[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavo...[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.展开更多
目的考察槲皮素PLGA-TPGS纳米粒(QPTN)在体内对荷腹水型肝癌高淋巴道转移细胞HCa-F小鼠异位移植实体瘤的治疗效果。方法建立荷HCa-F肝癌细胞小鼠模型后,随机分为阴性对照组、空白纳米粒组、5-氟尿嘧啶溶液(FS)组、槲皮素溶液(QTS)组、...目的考察槲皮素PLGA-TPGS纳米粒(QPTN)在体内对荷腹水型肝癌高淋巴道转移细胞HCa-F小鼠异位移植实体瘤的治疗效果。方法建立荷HCa-F肝癌细胞小鼠模型后,随机分为阴性对照组、空白纳米粒组、5-氟尿嘧啶溶液(FS)组、槲皮素溶液(QTS)组、槲皮素PLGA纳米粒(QPN)组和QPTN组。尾静脉给药,每2 d 1次,连续给药20 d后处死小鼠,剥离肿瘤,称质量,测量肿瘤体积,根据公式计算肿瘤体积增长量和抑瘤率;行HE染色观察肿瘤,全面评价QPTN对荷瘤小鼠的治疗效果。结果小鼠体内给药10次后,QPTN组、QPN组、FS组的肿瘤体积增长量与阴性对照组相比明显减小(P<0.05或P<0.01),QPTN组抑瘤率(59.07%)明显高于QTS组(23.94%)、FS组(35.14%)和QPN组(46.14%)。HE染色结果也显示QPTN组对小鼠肿瘤的治疗效果最明显。结论与QPN、QTS和FS相比,QPTN对荷HCa-F肝癌细胞小鼠异位实体瘤具有较好的治疗效果。展开更多
The E2F family of transcription factors is crucial for cell cycle progression and cell fate decisions.Although E2Fs have been widely studied in mammals,there have been few studies performed in insects.Here,we determin...The E2F family of transcription factors is crucial for cell cycle progression and cell fate decisions.Although E2Fs have been widely studied in mammals,there have been few studies performed in insects.Here,we determined the function of E2F4 in the silkworm,Bombyx mori.We demonstrate that E2F proteins are highly conserved among species from lower animals to higher mammals.Overexpression of the BmE2F4 gene led to cell cycle arrest in the G1 phase,whereas interfering with the BmE2F4 mRNA led to accumulation of cells in the S phase.These results indicate that BmE2F4 is important in cell cycle regulation.We also demonstrate that the BmE2F4 gene is involved in DNA replication of BmN-SWU1 cells and DNA synthesis in the silk gland.Furthermore,we identified a protein called Bm14-3-3ζthat can interact with BmE2F4 and allow it to localize in the nucleus.Overexpression of the Bm14-3-3ζgene led to cell cycle arrest in the G1 phase,while knocking down the gene increased the proportion of cells in S phase.These findings provide important insights into the function of E2F transcription factors and increase our understanding of their involvement in cell cycle regulation.展开更多
基金Supported by Youth Fund Project of Zhaoqing University(QZ202235)Zhaoqing Science and Technology Plan Project(2022040311011).
文摘[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.
文摘目的考察槲皮素PLGA-TPGS纳米粒(QPTN)在体内对荷腹水型肝癌高淋巴道转移细胞HCa-F小鼠异位移植实体瘤的治疗效果。方法建立荷HCa-F肝癌细胞小鼠模型后,随机分为阴性对照组、空白纳米粒组、5-氟尿嘧啶溶液(FS)组、槲皮素溶液(QTS)组、槲皮素PLGA纳米粒(QPN)组和QPTN组。尾静脉给药,每2 d 1次,连续给药20 d后处死小鼠,剥离肿瘤,称质量,测量肿瘤体积,根据公式计算肿瘤体积增长量和抑瘤率;行HE染色观察肿瘤,全面评价QPTN对荷瘤小鼠的治疗效果。结果小鼠体内给药10次后,QPTN组、QPN组、FS组的肿瘤体积增长量与阴性对照组相比明显减小(P<0.05或P<0.01),QPTN组抑瘤率(59.07%)明显高于QTS组(23.94%)、FS组(35.14%)和QPN组(46.14%)。HE染色结果也显示QPTN组对小鼠肿瘤的治疗效果最明显。结论与QPN、QTS和FS相比,QPTN对荷HCa-F肝癌细胞小鼠异位实体瘤具有较好的治疗效果。
基金We thank Dr.Jiangbo Song for phylogenetic analysis.This research was funded by National Natural Science Foundation of China(31872428 and 31872427)Natural Science Foundation of Chongqing(cstc2019jcyj-msxmX0096 and estc 2020jscx-msxmX0045)China Agriculture Research System(CARS-18).
文摘The E2F family of transcription factors is crucial for cell cycle progression and cell fate decisions.Although E2Fs have been widely studied in mammals,there have been few studies performed in insects.Here,we determined the function of E2F4 in the silkworm,Bombyx mori.We demonstrate that E2F proteins are highly conserved among species from lower animals to higher mammals.Overexpression of the BmE2F4 gene led to cell cycle arrest in the G1 phase,whereas interfering with the BmE2F4 mRNA led to accumulation of cells in the S phase.These results indicate that BmE2F4 is important in cell cycle regulation.We also demonstrate that the BmE2F4 gene is involved in DNA replication of BmN-SWU1 cells and DNA synthesis in the silk gland.Furthermore,we identified a protein called Bm14-3-3ζthat can interact with BmE2F4 and allow it to localize in the nucleus.Overexpression of the Bm14-3-3ζgene led to cell cycle arrest in the G1 phase,while knocking down the gene increased the proportion of cells in S phase.These findings provide important insights into the function of E2F transcription factors and increase our understanding of their involvement in cell cycle regulation.
