BACKGROUND: There are more than 100 million wa symptomatic HBV carriers (ASCs) in China and they are at a high risk of developing liver disease, which creates a serious health problem. But more than 90% of normal adul...BACKGROUND: There are more than 100 million wa symptomatic HBV carriers (ASCs) in China and they are at a high risk of developing liver disease, which creates a serious health problem. But more than 90% of normal adults clear virus from primary HBV infection, so the difference of gene expression between ASCs and normal adults determines the differential outcomes. To identify differentially expressed genes in ASCs compared to normal adults, we used suppression subtractive hybridization to compare gene expression. METHODS: cDNA subtracted libraries were constructed by suppression subtracted hybridization from peripheral blood monocytes of ASCs and normal adults, the subtracted clones were prescreened by dot blot hybridization, and the levels of genes of interest were confirmed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: One hundred and two and 96 positive clones were acquired from the A-N and N-A libraries, respectively, and 14 and 11 clones were identified by dot blot hybridization in the A-N and N-A libraries. Two genes were confirmed as differentially expressed between a set of ASC and normal adult samples by real-time RT-PCR. CONCLUSIONS: Differentially expressed genes in peripheral blood mononuclear cells between ASCs and normal adults were isolated by suppression subtractive hybridization, and included some new genes. Of the upregulated genes in ASCs, checkpoint suppressor I is associated with DNA damage-induced cell cycle arrest. Perforin I is associated with inflammation. The information about such alterations in gene expression could be useful for elucidating the mechanisms of ASC pathogenesis.展开更多
Acute pancreatitis in pregnancy(APIP) is rare and the reasons for APIP are biliary disease and congenital or acquired hypertriglyceridemia, which could occur during any trimester but more than 50% cases happened durin...Acute pancreatitis in pregnancy(APIP) is rare and the reasons for APIP are biliary disease and congenital or acquired hypertriglyceridemia, which could occur during any trimester but more than 50% cases happened during the third trimester. In this report, one case of a young pregnant woman, a HBV carrier in her 37 th week + 5 d of gestation, was admitted to Emergency Department due to acute abdominal pain, vomiting and diarrhea. The patient was in antiretroviral treatment with telbivudine from 28 weeks of gestation to prevent motherto-child transmission of HBV. Laboratory tests demonstrated hypertriglyceridemia, abdominal computed tomography scan revealed peripancreatic edema. Hyperlipidemic pancreatitits was primary diagnosed and the patient was admitted to the intensive care unit. Considering the possible role in the pathogenesis of pancreatitis, telbivudine was interrupted after birth giving. After supportive treatment, her condition gradually improved. Since it is the first description of APIP during treatment with telbivudine, the association between pregnancy, hyperlipidemia, telbivudine and acute pancreatitis has been well investigated.展开更多
目的:探讨CD4+CD25+Treg细胞在乙型肝炎病毒(hepatitis B virus,HBV)携带者及相关感染状态中分布特征,以期揭示其与HBV携带者的相关性,为临床干预提供依据.方法:计算机检索Pubmed、SCI、Embase、CNKI、万方及维普等数据库,依据Newcastle...目的:探讨CD4+CD25+Treg细胞在乙型肝炎病毒(hepatitis B virus,HBV)携带者及相关感染状态中分布特征,以期揭示其与HBV携带者的相关性,为临床干预提供依据.方法:计算机检索Pubmed、SCI、Embase、CNKI、万方及维普等数据库,依据NewcastleOttawa Scale(NOS)标准评价文献质量,按照PICOS原则提取资料,采用RevMan5.1软件进行Meta分析.结果:纳入文献24篇.HBV携带者外周血Treg细胞含量较健康对照高(P=0.01);与HBV携带者比较,慢性乙型肝炎Treg细胞含量较高(P=0.12),急性乙型肝炎较低(P=0.15);慢性HBV携带者Treg细胞高于非活动性HBsAg携带者(P=0.01).Treg细胞含量与HBV DNA、丙氨酸转氨酶是否存在相关性,因研究一致性差,结论尚不能确定.结论:CD4+CD25+Treg细胞可能成为影响HBV携带者预后及转归的重要因素.展开更多
[目的]观察补肾健脾颗粒对肾虚型E抗原阳性Hepatitis B e-antigen.(HBeAg)阳性慢性乙肝病毒(Hepatitis B Virus,HBV)携带者乙肝病毒脱氧核糖核酸(HBV deoryiboncleicacid,HBV-DNA)、HBeAg及对肝组织病理的影响。[方法]筛选肾虚型HBeAg...[目的]观察补肾健脾颗粒对肾虚型E抗原阳性Hepatitis B e-antigen.(HBeAg)阳性慢性乙肝病毒(Hepatitis B Virus,HBV)携带者乙肝病毒脱氧核糖核酸(HBV deoryiboncleicacid,HBV-DNA)、HBeAg及对肝组织病理的影响。[方法]筛选肾虚型HBeAg阳性慢性HBV携带者60例,随机分为治疗组和对照组两组。治疗组患者给予补肾健脾颗粒治疗,对照组给予安慰剂治疗,共48周。用药前及治疗结束时测定肝功能、乙肝标志物、HBV-DNA、肝脏组织病理等。[结果]治疗结束后,治疗组HBeAg血清学转换率(5.00%)及血清HBV-DNA阴转率(7.50%)显著高于对照组(P<0.05);治疗组治疗前后炎症活动分级及纤维化分期相比显著差异(P<0.01);而对照组在治疗前后无明显差异;临床观察显示补肾健脾颗粒有较好的安全性。[结论]补肾健脾颗粒有利于肾虚型HBeAg阳性慢性HBV携带者HBeAg阴转和HBV-DNA阴转,对肝脏组织炎症活动程度及纤维化分期有改善作用,安全性较好。展开更多
