Human herpesvirus type6,的缩写HHV-6,即人类疱疹病毒6型于1986年首次从淋巴增生疾病患者的血液淋巴细胞中分离获得后,发现健康成年人群中60％～90％均具有它感染的证据,并推测绝大多数患者是在2岁前被感染,该病毒寄居于人类已有长久...Human herpesvirus type6,的缩写HHV-6,即人类疱疹病毒6型于1986年首次从淋巴增生疾病患者的血液淋巴细胞中分离获得后,发现健康成年人群中60％～90％均具有它感染的证据,并推测绝大多数患者是在2岁前被感染,该病毒寄居于人类已有长久的历史。展开更多
Multiple sclerosis is an inflammatory demyelinating disease of the CNS, the ae tiology of which is believed to have both genetic and environmental components. We have investigated one of the candidate viruses for the ...Multiple sclerosis is an inflammatory demyelinating disease of the CNS, the ae tiology of which is believed to have both genetic and environmental components. We have investigated one of the candidate viruses for the environmental componen t of multiple sclerosis, the neurotropic human herpesvirus 6 (HHV-6).Utilizing fluorescent in situ hybridization (FISH) techniques,we have examined human post -mortem tissues for the presence of immediate early and late viral gene express ion in multiple sclerosis patient normal appearing white matter (NAWM), lesional tissue and normal control brain samples. HHV-6 gene transcription was detected in all tissue samples and was restricted to oligodendrocytes, as determined by double mRNA FISH analysis. Quantitative analysis of viral mRNA expression indica ted that both NAWM and lesional multiple sclerosis samples exhibited significant ly higher levels of HHV-6 expression compared with the normal control samples. Lesional samples exhibited the highest levels of viral gene expression,with NAWM exhibiting an intermediate level between lesional and control tissues. Immunofl uorescence against early and late HHV-6 proteins verified active translation of HHV-6 viral mRNA in oligodendrocytes. Southern blot analysis of nested polymer ase chain reactions using extracted genomic DNA and cDNA confirmed the presence of the HHV-6 genome in all individuals,with the active expression profile mirro ring the FISH results. The frequent high level of HHV-6 infection in multiple s clerosis samples suggests a possible role in pathogenesis.展开更多
Three Nanjing local strains (CN5, CN8, CN10) of human herpesvirus 6 (HHV-6] had been isolated and identified by electronmicroscopy, antibody reactions with IFA and specific PCR. The purpose of this paper was to study ...Three Nanjing local strains (CN5, CN8, CN10) of human herpesvirus 6 (HHV-6] had been isolated and identified by electronmicroscopy, antibody reactions with IFA and specific PCR. The purpose of this paper was to study effects on cytokines synthesis and NK activity of peripheral blood mononuclear cells(PBMC) in vitro post infection of human herpesvirus 6. PBMCs cultural supernatants were collected at different hours post infection. Cytokines such as TNF-α, IFN-α and IL-8 could be detected as early as 24 h post infection, plateaued at 48h, and then decreased gradually. The levels of these cytokines in infected PBMCs supernatants were markedly higher than those in uninfected ones, but the IL-6 level was lower than that of uninfected one. These differences between infected and uninfected groups were all significant(P<0.05). There were no differences in the induction of TNF-α, IFN-α and IL-8 between the local strain CN8 and GS strain (all P>0.05), while the inhibition of IL-6 production induced by GS strain was more prominant than that induced by CN8 (P<0.05). It was also found that NK activity was augmented at 24h post infection, which was more striking in CN8 strain infection group than in GS strain infection group (P<0.05), after then, it was gradually decreased. From these reults, it could be inferred that the increase of cytokines synthesis and augmentation of NK activity were associated with convalescence and pathogenicity of the HHV-6 infection. GS strain, which belongs to groups A, disturbed the function of human immunity more remarkably than the local strain CN8, which belongs to group B.展开更多
Background: Since the isolation of HHV-6 in 1986, extensive investigation has revealed it to be ubiquitous and responsible for the majority of cases of a common febrile rash illness of infants known as roseola. Other ...Background: Since the isolation of HHV-6 in 1986, extensive investigation has revealed it to be ubiquitous and responsible for the majority of cases of a common febrile rash illness of infants known as roseola. Other clinical associations including seizure disorders, encephalitis and meningitis have also been stated in various publications. Objective: The aim of the study is to find out if there is any association between HHV-6 infection and the convulsions prevailing at the Child Health Department of the Korle-Bu Teaching Hospital, Accra-Ghana. Methods and Results: Children admitted into the