Objective:To evaluate vascular endothelial growth factor(VEGF)levels in hepatocellular carcinoma patients before and after transcatheter arterial chemoembolization(TACE)and percutaneous ethanol injection(PEI)and its r...Objective:To evaluate vascular endothelial growth factor(VEGF)levels in hepatocellular carcinoma patients before and after transcatheter arterial chemoembolization(TACE)and percutaneous ethanol injection(PEI)and its relation to treatment response.Methods:A total of 40 patients with unrespectable hepatocelluar carcinoma were assessed clinically.Twenty patients were suitable to be treated by TACE,while other 20patients were treated with PEI.Serum VEGF levels were measured before and 1 month after each procedure by ELISA.Response was assessed after 1 month according to Union Internationale Contre le Cancer evaluation criteria based on change in tumor size as measured by ultrasound.Results:There was no significant difference between TACE and PEI groups with regard to age,sex,tumor size,response to local therapy,or VEGF and alpha-fetoprotein before and after therapy.VEGF levels after TACE were significantly higher than before TACE[(298.1±123.6)pg/m L vs.(205.8±307.3)pg/m L;P=0.001].Also,VEGF levels were significantly higher after PEI than before PEI[(333.8±365.6)pg/m L vs.(245.3±301.8)pg/m L;P=0.000].Non-responders of both groups had significantly high VEGF levels than responder's,both before[(985.0±113.2)pg/m L vs.(117.1±75.3)pg/m L;P<0.001]and after therapy[(1 330.6±495.7)pg/m L vs.(171.0±94.7)pg/m L;P=0.000)].Conclusions:Both TACE and PEI were associated with an increase in serum VEGF in hepatocelluar carcinoma patients.Higher levels of VEGF before and after therapy were found in non-responders,suggesting that VEGF is a useful marker in predicting treatment response.展开更多
Human endothelial cells derived from umbilical vein could besubcultured and survive 25 passage (double time 20～25 hours, 60～75 cumula-tive population doublings). An observation was performed with light mi-croscopy, ...Human endothelial cells derived from umbilical vein could besubcultured and survive 25 passage (double time 20～25 hours, 60～75 cumula-tive population doublings). An observation was performed with light mi-croscopy, electron microscopy and immunofluorescence microscopy using specificantiserum against factor Ⅷrelated antigen. It identified that the cultured cellswere endothelial cells. Medium RPMI-1640 supplemented with 20% humanserum, endothelial cell growth factor 200μg/ml, heparin 100μg/ml and gelatincoated flasks were very important conditions for long-term culture of humanendothelial cell.展开更多
Modern subsoiling technology is a little ploughing measure in dry land protective farming technology system.The subsoiler can scarify soil,deepen the arable layer,break the plough layer,improve the soil permeability,i...Modern subsoiling technology is a little ploughing measure in dry land protective farming technology system.The subsoiler can scarify soil,deepen the arable layer,break the plough layer,improve the soil permeability,increase soil infiltration speed and amount,and create an arable layer structure with coexistence of fictional and real situation.Also,it can effectively promote development and growth of crop root system and play a favorable role in drought resistance and yield increase of crops.This paper made a simple overview of the subsoiling technology,current development situation of subsoiler both at home and abroad,and classification of subsoilers.展开更多
Anodization is a popular method of preparing TiO2nanotube array films（TiNTs） by using direct current（DC）power as the driving voltage.In this study,three driving voltage modes,namely,the sine alternating current（s...Anodization is a popular method of preparing TiO2nanotube array films（TiNTs） by using direct current（DC）power as the driving voltage.In this study,three driving voltage modes,namely,the sine alternating current（sine） mode,the full-wave rectification of sine waves via four diodes（sine-4D,where D means diode） mode,and the DC mode,were used to prepare TiNTs by anodization.At 20 V,TiNTs were formed under sine-4D mode but only irregular porous TiO2films were formed under DC mode.At 50 V,TiNTs formed under both the sine-4D and DC modes.No TiNTs formed in the sine mode anodization at either 20 or 50 V.Compared with the DC mode,the sine-4D mode required a lower oxidation voltage for TiNT formation,which suggests that sine-4D is an economical,convenient,and efficient driving voltage for TiNT preparation by anodization.The morphologies and structures of TiNT samples anodized at 50 V in the sine-4D and DC modes at different oxidation time（1,5,10,30,60,and 120 min） were analyzed.TiNT growth processes were similar between the studied modes.However,the growth rate of the films was faster under the sine-4D mode than the DC mode during the first 30 min of anodization.展开更多
