Interference of Y-27632 on the signal transduction of transforming growth factor beta type 1 in ocular Tenon capsule fibroblasts

AIM: To investigate the interfering effect of Y-27632, a ROCK-I selective inhibitor, on the signal transduction pathway of transforming growth factor-beta 1 (TGF-beta 1) in ocular Tenon capsule fibroblasts (OTFS) in vitro. METHODS: After OTFS from passages 4 to 6 47 vitro were induced by TGF-beta 1 and then treated by Y-27632, the changes of the OTFS cell cycles were analyzed via flow cytometry, and the proteins expression of the alpha -smooth muscular actin (alpha -SMA), connective tissue growth factor (CTGF), collagen I were calculated by Western blot. After OTFS treated by the different concentrations of Y-27632, the expression levels of the alpha -SMA, CTGF and collagen I mRNA were assayed by RT-PCR. RESULTS: Y-27632 had no markedly effect on the OTFS cell cycles. After treated by TGF-beta 1, OTFS in G1 period significantly increased. The cell cycles distribution by both TGF-beta 1 and Y-27632 had no remarkable difference from that in control group. Y-27632 significantly inhibited the proteins expressions of both alpha -SMA and CTGF, while to some extent inhibited that of collagen I. TGF-beta 1 significantly promoted the proteins expressions of alpha -SMA, CTGF and collagen I. After OTFS treated by both TGF-beta 1 and Y-27632, of alpha -SMA, the protein expression was similar with that in control group (P=0.066>0.05), but the protein expression of CTGF or collagen I, respectively, was significantly different from that in control group (P=0.000<0.01). The differences of expressions of the alpha -SMA, CTGF and collagen I mRNA in 30, 150, 750 mu mol/L Y-27632 group were statistically significant, compared with those in control group, respectively (alpha -SMA, P=0.002, 0.000, 0.000; CTGF, P=0.014, 0.002, 0.001; collagen I,P=0.003, 0.002, 0.000). CONCLUSION: Blocking the Rho/ROCK signaling pathway by using of Y-27632 could inhibit the cellular proliferation and the expression of both CTGF and alpha -SMA whatever OTFS induced by TGF-beta 1 or not. Y-27632 suppressed the expression of collagen I mRNA without induction.

Human epidermal growth factor receptor type 2 protein expression in Chinese metastatic prostate cancer patients correlates with cancer specific survival and increases after exposure to hormonal therapy

Aim： To investigate human epidermal growth factor receptor type 2 （HER2） protein expression and gene amplification in Chinese metastatic prostate cancer patients and their potential value as prognostic factors. Methods： Immunohistochemistry （IHC） was performed to investigate HER2 protein expression in prostate biopsy specimens from 104 Chinese metastatic prostate cancer patients. After 3-11 months of hormonal therapy, 12 patients underwent transurethral resection of the prostate （TURP）. HER2 protein expression of TURP specimens was compared with that of the original biopsy specimens. Of these, 10 biopsy and 4 TURP specimens with HER2 IHC staining scores ≥ 2＋ were investigated for HER2 gene amplification status by fluorescent in situ hybridization （FISH）. Results： Of the 104 prostate biopsy specimens, HER2 protein expression was 0, 1＋, 2＋ and 3＋ in 49 （47.1%）, 45 （43.3%）, 8 （7.7%） and 2 （1.9%） cases, respectively. There was a significant association between HER2 expression and Gleason score （P = 0.026）. HER2 protein expression of prostate cancer tissues increased in 33.3% of patients after hormonal therapy. None of the 14 specimens with HER2 IHC scores 〉 2＋ showed HER2 gene amplification. Patients with HER2 scores 〉 2＋ had a significantly higher chance of dying from prostate cancer than those with HER2 scores of 0 （P = 0.004） and 1＋ （P = 0.034）. Multivariate Cox regression analysis showed that HER2 protein expression intensity was an independent predictor of cancer-related death （P = 0.039）. Conclusion： An HER2 IHC score 〉 2＋ should be defined as HER2 protein overexpression in prostate cancer. Overexpression of HER2 protein in cancer tissue might suggest an increased risk of dying from prostate cancer. HER2 protein expression increases in some individual patients after hormonal therapy.

