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盐胁迫对芨芨草种子萌发苗Na^+,K^+含量与质膜H^+-ATPase活性的影响 被引量:9
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作者 董秋丽 夏方山 董宽虎 《草地学报》 CAS CSCD 北大核心 2010年第6期823-828,共6页
以芨芨草(Achnatherumsplendens)种子萌发苗为试验材料,分别以不同浓度NaCl和Na2SO4进行胁迫,通过对芨芨草叶片和根系中Na+,K+含量以及质膜H+-ATPase活性进行测定,以探讨盐胁迫对芨芨草中Na+,K+分布以及质膜H+-ATPase活性的影响。结果表... 以芨芨草(Achnatherumsplendens)种子萌发苗为试验材料,分别以不同浓度NaCl和Na2SO4进行胁迫,通过对芨芨草叶片和根系中Na+,K+含量以及质膜H+-ATPase活性进行测定,以探讨盐胁迫对芨芨草中Na+,K+分布以及质膜H+-ATPase活性的影响。结果表明:随着NaCl和Na2SO4浓度的增加,芨芨草根系和叶片的Na+含量增加,K+含量下降,K+/Na+比值下降,根系中质膜H+-ATPase活性增加;在NaCl和Na2SO4胁迫下,芨芨草叶片中Na+含量显著低于根系,K+含量显著高于根系,叶片的K+/Na+比值均大于1并明显高于根系,根系的质膜H+-ATPase活性显著高于叶片;与NaCl相比,Na2SO4胁迫下,芨芨草根系向叶片的离子选择性运输系数(TSK,Na)较高,叶片和根系的质膜H+-ATPase活性明显高于相同浓度的NaCl胁迫组。与NaCl胁迫相比,芨芨草对Na2SO4胁迫的适应性更强。 展开更多
关键词 耐盐性 芨芨草 NA^+ K^+ TSK Na 质膜H^+-atpase
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Hp根除后H^+/K^+-ATPase表达水平显著增加 被引量:5
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作者 Osawa H Kita H +1 位作者 Ohnishi H 于丽 《中国处方药》 2006年第5期44-45,共2页
关键词 K^+-atpase 根除后 H^+ HP 胃酸分泌 HP感染 细胞数目 HP根除 分子表达
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Testing the polar auxin transport model with a selective plasma membrane H^(+)-ATPase inhibitor
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作者 Yongqing Yang Xiaohui Liu +10 位作者 Wei Guo Wei Liu Wei Shao Jun Zhao Junhong Li Qing Dong Liang Ma Qun He Yingzhang Li Jianyong Han Xiaoguang Lei 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2022年第6期1229-1245,共17页
Auxin is unique among plant hormones in that its function requires polarized transport across plant cells.A chemiosmotic model was proposed to explain how polar auxin transport is derived by the H^(+)gradient across t... Auxin is unique among plant hormones in that its function requires polarized transport across plant cells.A chemiosmotic model was proposed to explain how polar auxin transport is derived by the H^(+)gradient across the plasma membrane(PM)established by PM H^(+)-adenosine triphosphatases(ATPases).However,a classical genetic approach by mutations in PM H^(+)-ATPase members did not result in the ablation of polar auxin distribution,possibly due to functional redundancy in this gene family.To confirm the crucial role of PM H^(+)-ATPases in the polar auxin transport model,we employed a chemical genetic approach.Through a chemical screen,we identified protonstatin-1(PS-1),a selective small-molecule inhibitor of PM H^(+)-ATPase activity that inhibits auxin transport.Assays with transgenic plants and yeast strains showed that the activity of PM H^(+)-ATPases affects auxin uptake as well as acropetal and basipetal polar auxin transport.We propose that PS-1 can be used as a tool to interrogate the function of PM H^(+)-ATPases.Our results support the chemiosmotic model in which PM H^(+)-ATPase itself plays a fundamental role in polar auxin transport. 展开更多
关键词 ARABIDOPSIS auxin transport inhibitor plasma membrane H^(+)-atpase
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植物跨膜离子转运蛋白与其耐盐性关系研究进展 被引量:11
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作者 王景艳 张高华 +2 位作者 苏乔 安利佳 刘兆普 《西北植物学报》 CAS CSCD 北大核心 2006年第3期635-640,共6页