Department of Child Health with episode of convulsions were recruited after informed consent had been sought from subjects. Cerebrospinal fluid (CSF) and Plasma were obtained from patients. PCR directed at the detection of the large tegument protein (LTP) gene in the SIE strain of the HHV-6 in Plasma and CSF from patients was done. The mean age of study subjects was 37.44 months with 53 (64.6%) being males. There was a significant relationship between the convulsions and fever (P < 0.05). Based on CSF characteristics gathered, viral infections may be the probable cause of the observed convulsions but not malaria or bacterial infections. None of the samples from the patients had evidence of HHV-6. Conclusion: The study was unable to establish HHV-6 infection in the CSF and Plasma of patients. What role if any HHV-6 has in convulsions seen in children or neurological diseases at large merits further studies. Other neurotropic viruses need to be investigated as possible causes for the convulsions.展开更多
Clinical manifestations of human herpesvirus 6 (HHV-6) have not been clearly defined, and the role of HHV-6 in human disease among infants and children in Kuwait remains to be fully elucidated. A retrospective study c...Clinical manifestations of human herpesvirus 6 (HHV-6) have not been clearly defined, and the role of HHV-6 in human disease among infants and children in Kuwait remains to be fully elucidated. A retrospective study covering the period between 2008 and 2014 was conducted on infants and children aged from 1 month to 5 years. Blood and CSF samples from infants and children who presented with symptoms suggestive HHV-6 infection were subjected to PCR test for HHV-6. Results showed that 9.3% (n = 42) of infants and children were positive for HHV-6. Fever was the most noticeable symptoms, presenting in 50% (n = 21) of the patients. Also, neutropenia was highly associated with HHV-6 infection, where it presented in 35.8% (n = 15) of infants and children. Our results provided important information about the clinical outcome of HHV-6 infection among infants and children in Kuwait.展开更多
Introduction.Drug hypersensitivity syndrome is a severe life-threatening drug reaction.An association between this syndrome and HHV6 reactivation has been hypothesized.Casereport.A 45 year-old women was treated with s...Introduction.Drug hypersensitivity syndrome is a severe life-threatening drug reaction.An association between this syndrome and HHV6 reactivation has been hypothesized.Casereport.A 45 year-old women was treated with sulfasalazine for polyarthralgia.One month after beginning the treatment,she developed a drug hypersensitivity syndrome with severe acute hepatic failure.HHV6 serology and serum PCR revealed a primary HHV6 infection.Discussion.We report the second case of drug hypersensitivity syndrome associated with a primary HHV6 infection.An immunological disorder may explain such an association by increasing viral replication.Detection and titration of anti-HHV6 antibodies in each case of drug hypersensitivity syndrome should help to confirm this association and possibly modify treatment strategy.展开更多
文摘Multiple sclerosis is an inflammatory demyelinating disease of the CNS, the ae tiology of which is believed to have both genetic and environmental components. We have investigated one of the candidate viruses for the environmental componen t of multiple sclerosis, the neurotropic human herpesvirus 6 (HHV-6).Utilizing fluorescent in situ hybridization (FISH) techniques,we have examined human post -mortem tissues for the presence of immediate early and late viral gene express ion in multiple sclerosis patient normal appearing white matter (NAWM), lesional tissue and normal control brain samples. HHV-6 gene transcription was detected in all tissue samples and was restricted to oligodendrocytes, as determined by double mRNA FISH analysis. Quantitative analysis of viral mRNA expression indica ted that both NAWM and lesional multiple sclerosis samples exhibited significant ly higher levels of HHV-6 expression compared with the normal control samples. Lesional samples exhibited the highest levels of viral gene expression,with NAWM exhibiting an intermediate level between lesional and control tissues. Immunofl uorescence against early and late HHV-6 proteins verified active translation of HHV-6 viral mRNA in oligodendrocytes. Southern blot analysis of nested polymer ase chain reactions using extracted genomic DNA and cDNA confirmed the presence of the HHV-6 genome in all individuals,with the active expression profile mirro ring the FISH results. The frequent high level of HHV-6 infection in multiple s clerosis samples suggests a possible role in pathogenesis.