Expression of serine protease SNC19/matriptase and its inhibitor hepatocyte growth factor activator inhibitor type 1 in normal and malignant tissues of gastrointestinal tract

AIM： To provide the expression profile of serine protease SNC19/matriptase and its inhibitor hepatocyte growth factor activator inhibitor type 1 （HAI-1） in normal and malignant tissues of gastrointestinal tract at mRNA level for further study on their correlations with tumor progression and metastasis. METHODS： Total RNAs were prepared from 37 samples of colorectal cancer tissues, 40 samples of gastric cancer tissues, and their adjacent normal tissues. The expression of SNC19/matriptase and HAI-1 in these samples was detected by real-time fluorescent quantitative PCR using glyceraldehyde-3-phosphate dehydrogenase as internal standard, and the clinical significance for the correlation with clinicopathological parameters was evaluated. RESULTS： In gastric cancer tissues the expression of HAI-1 and SNC19/matriptase was significantly lower than that in the corresponding adjacent normal tissues （Z = -3.280, P= 0.006; Z= -4.651, P= 0.000）. HAI-1：SNC19/matriptase ratio showed no difference between normal and malignant tissues （P〉0.05）. Analysis of clinicopathological parameters showed decreased expression of HAI-1 and HAI-1：SNC19/ matriptase ratio associated with stage Ⅲ/Ⅳ gastric tumors as compared to stage Ⅰ/Ⅱ ones （Z= -2.140, P= 0.031; Z = -2.155, P = 0.031）, and with lymph node-positive gastric cancer tissues as compared to lymph node-negative ones （Z = -2.081, P = 0.036; Z= -2.686, P = 0.006）. The expression of SNC19/matriptase had no relationship with stages and lymph node metastasis （P〉0.05）. The expression of HAI-1 and HAI-1：SNC19/matriptase ratio increased in well-differentiated gastric cancer tissues, but there was no statistical significance （P〉0.05）. The difference of SNC19/matriptase expression was not significant in gastric cancer tissues of different histological differentiation status （P〉0.05）. In colorectal cancer tissues, the expression of HAI-1 and SNC19/matriptase was also markedly lower than that in their adjacent normal tissues （Z= -3.100, P = 0.002; Z= -2.731, P = 0.006）, whereas HAI-1：SNC19/matriptase ratio showed no difference. Decreased expression of HAI-1 was associated with increased invasive depth and lymph node metastasis, but there was no statistical significance （P〉0.05）. The difference of SNC19/matriptase expression and HAI-1： SNC19/matriptase ratio was not significant in different stages and different lymph node metastasis status （P〉0.05）. The expression of SNC19/matriptase, HAI-1 or HAI-1： SNC19/matriptase ratio showed no difference in colorectal cancer tissues of different histological differentiation status （P〉0.05）. CONCLUSION： The expressions of SNC19/matriptase and its inhibitor HAI-1 are decreased in gastrointestinal cancer tissues compared to their normal counterparts, and the decreased expression of HAI-1 may correlate with invasion and lymph node metastasis. The possible mechanisms involved need to be further investigated.

KIT and platelet-derived growth factor receptor α wild-type gastrointestinal stromal tumor associated with neurofibromatosis type 1: Two case reports

BACKGROUND Gastrointestinal stromal tumors(GISTs) associated with neurofibromatosis are uncommon compared to their gastrointestinal counterparts. Patients with neurofibromatosis type 1(NF-1) have an increased risk of developing gastrointestinal tumors, including rare types such as GIST.CASE SUMMARY A 60-year-old male Chinese patient was diagnosed with NF-1 10 years ago and presented with upper abdominal discomfort and black stools. Endoscopic ultrasonography and an enhanced abdominal computed tomography scan revealed a mass located 4 cm from the muscular layer of the descending duodenum. A 59-year-old Chinese woman who was diagnosed with NF-1 25 years ago presented with sudden unconsciousness and black stools. Multiple masses in the duodenum were noted by echogastroscopy and an enhanced abdominal computed tomography scan. Both patients presented with cutaneous neurofibromas. The histologic examination of tumors from both patients revealed spindle cells and low mitotic activity. Immunohistochemically, the tumor cells showed strong positivity for KIT(CD117), DOG-1, CD34, and Dehydrogenase Complex Subunit B, and negativity for SMA, desmin, S-100, and β-catenin. None of the six tumors from two patients had KIT exon 9, 11, 13, or 17 or platelet-derived growth factor receptor α exon 12 or 18 mutation, which is a typical finding for sporadic GISTs. None of the six tumors from the two patients had a BRAFV600 E mutation. The patients were alive and well during the follow-up period(range:0.6-5 yr).CONCLUSION There have been only a few previous reports of GISTs associated with NF-1.Although GISTs associated with NF-1 have morphologic and immunohistochemical similarities with GISTs, the pathogenesis, incidence,genetic background, and prognosis are not completely known. A medical history of NF-1 in a patient who has gastrointestinal bleeding or anemia and an intraabdominal mass with nonspecific computed tomography features may help in diagnosing GIST by virtue of the well-known association of these two entities.Molecular genetic studies of cases indicated that GISTs in NF-1 patients have a different pathogenesis than sporadic GISTs.