基金supported by a grant from the Science and Technology Department of Zhejiang Province(No.2003C13015)
文摘BACKGROUND: There are more than 100 million wa symptomatic HBV carriers (ASCs) in China and they are at a high risk of developing liver disease, which creates a serious health problem. But more than 90% of normal adults clear virus from primary HBV infection, so the difference of gene expression between ASCs and normal adults determines the differential outcomes. To identify differentially expressed genes in ASCs compared to normal adults, we used suppression subtractive hybridization to compare gene expression. METHODS: cDNA subtracted libraries were constructed by suppression subtracted hybridization from peripheral blood monocytes of ASCs and normal adults, the subtracted clones were prescreened by dot blot hybridization, and the levels of genes of interest were confirmed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: One hundred and two and 96 positive clones were acquired from the A-N and N-A libraries, respectively, and 14 and 11 clones were identified by dot blot hybridization in the A-N and N-A libraries. Two genes were confirmed as differentially expressed between a set of ASC and normal adult samples by real-time RT-PCR. CONCLUSIONS: Differentially expressed genes in peripheral blood mononuclear cells between ASCs and normal adults were isolated by suppression subtractive hybridization, and included some new genes. Of the upregulated genes in ASCs, checkpoint suppressor I is associated with DNA damage-induced cell cycle arrest. Perforin I is associated with inflammation. The information about such alterations in gene expression could be useful for elucidating the mechanisms of ASC pathogenesis.
基金sponsored by the Young Doctor Research Foundation in Beijing Ditan Hospital,Capital Medical University(2011-06)the Basic Research and Clinical Research Collaboration Foundation in Capital Medical University(12JL84)
文摘Acute pancreatitis in pregnancy(APIP) is rare and the reasons for APIP are biliary disease and congenital or acquired hypertriglyceridemia, which could occur during any trimester but more than 50% cases happened during the third trimester. In this report, one case of a young pregnant woman, a HBV carrier in her 37 th week + 5 d of gestation, was admitted to Emergency Department due to acute abdominal pain, vomiting and diarrhea. The patient was in antiretroviral treatment with telbivudine from 28 weeks of gestation to prevent motherto-child transmission of HBV. Laboratory tests demonstrated hypertriglyceridemia, abdominal computed tomography scan revealed peripancreatic edema. Hyperlipidemic pancreatitits was primary diagnosed and the patient was admitted to the intensive care unit. Considering the possible role in the pathogenesis of pancreatitis, telbivudine was interrupted after birth giving. After supportive treatment, her condition gradually improved. Since it is the first description of APIP during treatment with telbivudine, the association between pregnancy, hyperlipidemia, telbivudine and acute pancreatitis has been well investigated.
文摘目的:探讨CD4+CD25+Treg细胞在乙型肝炎病毒(hepatitis B virus,HBV)携带者及相关感染状态中分布特征,以期揭示其与HBV携带者的相关性,为临床干预提供依据.方法:计算机检索Pubmed、SCI、Embase、CNKI、万方及维普等数据库,依据NewcastleOttawa Scale(NOS)标准评价文献质量,按照PICOS原则提取资料,采用RevMan5.1软件进行Meta分析.结果:纳入文献24篇.HBV携带者外周血Treg细胞含量较健康对照高(P=0.01);与HBV携带者比较,慢性乙型肝炎Treg细胞含量较高(P=0.12),急性乙型肝炎较低(P=0.15);慢性HBV携带者Treg细胞高于非活动性HBsAg携带者(P=0.01).Treg细胞含量与HBV DNA、丙氨酸转氨酶是否存在相关性,因研究一致性差,结论尚不能确定.结论:CD4+CD25+Treg细胞可能成为影响HBV携带者预后及转归的重要因素.
文摘[目的]观察补肾健脾颗粒对肾虚型E抗原阳性Hepatitis B e-antigen.(HBeAg)阳性慢性乙肝病毒(Hepatitis B Virus,HBV)携带者乙肝病毒脱氧核糖核酸(HBV deoryiboncleicacid,HBV-DNA)、HBeAg及对肝组织病理的影响。[方法]筛选肾虚型HBeAg阳性慢性HBV携带者60例,随机分为治疗组和对照组两组。治疗组患者给予补肾健脾颗粒治疗,对照组给予安慰剂治疗,共48周。用药前及治疗结束时测定肝功能、乙肝标志物、HBV-DNA、肝脏组织病理等。[结果]治疗结束后,治疗组HBeAg血清学转换率(5.00%)及血清HBV-DNA阴转率(7.50%)显著高于对照组(P<0.05);治疗组治疗前后炎症活动分级及纤维化分期相比显著差异(P<0.01);而对照组在治疗前后无明显差异;临床观察显示补肾健脾颗粒有较好的安全性。[结论]补肾健脾颗粒有利于肾虚型HBeAg阳性慢性HBV携带者HBeAg阴转和HBV-DNA阴转,对肝脏组织炎症活动程度及纤维化分期有改善作用,安全性较好。
