Blood group type antigens in pancreatic intraductal papillary mucinous neoplasms

BACKGROUND: There are few data on blood group(BG) types and types of pancreatic cancers. The aims of this study were to study BG types and BG-antigens in pancreatic intraductal papillary mucinous neoplasms(IPMNs). METHODS: BG type and tumor BG-antigen(glycoprotein) expression(studied by immunohistochemistry on tissue microarrays) were analyzed with regard to characteristics of 101 surgically resected pancreatic IPMNs. RESULTS: Non-O BG type predicted invasive carcinoma independently from high serum CA19-9 and male gender. BG type A was observed more frequently in women than in men. Chronic pancreatitis was more frequently seen in patients with BG type B or AB. Aberrant tumor expression(with regard to BG type) of loss of A antigen expression type occurred in 15.0% of IPMNs and of loss of B antigen expression type in 62.5% of IPMNs. Intraneoplasm BG-antigen expression was not related to dysplasia grade or invasion. CONCLUSION: The results of the study suggest that in pancreatic IPMN, non-O BG type predicted invasive carcinoma, whereas for intratumor BG-antigen expression no specific patterns were detected with regard to the progression of glandular epithelial dysplasia or invasion.

Group of Weakly Continuous Operators Associated to a Generalized Schrödinger Type Homogeneous Model

In this work, we prove the existence and uniqueness of the solution of the generalized Schrödinger type homogeneous model in the periodic distributional space P’. Furthermore, we prove that the solution depends continuously respect to the initial data in P’. Introducing a family of weakly continuous operators, we prove that this family is a group of operators in P’. Then, with this family of operators, we get a fine version of the existence and dependency continuous theorem obtained. Finally, we give some remarks derived from this study.

IMPROVED GAGLIARDO-NIRENBERG INEQUALITIES ON HEISENBERG TYPE GROUPS

Motivated by the idea of M. Ledoux who brings out the connection between Sobolev embeddings and heat kernel bounds, we prove an analogous result for Kohn’s sub-Laplacian on the Heisenberg type groups. The main result includes features of an inequality of either Sobolev or Galiardo-Nirenberg type.

LOCAL ESTIMATE ABOUT SCHRODINGER MAXIMAL OPERATOR ON H-TYPE GROUPS

Let △ be full Laplacian on H-type group G. Then for every compact set D Ga local estimate of the Schrodinger maximal operator holds, that is,∫D^sup0〈t〈1｜e^it△f（x）｜^2dx≤｜｜f｜｜^2H^s,s〉1/2We also show that the above inequality fails when s 〈 1/4.

The Features of Sedimentary Facies and Copper Enrichment Metallogenic Regularities of Kuzigongsu Group in Sareke Glutenite Type Copper Deposits,Wuqia,Xinjiang

1 Introduction Sareke glutenite-type copper deposit is the large size copper deposit discovered in recent years,and it is located Sarekebayi intracontinental pull-apart basin in the western margin of the Tarim basin.Conglomerate of

HEAT KERNELS AND HARDY'S UNCERTAINTY PRINCIPLE ON H-TYPE GROUPS

This article obtains an explicit expression of the heat kernels on H-type groups and then follow the estimate of heat kernels to deduce the Hardy＇s uncertainty principle on the nilpotent Lie groups.

Blood groups, hemoglobin phenotypes and clinical disorders of consanguineous Yansi population

