Objective: To investigate the electrophysiological changes of autonomic cells in left ventricular outflow tract in guinea pigs with iron deficiency anemia complicated with chronic heart failure.Methods: Guinea pigs mo...Objective: To investigate the electrophysiological changes of autonomic cells in left ventricular outflow tract in guinea pigs with iron deficiency anemia complicated with chronic heart failure.Methods: Guinea pigs model of iron deficiency anemia complicated with chronic heart failure in 10 guinea pigs of the experimental group was made by feeding a low iron diet,pure water and subcutaneous injection of isoproterenol. The control group consisting of 11 guinea pigs was given normal food, normal water and injected with normal saline. The left ventricular outflow tract model specimen was also prepared. The standard microelectrode technique was used to observe electrophysiological changes of autonomic cells in the outflow tract of left ventricular heart failure complicated with iron deficiency anemia in guinea pig model. The indicators of observation were maximal diastolic potential, action potential amplitude, 0 phase maximal depolarization velocity, 4 phase automatic depolarization velocity, repolarization 50% and 90%, and spontaneous discharge frequency.Results: Compared with the control group, 4 phase automatic depolarization velocity,spontaneous discharge frequency and 0 phase maximal depolarization velocity decreased significantly(P < 0.01) and action potential amplitude reduced(P < 0.01) in model group. Moreover, repolarization 50% and 90% increased(P < 0.01).Conclusions: There are electrophysiological abnormalities of the left ventricular outflow tract in guinea pigs with iron deficiency anemia complicated with heart failure.展开更多
Objective:To investigate the changes of left ventricular papillary muscle action potentials in guinea pigs with iron deficiency anemia and heart failure.Methods:A total of 20 cases of iron deficiency anemia with heart...Objective:To investigate the changes of left ventricular papillary muscle action potentials in guinea pigs with iron deficiency anemia and heart failure.Methods:A total of 20 cases of iron deficiency anemia with heart failure were treated with experimental group and 10 normal guinea pigs as control group. Blood samples were collected to determine hemoglobin content, red blood cell number and whole blood iron index, and the changes of cardiac function and hemodynamics were detected by 6240 biological signal collection system to determine whether the model was successful or not, Intracellular microelectrode technique was used to determine the action potentials of the papillary muscles in the model group and the control group. the potential amplitudes (APA), overshoot values (APA), maximum depolarization rate (Vmax), 20 % of repolarization, 50 % and 90 % of repolarization (APD20, APD50 and APD90 ) and the average velocity of repolarization were measured. Compare statistical difference between the model group and the control group.Results: 14 cases of model group survived completely, compared with control group, APD50 and APD90 prolonged, and the average velocity decreased.Conclusions: the action potential repolarization duration in the guinea pig papillary muscle of iron deficiency anemia with heart failure is prolonged, and the average repolarization velocity is slow.展开更多
目的:观察肾虚对豚鼠耳蜗血管纹毛细血管及听功能的影响,为进一步研究中医"肾开窍于耳"的理论以及阐明肾虚致聋机制提供实验基础。方法:筛选健康活泼、耳廓反射灵敏的白色红目豚鼠40只,随机分成肾虚模型组和正常对照组,利用...目的:观察肾虚对豚鼠耳蜗血管纹毛细血管及听功能的影响,为进一步研究中医"肾开窍于耳"的理论以及阐明肾虚致聋机制提供实验基础。方法:筛选健康活泼、耳廓反射灵敏的白色红目豚鼠40只,随机分成肾虚模型组和正常对照组,利用糖皮质激素制备豚鼠"肾虚"模型,造模完成后测试各组豚鼠ABR阈值以及90 dB SPL给声强度下Ⅲ波潜伏期。断头处死豚鼠,取出各组听泡,苏木素伊红染色后进行血管纹铺片,显微镜下观察血管纹毛细血管病理改变及计算各组铺片的血管纹毛细血管面积与视野面积的比值。结果:(1)造模后肾虚模型组豚鼠ABR阈值显著提高,且90 dB SPL给声强度下Ⅲ波潜伏期明显延后,两者与正常对照组相比有显著性差异(P<0.01);(2)肾虚模型组豚鼠耳蜗血管纹毛细血管出现断流或红细胞淤积现象,且其毛细血管面积比值明显小于对照组,统计结果显示两者相比有显著性差异(P<0.05)。结论:肾虚可引起耳蜗血管纹毛细血管面积比值降低、血管收缩,出现断流或红细胞淤积现象,有可能是造成听功能减退的原因之一。展开更多
