxicity against HEK293 cells.Upon immunization in mice,both nanoparticle vaccines produced high antigen-specific antibodies titres that were regulated by a balanced Th1 and Th2 response compared to physical mixture.These antibodies elicited high opsonic activity against the tested GAS strains.Overall,our data demonstrated that amphiphilic chitosan nanoparticles platform induced a potent immune response even without additional inclusion of poly(I:C).

The efficacy of the inhalation of an aerosolized Group A streptococcal preparation in the treatment of lung cancer

Objective： To observe the efficacy of the inhalation of an aerosonzeo group ~ sueloto^u v / preparation in treating orthotopic lung cancer in mouse models and assess the feasibility, safety, and effectiveness of this administration mode for lung cancer. Methods： Lewis lung carcinoma （LLC） cell strains were administered via intrathoracic injection to establish orthotopic lung cancer mouse models. After the tumor-bearing models were successfully established, as confirmed by computed tomography, the mice were administered by inhalation with an aerosolized GAS preparation （GAS group） or aerosolized normal saline （control group）. The anti-tumor effect of the aerosolized GAS preparation was evaluated histologically; meanwhile, the survival and quality of life were compared between these two groups. Results： The aerosolized GAS preparation showed remarkably anti-tumor effect, causing the necrosis of the orthotopic lung cancer cells in tumor-bearing mice. Furthermore, mice in the GAS group had significantly better qualitT of life and longer survival than those in control group. Conclusions： The inhalation of aerosolized GAS preparation may be a feasible, safe and effective solution for lung cancer

Matrix Elements of One— and Two—Body Operators in the Unitary Group Approach （Ⅱ）—Application

Simple analytical expressions for one- and two-body matrix elements in the unitary group approach to the configuration interaction problems of many-electron systems are obtained based on the previous results for general irreps.

Test analysis of stress characteristics on reinforcing rock slope with group anchorage cable

To research the reinforcement effect of a rock slope with group anchorage cables and the stress characteristics of pre-stressed anchorage cables in the fractured surface, the rock slope calculated model of a wedge block within the double-slide face was established by using the finite difference software according to the actual slope project combined with indoor model test. The pre-stress loss rule of the anchorage cable and the distribution of axial force and the force-transferring mechanism of the anchorage cable were analyzed during simulation. Also, based on the displacement contour and the safety factor of the calculated results, the quantitative analysis for the reinforcement ef- fect of the rock slope with group pre-stressed anchorage cable was discovered. The results computed by the software conform with the data in the experiment, which can prove the effectiveness and correctness of parameter selection and model building. Keywords group anchorage reinforcement, rock slope, joint fissure, pre-stressed anchorage cable, stress characteristics

Group A streptococcal antigen exposed rat model to investigate neurobehavioral and cardiac complications associated with post-streptococcal autoimmune sequelae

Background:The neuropsychiatric disorders due to post-streptococcal autoimmune complications such as Sydenham's chorea(SC)are associated with acute rheumatic fever and rheumatic heart disease(ARF/RHD).An animal model that exhibits char-acteristics of both cardiac and neurobehavioral defects in ARF/RHD would be an important adjunct for future studies.Since age,gender,strain differences,and geno-types impact on the development of autoimmunity,we investigated the behavior of male and female Wistar and Lewis rat strains in two age cohorts(6 weeks and 12 weeks)under normal husbandry conditions and following exposure to group A streptococcus(GAS).Methods:Standard behavioral assessments were performed to determine the impair-ments in fine motor control(food manipulation test),gait and balance(beam walk-ing test),and obsessive-compulsive behavior(grooming and marble burying tests).Furthermore,electrocardiography,histology,and behavioral assessments were per-formed on male and female Lewis rats injected with GAS antigens.Results:For control Lewis rats there were no significant age and gender dependent differences in marble burying,food manipulation,beam walking and grooming be-haviors.In contrast significant age-dependent differences were observed in Wistar rats in all the behavioral tests except for food manipulation.Therefore,Lewis rats were selected for further experiments to determine the effect of GAS.After ex-posure to GAS,Lewis rats demonstrated neurobehavioral abnormalities and cardiac pathology akin to SC and ARF/RHD,respectively.Conclusion:We have characterised a new model that provides longitudinal stability of age-dependent behavior,to simultaneously investigate both neurobehavioral and cardiac abnormalities associated with post-streptococcal complications.

FUNDAMENTAL GROUPS OF CLOSED POSITIVELY CURVED MANIFOLDS WITH ALMOST DISCRETE ABELIAN GROUP ACTIONS

Let M be a closed n-manifold of positive sectional curvature. Assume that M admits an effective isometrical T1× Zpk-action with p prime. The main result of the article n＋1 for n 〉 5, then there exists a positive constant p（n）, is that ifk=lforn=3or k〉 n＋1/4 for n≥5,then there exists a positive constant p（n）,depending only on n, such that π1 （M） is cyclic if p ≥ p（n）.

On the Structure of the Units of Group Algebra of Dihedral Group

In this paper, we completely determine the structure of the unit group of the group algebra of some dihedral groups D2 n over the finite field Fpk, where p is a prime.

CHAOTIC GROUP ACTIONS

It is proved that there is no chaotic group actions on any topological space with free arc.In this paper the chaotic actions of the group like G×F ,where F is a finite group,are studied.In particular,under a suitable assumption,if F is a cyclic group,then the topological space which admits a chaotic action of Z×F must admit a chaotic homeomorphism.A topological space which admits a chaotic group action but admits no chaotic homeomorphism is constructed.

LOCAL AUTOMORPHISMS OF SEMISIMPLE ALGEBRAS AND GROUP ALGEBRAS

Let F be a field of characteristic not 2, and let A be a finite-dimensional semisimple F -algebra. All local automorphisms of A are characterized when all the degrees of A are larger than 1. If F is further assumed to be an algebraically closed field of characteristic zero, K a finite group, F K the group algebra of K over F , then all local automorphisms of F K are also characterized.

A Dynamic Active Multicast Group Access Control Framework Based on Trust Management System

The current multicast model provides no access control mechanism. Any host can send data directly to a multicast address or join a multicast group to become a member, which brings safety problems to multicast. In this paper, we present a new active multicast group access control mechanism that is founded on trust management. This structure can solve the problem that exists in multicast members＇ access control and distributing authorization of traditional IP multicast.