AIM:To detect the impact of insulin-like growth factor-1(IGF-1)and other risk factors for the early prediction of retinopathy of prematurity(ROP)and to establish a scoring system for ROP prediction by using clini...AIM:To detect the impact of insulin-like growth factor-1(IGF-1)and other risk factors for the early prediction of retinopathy of prematurity(ROP)and to establish a scoring system for ROP prediction by using clinical criteria and serum IGF-1 levels.METHODS:The study was conducted with 127 preterm infants.IGF-1 levels in the 1st day of life,1st,2nd,3rd and4th week of life was analyzed.The score was established after logistic regression analysis,considering the impact of each variable on the occurrences of any stage ROP.A validation cohort containing 107 preterm infants was included in the study and the predictive ability of ROP score was calculated.RESULTS:Birth weights(BW),gestational weeks(GW)and the prevalence of breast milk consumption were lower,respiratory distress syndrome(RDS),bronchopulmonarydysplasia(BPD)and necrotizing enterocolitis(NEC)were more frequent,the duration of mechanical ventilation and oxygen supplementation was longer in patients with ROP(P〈0.05).Initial serum IGF-1 levels tended to be lower in newborns who developed ROP.Logistic regression analysis revealed that low BW(〈1250 g),presence of intraventricular hemorrhage(IVH)and formula feeding increased the risk of ROP.Afterwards,the scoring system was validated on 107 infants.The negative predictive values of a score less than 4 were 84.3%,74.7%and 79.8%while positive predictive values were 76.3%,65.5%and71.6%respectively.CONCLUSION:In addition to BW〈1250 g and IVH,formula consumption was detected as a risk factor for the development of ROP.Breastfeeding is important for prevention of ROP in preterm infants.展开更多
We evaluated the effect of ration level(RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok(initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: sta...We evaluated the effect of ration level(RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok(initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: starvation,2%,3%,4% bwd(body weight per day,based on initial mean values),and apparent satiation. Feed consumption,apparent digestibility,and growth were directly measured. Specific growth rates in terms of wet weight,dry weight,protein,and energy increased logarithmically with an increase in ration levels. The relationship between specific growth rate in terms of wet weight(SGRw,%/d) and RL(%) was characterized by a decelerating curve: SGRw=-1.417+3.166ln(RL+1). The apparent digestibility coefficients of energy exhibited a decreasing pattern with increasing ration level,and there was a significant difference among different RLs. Body composition was significantly affected by ration size. The relationship between feed efficiency rate in terms of energy(FERe) and RL was: FERe=-14.167+23.793RL–3.367(RL)2,and the maximum FERe was observed at a 3.53% ration. The maintenance requirement for energy of juvenile lenok was 105.39 k J BW(kg)-0.80/d,the utilization efficiency of DE for growth was 0.496. The energy budget equation at satiation was: 100IE=29.03FE+5.78(ZE+UE)+39.56 HE+25.63 RE,where IE is feed energy,FE is fecal energy,ZE+UE is excretory energy,HE is heat production,and RE is recovered energy. Our results suggest that the most suitable feeding rate for juvenile lenok aquaculture for wet weight growth is 2.89% bwd,whereas for energy growth,the suggested rate is 3.53% bwd at this growth stage.展开更多
The purpose of the study is to measure the "growth drag" according to the characteristic of Chinese land resource.Romer model (2001) holds that every country is inevitably affected by the "growth drag&q...The purpose of the study is to measure the "growth drag" according to the characteristic of Chinese land resource.Romer model (2001) holds that every country is inevitably affected by the "growth drag" due to the limitation of land resource.So it's of profound strategic significance to measure the "growth drag" according to the character of Chinese land resource.Modified two-level CES production function was employed,and this paper modified the hypothesis of the model.The result indicates that the limitation of Chinese land resource casts shadow over the economic development of China,and the growth rate is 0.4618% lower than that without the limitation of land resource.Through implementing the land resource protection policy along with the technology improvement and the substitute effect of other factors to the land resource,China will keep a steady and balanced economic growth.展开更多