Inhibition of Ubiquitin-specific Protease 4 Attenuates Epithelial-Mesenchymal Transition of Renal Tubular Epithelial Cells via Transforming Growth Factor Beta Receptor Type Ⅰ

Objective Ubiquitin-specific protease 4(USP4)facilitates the development of transforming growth factor-beta 1(TGF-β1)-induced epithelial-mesenchymal transition(EMT)in various cancer cells.Moreover,EMT of renal tubular epithelial cells(RTECs)is required for the progression of renal interstitial fibrosis.However,the role of USP4 in EMT of RTECs remains unknown.The present study aimed to explore the effect of USP4 on the EMT of RTECs as well as the involved mechanism.Methods In established unilateral ureteral obstruction(UUO)rats and NRK-52E cells,immunohistochemistry and Western blot assays were performed.Results USP4 expression was increased significantly with obstruction time.In NRK-52E cells stimulated by TGF-β1,USP4 expression was increased in a time-dependent manner.In addition,USP4 silencing with specific siRNA indicated that USP4 protein was suppressed effectively.Meanwhile,USP4 siRNA treatment restored E-cadherin and weakened alpha smooth muscle actin(α-SMA)expression,indicating that USP4 may promote EMT.After treatment with USP4 siRNA and TGF-β1 for 24 h,the expression of TGF-β1 receptor type I(TβRI)was decreased.Conclusion USP4 promotes the EMT of RTECs through upregulating TβRI,thereby facilitating renal interstitial fibrosis.These findings may provide a potential target of USP4 in the treatment of renal fibrosis.

Effects of connective tissue growth factor and collagen type Ⅰ scleroderma

Objective： To investigate the effects of connective tissue growth factor（CTGF） and collagen type I（COL-I） on the pathogenesis of scleroderma and explore the relationship between the level of COL-I and CTGF. Methods： 12 mice model of scleroderma was established by the injection of Bleomycin. The level of CTGF and COL-I were detected by immunohistochemical method. The relationship was analyzed between CTGF and COL-I level. As control group, 12 healthy mice were selected. Results： The levels of CTGF and COL-I in sclerotic models were higher than in normal controls （P 〈 0.05）. It was found that there was a correlation between the level of CTGF and COL-I. Conclusion： CTGF and COL-I played an important role in the hardening process of the skin lesions of the mice model, which may be involved in the pathogenesis of scleroderma.

Association of hepatocyte-derived growth factor receptor/caudal type homeobox 2 co-expression with mucosal regeneration in active ulcerative colitis

AIM:To characterize the regeneration-associated stem cell-related phenotype of hepatocyte-derived growth factor receptor(HGFR)-expressing cells in active ulcerative colitis(UC).METHODS:On the whole 38 peripheral blood samples and 38 colonic biopsy samples from 18 patients with histologically proven active UC and 20 healthy control subjects were collected.After preparing tissue microarrays and blood smears HGFR,caudal type homeobox 2(CDX2),prominin-1(CD133) and Musashi-1conventional and double fluorescent immunolabelings were performed.Immunostained samples were digitalized using high-resolution Mirax Desk instrument,and analyzed with the Mirax TMA Module software.For semiquantitative counting of immunopositive lamina propria(LP) cells 5 fields of view were counted at magnification x 200 in each sample core,then mean ± SD were determined.In case of peripheral blood smears,30 fields of view with 100 μm diameter were evaluated in every sample and the number of immunopositive cells(mean ± SD) was determined.Using 337 nm UVA Laser MicroDissection system at least 5000 subepithelial cells from the lamina propria were collected.Gene expression analysis of HGFR,CDX2,CD133,leucine-rich repeat-containing G-protein coupled receptor 5(Lgr5),Musashi-1 and cytokeratin20(CK20) were performed in both laser-microdisscted samples and blood samples by using real time reverse transcription polymerase chain reaction(RT-PCR).RESULTS:By performing conventional and double fluorescent immunolabelings confirmed by RT-PCR,higher number of HGFR(blood:6.7 ± 1.22 vs 38.5 ±3.18;LP:2.25 ± 0.85 vs 9.22 ± 0.65;P < 0.05),CDX2(blood:0 vs 0.94 ± 0.64;LP:0.75 ± 0.55 vs 2.11± 0.75;P < 0.05),CD133(blood:1.1 ± 0.72 vs 8.3± 1.08;LP:11.1 ± 0.85 vs 26.28 ± 1.71;P < 0.05)and Musashi-1(blood and LP:0 vs scattered) positive cells were detected in blood and lamina propria of UC samples as compared to controls.HGFR/CDX2(blood:0 vs 1± 0.59;LP:0.8 ± 0.69 vs 2.06 ± 0.72,P < 0.05)and Musashi-1/CDX2(blood and LP:0 vs scattered) coexpressions were found in blood and lamina propria of UC samples.HGFR/CD133 and CD133/CDX2 coexpressions appeared only in UC lamina propria samples.CDX2,Lgr5 and Musashi-1 expressions in UC blood samples were not accompanied by CK20 mRNA expression.CONCLUSION:In active UC,a portion of circulating HGFR-expressing cells are committed to the epithelial lineage,and may participate in mucosal regeneration by undergoing mesenchymal-to-epithelial transition.