AIM:To study frequency of blood groups,prevalence of sickle-cell anemia trait and glucose-6-phosphate dehydrogenase deficiency(G6PD),among consanguineous Yansi tribe.METHODS:A total of 525 blood samples were collected,of which 256 among the Yansi population,and269 for the unrelated control group in the Bandunduprovince of Democratic Republic of Congo.Blood group antigens were determined in the following systems:ABO,Rh,Kell,Duffy,Kidd and MNS.Blood grouping and extended phenotype tests were performed according to standard immunohematological procedures.Spot tests and tandem mass spectrometry were used respectively for the assessment of G6PD and sickle-cell anemia trait.RESULTS:The frequency of ABO phenotypes conformed to the following order O>A>B>AB with notably 62.5%,23.8%,12.1%and 1.6%for the Yansi,and 54.6%,27.5%,14.1%and 3.7%for the unrelated control group,respectively(P=0.19).As for the Rh phenotypes,the most frequent were cc D.ee,cc D.Ee,Cc D.ee,corresponding to 71.5%,12.1%and 12.1%for the Yansi,and 70.6%,15.6%and 8.2%,for the unrelated control group(P=0.27).The frequency of MN and Ss phenotypes were statistically different between groups(P=0.0021 and P=0.0006).G6PD was observed in 11.3%of subjects in the Yansi group,and in 12.4%of controls(P=0.74).The sickle-cell anemia trait was present in 22.4%of Yansi subjects and 17.8%in the control group(P=0.24).Miscarriages and deaths in young age were more common among Yansi people.CONCLUSION:This study shows a significant difference in MNS blood group distribution between the Yansi tribe and a control population.The distribution of other blood groups and the prevalence of hemoglobinopathies did not differ in the Yansi tribe.

全自动血型仪性能优化方案探索及献血者结果分析

目的 探索更科学、有效的血型检测设备性能验证方法,对使用年限较长、维修过的甲设备进行性能验证并优化。方法 甲、乙(已确认)两台设备同时进行4 026份献血员样本的平行比对实验,并以试管法作为补充对照实验方法。根据实验结果的符合率评价甲设备的性能,并适当调整甲设备ABO、Rh(D)血型检测的部分参数。通过效价测定确保甲设备抗原抗体检测的凝集强度与乙设备相当,并使用优化后的实验参数进行再次平行比对实验。结果 两种设备总符合率99.80%,Rh(D)血型符合率100%,ABO血型符合率99.98%,经血型研究室确认,共检出3例亚型:甲设备弱抗体、弱抗原漏检各1例,乙设备漏检弱抗原1例。脂血溶血样本甲设备未检出1例,乙设备未检出6例。甲设备、乙设备、试管法的抗-A、抗-B效价分别为8、32、128,抗-D效价分别为2、8、64。通过调整设备参数提高凝集效价。相关实验参数优化后,400份献血员标本比对总符合率为100%。结论 采供血机构关键设备维修后的性能验证是实验室质量管理体系的一部分。性能比对实验及多参数的优化提高了甲设备抗原/抗体的检测能力。

Correlation between movement of tectonicblocks and earthquakes in groups

The Chinese mainland is divided into some tectonic blocks by nearly NE- and EW-orientated faults. Meanwhile strong earthquakes in the Chinese mainland usually cluster in time and space. We call earthquakes in groups. Tectonic blocks separated by faults and earthquakes in groups are prominent features of the tectonics of the Chi-nese mainland. Correlation between movement of tectonic blocks and groups of earthquakes is discussed in this paper. The results show that earthquakes in groups often occurred at one or several block boundary faults. The released elastic strain energy is built up in the same periods and around blocks. It means that strong earthquakes in groups are mainly caused by movement of blocks. Four types of block movement are identified based ongroup earthquakes: movement along a single boundary of a block (or a combined blocks), movement of a single block, movement of multi-blocks, and movement in block interiors. If we consider distribution of all strong earthquakes occurred in the Chinese mainland, the movement along a single boundary of a block is more popular one inducing strong earthquakes. But if we only consider earthquakes in groups rather than single earthquakesthe movement of a block dominates among four modes. Statistics with respect to group earthquakes show that the Taihangshan mountain and the North China block are much active in the eastern part of Chinese mainland, and in western part of Chinese mainland the active blocks are Sichuan-Yunnan and the Kunlun-Songpan ones.