A 45-day experiment was carried out to evaluate the growth performance, digestive enzymes activity and biochemical response of Cirrhinus mrigala fed graded levels of oil. Three isonitrogenous purified diets (6%, 8% ...A 45-day experiment was carried out to evaluate the growth performance, digestive enzymes activity and biochemical response of Cirrhinus mrigala fed graded levels of oil. Three isonitrogenous purified diets (6%, 8% and 10% of oil) were formulated. A total of 108 juveniles of Cirrhinus mrigala (5.95±0.25) g were equally distributed in triplicate groups in nine plastic tanks of 100 L capacity and fed under natural light cycle. Growth performance of the fish significantly reflected the effect of inclusion levels of oil tested but the diet with 8% lipid had the best significant growth performance [% weight gain, Feed Conversion Ratio (FCR) and Specific Growth Rate (SGR)], across different experimental groups. Levels of secretion of digestive enzymes in the fish fed different experimental diets resulted in significant values of (p〈0.05) amylase and lipase, while Aspartate transaminase (AST), Alanine transaminase (ALT), Lactate dehydrogenase (LDH) and Malate dehydrognnase (MDH) values in the muscle and liver decreased significantly (F〈0.05) as the levels of oii in the diet increased. However, serum biochemical parameters did not record any significant difference 6o〉0.05) with the exception of the total lipid across diets. The study confirmed the inclusion effect of graded levels ofoil in juvenile Mrigal diet, but the inclusion should not be more than 8% to avert growth depression.展开更多
BACKGROUND: Insulin-like growth factor-I(IGF-1), as one of the important members of growth factor family, participants in the regulation of many physiological functions and behaviors, having very strong neuroprotec...BACKGROUND: Insulin-like growth factor-I(IGF-1), as one of the important members of growth factor family, participants in the regulation of many physiological functions and behaviors, having very strong neuroprotective effect. However, the expression of IGF-1 following cerebral ischemia/reperfusion is still disputed. OBJECTIVE: To observe the expression of IGF-1 and protein of corpora striata in ischemic side at the early stage of middle cerebral artery ischemia/reperfusion in rhesus monkey. DESIGN : A completely randomized grouping design, controlled animal experiment SETTING : Institute of Cerebrovascular Disease, Affiliated Hospital of Medical College of Qingdao University MATERIALS: ① Totally 17 rhesus monkeys , of either gender, aged 4 to 5 years, were enrolled . Seven rhesus monkeys observed with gene chip were randomly divided into 2 groups: sham operation group (n=3) and ischemia/reperfusion group 〈n=4〉. Ten rhesus monkeys observed with in situ hybridization and immunohistochemistry method were randomly divided into 2 groups: sham operation group 〈n=3 〉and ischemia/reperfusion group (n=7). Rhesus monkeys observed under microscope were divided into 2 groups: sham operation group (n=6) and ischamia/reperfusion group (n=-11).②Materials used in the experiment: cresyl violet (Sigma Company, America); immunohistochemical reagent kit ( Huamei Bio-engineering Company); In situ hybridization reagent kit (Boshide Bio-engineering Co.Ltd, Wuhan); 12 800 dots chip (Boxing Company, Shanghai). METHODS : This experiment was carried out at the Institute of Cerebrovascular Disease, Affiliated Hospital of Medical College of Qingdao University from January 2001 to December 2003.① The onset area of middle cerebral artery was blocked for 2 hours, middle cerebral artery ischemia/reperfusion models were created.