Characterization of Growth and Light Utilization for Rice Genotypes with Different Tiller Angles

Tiller angle is very important for plant architecture and canopy structure in rice （Oryza sativa L.）. Physiological and ecological characteristics of three rice genotypes with different tiller angle habits were compared in the paper. DI508, a genotype with changing tiller angle during the growth, has semi-erect tillers at early tillering stage, similar to genotype M09, and had erect tillers at late stage, similar to genotype 9308. In terms of dry biomass per plant, DI508 was consistently higher than those of M09 and 9308 throughout the growth. It was also a distinct difference of leaf area per plant that DI508 was larger than two others. From booting stage, DI508 and 9308 maintained higher photosynthetic ability of the topmost three leaves, while M09 showed rapid decline in photosynthesis during grain filling. It may be concluded that the genotype DI508 with dynamic tiller angle habit has a comprehensive advantage of fast growth and high weed competition at early stage and slow decline in photosynthesis at late stage.

Nerve growth factor and inducible nitric oxide synthase expression in the mesencephalon and diencephalon, as well as visual-and auditory-related nervous tissues, in a macaque model of type 2 diabetes

The present study detected distribution and expression of nerve growth factor and inducible nitric oxide synthase in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a macaque model of type 2 diabetes using immunohistochemistry. Results showed that nerve growth factor expression decreased, but inducible nitric oxide synthase expression increased, in the mesencephalon and diencephalon, as well as visual- and auditory- related nervous tissues. These results suggested that nerve growth factor and inducible nitric oxide synthase play an important role in regulating the development of diabetic visual- and auditory-related diseases.

Growth Performance of Different Cross Combinations of Quality Lean Type Pig

Ten healthy weaning piglets of Landrace x Yorkshire （LY） in good body condition with body weight of （24 ± 1.27） kg were selected, and ten weaning piglets of Berkshire × LY （BLY） and Duroc × LY （DLY） with similar situation were also selected respectively to study the growth performance of different cross combinations. Three groups of experimental pigs were bred with conventional diet. The results showed that the average dry matter intakes of combinations of BLY and DLY were slightly decreased by 4.97% and 5.47% compared with the combination of LY, while average daily gain was significantly increased by 3.13% and 1.14%, and feed-weight ratio was decreased by 7.94% and 6.35%, respectively; economic benefit per pig was increased by 0.61 and 0.41 yuan, respectively. The combinations of BLY and DLY have better growth performance, which can be promoted in the production.

Rabbit Intensification Systems in Rwanda: Feeding Influence and Growth

A study was conducted in Northern Province of Rwanda, from the College of Agriculture and Veterinary Medicine, Busogo Campus located in Musanze district to evaluate the effect of feed type on rabbit growth in rabbit intensification systems in Rwanda. The Complete Randomized Design (CRD) was used and data were collected on rabbit growth weekly for a period of 12 weeks. The experiment was composed of three treatments replicated ten times. The treatments included three types of feeds namely;cabbage combined with Mucuna pruriens added to local forage (I), cabbages combined with Leucaena leucocephala added to local forage (II) and a control composed of other varieties of locally available forage, such as Bidens pilosa, Crassocephalum vitellium and Galinsoga parviflora (III) which was considered as the control (Farmers practice). The feeds were given to ten rabbits separated in individual cages, and each rabbit was considered a replicate. Water was given ad libitum. One month old rabbits (weaners) were used and data were collected after one week of adaptation for 12 weeks. The results showed that the mean of weight gain after 12 weeks was 783.3 g, 760.7 g and 705.7 g for feed type I, II and III respectively. The difference between means of feed types after 12 weeks was not significant (p > 0.5), which implied that rabbit growth did not depend on the feed type. The mean weight gain after 8 weeks was 707.5 g, 661.4 g and 577.1 g for feed type I, II and III respectively. At 8 weeks, the difference between means of feed types was significant (p Mucuna pruriens combined with cabbage and local forage were growing faster than rabbits from other treatments at 8 weeks. The researchers recommended that farmers should be facilitated with feeding materials by the concerned institutions. Training of rabbit farmers and further researches on locally available feeding materials were also given as recommendations at the end of this study.

