基于伺服系统H型钢贴标机械手的设计与应用

提出了一种基于西门子V90伺服系统的双轴贴标机械手控制方案,实现了在H型钢生产线中的应用。主要阐述了贴标机械手的网络布局、硬件选型、软件设置、工艺流程等关键功能的实现方法,该贴标机械手在无人干预的情况下可实现高效稳定的运行。

160 km/h市域快线架空刚性接触网系统设计方案研究

架空刚性接触网作为传输列车牵引电能的载体及受电弓滑动取电的机械滑道,是市域快线供电系统的重要组成部分。为保证其能够持续、稳定、可靠地向列车传输电能,文章以国内第一条采用架空刚性接触网授流、速度高达160 km/h的全地下市域快线——广州地铁18号线为例,在分析其架空刚性接触网设计要求的基础上,采用理论计算、有限元仿真等技术手段对架空刚性接触网设计方案进行全方位研究,确定悬挂方式、导高、标准跨距、锚段长度、拉出值布置方案,以及分段绝缘器、膨胀元件及弹性线夹等关键设备的选型要求,并通过仿真及工程实例验证设计方案的有效性,以期为类似工程提供参考和借鉴。

R^(1+1)中Maxwell-Chern-Simons-Higgs模型解的L^(∞)估计

用能量守恒估计和特征线估计的方法解决(1+1)维Maxwell-Chern-Simons-Higgs模型解的Sobolev范数增长性估计问题,给出模型有限能量解的一阶导数的L^(∞)估计,并通过改进指数增长得到解的H^(2)范数的多项式增长.

160 km/h动力集中动车组开行服务范围影响因素及对策研究

研究160 km/h动力集中动车组开行服务范围,对于完善铁路客运产品体系、提高铁路客运经营效益具有重要支撑作用。从购置成本、载客定员、运用条件等方面,分析160 km/h动力集中动车组产品特征,以及在既有普速线路、新建普速线路、200/250 km/h线路上160 km/h动力集中动车组的开行服务范围,探讨160 km/h动力集中动车组开行服务范围的影响因素及对策,与高速铁路形成优势互补格局,实现普速产品优化升级,不断满足人民群众便捷出行需要。

高压输电线路机器人防滑挂线轮毂的H型3D打印装置的研究

针对目前桌面级3D打印机存在打印速度慢、打印精度低的问题,提出并设计了高压输电线路机器人防滑挂线轮毂的H型结构3D打印机。通过对3D打印机结构建模与运动学分析,建立了其数学模型,推导出其逆运动学方程;提出了H型结构3D打印机的控制算法,运用PID控制调节系统的输入信号和输出信号的误差,获得了3D打印机的稳定工作模式。试验结果表明,提出的H型结构的3D打印机的打印精度高于目前常见Prusa I3型结构,能够实现快速平稳打印,打印速度提高20%左右,末端执行器在80 mm/s速度下仍能平稳工作,且运动轨迹理想,应用前景好。

Balmer H_α,H_β and H_γ Spectral Lines Intensities in High-Power RF Hydrogen Plasmas

Hα（Balmer-alpha）, Hβ （Balmer-beta） and Hγ （Balmer-gamma） spectral line inten- sities in atomic hydrogen plasma are investigated by using a high-power RF source. The intensities of the Hα, Hβ and Hγ spectral lines are detected by increasing the input power （0-6 kW） of ICPs （inductively coupled plasmas）. With the increase of net input power, the intensity of Hα im- proves rapidly （0-2 kW）, and then reaches its dynamic equilibrium; the intensities of Hβ can be divided into three processes： obvious increase （0-2 kW）, rapid increase （2-4 kW）, almost constant （4-6 kW）; while the intensities of Hγ increase very slowly. The energy levels of the excited hydro- gen atoms and the splitting energy levels produced by an obvious Stark effect play an important role in the results.

High-speed VUV spectroscopy for edge impurity line emission measurements in HL-2A tokamak

A 20 cm focal length normal incidence vacuum ultraviolet （VUV_20 cm） monochromator with a fast time response has been developed for measuring edge impurity line emission in the wavelength range of 300-2000 A on an HL-2A tokamak. An aberration corrected concave holographic grating with 1200 grooves/mm is adopted in the monochromator, which provides a wavelength dispersion of 40 A mm-1. The aperture is f/4.5. A channel electron multiplier is used as a detector. The time resolution of the system is 17 μs. Wavelength calibration of the system has been done by using a hollow cathode light source in the laboratory with helium and argon gases. The obtained signals of helium and argon spectra are very strong since the inner surface of the monochromator vacuum chamber is blackened and the stray light level is then significantly reduced. The optical property of the system has been examined by scanning the width of the entrance and exit slits. The system is then installed at the mid-port of the HL-2A tokamak and typical line emissions from the HL-2A plasma are measured. Time behaviors of edge impurity line emissions are observed with the fast time response system in different plasma confinement regimes, especially in the H-mode discharges. The result shows that the VUV_20 cm system works very well to measure the edge impurity line emissions in the edge localized modes phase of H-mode discharges.