② After ischemia/reperfusion for 24 hours, cerebral tissue sections of rhesus monkeys were prepared and stained with cresyl violet. Image analysis was performed with 5001W image analysis software. Morphological change of corpora striata of operative side was observed in the rhesus monkeys between two groups. Total RNA was extracted from cerebral tissue. ③ Detection of gene chip: Cy3-duTP and Cy5-duTP were used to respectively perform reverse transcription labeling. The sample was reversely transcribed into cDNA, then hybridized with cDNA of cerebral tissue. Genes with the separate absolute value of cy3 and cy5〉800, cY3/cy5 〉 2(high expression) or 〈 0.5 (low expression) were found out. Those were genes with differential expression. ④ The expressions of IGF-1 mRNA and protein level of corpora striata in ischemic side of rhe- sus monkeys were detected between sham operation group and ischemia/reperfusion group at 9 and 24 hours after ischemia/reperfusion with in situ hybridization method and immunohistochemical method. Brown granules were IGF-1 protein positive cells. ⑤ Analysis of variance was used in the difference comparison of measurement data among groups. MAIN OUTCOME MEASURES : ① Change of morphological structure of corpora striata at ischemic side in rhesus monkeys. ② Change of cerebral gene expression profiles at ischemia/reperfusion in rhesus monkeys between two groups.③ Expression of IGF-1 mRNA and protein level of corpora striata at ischemia/reperfu- sion in rhesus monkeys between two groups. RESULTS : ① Pathological change : Obvious pathological change of cerebral infarction appeared in the ischemia and reperfusion group, while there was no such pathological change in the sham operation group.② Change of gene expression profile : There were 4480 genes with difference expression in the ischemia/reperfusion group and sham-operation group, in which, 260 genes had high expression and their absolute value was over 800, and 63 genes had low expression, cy3/cy5 of IGF-1 was 0.379, being relative low ex- pression. ③ IGF-1 mRNA and protein positive cell counts in corpora striata at cerebral ischemic side[IGF-1 mRNA: 〈9.72±1.18),(9.11 ±0.76),(14.77±0.60) counts/field:lGF-1 protein: (15.11 ±1.83),(15.39±0.78), (34.62±0.97)counts/field, P 〈 0.05-0.01]. CONCLUSION: IGF-1 mRNA and protein are lowly expressed in middle cerebral artery of rhesus monkeys at ischemia/reperfusion.展开更多
[ Objective] The research aimed to study influence of the repeated flooding on growth and development of A. calamus in water-level-fluc- tuation zone of the Three Gorges Reservoir. [ Method] A. calamus plants were exp...[ Objective] The research aimed to study influence of the repeated flooding on growth and development of A. calamus in water-level-fluc- tuation zone of the Three Gorges Reservoir. [ Method] A. calamus plants were exposed to water under the dark conditions respectively in Septem- ber 2009 and September 2010. Then, they were taken away from the water, and grew in natural conditions in the following March and April respec- tively ( marked as S1, S2, S1 and S2). The plant number was conducted statistics respectively. On May 7, the leaf number was made statistics. Length, width and rapid light responding curve of the leaf were determined. [ Result] Repeated flooding restrained significantly plant germination un- der the dark condition. Plant number in S1 and S2 groups decreased by 38.9% and 33.3% respectively compared with the control. It also reduced survival rate of the plant. Plant number in S2 group decreased by 16.7% (P 〈 0.05) compared with that of S2 group when they were taken away from the water. Both of flooding promoted elongation of the leaves, restrained leaves to grow wider, and also restrained leaf formation of the plants except for S1 and S1 groups. Total leaf length of a plant decreased markedly after the second flooding which in S1 and S2 groups were 50.6% and 36.9% respectively less than that after the first flooding. Total leaf number of the plant in S1and S2 groups reduced significantly, and they were only 63.1% and 38.5% respectively of the