基于DRGs分组付费模式A型主动脉夹层手术住院费用影响因素分析

目的分析疾病诊断相关分组(DRGs)编码为A型主动脉夹层患者实际住院总花费的影响因素。方法回顾性分析2020年12月至2022年12月间DRGs付费模式实施前后河南省胸科医院心血管外科主动脉夹层手术患者的病案首页信息,统计分析DRGs付费模式实施前后患者住院费用结构变化及影响因素。结果DRGs实施后A型主动脉夹层手术患者与实施DRGs前相比,DRGs实施后总住院费用、手术药物相关费用、高价值手术耗材费用均显著降低,差异具备统计学意义(P<0.05)。医疗费用支出结构主要由高值耗材和术后费用(并发症处理费用,术后监护费用)构成,其中高值耗材支出占比最高。胸主动脉手术,不伴有极重度或严重的并发症和伴随症(FS14B组)、胸主动脉手术,伴有极重度或严重的并发症和伴随症(FS14A组)、胸主动脉介入手术(FS13Z组)分别为(98685.95±2990.18)元、(93427.57±4615.32)元、(85230.41±4483.12)元,占比分别为34.94%、33.73%、30.32%。A型主动脉夹层手术患者高倍率住院费用二元Logistic危险因素分析结果显示,住院天数≥7 d、重症监护室(ICU)干预频次≥1次、初始急性生理学和慢性健康状况评分(APACE II)(≥10分)、初始脑钠肽(BNP)(≥450 pg/mL)、心脏正电子发射断层扫描-计算机断层扫描(PET-CT)检查(是)、人工血管数量(≥2条)、使用血管支架(≥1支)、使用腔内修复装置、使用人工心脏瓣膜(≥1枚)、使用“达芬奇”手术机器人、年龄(≥65岁)是影响A型主动脉夹层手术住院费用的主要危险因素,差异具有统计学意义(P<0.05)。结论实施DRGs分组付费模式后主动脉夹层手术住院费用显著下降,可为主动脉夹层手术患者医保基金精细化管理及合理费用控制提供依据。

新文科视野下高校中文专业语言类新型课程群建设研究

新文科建设对高校中文专业语言类课程群的交叉融合和系统协同提出了更高的要求。在深入剖析语言类课程设置现状基础上,提出新型课程群的核心理念及导向,基于此构建课程群模式,即基础知识层级、交叉素养层级和实践能力层级,进而提出建设跨学科教师团队、转变教学方法和教学理念、策划语言实践活动等具体实现路径。整体思路以培养能力复合型人才为导向,明确了系统性的知识结构串联、课程耦合和协同机制、多学科交叉融合等理念,为新文科背景下新型课程群建设和落地实践提供了有力支撑。

教育、科技、人才一体推进的中国实践——“组团式”教育帮扶模式论析

“组团式”教育帮扶是教育帮扶模式的创新,对我国教育、科技、人才一体化推进具有重要的现实意义。研究G省8个重点帮扶县“组团式”教育帮扶的实践样态,发现其“组团式”教育帮扶主要涉及教育帮扶、科技帮扶、人才帮扶等实践帮扶机制,但在具体帮扶过程中仍存在持续性不强、力度不够、精准性不足等问题。基于此,根据协同理论,从教育帮扶主体、方式、过程、目标四个维度构建教育、科技、人才一体化推进的“组团式”教育帮扶模式。在后脱贫时代,高质量的“组团式”教育帮扶,还需加强科技帮扶力度,探索本土帮扶模式,促进教育、科技、人才帮扶一体化等,切实提高“组团式”教育帮扶成效。