文摘Objective:To evaluate vascular endothelial growth factor(VEGF)levels in hepatocellular carcinoma patients before and after transcatheter arterial chemoembolization(TACE)and percutaneous ethanol injection(PEI)and its relation to treatment response.Methods:A total of 40 patients with unrespectable hepatocelluar carcinoma were assessed clinically.Twenty patients were suitable to be treated by TACE,while other 20patients were treated with PEI.Serum VEGF levels were measured before and 1 month after each procedure by ELISA.Response was assessed after 1 month according to Union Internationale Contre le Cancer evaluation criteria based on change in tumor size as measured by ultrasound.Results:There was no significant difference between TACE and PEI groups with regard to age,sex,tumor size,response to local therapy,or VEGF and alpha-fetoprotein before and after therapy.VEGF levels after TACE were significantly higher than before TACE[(298.1±123.6)pg/m L vs.(205.8±307.3)pg/m L;P=0.001].Also,VEGF levels were significantly higher after PEI than before PEI[(333.8±365.6)pg/m L vs.(245.3±301.8)pg/m L;P=0.000].Non-responders of both groups had significantly high VEGF levels than responder's,both before[(985.0±113.2)pg/m L vs.(117.1±75.3)pg/m L;P<0.001]and after therapy[(1 330.6±495.7)pg/m L vs.(171.0±94.7)pg/m L;P=0.000)].Conclusions:Both TACE and PEI were associated with an increase in serum VEGF in hepatocelluar carcinoma patients.Higher levels of VEGF before and after therapy were found in non-responders,suggesting that VEGF is a useful marker in predicting treatment response.
基金Project Supported By the National Natural Science Fund
文摘Human endothelial cells derived from umbilical vein could besubcultured and survive 25 passage (double time 20～25 hours, 60～75 cumula-tive population doublings). An observation was performed with light mi-croscopy, electron microscopy and immunofluorescence microscopy using specificantiserum against factor Ⅷrelated antigen. It identified that the cultured cellswere endothelial cells. Medium RPMI-1640 supplemented with 20% humanserum, endothelial cell growth factor 200μg/ml, heparin 100μg/ml and gelatincoated flasks were very important conditions for long-term culture of humanendothelial cell.
基金Supported by Project of National Natural Science Foundation(51105123)Key Science and Technology Program of Hainan Province(ZDXM20120074)
文摘Modern subsoiling technology is a little ploughing measure in dry land protective farming technology system.The subsoiler can scarify soil,deepen the arable layer,break the plough layer,improve the soil permeability,increase soil infiltration speed and amount,and create an arable layer structure with coexistence of fictional and real situation.Also,it can effectively promote development and growth of crop root system and play a favorable role in drought resistance and yield increase of crops.This paper made a simple overview of the subsoiling technology,current development situation of subsoiler both at home and abroad,and classification of subsoilers.
基金the financial support provided by the National Natural Science Foundation of China (Nos. 21277004, 21190051 and 41121004)the Beijing Natural Science Foundation, China (No. 8132035)
文摘Anodization is a popular method of preparing TiO2nanotube array films（TiNTs） by using direct current（DC）power as the driving voltage.In this study,three driving voltage modes,namely,the sine alternating current（sine） mode,the full-wave rectification of sine waves via four diodes（sine-4D,where D means diode） mode,and the DC mode,were used to prepare TiNTs by anodization.At 20 V,TiNTs were formed under sine-4D mode but only irregular porous TiO2films were formed under DC mode.At 50 V,TiNTs formed under both the sine-4D and DC modes.No TiNTs formed in the sine mode anodization at either 20 or 50 V.Compared with the DC mode,the sine-4D mode required a lower oxidation voltage for TiNT formation,which suggests that sine-4D is an economical,convenient,and efficient driving voltage for TiNT preparation by anodization.The morphologies and structures of TiNT samples anodized at 50 V in the sine-4D and DC modes at different oxidation time（1,5,10,30,60,and 120 min） were analyzed.TiNT growth processes were similar between the studied modes.However,the growth rate of the films was faster under the sine-4D mode than the DC mode during the first 30 min of anodization.