control. Compared with the first flooding, total leaf length of a plant in the control increased signifi- cantly and decreased remarkably in S2 group after the second flooding. Furthermore, light response ability of the relative electronic transfer rate (rETR) in S1 group wasn't significantly different from the control, and rETRmax, in S2 group was significantly less than the control. Moreover, non- photochemical quenching (NPQ) decreased remarkably in S1 and S2 groups. It indicated that A. calamus had good restore ability of the light re- sponse, but restoring of its heat dissipation capacity was slower. [ Conclusion] Repeated flooding inhibited plant growth and population recovery of A. calamus under the dark condition.展开更多
To study the effect of IL-18 and nitric oxide(NO) on the growth and metastasis of non-small cell lung cancer (NSCLC).Methods Serum IL-18 and nitrate and nitrite levels in 82 patients with NSCLC and 20 healthy control ...To study the effect of IL-18 and nitric oxide(NO) on the growth and metastasis of non-small cell lung cancer (NSCLC).Methods Serum IL-18 and nitrate and nitrite levels in 82 patients with NSCLC and 20 healthy control subjects were measured by using ELISA and Griess.Results The levels of serum IL-18 were (334.2±31.0)ng/L in NSCLC patients and (151.3±22.0)ng/L in control subjects,respectively.The levels of nitrate and nitrite were (237.1±21.0)μmol/L in NSCLC patients and (44.2±15.0)μmol/L in control subjects.The levels of serum IL-18 and nitrate and nitrite were not related with age,gender,histological types in patients with NSCLC.The levels of serum IL-18 was closely associated with TNM stage,lymph node metastasis and distal metastasis,but not with its degree and organ types of metastasis.There was a negative correlation between the levels of serum IL-18 and nitrate and nitrite.Conclusion Serum IL-18 and nitrate and nitrite levels may be useful to evaluate the prognosis of the patients with NSCLC.16 refs,2 tabs.展开更多
试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组...试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组分别饲喂粗蛋白质水平为13.43%、15.04%、16.64%和18.25%的饲粮。试验期46 d,其中孵化期18 d (1~18 d),哺乳期28 d (19~46 d)。结果显示,试验Ⅱ组、Ⅲ组产蛋间隔显著低于试验Ⅰ组(P<0.05),试验Ⅲ组种蛋受精率显著高于试验Ⅰ组、Ⅳ组(P<0.05),试验Ⅲ组入孵蛋孵化率极显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.01)。试验Ⅲ组乳鸽28日龄体重显著高于试验Ⅰ组(P<0.05),乳鸽屠宰率极显著高于试验Ⅰ组(P<0.01),乳鸽胸肌率显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.05)。试验Ⅱ组、Ⅲ组、Ⅳ组窝料重比1极显著低于试验Ⅰ组(P<0.01)。研究表明,不同粗蛋白质水平影响了种鸽的繁殖性能、乳鸽的生长性能以及窝料重比,在“2+2”生产模式下,建议白王种鸽饲粮适宜粗蛋白质水平为16.64%。展开更多
文摘AIM:To detect the impact of insulin-like growth factor-1(IGF-1)and other risk factors for the early prediction of retinopathy of prematurity(ROP)and to establish a scoring system for ROP prediction by using clinical criteria and serum IGF-1 levels.METHODS:The study was conducted with 127 preterm infants.IGF-1 levels in the 1st day of life,1st,2nd,3rd and4th week of life was analyzed.The score was established after logistic regression analysis,considering the impact of each variable on the occurrences of any stage ROP.A validation cohort containing 107 preterm infants was included in the study and the predictive ability of ROP score was calculated.RESULTS:Birth weights(BW),gestational weeks(GW)and the prevalence of breast milk consumption were lower,respiratory distress syndrome(RDS),bronchopulmonarydysplasia(BPD)and necrotizing enterocolitis(NEC)were more frequent,the duration of mechanical ventilation and oxygen supplementation was longer in patients with ROP(P〈0.05).Initial serum IGF-1 levels tended to be lower in newborns who developed ROP.Logistic regression analysis revealed that low BW(〈1250 g),presence of intraventricular hemorrhage(IVH)and formula feeding increased the risk of ROP.Afterwards,the scoring system was validated on 107 infants.The negative predictive values of a score less than 4 were 84.3%,74.7%and 79.8%while positive predictive values were 76.3%,65.5%and71.6%respectively.CONCLUSION:In addition to BW〈1250 g and IVH,formula consumption was detected as a risk factor for the development of ROP.Breastfeeding is important for prevention of ROP in preterm infants.
