Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(E.histolytic...Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(E.histolytica)trophozoites in abscessed liver tissues of hamster.Methods:Amoebic liver abscess was experimentally induced in a hamster by injecting 1×10~6of axenically cultured virulent E.histolytica trophozoites(HM1-IMSS strain)into the portal vein.After a week post-inoculation,the hamster was sacrificed and the liver tissue sections were stained with H&E,PAS and IHC stains to delect the amoebic trophozoite.Results:The three stains revealed tissue necrosis and amoebic trophozoites,but with varying clarity.H&E and PAS stained the trophozoites pink and magenta,respectively,however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and morphology.On the other hand,IHC stain revealed distinct brown appearance of the trophozoites in the infected liver tissues.Conclusions:It can be concluded that out of the three stains.IHC is the best for identification of E.histolytica trophozoites in tissue sections.展开更多
Introduction: Immunohistochemistry (IHC) enables the examination of a greater number of trephine biopsy levels and is helpful in determining additional scattered malignant cells. The aim of this study is to detect ext...Introduction: Immunohistochemistry (IHC) enables the examination of a greater number of trephine biopsy levels and is helpful in determining additional scattered malignant cells. The aim of this study is to detect extra-pattern and subtle lymphomatous infiltration in bone marrow biopsies using CD20 and CD3 immunostaining. Patients and Methods: This study was conducted on 100 newly diagnosed Non Hodgkin Lymphoma (NHL) patients. Their bone marrow trephine biopsies were assessed on routine histology [Hematoxylin and Eosin (H & E)], and were further subjected to IHC using CD20 and CD3. Results: Pattern of involvement by H & E was highlighted by IHC. It showed additional interstitial pattern in 9 cases, parasinusoidal streaks in one case and highlighted a patchy pattern in another case with interstitial involvement on H & E. IHC also detected subtle infiltrations on additional 5.5% cases compared with histology alone. It helped in differentiating reactive (12 cases) and malignant lymphoid infiltration (33 cases). Conclusion: CD20 and CD3 immunostaining performed routinely on bone marrow trephine biopsies has the ability to reveal extra-pattern of infiltration and improve detection of subtle lymphoid involvement. A combined procedure identifying several distinctive features, in particular histotopography and IHC, provides a promising way of discriminating reactive from neoplastic lymphoid infiltrates in bone marrow trephine biopsies.展开更多
AIM:To detect whether there is Helicobacter pylori(Hpylori)colonization in the pharynx mucous membraneof healthy people and whether chronic pharyngitis is re-lated to Hpylori infection.METHODS:Fifty cases of chronic p...AIM:To detect whether there is Helicobacter pylori(Hpylori)colonization in the pharynx mucous membraneof healthy people and whether chronic pharyngitis is re-lated to Hpylori infection.METHODS:Fifty cases of chronic pharyngitis refrac-tory over three months were prospectively studied fromMarch 2004 to August 2004 in the otolaryngology outpa-tient department of the Second Hospital of Xi'an JiaotongUniversity.Template-directed dye-terminator incorporat-ed with fluorescence polarization detection(TDI-FP)andmodified Giemsa stain were used to examine pharynxmucous membrane tissue for Hpylori colonization in thepatients with chronic pharyngitis and the healthy peopleas a control group.RESULTS:In the control group,no people were de-tected to have Hpylori in the pharynx.In contrast,in 50cases with chronic pharyngitis,19(38.0%)cases wereH pyIori positive with a TDI-FP assay and 4(8%)caseswere TDI-FP positive with Giemsa staining in the phar-ynx.Sixteen of the 50 pharyngitis cases had stomachailment history,11 cases(68.8%)of these 16 patientswere determined to be H pylori positive in the pharynxwith the TDI-FP assay.x^2 test showed that this infectionrate was remarkably higher(P=0.0007)than that in thecases without stomach ailment history.Giemsa stainingshowed that 3 cases(18.8%)of the patients with stom-ach ailment history were infected with H pylori in the pharynx,which was remarkably higher(P=0.042)thanthat in the patients without stomach ailment history(1case,which was 2.9%).CONCLUSION:H pylori may not be detected in thepharynx of healthy people.Chronic pharyngitis maybe related to H pylori infection.The infection rate withHpylori in the pharynx is higher in patients with stomachailment histories than in patients without stomach ail-ment histories,suggesting that chronic pharyngitis maybe related to stomach ailment history.展开更多
基金Supported by a grant from Universiti Sains Malaysia(grant No.1001/PPSK/813009)received financial support through the USM Fellowship
文摘Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(E.histolytica)trophozoites in abscessed liver tissues of hamster.Methods:Amoebic liver abscess was experimentally induced in a hamster by injecting 1×10~6of axenically cultured virulent E.histolytica trophozoites(HM1-IMSS strain)into the portal vein.After a week post-inoculation,the hamster was sacrificed and the liver tissue sections were stained with H&E,PAS and IHC stains to delect the amoebic trophozoite.Results:The three stains revealed tissue necrosis and amoebic trophozoites,but with varying clarity.H&E and PAS stained the trophozoites pink and magenta,respectively,however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and morphology.On the other hand,IHC stain revealed distinct brown appearance of the trophozoites in the infected liver tissues.Conclusions:It can be concluded that out of the three stains.IHC is the best for identification of E.histolytica trophozoites in tissue sections.