新型工业化与人类文明新形态

人类发展和文明形态的演变和繁荣,表现为艰难地开拓畅通之途。不同文明形态间的融合畅通意味着人类文明的更大进步。人类文明进程所面临的根本问题是,多样化的文明形态如何实现相互畅通、共存共荣?工业化的基本逻辑:机器替代人力,创新改变世界;无论“陆权”“海权”,工业能力是实权。新型工业化的经济学叙事原则就是:脱胎于传统工业化,又升华自传统工业化;扬弃传统工业化之弊端,又发扬传统工业化之张力。经济全球化多彩新格局的形成,可以获得中国创造的人类文明新形态的建构性支撑。同时,中国的新型工业化也可以获得经济全球化新格局的更大空间和多样化体系拓展。中国新型工业化是经济全球化新格局的创造性力量,不仅创造中华文明新形态,而且促进协调解决了世界不同文明形态的共存共荣问题。经济全球化需要建立和维护的是“安全畅通、规则平等、承认差异”的世界市场经济秩序。从经济学范式的底层逻辑说,必须从工具理性的一维执念变革为理性、文化、制度三维范式承诺,以体现人类文明新形态内涵的丰富性。

育苗容器对紫叶紫薇容器苗生长、开花及生理的影响

【目的】探讨育苗容器类型和规格对紫叶紫薇容器苗生长、开花及生理的影响,筛选出培育紫叶紫薇容器苗的适宜育苗容器,为高效培育紫薇容器苗提供理论依据。【方法】以紫叶紫薇优良新品种‘丹红紫叶’2年生苗木为研究材料,选用黑色塑料营养杯(C1)、白色无纺布美植袋(C2)、黑色控根容器(C3)3个类型以及16×16(D1)、21×21(D2)、25×25(D3)3种规格(直径×高,cm),组成9种容器组合处理,测定不同处理容器苗生长、开花及生理指标。【结果】(1)不同育苗容器处理对紫叶紫薇容器苗的生长、开花及生理指标均影响显著,同一容器类型苗木的生长、根系、生物量、花期、花径、花序、可溶性蛋白含量、可溶性糖含量、相对叶绿素含量、净光合速率、气孔导度、蒸腾速率等指标均随着育苗容器规格的增大而显著增加。(2)地径生长量、总根长、总根表面积、总根体积、根尖数、地上部干质量、地下部干质量、总干质量、花期均以C3D3处理最大,比最小的C1D1处理分别显著提高817.12%、108.12%、94.60%、75.66%、144.14%、135.67%、228.45%、164.65%、34.48%;苗高生长量、冠幅生长量、叶面积、花序长和宽均以C1D3处理最高,比最低的C1D1处理分别显著提高116.05%、81.39%、114.95%、70.44%和65.79%;相对叶绿素含量、水分利用效率均以C3D3处理最大,可溶性蛋白和可溶性糖含量、净光合速率、气孔导度均以C1D3处理最高;胞间CO_(2)浓度、水分利用效率在各处理之间无显著差异。(3)各处理隶属函数法综合评价得分排序为:C3D3>C1D3>C2D3>C3D2>C2D2>C1D2>C2D1>C3D1>C1D1。【结论】黑色控根容器在地径生长量、根系生长指标、生物量、花期和花径、叶绿素相对含量及净光合速率方面表现最佳;紫叶紫薇2年生苗的最适宜育苗容器是直径25 cm、高25 cm的黑色控根容器。