消化性溃疡患者HMGB1、PINP和CD_(4)^(+)T细胞变化及与Hp感染的关系

目的探讨消化性溃疡患者高迁移率族蛋白B1(HMGB1)、I型原胶原N端前肽(PINP)和CD_(4)^(+)T细胞变化及与幽门螺杆菌(Hp)感染的关系。方法选择2019年9月—2021年9月我院收治的消化性溃疡患者97例作为研究对象,根据是否Hp感染分为Hp阳性组(n=63)与Hp阴性组(n=34);另选择同期我院健康体检者60例作为对照组。采用酶联免疫吸附法测定HMGB1和PINP水平;采用流式细胞仪测定CD_(4)^(+)T细胞水平。比较消化性溃疡组与对照组HMGB1、PINP和CD_(4)^(+)T细胞水平;不同Hp感染HMGB1、PINP和CD_(4)^(+)T细胞水平;采用Pearson分析Hp感染与HMGB1、PINP和CD_(4)^(+)T细胞相关性;采用多因素Logistic回归分析HMGB1、PINP和CD_(4)^(+)T细胞与Hp感染的关系。结果消化性溃疡组HMGB1水平高于对照组,PINP和CD_(4)^(+)T细胞水平低于对照组(P<0.05)。Hp阳性组HMGB1水平高于Hp阴性组,PINP和CD_(4)^(+)T细胞水平低于Hp阴性组(P<0.05)。经Pearson分析,HMGB1与Hp感染呈线性正相关,PINP和CD_(4)^(+)T细胞与Hp感染呈线性负相关(P<0.05)。经多因素Logistic回归分析HMGB1、PINP和CD_(4)^(+)T细胞为影响Hp感染危险因素。结论消化性溃疡患者HMGB1水平升高,PINP和CD_(4)^(+)T细胞水平下降,且与Hp感染密切相关,为影响Hp感染的危险因素。

高职航空类课程群课程思政教学系统化设计的策略及应用

课程思政教学是一项系统工程,需从专业群、课程群的角度进行顶层设计、系统推进。在实践的基础上分析了课程思政面临的新挑战,提出“三师协同、五步三入法”的课程群课程思政整体设计实践方法,以高职院校航空类课程群为例说明具体实施的策略及应用效果。

云南澜沧地区澜沧岩群变质岩石类型特征与原岩恢复

云南澜沧地区澜沧岩群出露于昌宁-孟连结合带西部,划分为曼来岩组片岩,原岩为泥岩、粉砂质泥质岩、泥质粉砂岩类;惠民岩组钠长千枚岩,原岩为流纹岩;南坑河岩组变质石英砂岩,原岩为石英砂岩。为一套含火山陆源碎屑沉积,其沉积环境为被动陆缘。澜沧岩群变质带经历了三期变形变质作用,早期为加里东期,主变质时期为华力西期,后期局部叠加印支期动力变质作用。

产生抗D的DVI type 3型孕妇免疫血清学和RHD基因型分析

目的:对部分D表型孕妇进行免疫血清学和RHD基因型分析。方法:采用常规血型血清学方法鉴定孕妇RhD血型,并进行血型特异性抗体筛查和鉴定;采用序列特异性引物聚合酶链反应(polymerase chain reactionsequence specific primer,PCR-SSP)鉴定孕妇RHD基因型;采用多重连接依赖的探针扩增技术(multiplex ligationdependent probe amplification,MLPA)对孕妇及其配偶和女儿的RhD血型抗原进行基因分型及遗传分析。结果:该孕妇血清中检测出IgG抗-D,其抗体效价为1∶8。PCR-SSP结果显示,该孕妇RHD基因第3-6外显子缺失,经鉴定该孕妇RHD基因型为DVI type 3型。MLPA分析显示,该孕妇只有1条RHD等位基因,且缺失3-6外显子,其基因型为CDVIe/cde,其配偶为CDe/CDe纯合子基因型,女儿为CDe/CDVIe基因型。结论:准确的RhD血型鉴定对制定安全有效的临床输血策略和对育龄妇女采取恰当措施及预防新生儿溶血病具有重要意义。

