Effect of Qinggan Huayu granule on H22 liver ascitic tumor mice

Objective:To evaluate the therapeutic effect of Qinggan Huayu granule on mice with H22 liver cancer ascites tumor.Methods:A H22 liver cancer ascites mouse model was established by intraperitoneally injecting H22 liver cancer cells.Mice were randomly divided into the model group,the Ganfule group(1.35 g/kg),the fluorouracil group(50 mg/kg i.p),the Qinggan Huayu granule groups at low(0.67 g/kg),medium(1.34 g/kg),and high(2.68 g/kg)doses.Then the mice were administered continuously for 10 days and body weight and abdominal circumference were monitored every 3 days.On day 11,eight rats in each group were randomly selected for dissection to detect the amount of peritoneal water,peritoneal permeability and histopathological changes.The remaining mice were observed for survival.In addition,the vascular endothelial growth factor A(VEGFA)and vascular endothelial growth factor receptor 2(VEGFR2)were determined by Western blotting.Results:Compared with the model group,the weight growth of mice in the fluorouracil group and the medium-dose and high-dose Qinggan Huayu granule groups was slower(P<0.05).Moreover,the abdominal circumference of mice in each treatment group was increased slowly.There were significant differences in abdominal circumference between the fluorouracil group,the medium-dose group and the control group from day 6(P<0.05)while the abdominal circumference of the high dose group was significantly smaller than that of the control group from day 12(P<0.05).Moreover,compared with the model group,the amount of ascites in the medium-and high-dose Qinggan Huayu granule groups was decreased significantly(P<0.05).The optical density value of ascites supernatant in medium-and high-dose Qinggan Huayu granule group and the fluorouracil group decreased significantly(P<0.05)and the survival period of the medium-dose Qinggan Huayu granule group and the fluorouracil group was prolonged prominently(P<0.05).There was no significant difference in the low-dose Qinggan Huayu granule group and the Ganfule group.Peritoneal histopathological assay showed more complete peritoneal structure,less edema and less angiogenesis of the peritoneum in the fluorouracil group and the medium-and high-dose Qinggan Huayu granule group,which was better than that of the Ganfule group and the low-dose group.Compared with the model group,the expressions of VEGFA and VEGFR2 in the medium-dose Qinggan Huayu granule group decreased significantly(P<0.05,P<0.01).Conclusion:Qinggan Huayu granule can inhibit ascites production in the mice model with H22 liver cancer ascites tumor,prolong the survival of mice,and reduce peritoneal permeability and suppress the increase of peritoneal neovascularization.The mechanism may be related to the inhibition of VEGF/VEGFR pathway.

癌痛消胶囊调节小鼠荷H_(22)移植性肝癌细胞VEGF表达的实验研究

目的 :通过观察癌痛消胶囊对小鼠荷H22 移植性肝癌细胞血管内皮细胞生长因子 (vascularendothelialgrowthfactor,VEGF)表达的调节作用 ,探讨该药抑制肿瘤的作用是否与抑制肿瘤血管生成有关。方法 :于昆明种雄性小鼠前肢右腋皮下接种0 2mlH22 肿瘤细胞悬液(浓度为1×107/ml)进行造模。利用造模动物观察瘤块重量 ,计算肿瘤生长抑制率 ,用免疫组化法检测瘤细胞中VEGF的表达程度。结果 :与阴性对照组相比 ,癌痛消胶囊组有明显的抑瘤作用(P<0 05) ,抑瘤率为36 34 % ;病理检测显示 ,造模各组VEGF阳性表达率为100% ,但程度不同 ,癌痛消胶囊组可使肿瘤组织VEGF的表达明显下调 ,与阴性对照组比较 ,差异有显著性意义(P<0 01)。结论 :癌痛消胶囊对小鼠荷H22 移植性肝癌细胞的生长有抑制作用 ,能减少血管生成调控基因VEGF的表达。

青蒿琥酯抗肝癌作用的实验研究

目的 :研究青蒿琥酯的抗肝癌作用。方法 :运用小鼠肝癌H2 2 癌模型、MTT法和集落形成法 ,观察青蒿琥酯对荷瘤鼠和人肝癌SMMC 772 1细胞株的作用。结果 :口服青蒿琥酯 30 0mg·kg-1·d-1,肿瘤受到明显抑制 ,抑瘤率在 3次实验中分别为 49.1% ,48.7% ,46 .6 % ;小鼠生存时间明显延长。青蒿琥酯与 5 氟尿嘧啶有协同抗肿瘤作用。青蒿琥酯体外对人肝癌细胞有明显细胞毒作用 ,其IC50 为 2 .0 7μg·ml-1,同时抑制人肝癌SMMC 772 1克隆原细胞形成集落 ,其IC50 为 2 .48μg·ml-1。结论

黑蚂蚁对H_(22)肝癌小鼠免疫功能的影响

目的 观察黑蚂蚁对H2 2 肝癌小鼠巨噬细胞吞噬功能、淋巴细胞增殖、IL 2水平的影响。方法 将接种H2 2 细胞 2 4h的带瘤小鼠随机分为两组 ,每组 10只。实验组 :将黑蚂蚁烘干研成粉末 ,配成 4 0mg/ml蚁粉液胃饲小鼠 ;对照组 :胃饲等量生理盐水。结果 实验组小鼠巨噬细胞吞噬率、T淋巴细胞增殖及IL 2水平明显增强 ,与对照组比较差异有显著意义 (P <0 0 1)。结论 黑蚂蚁可提高肿瘤等免疫功能低下小鼠的免疫功能。

巨噬细胞过继免疫治疗的实验研究

:目的 研究脂质体瘤苗激活小鼠巨噬细胞的过继免疫治疗作用。方法 制备脂质体 H2 2 肝癌瘤苗 ,脂质体 S1 80 瘤苗 ,Freund's完全佐剂 H2 2 肝癌瘤苗以及对照脂质体瘤苗并免疫小鼠。用 Hanks液清洗免疫鼠的腹腔收集糖原诱导的腹腔巨噬细胞。实验鼠腹腔注射 1× 10 5 个 H2 2 肝癌细胞 2 4小时后再腹腔给予各供体巨噬细胞 5× 10 6个 /只。结果 脂质体 H2 2 瘤苗和 Freund's佐剂 H2 2 瘤苗与对照组相比较 ,其免疫鼠提供的巨噬细胞具有较强的过继免疫治疗作用。结论 脂质体瘤苗激活的小鼠巨噬细胞具有特异性的可过继转移的抗瘤作用。