基金Supported by the Special Fund for Agro-Scientific Research in the Public Interest of China(No.201003055)the National Key Technology Research and Development Program of China(Nos.2012BAD25B10,2012BAD26B05)the Central-Level Non-Profit Scientific Research Institutes Special Funds of China(No.HSY201412)
文摘We evaluated the effect of ration level(RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok(initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: starvation,2%,3%,4% bwd(body weight per day,based on initial mean values),and apparent satiation. Feed consumption,apparent digestibility,and growth were directly measured. Specific growth rates in terms of wet weight,dry weight,protein,and energy increased logarithmically with an increase in ration levels. The relationship between specific growth rate in terms of wet weight(SGRw,%/d) and RL(%) was characterized by a decelerating curve: SGRw=-1.417+3.166ln(RL+1). The apparent digestibility coefficients of energy exhibited a decreasing pattern with increasing ration level,and there was a significant difference among different RLs. Body composition was significantly affected by ration size. The relationship between feed efficiency rate in terms of energy(FERe) and RL was: FERe=-14.167+23.793RL–3.367(RL)2,and the maximum FERe was observed at a 3.53% ration. The maintenance requirement for energy of juvenile lenok was 105.39 k J BW(kg)-0.80/d,the utilization efficiency of DE for growth was 0.496. The energy budget equation at satiation was: 100IE=29.03FE+5.78(ZE+UE)+39.56 HE+25.63 RE,where IE is feed energy,FE is fecal energy,ZE+UE is excretory energy,HE is heat production,and RE is recovered energy. Our results suggest that the most suitable feeding rate for juvenile lenok aquaculture for wet weight growth is 2.89% bwd,whereas for energy growth,the suggested rate is 3.53% bwd at this growth stage.
基金founded by the Excellent Young Teachers Program of Shanghai Municipal Education Commission (Grant No. B8101090039)the Project for Doctoral Research Fund of Shanghai Ocean University (Grant No.A2400080314)
文摘The purpose of the study is to measure the "growth drag" according to the characteristic of Chinese land resource.Romer model (2001) holds that every country is inevitably affected by the "growth drag" due to the limitation of land resource.So it's of profound strategic significance to measure the "growth drag" according to the character of Chinese land resource.Modified two-level CES production function was employed,and this paper modified the hypothesis of the model.The result indicates that the limitation of Chinese land resource casts shadow over the economic development of China,and the growth rate is 0.4618% lower than that without the limitation of land resource.Through implementing the land resource protection policy along with the technology improvement and the substitute effect of other factors to the land resource,China will keep a steady and balanced economic growth.
文摘A 45-day experiment was carried out to evaluate the growth performance, digestive enzymes activity and biochemical response of Cirrhinus mrigala fed graded levels of oil. Three isonitrogenous purified diets (6%, 8% and 10% of oil) were formulated. A total of 108 juveniles of Cirrhinus mrigala (5.95±0.25) g were equally distributed in triplicate groups in nine plastic tanks of 100 L capacity and fed under natural light cycle. Growth performance of the fish significantly reflected the effect of inclusion levels of oil tested but the diet with 8% lipid had the best significant growth performance [% weight gain, Feed Conversion Ratio (FCR) and Specific Growth Rate (SGR)], across different experimental groups. Levels of secretion of digestive enzymes in the fish fed different experimental diets resulted in significant values of (p〈0.05) amylase and lipase, while Aspartate transaminase (AST), Alanine transaminase (ALT), Lactate dehydrogenase (LDH) and Malate dehydrognnase (MDH) values in the muscle and liver decreased significantly (F〈0.05) as the levels of oii in the diet increased. However, serum biochemical parameters did not record any significant difference 6o〉0.05) with the exception of the total lipid across diets. The study confirmed the inclusion effect of graded levels ofoil in juvenile Mrigal diet, but the inclusion should not be more than 8% to avert growth depression.