Balmer-alpha and Balmer-beta Stark line intensity profiles for high-power hydrogen inductively coupled plasmas

We compare Balmer-alpha （Ha） and Balmer-beta （Hβ） emissions from high-power （1.0-6.0 kW） hydrogen inductively coupled plasmas （ICPs）, and propose region Ⅰ （0.0-2.0 kW）, region Ⅱ （2.0-5.0 kW）, and region Ⅲ （5.0-6.0 kW）. In region Ⅰ, both Ha emission intensity （la） and Hβ emission intensity （1β） increase with radio frequency （RF） power, which is explained by the corona model and Boltzmann＇s law, etc. However, in region II, la almost remains constant while 1β rapidly achieves its maximum value. In region Ⅲ, 1α slightly increases with RF power, while 1β decreases with RF power, which deviates significantly from the theoretical explanation for the Ha and Hβ emissions in region I. It is suggested that two strong electric fields are generated in high-power （2.0-6.0 kW） hydrogen ICPs： one is due to the external electric field of high-power RF discharge, and the other one is due to the micro electric field of the ions and electrons around the exited state hydrogen atoms in ICPs. Therefore, the strong Stark effect can play an important role in explaining the experimental results.

ESTABLISHMENT OF A MURINE ASCITES HEPATOMA CELL LINE H_(22)-F_(25)/L AND ITS BIOLOGICAL CHARACTERISTICS

Having been passed for 160 generations, a cell linedesignated as H22-F25/L was established from a murine tumorlymphatic metastatlc model H22-F25 which had been set up in our college. The cell line was in suspension culture with a rapid proliferation and stable growth. The peak tune of cell division and proliferation was 48 and 96 hours after culture. In a week, the cell number was Increased by 25 tunes. H22-F25/L still keeps the features of a poorly differentiated cancer. Its tumor inducing rate (in vivo)was 100% in 615 mice. Lymph node metastasis rate was 50% and pulmonary metastasis rate 10%. H22- F25/ L Is a population of heterogenetlc tumor cells Including 2 stem cell lines (the model number of chromosomes being 43 in 40% tumor cells and 86 in 32%) and some side lines. The common marker chromosomes M1, M2, M3 and M4 were present in all stem and side lines.

Role of the STAT3/survivin signaling pathway in the EML4-ALK-positive lung adenocarcinoma cell line H2228 before and after crizotinib-induced resistance

Objective This study investigated the role of the STAT3/survivin signaling pathway in the EML4-ALK- positive lung adenocarcinoma cell line H2228 before and after crizotinib-induced resistance. The mecha- nism of resistance was studied. Methods Cell viability was determined using the MTT assay. Crizotinib-induced apoptosis in H2228 and H2228 crizotinib-resistant cells treated with the indicated doses of crizotinib was measured at different times （24 h, 48 h, 72 h） using flow cytometry. The levels of p-ALK, ALK, p-STAT3, STAT3, and survivin after treatment of cells with 0, 0.3, and 1 pM crizotinib for 72 h were determined using Western blot analysis. DNA sequencing was used to identify mutations in H2228 crizotinib-resistant cells. Results The crizotinib IC50 values in H2228 and H2228 crizotinib-resistant cells at 72 h were 334.5 nM and 3418 nM, respectively. The resistance index of 1-12228 crizotinib-resistant cells was 10.20. Crizotinib induced apoptosis in H2228 cells and reduced the levels of p-ALK, p-STAT3, and survivin. In contrast, no changes in the levels of p-ALK, p-STAT3, and survivin were observed in H2228 crizotinib-resistant cells. The mutations 2067G--,A and 2182G--,C in EML4-ALK were present in the H2228 crizotinib-resistant cells. Conclusion Crizotinib decreased the viability of H2228 cells in a dose- and time-dependent manner. In the STAT3/survivin pathway, downregulation of p-ALK, p-STAT3, and survivin might contribute to crizo- tinib-induced apoptosis in H2228 ceils. However, the STAT3/survivin pathway in H2228 crizotinib-resistant cells was unaffected by crizotinib treatment. Acquired resistance in H2228 cells might be related to ALK mutations.

