主要论述了如何使用Ebbinghaus模型与SWOT商业分析法相融合这一创新思想设计一款英语单词高效记忆工具,分析了记忆工具市场现状以及新软件设计理念和全流程设计思想,针对市场现有相关软件的功能相似性过高而提出创新型优化专属学习方案...主要论述了如何使用Ebbinghaus模型与SWOT商业分析法相融合这一创新思想设计一款英语单词高效记忆工具,分析了记忆工具市场现状以及新软件设计理念和全流程设计思想,针对市场现有相关软件的功能相似性过高而提出创新型优化专属学习方案的新功能点进行阐述,对于“智记”APP进行了功能设计和刨析,并展示了初期UI页面设计构想图,实现并阐述了不同于其他软件的Chart.js和Matplotlib实现的记忆曲线、NoSQL数据库与NumPy相结合的数据处理创新、三种不同的单词抽取新型算法,展示了重点代码实现流程;最后论证了模型优势及实用性和可行性,重点分析了该项目的创新性。It mainly discusses how to use the innovative idea of integrating the Ebbinghaus model and SWOT business analysis method to design an efficient memory tool for English words. It analyzes the current situation of the memory tool market as well as new software design concepts and full-process design ideas, aiming at the existing related software in the market. The functional similarity is too high and the new functional points of the innovative optimized exclusive learning program are elaborated. The functional design and analysis of the “Zhiji” APP is carried out, and the initial UI page design concept diagram is shown, and the different functions are implemented and explained. The memory curve implemented by Chart.js and Matplotlib of other software, the data processing innovation combining NoSQL database and NumPy, and three different new word extraction algorithms demonstrate the key code implementation process;finally, the advantages, practicality and feasibility of the model are demonstrated nature, focusing on analyzing the innovativeness of the project.展开更多
Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report...Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.展开更多
Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生...Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生过敏原Ara h 2的间接双抗夹心化学发光酶免疫分析法,并对该方法的灵敏度、准确度、精密度和特异性进行评价。该方法的检出限为1.085 ng/mL,线性范围为3.12~200 ng/mL,添加回收率为78.30%~94.39%,批内和批间变异系数均小于10%,且特异性良好,与其他常见食物过敏原无交叉反应。该方法与相同抗体所建立的间接双抗夹心酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)方法相比,在灵敏度上表现出一定优势。该研究开发的化学发光酶免疫分析法可对花生食品生产过程中和消费前的Ara h 2过敏原成分检测提供可靠的技术支持。展开更多
文摘主要论述了如何使用Ebbinghaus模型与SWOT商业分析法相融合这一创新思想设计一款英语单词高效记忆工具,分析了记忆工具市场现状以及新软件设计理念和全流程设计思想,针对市场现有相关软件的功能相似性过高而提出创新型优化专属学习方案的新功能点进行阐述,对于“智记”APP进行了功能设计和刨析,并展示了初期UI页面设计构想图,实现并阐述了不同于其他软件的Chart.js和Matplotlib实现的记忆曲线、NoSQL数据库与NumPy相结合的数据处理创新、三种不同的单词抽取新型算法,展示了重点代码实现流程;最后论证了模型优势及实用性和可行性,重点分析了该项目的创新性。It mainly discusses how to use the innovative idea of integrating the Ebbinghaus model and SWOT business analysis method to design an efficient memory tool for English words. It analyzes the current situation of the memory tool market as well as new software design concepts and full-process design ideas, aiming at the existing related software in the market. The functional similarity is too high and the new functional points of the innovative optimized exclusive learning program are elaborated. The functional design and analysis of the “Zhiji” APP is carried out, and the initial UI page design concept diagram is shown, and the different functions are implemented and explained. The memory curve implemented by Chart.js and Matplotlib of other software, the data processing innovation combining NoSQL database and NumPy, and three different new word extraction algorithms demonstrate the key code implementation process;finally, the advantages, practicality and feasibility of the model are demonstrated nature, focusing on analyzing the innovativeness of the project.
基金supported by the National Natural Science Foundation of China,Nos.82171429,81771384a grant from Wuxi Municipal Health Commission,No.1286010241190480(all to YS)。
文摘Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.
文摘Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生过敏原Ara h 2的间接双抗夹心化学发光酶免疫分析法,并对该方法的灵敏度、准确度、精密度和特异性进行评价。该方法的检出限为1.085 ng/mL,线性范围为3.12~200 ng/mL,添加回收率为78.30%~94.39%,批内和批间变异系数均小于10%,且特异性良好,与其他常见食物过敏原无交叉反应。该方法与相同抗体所建立的间接双抗夹心酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)方法相比,在灵敏度上表现出一定优势。该研究开发的化学发光酶免疫分析法可对花生食品生产过程中和消费前的Ara h 2过敏原成分检测提供可靠的技术支持。