基金the Natural Science Foundation of Shandong Province, No. Y2004C04
文摘BACKGROUND: Insulin-like growth factor-I(IGF-1), as one of the important members of growth factor family, participants in the regulation of many physiological functions and behaviors, having very strong neuroprotective effect. However, the expression of IGF-1 following cerebral ischemia/reperfusion is still disputed. OBJECTIVE: To observe the expression of IGF-1 and protein of corpora striata in ischemic side at the early stage of middle cerebral artery ischemia/reperfusion in rhesus monkey. DESIGN : A completely randomized grouping design, controlled animal experiment SETTING : Institute of Cerebrovascular Disease, Affiliated Hospital of Medical College of Qingdao University MATERIALS: ① Totally 17 rhesus monkeys , of either gender, aged 4 to 5 years, were enrolled . Seven rhesus monkeys observed with gene chip were randomly divided into 2 groups: sham operation group (n=3) and ischemia/reperfusion group 〈n=4〉. Ten rhesus monkeys observed with in situ hybridization and immunohistochemistry method were randomly divided into 2 groups: sham operation group 〈n=3 〉and ischemia/reperfusion group (n=7). Rhesus monkeys observed under microscope were divided into 2 groups: sham operation group (n=6) and ischamia/reperfusion group (n=-11).②Materials used in the experiment: cresyl violet (Sigma Company, America); immunohistochemical reagent kit ( Huamei Bio-engineering Company); In situ hybridization reagent kit (Boshide Bio-engineering Co.Ltd, Wuhan); 12 800 dots chip (Boxing Company, Shanghai). METHODS : This experiment was carried out at the Institute of Cerebrovascular Disease, Affiliated Hospital of Medical College of Qingdao University from January 2001 to December 2003.① The onset area of middle cerebral artery was blocked for 2 hours, middle cerebral artery ischemia/reperfusion models were created.② After ischemia/reperfusion for 24 hours, cerebral tissue sections of rhesus monkeys were prepared and stained with cresyl violet. Image analysis was performed with 5001W image analysis software. Morphological change of corpora striata of operative side was observed in the rhesus monkeys between two groups. Total RNA was extracted from cerebral tissue. ③ Detection of gene chip: Cy3-duTP and Cy5-duTP were used to respectively perform reverse transcription labeling. The sample was reversely transcribed into cDNA, then hybridized with cDNA of cerebral tissue. Genes with the separate absolute value of cy3 and cy5〉800, cY3/cy5 〉 2(high expression) or 〈 0.5 (low expression) were found out. Those were genes with differential expression. ④ The expressions of IGF-1 mRNA and protein level of corpora striata in ischemic side of rhe- sus monkeys were detected between sham operation group and ischemia/reperfusion group at 9 and 24 hours after ischemia/reperfusion with in situ hybridization method and immunohistochemical method. Brown granules were IGF-1 protein positive cells. ⑤ Analysis of variance was used in the difference comparison of measurement data among groups. MAIN OUTCOME MEASURES : ① Change of morphological structure of corpora striata at ischemic side in rhesus monkeys. ② Change of cerebral gene expression profiles at ischemia/reperfusion in rhesus monkeys between two groups.③ Expression of IGF-1 mRNA and protein level of corpora striata at ischemia/reperfu- sion in rhesus monkeys between two groups. RESULTS : ① Pathological change : Obvious pathological change of cerebral infarction appeared in the ischemia and reperfusion group, while there was no such pathological change in the sham operation group.② Change of gene expression profile : There were 4480 genes with difference expression in the ischemia/reperfusion group and sham-operation group, in which, 260 genes had high expression and their absolute value was over 800, and 63 genes had low expression, cy3/cy5 of IGF-1 was 0.379, being relative low ex- pression. ③ IGF-1 mRNA and protein positive cell counts in corpora striata at cerebral ischemic side[IGF-1 mRNA: 〈9.72±1.18),(9.11 ±0.76),(14.77±0.60) counts/field:lGF-1 protein: (15.11 ±1.83),(15.39±0.78), (34.62±0.97)counts/field, P 〈 0.05-0.01]. CONCLUSION: IGF-1 mRNA and protein are lowly expressed in middle cerebral artery of rhesus monkeys at ischemia/reperfusion.