Computational Aspects of Electromagnetic Fields near H.V. Transmission Lines

Biological effects of electromagnetic fields on the human body, animals and plants have been a subject of scientific interest and public concern for their risk on the living organisms such as blood leukemia and others. The high voltage transmission and distribution lines, which pass beside some houses, factories and schools are source of electromagnetic fields. This paper presents the field calculations around and near of high voltage transmission lines 220 kV and 500kV. To calculate the induced current, the power density, the electric field and the magnetic field of grounded and ungrounded human body cylindrical model are used. MATLAB program package is used for mathematical calculation of the distribution of the EMF in human body under high voltage power transmission lines.

Line profile studies of hydrodynamical models of cometary compact H II regions

We simulate the evolution of cometary H II regions based on several champagne flow models and bow shock models, and calculate the profiles of the [Ne II] fine-structure line at 12.81 μm, the H30α recombination line and the [Ne III] finestructure line at 15.55 μm for these models at different inclinations of 0°, 30° and 60°. We find that the profiles in the bow shock models are generally different from those in the champagne flow models, but the profiles in the bow shock models with lower stellar velocity （≤ 5 km s^-1） are similar to those in the champagne flow models. In champagne flow models, both the velocity of peak flux and the flux weighted central velocities of all three lines point outward from molecular clouds. In bow shock models, the directions of these velocities depend on the speed of stars. The central velocities of these lines are consistent with the stellar motion in the high stellar speed cases, but they are opposite directions from the stellar motion in the low speed cases. We notice that the line profiles from the slit along the symmetrical axis of the projected 2D image of these models are useful for distinguishing bow shock models from champagne flow models. It is also confirmed by the calculation that the flux weighted central velocity and the line luminosity of the [Ne III] line can be estimated from the [Ne II] line and the H30α line.

不同杂交组合鸡蛋品质的比较分析

试验旨在研究以芦花鸡H系作为亲本的杂交组合蛋品质的差异,为地方鸡种的利用与开发提供理论依据。试验选择萧山鸡(X)、狼山鸡(L)、隐形白羽肉鸡(Y)、来航鸡(H),分别与芦花鸡H系(B)进行正反杂交(F1代),共7个试验组和2个对照组(B、L),每组15个重复。每个组合分别留取同期8 d的受精蛋F2代,孵化后在同一饲养条件和营养水平下饲养至43周龄,每个试验组分别收取30个鸡蛋测定蛋品质。结果显示:各试验组蛋重与对照组B差异不显著(P>0.05);试验组XB蛋黄重极显著高于对照组L(P<0.01);试验组BL、LB蛋黄比率与对照组B、L无显著差异(P>0.05);各试验组及对照组蛋白高度差异不显著(P>0.05);试验组BX、BL、XB、YB蛋壳强度极显著高于对照组L(P<0.01),与对照组B差异不显著(P>0.05);试验组BX、BL、BH、XB、YB蛋壳厚度均极显著高于对照组B和L(P<0.01);蛋黄色泽与蛋重、蛋黄重、蛋黄比率极显著相关(P<0.01),与蛋壳强度显著相关(P<0.05);主成分分析选取5个主成分,特征根累计贡献率为82.852 0%,包含所有杂交组合蛋品质的基本信息。由此可见,芦花鸡H系在与其他地方品种或引进品种杂交时,可较好地保持地方品种蛋品质特性,在选育过程中是优良的亲本素材。

消瘤平移合剂含药血清诱导肝癌H-7402细胞凋亡的实验研究

目的 :观察消瘤平移合剂含药血清对肝癌H 74 0 2细胞凋亡的诱导作用。方法 :运用血清药理学方法 ,选择不同浓度的含药血清与人肝癌H 74 0 2细胞共同孵育 ,于不同时间观察 ,并与空白血清作对照。结果 :消瘤平移合剂含药血清可显著抑制H 74 0 2细胞的增长 ;H 74 0 2细胞在显微镜下呈现典型的凋亡形态学改变 ,流式细胞仪检测可见亚G1峰 ,凋亡呈含药血清浓度、时间依赖性特点 ,对照组及 5 %、10 %含药血清作用 2 4h ,其凋亡率分别为 0 4 9%、5 5 8%、8 4 6% ,4 8h凋亡率分别为 0 83%、11 5 8%、14 2 5 % ;bcl 2基因检测显示bcl 2基因表达受抑。结论 :消瘤平移合剂可通过诱导肿瘤细胞凋亡 ,发挥其临床抗肿瘤、抗转移的作用。

小型高速SWATH船下体型线研究

根据帐篷函数法和切片理论分别编制了SWATH船的阻力、纵向运动理论预报程序 ,理论计算值与试验数据相一致。针对小型高速SWATH船主机置于下体后 ,下体型线的 3种典型变化情况 ,理论分析了下体型线改变对SWATH船的阻力及纵向运动性能的影响 ,并对其做出综合评价。