母猪饲粮添加益生菌和合生元对子代巴马香猪肌肉氨基酸组成及生长发育相关基因表达的影响

[目的]本文旨在研究母猪饲粮添加益生菌和合生元对子代巴马香猪肌肉氨基酸组成及相关基因表达的影响。[方法]选用64头妊娠巴马香猪,随机分为对照组(基础饲粮)、抗生素组(50 g·t^(-1)维吉尼亚霉素)、益生菌组(200 mL·d^(-1)益生菌发酵液)和合生元组(500 g·t^(-1)低聚木糖+200 mL·d^(-1)益生菌发酵液),每组16头母猪,单栏饲养。母猪在整个妊娠期和哺乳期饲喂相应试验饲粮;仔猪断奶后,每窝选取2头仔猪,每组32头(8栏,每栏4头),饲喂基础饲粮。分别于65、95和125日龄每组选取8头采集股二头肌和腰大肌样品,测定其水解氨基酸组成和相关基因的表达量。[结果]与对照组相比,合生元组子代巴马香猪股二头肌粗蛋白、Gly、Ser、Ala、Asp、Glu、Pro和Ile含量,腰大肌Gly、Glu、Ser和His含量均显著增加(P<0.05);益生菌组子代巴马香猪股二头肌肌肉萎缩F-box蛋白32和生肌调节因子(MyoG)、腰大肌生肌因子5(Myf5)的表达水平显著上调(P<0.05),腰大肌肌球蛋白重链Ⅱb(MyHCⅡb)、股二头肌和腰大肌肌肉生长抑制素(MSTN)的表达水平显著下调(P<0.05);合生元组子代巴马香猪股二头肌Myf6、生肌分化因子和MyoG以及腰大肌Myf5和MyoG的表达水平显著上调(P<0.05),股二头肌MSTN的表达水平显著下调(P<0.05);益生菌组和合生元组子代巴马香猪股二头肌MyHCⅡx的表达水平显著下调(P<0.05);抗生素组子代巴马香猪腰大肌MyHCⅡx的表达水平显著上调(P<0.05),而股二头肌MyHCⅠ和腰大肌MyHCⅡa的表达水平显著下调(P<0.05)。[结论]母猪饲粮添加益生菌和合生元可改变子代巴马香猪肌肉氨基酸组成,调控生肌因子相关基因表达,上调氧化型肌纤维基因的表达、下调酵解型肌纤维基因的表达,从而有利于肉品质的改善。

南方型黑杨生长性状和木材材性与湿心材形成的相关性

为了分析南方型黑杨生长性状、木材性状对其湿心材形成的影响程度,于2023年11月份,在岳阳市君山区丁字堤南方型黑杨试验林,选取13年生的8个南方型黑杨无性系为研究对象,每个无性系选取6株样株,用9 mm生长锥在树高1.3 m处钻取木样,分别测量木芯长度、湿心材长度,并称量质量;依据试验设计,测定样木的胸径、树高、材积、树液pH、生材含水量、气干材含水量、木材基本密度;运用相关分析法、通径分析法,计算各性状对湿心材比例的直接影响、间接影响、决定系数,分析南方型黑杨无性系生长性状、木材性状的7个性状指标对湿心材比例的影响。结果表明:8个无性系间,湿心材比例、胸径、树高、材积、生材含水量存在极显著差异。树液pH和生材含水量,与湿心材比例存在极显著正相关,气干材含水量与湿心材比例存在显著正相关。通径分析表明,生材含水量(W r)、树液pH、气干材含水量(W a)是影响湿心材比例(R(wh))的重要因子,通过多元回归分析建立3个性状与湿心材比例的多元回归方程R(wh)=-0.995+0.006pH+0.075 W r+0.005 W a;其中,生材含水量对湿心材比例的直接影响(0.522)和单独决定系数大小表明,生材含水量对湿心材比例影响最大。因此,生材含水量大小可以作为选育南方型黑杨低湿心材选育时的评定指标。

不同立地条件对两种楠属苗木生长量与光合特性的影响

【目的】了解不同立地条件下楠属植物的生长量及光合特性,进一步探讨立地条件对紫楠和白楠容器苗生长发育习性的影响。【方法】本试验在荆州地区对紫楠和白楠容器苗进行研究。选取树龄3 a的紫楠和白楠容器苗分别移栽至弥市、松滋、太湖,并对其株高、地径的生长量和Pn、Tr以及叶绿素含量等指标进行测定、比较和分析。【结果】种植于弥市的紫楠和白楠苗高平均生长量分别为59、42.3 cm,地径平均生长量为0.497、0.538 cm,而种植于太湖的紫楠和白楠苗高平均生长量分别为7.733、7.067 cm,地径平均生长量为0.212、0.208 cm,可见两种楠木在弥市生长状况最好,在太湖的生长状况最差,且紫楠生长速度比白楠快;通过对两种楠木与土壤化学性质的相关性分析,发现其与土壤中氮元素、磷元素的相关性系数最高,紫楠和白楠净光合速率、蒸腾作用、水分利用率、叶片含氮量与δ^(15)N均易受到土壤施肥等外界因素的短期影响,而叶绿素和δ^(13)C为植株长期生长情况的积累。【结论】紫楠和白楠种植于弥市的生长情况最好,太湖最差,且紫楠的生长速度快于白楠,但白楠更适宜在强光环境下生长。叶片叶绿素和δ^(13)C含量可用于初步判断楠属植物生长情况。