盐胁迫下植物吸收过多的N a+,使植物体内的离子平衡受到破坏,为了维持其正常生长细胞内的各种离子就必须保持平衡,而这一过程主要是由位于质膜和液泡膜上的离子转运蛋白完成的,并在植物耐盐性方面起关键作用。本文主要对响应盐胁迫的几... 盐胁迫下植物吸收过多的N a+,使植物体内的离子平衡受到破坏,为了维持其正常生长细胞内的各种离子就必须保持平衡,而这一过程主要是由位于质膜和液泡膜上的离子转运蛋白完成的,并在植物耐盐性方面起关键作用。本文主要对响应盐胁迫的几种跨膜转运蛋白如:K+/N a+离子转运蛋白、N a+/H+逆向转运蛋白以及与其相关的H+-ATPase等,在植物耐盐分子生物学方面的研究进展进行综述。 展开更多
关键词 盐胁迫 跨膜离子转运蛋白 K^+/Na^+离子转运蛋白 NA^+/H^+逆向转运蛋白 H^+-atpase 耐盐性
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一株特耐酸酵母R1的分离及其耐酸机理研究 被引量:7
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作者 张国顺 洪青 +2 位作者 刘智 张晓舟 李顺鹏 《微生物学报》 CAS CSCD 北大核心 2004年第5期631-635,共5页
从江西某铜矿的污泥中分离到一株特耐酸酵母菌R1(Rhodotorulasp .) ,能在pH值 1 5~ 6 0的范围内生长 ,最适生长pH为 3 0 ,属于嗜酸菌 ,初步鉴定为红酵母属 (Rhodotorulasp .)。R1在不同培养基中的耐酸能力基本相同。pH冲击实验显示 ,R... 从江西某铜矿的污泥中分离到一株特耐酸酵母菌R1(Rhodotorulasp .) ,能在pH值 1 5~ 6 0的范围内生长 ,最适生长pH为 3 0 ,属于嗜酸菌 ,初步鉴定为红酵母属 (Rhodotorulasp .)。R1在不同培养基中的耐酸能力基本相同。pH冲击实验显示 ,R1通过泵出H+ 吸收K+ 来维持胞内pH的稳定 ,表明膜H+ _ATPase和K+ 在R1耐酸中发挥作用。R1细胞膜的H+ _ATPase活性与R1生长的pH呈负相关。通过PCR克隆了R1的H+ _ATPase基因 (pma) ,并进行了测序 ,序列比较显示该基因的保守性很高。 展开更多
关键词 嗜酸菌 耐酸机理 K^+ H^+-atpase
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应激状态下NO的胃粘膜保护作用及其与壁细胞泌酸的关系 被引量:6
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作者 陆国明 李玉梅 +1 位作者 郭连军 章明 《中国应用生理学杂志》 CAS CSCD 北大核心 2005年第3期301-304,共4页
目的:探讨应激状态下一氧化氮(NO)的胃粘膜保护作用及其与壁细胞泌酸的关系。方法:采用水浸-束缚应激(WRS)方法制备应激性溃疡(SU)动物模型,检测胃粘膜溃疡指数(UI)、胃粘膜NO含量和壁细胞H+,K+-ATPase活性,观察L-硝基精氨酸甲酯(L-NAME... 目的:探讨应激状态下一氧化氮(NO)的胃粘膜保护作用及其与壁细胞泌酸的关系。方法:采用水浸-束缚应激(WRS)方法制备应激性溃疡(SU)动物模型,检测胃粘膜溃疡指数(UI)、胃粘膜NO含量和壁细胞H+,K+-ATPase活性,观察L-硝基精氨酸甲酯(L-NAME)和L-精氨酸(L-Arg)对应激后大鼠壁细胞H+,K+-ATPase活性及胃粘膜损伤的影响。结果:L-NAME(20mg.kg-1)可使胃粘膜NO含量减少(P<0.01),壁细胞H+,K+-AT-Pase活性增加(P<0.05),并加重应激所致的胃粘膜损伤;L-Arg(300mg.kg-1)则使胃粘膜NO含量增加(P<0.01),壁细胞H+,K+-ATPase活性下降(P<0.05),减轻应激所致胃粘膜损伤。结论:NO对应激状态下大鼠胃粘膜具有保护作用,其机制与抑制壁细胞H+,K+-ATPase活性有关。 展开更多
关键词 应激 应激性溃疡 一氧化氮 壁细胞 H^+ K^+-atpase
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Dynamic functional and ultrastructural changes of gastric parietal cells induced by water immersion-restraint stress in rats 被引量:12
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作者 Yu-Mei Li Guo-Ming Lu +3 位作者 Xiao-Ping Zou Zhao-Shen Li Gui-Yong Peng Dian-Chun Fang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第21期3368-3372,共5页
AIM: To investigate the dynamic functional and ultrastructural changes of gastric parietal cells induced by water immersion-restraint stress (WRS) in rats.METHODS: WRS model of Sprague-Dawley (SD) rats was estab... AIM: To investigate the dynamic functional and ultrastructural changes of gastric parietal cells induced by water immersion-restraint stress (WRS) in rats.METHODS: WRS model of Sprague-Dawley (SD) rats was established. Fifty-six male SD rats were randomly divided into control group, stress group and post-stress group. The stress group was divided into 1, 2 and 4 h stress subgroups. The post-stress group was divided into 24, 48 and 72 h subgroups. The pH value of gastric juice, ulcer index (UI) of gastric mucosa and H^+, K^+- ATPase activity of gastric parietal cells were measured. Ultrastructural change of parietal cells was observed under transmission