Upland cotton(Gossypium hirsutum L.)is the most important natural textile fiber crop worldwide.Plant height(PH)is a significant component of plant architecture,strongly influencing crop cultivation patterns,overall yi...Upland cotton(Gossypium hirsutum L.)is the most important natural textile fiber crop worldwide.Plant height(PH)is a significant component of plant architecture,strongly influencing crop cultivation patterns,overall yield,and economic coefficient.However,cotton genes regulating plant height have not been fully identified.Previously,an HD-Zip gene(GhHB12)was isolated and characterized in cotton,which regulates the abiotic and biotic stress responses and the growth and development processes.In this study,we showed that GhHB12 was induced by auxin.Moreover,overexpression of GhHB12 induces the expression of HY5,ATH1,and HAT4,represses the spatial-temporal distribution,polar transport,and signaling of auxin,alters the expression of genes involved in cell wall expansion,and restrains the plant height in cotton.These results suggest a role of GhHB12 in regulating cotton plant height,which could be achieved by affecting the auxin signaling and cell wall expansion.展开更多
同源异型域-亮氨酸拉链(homeodomain-leucine zipper,HD-Zip)蛋白在植物生长发育和适应性抗逆过程中发挥着至关重要的调控作用,而密码子偏好性分析是大麦HD-Zip转录因子家族分子进化及功能研究的重要补充。为探究大麦HD-Zip转录因子家...同源异型域-亮氨酸拉链(homeodomain-leucine zipper,HD-Zip)蛋白在植物生长发育和适应性抗逆过程中发挥着至关重要的调控作用,而密码子偏好性分析是大麦HD-Zip转录因子家族分子进化及功能研究的重要补充。为探究大麦HD-Zip转录因子家族密码子偏好性特征,利用CUSP、CHIPS及CodonW等软件(程序)对32个大麦HD-Zip基因进行了分析。结果显示,大麦HD-Zip家族基因偏好以C或G结尾的密码子,使用频率最高的密码子是CUG,最优密码子为AUC和AGG。该家族基因GC3值分布较分散(0.48~0.98),有62.5%的基因ENC值小于35;GC12与GC3的相关性较强,大多数ENC值频数处于0.03~0.11的范围内,实际ENC值与预期ENC值相近,证明大麦HD-Zip家族基因密码子偏好性较弱,且在进化过程中主要受突变压力的影响。聚类分析表明,基于相对同义密码子使用度的HD-Zip基因聚类与物种进化关系没有必然相关性。对大麦HD-Zip家族代表基因HD-Zip IV 5进行最适受体分析发现,大肠杆菌更适合作为其异源表达受体,遗传转化模式植物中水稻更适合作为该基因的表达受体。本研究为大麦HD-Zip转录因子家族进一步功能研究奠定基础。展开更多
基金supported by the Science and Technology Innovation Program of Hunan Province,China(2020RC2057).
文摘Upland cotton(Gossypium hirsutum L.)is the most important natural textile fiber crop worldwide.Plant height(PH)is a significant component of plant architecture,strongly influencing crop cultivation patterns,overall yield,and economic coefficient.However,cotton genes regulating plant height have not been fully identified.Previously,an HD-Zip gene(GhHB12)was isolated and characterized in cotton,which regulates the abiotic and biotic stress responses and the growth and development processes.In this study,we showed that GhHB12 was induced by auxin.Moreover,overexpression of GhHB12 induces the expression of HY5,ATH1,and HAT4,represses the spatial-temporal distribution,polar transport,and signaling of auxin,alters the expression of genes involved in cell wall expansion,and restrains the plant height in cotton.These results suggest a role of GhHB12 in regulating cotton plant height,which could be achieved by affecting the auxin signaling and cell wall expansion.
文摘同源异型域-亮氨酸拉链(homeodomain-leucine zipper,HD-Zip)蛋白在植物生长发育和适应性抗逆过程中发挥着至关重要的调控作用,而密码子偏好性分析是大麦HD-Zip转录因子家族分子进化及功能研究的重要补充。为探究大麦HD-Zip转录因子家族密码子偏好性特征,利用CUSP、CHIPS及CodonW等软件(程序)对32个大麦HD-Zip基因进行了分析。结果显示,大麦HD-Zip家族基因偏好以C或G结尾的密码子,使用频率最高的密码子是CUG,最优密码子为AUC和AGG。该家族基因GC3值分布较分散(0.48~0.98),有62.5%的基因ENC值小于35;GC12与GC3的相关性较强,大多数ENC值频数处于0.03~0.11的范围内,实际ENC值与预期ENC值相近,证明大麦HD-Zip家族基因密码子偏好性较弱,且在进化过程中主要受突变压力的影响。聚类分析表明,基于相对同义密码子使用度的HD-Zip基因聚类与物种进化关系没有必然相关性。对大麦HD-Zip家族代表基因HD-Zip IV 5进行最适受体分析发现,大肠杆菌更适合作为其异源表达受体,遗传转化模式植物中水稻更适合作为该基因的表达受体。本研究为大麦HD-Zip转录因子家族进一步功能研究奠定基础。