隐性白H系公鸡体尺性状与精液品质间的典型相关分析

用典型相关分析法对隐性白H系公鸡9个体尺指标与3个精液品质指标进行了相关分析,结果表明:两组性状间的第一典型相关系数为0.8481(p<0.05),占总相关信息的50.5%,体尺性状与精液品质之间的相关关系,主要是由胸围(X8)和精子密度(X11)之间的密切相关所引起,此相关为负相关。建议在隐性白H系公鸡今后的选育中,必须加强对体尺性状的选择,尤其要注重对胸围的选择,要把胸围控制在适当的水平,以达到改善体形结构的同时保证良好的精液品质。

优质油菜新品种(H系)适应性初步研究

研究了7个甘蓝型优质油菜新品种(H系)在安徽地区的适应性,结果表明,优质油菜新品种(H系)H4-4-1、H4-1-5、H4-1-7抗性强,经济性状协调,适应性、丰产性好,具有一定的推广价值。

mCD99L2基因沉默对小鼠B淋巴瘤细胞系A20细胞转化为H/RS样细胞的影响

目的探究mCD99L2基因沉默对小鼠B淋巴瘤细胞系A20细胞转化为H/RS样细胞的影响。方法重组SiRNA表达质粒LV-mCD99L2,体外转染内源性mCD99L2表达阳性的A20细胞,筛选出稳定表达LV质粒的细胞株并扩增培养;采用免疫荧光技术和流式细胞仪检测转化前后两组细胞鼠源CD30表达;透射电镜观察转化后细胞超微结构的形态特点;细胞计数方法动态观测培养细胞干扰组A20-LV-mCD99L2和未经干扰组A20细胞的H/RS样细胞(直径≥25μm)转型率,以人霍奇金淋巴瘤细胞系L428作为对照;采用流式细胞仪检测细胞周期变化。结果获得了稳定表达LV质粒的单克隆细胞株A20-LV-mCD99L2;免疫荧光标记显示转化细胞CD30(+);流式细胞仪检测A20-LV-mCD99L2细胞CD30阳性率为54.4%;透射电镜观察转化后细胞核增大,可见单核、双核及多核,核仁明显的H/RS样细胞;干扰组H/RS样细胞的转型率明显高于未经干扰组(P<0.01)。两组处于S期的细胞无明显差异,两组细胞均未见凋亡峰。结论mCD99L2基因沉默可诱导小鼠B淋巴瘤细胞系A20细胞转化为H/RS样细胞。

DHA复合物对鼠移植瘤细胞和T淋巴细胞细胞周期及凋亡的影响

目的 研究DHA复合物的抗癌机理。方法 用流式细胞术研究了DHA复合物对荷瘤鼠H2 2 细胞和T淋巴细胞细胞周期及凋亡的影响。结果 ( 1)与阴性对照组相比 ,DHA复合物中、高剂量组G0 G1期H2 2 癌细胞百分比明显增加 (P <0 .0 1) ;DHA复合物低、中、高剂量组G0 G1期T细胞百分比明显减小 (P <0 .0 1)。 ( 2 )与阴性对照组相比 ,DHA复合物低、中、高剂量组S期H2 2 癌细胞百分比明显减小 (P <0 .0 1) ,S期T细胞百分比明显增加 (P <0 .0 1)。 ( 3)与阴性对照组相比 ,DHA复合物中剂量组G2 M期H2 2 癌细胞百分比显著减少 (P <0 .0 1) ,DHA复合物低、高剂量组G2 M期H2 2 癌细胞以及DHA复合物低、中、高剂量组G2 M期T细胞百分比均显著升高 (P <0 .0 1)。 ( 4 )与阴性对照组相比 ,DHA复合物低、中、高剂量组H2 2 癌细胞增殖指数 (PI)明显下降 (P <0 .0 1) ,T细胞增殖指数明显升高 (P <0 .0 1)。 ( 5 )与阴性对照组相比 ,DHA复合物低、中、高剂量组H2 2 癌细胞及DHA复合物中、高剂量组T细胞凋亡率明显升高 (P <0 .0 1)。结论 DHA复合物可抑制H2 2 癌细胞的增殖 ,促进T淋巴细胞增殖 ,同时DHA复合物还可促进H2 2 细胞。

抗肿瘤药物与TRAIL联用对喉癌HEP-2细胞株增殖、凋亡的影响

目的探讨化疗药物顺铂(DDP)、5氟尿嘧啶(5-Fu)单独及与TRAIL联合对HEP-2细胞株增殖、凋亡的影响。方法MTT法检测其增殖抑制作用,FCM法检测细胞凋亡率。结果TRAIL与5-Fu、DDP联合作用于HEP-2细胞株时,其抑制率、凋亡率高于单独作用时的抑制率和凋亡率。结论TRAIL与5-Fu、DDP联合作用有协同抑制HEP-2细胞株增殖、诱导HEP-2细胞株凋亡的作用。