GPRC5A调控的ABCB1表达对肺腺癌增殖的影响

目的ATP结合盒B亚家族成员1(ATP binding cassette subfamily B member 1,ABCB1)的异常表达在多种癌症的发生发展中发挥关键作用。然而,G蛋白偶联受体C家族5组A型(G protein coupled receptor family C group5 type A,GPRC5A)调控的ABCB1表达对肺腺癌增殖的影响仍不清楚。本研究探讨了GPRC5A调控的ABCB1表达对肺腺癌增殖的影响。方法我们采用RT-PCR、Western-blot或免疫组化实验,分析ABCB1在肺腺癌细胞系、人肺腺癌组织以及GPRC5A基因敲除小鼠和野生型小鼠的气管上皮细胞和肺组织中的表达。采用细胞计数试剂盒-8(CCK-8)分析GPRC5A基因敲除小鼠气管上皮细胞对化疗药物的敏感性。采用皮下肿瘤形成实验探讨下调ABCB1表达是否可抑制体内肺腺癌增殖。采用免疫荧光和免疫沉淀实验研究GPRC5A和ABCB1之间潜在的调控关系。结果ABCB1在肺腺癌细胞系和人类肺腺癌组织中表达上调。GPRC5A基因敲除小鼠的气管上皮细胞及肺组织的ABCB1表达高于野生型小鼠。与GPRC5A野生型小鼠的气管上皮细胞相比,GPRC5A基因敲除小鼠的气管上皮细胞对塔立奇达和多柔比星更敏感。注射移植细胞28天后,接受ABCB1基因敲除细胞移植的GPRC5A-/-C57BL/6小鼠的肺肿瘤的体积和重量均明显低于野生型细胞移植小鼠(P=0.0043,P=0.0060)。此外,免疫荧光和免疫沉淀实验表明,GPRC5A通过直接结合方式调控ABCB1的表达。结论GPRC5A通过抑制ABCB1表达降低肺腺癌增殖。GPRC5A调节ABCB1表达的途径有待研究。

2型固有淋巴细胞在急性肾损伤中的作用研究进展

急性肾损伤(AKI)是全球的主要公共健康问题之一,其发病原因复杂,死亡率高。其中,免疫细胞及其分泌的炎症因子是诱导AKI发病的关键因素。2型固有淋巴细胞(ILC2)是参与2型免疫的固有淋巴细胞,在AKI中具有潜在的治疗价值,其在肾缺血再灌注损伤、顺铂或阿霉素诱导的AKI动物模型中发挥重要保护作用。白细胞介素-33是介导肾脏中ILC2扩增的关键细胞因子。然而,其作为一种警报素在自身免疫性疾病中也发挥潜在的致病作用。未来需对ILC2的生物学功能及其在AKI中发挥的保护作用机制进行更深入的研究,以期为AKI的治疗提供新靶点。

蒽醌型染料分子羟基最优取代位置的多目标优化设计方法

为解决染料分子设计中染料溶解度与色谱深度2个指标的矛盾问题,首先将染料溶解度与色谱深度2个目标优化问题转化成以染料分子溶解度为优化目标函数,色谱深度函数为约束,羟基取代位置为优化变量的单目标0-1规划问题。其中染料溶解度用内聚能表示,建立了内聚能与羟基取代位置的模型;染料色谱深度用可见光波段的最大吸收光谱强度表示,建立了最大吸收光谱强度与分子母体上的羟基数和羟基取代位置的模型。然后根据蒽醌型染料分子结构的几何特征,以分子对称性为一层启发规则,排除重复取代位置,减少可行路径;通过隐数法迭代计算出在色谱深度不低于设定值条件下,内聚能减小最多的羟基最优取代位置。最后以一种蒽醌型染料母体为实例,进行不同羟基数和羟基最优取代位置及数量溶解过程模拟实验,将其溶解度与实际相似结构染料进行对比,结果表明本文分子结构优化设计方法具有有效性。