基于TGF-β1/Smad信号通路探究度拉糖肽对2型糖尿病肾病大鼠肾损伤的影响及机制

目的:探讨基于转化生长因子-β(TGF-β1)/Smad信号通路度拉糖肽(Dula)对2型糖尿病肾病(T2DN)大鼠肾损伤的影响。方法:随机选取10只大鼠作为Control组,其余大鼠构建T2DN模型,将建模成功的大鼠随机分为T2DN组、L-Dula、M-Dula、H-Dula,Dula+SRI-011381每组10只。测定各组大鼠体质量;采用全自动生化分析仪检测T2DN大鼠血清中ALB、Scr、BUN、TG、TC含量;HE和PAS染色观察T2DN大鼠肾组织病理改变;Western blot检测T2DN大鼠肾组织中TGF-β1、Smad2/3、Smad7蛋白的表达。结果:T2DN组肾小球肥大,基底膜增厚,囊腔萎缩,间质炎性细胞浸润,肾小球内糖原沉积增多,可见红色染色区域,24h尿蛋白、Scr、BUN、FBG、TG、TC、TGF-β1、Smad2/3表达高于Control组,体质量、Smad7表达低于Control组(P<0.05);L-Dula、M-Dula、H-Dula组肾小球和肾小管病变程度均依次减轻,红色染色区域依次减少,24h尿蛋白、Scr、BUN、FBG、TG、TC、TGF-β_(1)、Smad2/3表达低于T2DN组,体质量、Smad7表达高于T2DN组(P<0.05);Dula+SRI-011381组肾小球和肾小管病变加重、红色染色区域增大、24h尿蛋白、Scr、BUN、FBG、TG、TC、TGF-β1、Smad2/3表达高于H-Dula组,体质量、Smad7表达低于H-Dula组(P<0.05)。结论:Dula可能通过抑制TGF-β1/Smad信号通路抑制T2DN大鼠肾损伤。

椰糠型生态保育基质栽培对辣椒品质及产量的影响

以‘甘科4号’辣椒为试材,以当地常规栽培作为对照(CK),采用温室大棚栽培的试验方法,研究椰糠、风沙土、人造泥炭、有机肥配比分别为5∶2∶1∶3(T1)、4∶3∶1∶3(T2)、3.5∶3.5∶1∶3(T3)、3∶4∶1∶3(T4)、2∶5∶1∶3(T5)、1∶6∶1∶3(T6)、0∶7∶1∶3(T7)复混形成的模块化生态保育基质对辣椒生长特性及产量的影响,以期为探索西部荒漠戈壁工业化土壤改良及特色生态农业发展提供新路径。结果表明:不同生育期下,T2~T5处理可促进辣椒长势(株高、茎粗、株幅),根系、品质和产量亦显著提高,且各项指标均随椰糠含量增加呈现先增大后降低的趋势;相较CK,T5处理下单株结果数和单株产量分别增加了9.47%、19.65%。利用VIKOR法对各处理的品质及产量进行综合评价显示,T5处理下辣椒品质及产量最优,利益比率最小,为0.019。研究结果进一步证明椰糠型生态保育基质(椰糠含量占比30%~55%)适合荒漠化土地上的辣椒种植。