基金supported by Zhangjiakou Project of Science and Technology Studies and Development Planning(Grand No.1321078D)
文摘Objective: To investigate the electrophysiological changes of autonomic cells in left ventricular outflow tract in guinea pigs with iron deficiency anemia complicated with chronic heart failure.Methods: Guinea pigs model of iron deficiency anemia complicated with chronic heart failure in 10 guinea pigs of the experimental group was made by feeding a low iron diet,pure water and subcutaneous injection of isoproterenol. The control group consisting of 11 guinea pigs was given normal food, normal water and injected with normal saline. The left ventricular outflow tract model specimen was also prepared. The standard microelectrode technique was used to observe electrophysiological changes of autonomic cells in the outflow tract of left ventricular heart failure complicated with iron deficiency anemia in guinea pig model. The indicators of observation were maximal diastolic potential, action potential amplitude, 0 phase maximal depolarization velocity, 4 phase automatic depolarization velocity, repolarization 50% and 90%, and spontaneous discharge frequency.Results: Compared with the control group, 4 phase automatic depolarization velocity,spontaneous discharge frequency and 0 phase maximal depolarization velocity decreased significantly(P < 0.01) and action potential amplitude reduced(P < 0.01) in model group. Moreover, repolarization 50% and 90% increased(P < 0.01).Conclusions: There are electrophysiological abnormalities of the left ventricular outflow tract in guinea pigs with iron deficiency anemia complicated with heart failure.
文摘Objective:To investigate the changes of left ventricular papillary muscle action potentials in guinea pigs with iron deficiency anemia and heart failure.Methods:A total of 20 cases of iron deficiency anemia with heart failure were treated with experimental group and 10 normal guinea pigs as control group. Blood samples were collected to determine hemoglobin content, red blood cell number and whole blood iron index, and the changes of cardiac function and hemodynamics were detected by 6240 biological signal collection system to determine whether the model was successful or not, Intracellular microelectrode technique was used to determine the action potentials of the papillary muscles in the model group and the control group. the potential amplitudes (APA), overshoot values (APA), maximum depolarization rate (Vmax), 20 % of repolarization, 50 % and 90 % of repolarization (APD20, APD50 and APD90 ) and the average velocity of repolarization were measured. Compare statistical difference between the model group and the control group.Results: 14 cases of model group survived completely, compared with control group, APD50 and APD90 prolonged, and the average velocity decreased.Conclusions: the action potential repolarization duration in the guinea pig papillary muscle of iron deficiency anemia with heart failure is prolonged, and the average repolarization velocity is slow.
文摘目的:观察肾虚对豚鼠耳蜗血管纹毛细血管及听功能的影响,为进一步研究中医"肾开窍于耳"的理论以及阐明肾虚致聋机制提供实验基础。方法:筛选健康活泼、耳廓反射灵敏的白色红目豚鼠40只,随机分成肾虚模型组和正常对照组,利用糖皮质激素制备豚鼠"肾虚"模型,造模完成后测试各组豚鼠ABR阈值以及90 dB SPL给声强度下Ⅲ波潜伏期。断头处死豚鼠,取出各组听泡,苏木素伊红染色后进行血管纹铺片,显微镜下观察血管纹毛细血管病理改变及计算各组铺片的血管纹毛细血管面积与视野面积的比值。结果:(1)造模后肾虚模型组豚鼠ABR阈值显著提高,且90 dB SPL给声强度下Ⅲ波潜伏期明显延后,两者与正常对照组相比有显著性差异(P<0.01);(2)肾虚模型组豚鼠耳蜗血管纹毛细血管出现断流或红细胞淤积现象,且其毛细血管面积比值明显小于对照组,统计结果显示两者相比有显著性差异(P<0.05)。结论:肾虚可引起耳蜗血管纹毛细血管面积比值降低、血管收缩,出现断流或红细胞淤积现象,有可能是造成听功能减退的原因之一。