基金Supported by Natural Science Foundation Project,Chongqing Science and Technology Commission(CSTC) ,China(2009BB7255)Talent Start-up Foundation Project ,Chongqing University of Arts and Science,ChinaScience Research Project,Chongqing University of Arts and Science(XZ031)
文摘[ Objective] The research aimed to study influence of the repeated flooding on growth and development of A. calamus in water-level-fluc- tuation zone of the Three Gorges Reservoir. [ Method] A. calamus plants were exposed to water under the dark conditions respectively in Septem- ber 2009 and September 2010. Then, they were taken away from the water, and grew in natural conditions in the following March and April respec- tively ( marked as S1, S2, S1 and S2). The plant number was conducted statistics respectively. On May 7, the leaf number was made statistics. Length, width and rapid light responding curve of the leaf were determined. [ Result] Repeated flooding restrained significantly plant germination un- der the dark condition. Plant number in S1 and S2 groups decreased by 38.9% and 33.3% respectively compared with the control. It also reduced survival rate of the plant. Plant number in S2 group decreased by 16.7% (P 〈 0.05) compared with that of S2 group when they were taken away from the water. Both of flooding promoted elongation of the leaves, restrained leaves to grow wider, and also restrained leaf formation of the plants except for S1 and S1 groups. Total leaf length of a plant decreased markedly after the second flooding which in S1 and S2 groups were 50.6% and 36.9% respectively less than that after the first flooding. Total leaf number of the plant in S1and S2 groups reduced significantly, and they were only 63.1% and 38.5% respectively of the control. Compared with the first flooding, total leaf length of a plant in the control increased signifi- cantly and decreased remarkably in S2 group after the second flooding. Furthermore, light response ability of the relative electronic transfer rate (rETR) in S1 group wasn't significantly different from the control, and rETRmax, in S2 group was significantly less than the control. Moreover, non- photochemical quenching (NPQ) decreased remarkably in S1 and S2 groups. It indicated that A. calamus had good restore ability of the light re- sponse, but restoring of its heat dissipation capacity was slower. [ Conclusion] Repeated flooding inhibited plant growth and population recovery of A. calamus under the dark condition.
文摘To study the effect of IL-18 and nitric oxide(NO) on the growth and metastasis of non-small cell lung cancer (NSCLC).Methods Serum IL-18 and nitrate and nitrite levels in 82 patients with NSCLC and 20 healthy control subjects were measured by using ELISA and Griess.Results The levels of serum IL-18 were (334.2±31.0)ng/L in NSCLC patients and (151.3±22.0)ng/L in control subjects,respectively.The levels of nitrate and nitrite were (237.1±21.0)μmol/L in NSCLC patients and (44.2±15.0)μmol/L in control subjects.The levels of serum IL-18 and nitrate and nitrite were not related with age,gender,histological types in patients with NSCLC.The levels of serum IL-18 was closely associated with TNM stage,lymph node metastasis and distal metastasis,but not with its degree and organ types of metastasis.There was a negative correlation between the levels of serum IL-18 and nitrate and nitrite.Conclusion Serum IL-18 and nitrate and nitrite levels may be useful to evaluate the prognosis of the patients with NSCLC.16 refs,2 tabs.
文摘试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组分别饲喂粗蛋白质水平为13.43%、15.04%、16.64%和18.25%的饲粮。试验期46 d,其中孵化期18 d (1~18 d),哺乳期28 d (19~46 d)。结果显示,试验Ⅱ组、Ⅲ组产蛋间隔显著低于试验Ⅰ组(P<0.05),试验Ⅲ组种蛋受精率显著高于试验Ⅰ组、Ⅳ组(P<0.05),试验Ⅲ组入孵蛋孵化率极显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.01)。试验Ⅲ组乳鸽28日龄体重显著高于试验Ⅰ组(P<0.05),乳鸽屠宰率极显著高于试验Ⅰ组(P<0.01),乳鸽胸肌率显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.05)。试验Ⅱ组、Ⅲ组、Ⅳ组窝料重比1极显著低于试验Ⅰ组(P<0.01)。研究表明,不同粗蛋白质水平影响了种鸽的繁殖性能、乳鸽的生长性能以及窝料重比,在“2+2”生产模式下,建议白王种鸽饲粮适宜粗蛋白质水平为16.64%。