不同料型对育肥期德州驴生长性能、屠宰性能和肉品质的影响

本试验旨在研究颗粒型全混合日粮(TMR)和散状TMR对育肥期德州驴生长性能、屠宰性能和肉品质的影响。选取体重[(150±2) kg]相近、年龄为8个月的健康公驴20头,随机分为2组,每组10个重复。对照组(CG组)饲喂散状TMR,试验组(EG组)饲喂颗粒型TMR,2种不同料型饲粮组成与营养水平一致,均自由采食,预试期10 d,正试期129 d。结果表明:1)EG组末重显著高于CG组(P<0.05);EG组平均日采食量极显著高于CG组(P<0.01);EG组平均日增重显著高于CG组(P<0.05);EG组增重效益显著高于CG组(P<0.05)。2)EG组和CG组宰前活重无显著差异(P>0.05),但EG组胴体重与屠宰率显著高于CG组(P<0.05),EG组屠宰效益趋于显著地高于CG组(0.05<P<0.10)。3) EG组肌肉剪切力显著小于CG组(P<0.05),EG组和CG组肌肉蒸煮损失、滴水损失和肉色均无显著差异(P>0.05)。4) EG组肌肉中粗脂肪(EE)含量显著高于CG组(P<0.05),EG组和CG组肌肉中干物质(DM)、粗蛋白质(CP)、有机物(OM)和粗灰分(Ash)含量无显著差异(P>0.05)。5) EG组肌肉中组氨酸(His)和羟基脯氨酸(Hyp)含量显著高于CG组(P<0.05),EG组和CG组肌肉中必需氨基酸(EAA)、半必需氨基酸(SEAA)、非必需氨基酸(NEAA)、风味氨基酸(FAA)含量和EAA/NEAA值无显著差异(P>0.05)。EG组肌肉中辛酸(C8∶0)和反-9-十八碳烯酸(C18∶1n9t)含量显著高于CG组(P<0.05);EG组肌肉中饱和脂肪酸(SFA)含量显著低于CG组(P<0.05),EG组肌肉中单不饱和脂肪酸(MUFA)含量和MUFA/SFA值显著高于CG组(P<0.05),EG组肌肉中不饱和脂肪酸(UFA)含量显著高于CG组(P<0.05)。由此可见,与散状TMR相比,颗粒型TMR极显著提高育肥期德州驴平均日采食量,显著提高平均日增重、屠宰率、经济效益;提高驴肉嫩度并改善驴肉的脂肪酸组成。综上所述,在规模化养殖中应用颗粒型TMR来育肥德州驴是可行且高效的。

增温背景下不同土壤对冬小麦产量品质影响评价

利用冬小麦品种‘镇麦168’,以黄棕壤、砂礓黑土、风沙土、红壤、潮土、黑土、黄土、灰钙土、紫色土、砖红壤、盐碱土和棕壤共12种典型农田土壤为基质,在开放式增温系统开展模拟大气增温框栽试验。试验设置常温对照(CK)和增温1.5℃(eT)两个处理,增温处理为冬小麦全生育期增温。以成熟期冬小麦单穗粒数、千粒重等表征产量变化,籽粒淀粉、蛋白质及其组分等营养指标体现品质构成。结果表明:(1)全生育期增温分别使黄棕壤、风沙土、黑土、黄土、灰钙土和紫色土冬小麦较常温对照减产33.82%、20.96%、16.60%、55.92%、28.45%和21.19%,但潮土冬小麦增产16.13%(P<0.05),其他土壤条件下冬小麦产量无明显变化。(2)就冬小麦营养品质,直链淀粉和支链淀粉含量在增温作用下较常温对照存在不同程度下降,且直链淀粉降幅大于支链淀粉,在红壤、黑土、黄土、灰钙土、紫色土、盐碱土和棕壤条件下冬小麦总淀粉含量显著降低(P<0.05)。籽粒蛋白质及其组分,清蛋白和球蛋白呈显著增加趋势,而醇溶蛋白和谷蛋白显著下降,且降幅大于前两者增幅,除红壤冬小麦总蛋白含量无显著变化以外,其他11种土壤条件下冬小麦总蛋白含量均较常温对照显著降低(P<0.05)。冬小麦可溶性总糖仅在潮土、灰钙土和紫色土条件下显示出显著增加趋势(P<0.05)。(3)利用隶属函数对常温对照和增温各处理进行综合品质排名,表现最好的为常温组黑土冬小麦(U=0.707),常温组中棕壤(U=0.691)、灰钙土(U=0.647)、紫色土(U=0.644)和黄土冬小麦(U=0.644)次之,品质最差的是常温下红壤冬小麦(U=0.364)和增温下红壤冬小麦(U=0.368)。除潮土冬小麦以外,其他11种土壤冬小麦品质均表现为增温劣于对照。(4)常温条件下冬小麦产量最大影响因素为单穗粒数,直接通径系数为0.630,其次为有效穗数和球蛋白,均体现为直接作用,直接通径系数分别为0.538和-0.118;增温条件下冬小麦产量最大影响因素也是单穗粒数,直接通径系数为0.603,其次为有效穗数、千粒重和总淀粉,有效穗数和总淀粉通过与单穗粒数的间接作用对冬小麦产量产生影响,间接通径系数分别为0.322和0.381。综合而言,增温通过对冬小麦产量构成和籽粒营养成分的综合作用影响品质,12种典型农田土壤中,潮土冬小麦对增温表现为正效应,其他土壤为负效应,冬小麦产量品质形成对气候变暖响应受到土壤类型的调控。