文摘Introduction: Immunohistochemistry (IHC) enables the examination of a greater number of trephine biopsy levels and is helpful in determining additional scattered malignant cells. The aim of this study is to detect extra-pattern and subtle lymphomatous infiltration in bone marrow biopsies using CD20 and CD3 immunostaining. Patients and Methods: This study was conducted on 100 newly diagnosed Non Hodgkin Lymphoma (NHL) patients. Their bone marrow trephine biopsies were assessed on routine histology [Hematoxylin and Eosin (H & E)], and were further subjected to IHC using CD20 and CD3. Results: Pattern of involvement by H & E was highlighted by IHC. It showed additional interstitial pattern in 9 cases, parasinusoidal streaks in one case and highlighted a patchy pattern in another case with interstitial involvement on H & E. IHC also detected subtle infiltrations on additional 5.5% cases compared with histology alone. It helped in differentiating reactive (12 cases) and malignant lymphoid infiltration (33 cases). Conclusion: CD20 and CD3 immunostaining performed routinely on bone marrow trephine biopsies has the ability to reveal extra-pattern of infiltration and improve detection of subtle lymphoid involvement. A combined procedure identifying several distinctive features, in particular histotopography and IHC, provides a promising way of discriminating reactive from neoplastic lymphoid infiltrates in bone marrow trephine biopsies.
基金Supported by a grant from the Bureau of Health in Shaanxi Province,No.2002 02D24 and grants No.NSFC30440080No.NIDCD R21 DC005846
文摘AIM:To detect whether there is Helicobacter pylori(Hpylori)colonization in the pharynx mucous membraneof healthy people and whether chronic pharyngitis is re-lated to Hpylori infection.METHODS:Fifty cases of chronic pharyngitis refrac-tory over three months were prospectively studied fromMarch 2004 to August 2004 in the otolaryngology outpa-tient department of the Second Hospital of Xi'an JiaotongUniversity.Template-directed dye-terminator incorporat-ed with fluorescence polarization detection(TDI-FP)andmodified Giemsa stain were used to examine pharynxmucous membrane tissue for Hpylori colonization in thepatients with chronic pharyngitis and the healthy peopleas a control group.RESULTS:In the control group,no people were de-tected to have Hpylori in the pharynx.In contrast,in 50cases with chronic pharyngitis,19(38.0%)cases wereH pyIori positive with a TDI-FP assay and 4(8%)caseswere TDI-FP positive with Giemsa staining in the phar-ynx.Sixteen of the 50 pharyngitis cases had stomachailment history,11 cases(68.8%)of these 16 patientswere determined to be H pylori positive in the pharynxwith the TDI-FP assay.x^2 test showed that this infectionrate was remarkably higher(P=0.0007)than that in thecases without stomach ailment history.Giemsa stainingshowed that 3 cases(18.8%)of the patients with stom-ach ailment history were infected with H pylori in the pharynx,which was remarkably higher(P=0.042)thanthat in the patients without stomach ailment history(1case,which was 2.9%).CONCLUSION:H pylori may not be detected in thepharynx of healthy people.Chronic pharyngitis maybe related to H pylori infection.The infection rate withHpylori in the pharynx is higher in patients with stomachailment histories than in patients without stomach ail-ment histories,suggesting that chronic pharyngitis maybe related to stomach ailment history.
