Experimental Studies of the Therapeutic Effect of Gypsophila Oldhamiana Gypsogenin on Lewis Lung Cancer in Mice

OBJECTIVE To observe the inhibitory effect of Gypsophila oldhamiana gypsogenin (GOG) on Lewis lung cancer growth,and to investigate the mechanism of its anti-tumor effect. METHODS A mouse model bearing Lewis lung cancer was used.The 5 experimental groups were divided into a positive control (cis-diaminedichloroplatinum),a negative control,and high,medium and low-GOG dosage groups.The inhibitory action of GOG administration on the lung cancer was observed.After GOG treatment,the lungs were taken out and a lung coefficient computed.The expressions of VEGF,Bcl-2,Bax and P53 in the cancers were determined using immunohistochemical staining. RESULTS The tumor weight of the mice given various doses of GOG was significantly lower compared to the negative-control group (P<0.01),and the lung coefficient of the groups given high and low GOG doses was significantly lower compared to the negative-control group (P<0.01).Immunohistochemical results were shown as follows:i) The VEGF and Bcl-2 expressions in the GOG groups were significantly lower than that of the negative-control group (P<0.05);ii) Bax expression in the groups treated with high and medium GOG doses was significantly higher compared to the negative-control group (P<0.05);iii) The mutant P53 expression in the GOG-treated groups was significantly lower compared to the negative-control group (P<0.05).CONCLUSION GOG can inhibit the growth and metastasis of Lewis lung cancer,and may exert its mechanism of tumor control by inhibition of tumor angiogenesis and induction of apoptosis.

HPLC同时测定丝石竹药材中丝石竹皂苷元和皂皮酸的含量

目的建立高效液相色谱法(HPLC)同时测定丝石竹药材中丝石竹皂苷元和皂皮酸两个齐墩果烷型苷元含量的方法。方法丝石竹药材经酸水解后,采用HPLC测定丝石竹皂苷元和皂皮酸含量。采用Agilent Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-乙腈-0.1%磷酸水溶液(51:9:40)为流动相,流速1.2 m L/min,检测波长210 nm,柱温25℃,进样量10μL。结果丝石竹皂苷元和皂皮酸进样量分别在2.920~17.52μg(r=0.9997)和8.040~48.24μg(r=0.9998)范围内与峰面积呈良好的线性关系。两者平均回收率分别为99.3%(RSD=0.82%)和98.5%(RSD=1.2%)。结论该方法简便、准确、重复性强,可作为同时测定丝石竹药材中丝石竹皂苷元和皂皮酸含量的方法。

霞草苷对人结肠癌HT-29细胞生长和凋亡的影响

目的观察霞草苷对人结肠癌HT-29细胞凋亡的影响及其可能机制。方法用MTT法和流式细胞技术检测浓度为5,10,20,40μmol·L-1霞草苷对HT-29细胞生长、凋亡的影响;用实时荧光定量RT-PCR检测细胞Caspase-3、Bax的mRNA水平。结果霞草苷对HT-29细胞的生长有明显的增殖抑制作用,呈浓度和时间依赖性,细胞凋亡率明显增高。Caspase-3和Bax的mRNA表达量逐渐增加,呈明显的浓度依赖性。结论霞草苷能诱导体外培养的人结肠癌HT-29细胞凋亡,上调Bax表达,活化Caspase-3可能是霞草苷诱导HT-29细胞凋亡的机制之一。