electron microscope (TEM).RESULTS: The pH value of gastric juice decreased time-dependently in stress group and increased in post-stress group. The H^+, K^+-ATPase activity of gastric parietal cells and the UI of gastric mucosa increased time-dependently in stress group and decreased in poststress group. Compared to control group, the pH value decreased remarkably (P = 0.0001), the UI and H^+, K^+- ATPase activity increased significantly (P = 0.0001, P = 0.0174) in 4 h stress subgroup. UI was positively related with stress time (r = 0.9876, P 〈 0.01) but negatively with pH value (r = -0.8724, P 〈 0.05). The parietal cells became active in stress group, especially in 4 h stress subgroup, in which plenty of intracellular canalicular and mitochondria were observed under TEM. In post-stress group, the parietal cells recovered to resting state.CONCOUSION: The acid secretion of parietal cells is consistent with their ultrastructural changes during the development and healing of stress ulcer induced by WRS and the degree of gastric mucosal lesions, suggesting gastric acid play an important role in the development of stress ulcer and is closely related with the recovery of gastric mucosal lesions induced by WRS. 展开更多
关键词 Stress ulcer Gastric parietal cells UItrastructure H^+ K^+-atpase activity Sprague-Dawley rat
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Study of the effects of nesfatin-1 on gastric function in obese rats 被引量:7
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作者 Guo-Tao Yang Hong-Ying Zhao +2 位作者 Yu Kong Ning-Ning Sun Ai-Qin Dong 《World Journal of Gastroenterology》 SCIE CAS 2017年第16期2940-2947,共8页
AIM To investigate the effects of nesfatin-1 on gastric function in obese rats. METHODS The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats wer... AIM To investigate the effects of nesfatin-1 on gastric function in obese rats. METHODS The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes. RESULTS Body weight, body length, and Lee's index of the rats significantly increased in the high-fat dietinduced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. Thisin vivo experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.CONCLUSION Through this research, we provide a theoretical basis for further studies on nesfatin-1, a potential antiobesity drug. 展开更多
关键词 OBESITY NESFATIN-1 H^+/K^+-atpase
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A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity 被引量:6
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作者 BM Srikanta MN Siddaraju Shylaja M Dharmesh 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第39期5196-5207,共12页
AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar... AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti- ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies. RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed. CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management. 展开更多
关键词 Swallow root pectic polysaccharide Gastriculcer H^+ K^+-atpase HISTOPATHOLOGY Antioxidant activity H pylori
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