文摘Three Nanjing local strains (CN5, CN8, CN10) of human herpesvirus 6 (HHV-6] had been isolated and identified by electronmicroscopy, antibody reactions with IFA and specific PCR. The purpose of this paper was to study effects on cytokines synthesis and NK activity of peripheral blood mononuclear cells(PBMC) in vitro post infection of human herpesvirus 6. PBMCs cultural supernatants were collected at different hours post infection. Cytokines such as TNF-α, IFN-α and IL-8 could be detected as early as 24 h post infection, plateaued at 48h, and then decreased gradually. The levels of these cytokines in infected PBMCs supernatants were markedly higher than those in uninfected ones, but the IL-6 level was lower than that of uninfected one. These differences between infected and uninfected groups were all significant(P<0.05). There were no differences in the induction of TNF-α, IFN-α and IL-8 between the local strain CN8 and GS strain (all P>0.05), while the inhibition of IL-6 production induced by GS strain was more prominant than that induced by CN8 (P<0.05). It was also found that NK activity was augmented at 24h post infection, which was more striking in CN8 strain infection group than in GS strain infection group (P<0.05), after then, it was gradually decreased. From these reults, it could be inferred that the increase of cytokines synthesis and augmentation of NK activity were associated with convalescence and pathogenicity of the HHV-6 infection. GS strain, which belongs to groups A, disturbed the function of human immunity more remarkably than the local strain CN8, which belongs to group B.
文摘Background: Since the isolation of HHV-6 in 1986, extensive investigation has revealed it to be ubiquitous and responsible for the majority of cases of a common febrile rash illness of infants known as roseola. Other clinical associations including seizure disorders, encephalitis and meningitis have also been stated in various publications. Objective: The aim of the study is to find out if there is any association between HHV-6 infection and the convulsions prevailing at the Child Health Department of the Korle-Bu Teaching Hospital, Accra-Ghana. Methods and Results: Children admitted into the Department of Child Health with episode of convulsions were recruited after informed consent had been sought from subjects. Cerebrospinal fluid (CSF) and Plasma were obtained from patients. PCR directed at the detection of the large tegument protein (LTP) gene in the SIE strain of the HHV-6 in Plasma and CSF from patients was done. The mean age of study subjects was 37.44 months with 53 (64.6%) being males. There was a significant relationship between the convulsions and fever (P < 0.05). Based on CSF characteristics gathered, viral infections may be the probable cause of the observed convulsions but not malaria or bacterial infections. None of the samples from the patients had evidence of HHV-6. Conclusion: The study was unable to establish HHV-6 infection in the CSF and Plasma of patients. What role if any HHV-6 has in convulsions seen in children or neurological diseases at large merits further studies. Other neurotropic viruses need to be investigated as possible causes for the convulsions.
文摘Clinical manifestations of human herpesvirus 6 (HHV-6) have not been clearly defined, and the role of HHV-6 in human disease among infants and children in Kuwait remains to be fully elucidated. A retrospective study covering the period between 2008 and 2014 was conducted on infants and children aged from 1 month to 5 years. Blood and CSF samples from infants and children who presented with symptoms suggestive HHV-6 infection were subjected to PCR test for HHV-6. Results showed that 9.3% (n = 42) of infants and children were positive for HHV-6. Fever was the most noticeable symptoms, presenting in 50% (n = 21) of the patients. Also, neutropenia was highly associated with HHV-6 infection, where it presented in 35.8% (n = 15) of infants and children. Our results provided important information about the clinical outcome of HHV-6 infection among infants and children in Kuwait.
文摘Introduction.Drug hypersensitivity syndrome is a severe life-threatening drug reaction.An association between this syndrome and HHV6 reactivation has been hypothesized.Casereport.A 45 year-old women was treated with sulfasalazine for polyarthralgia.One month after beginning the treatment,she developed a drug hypersensitivity syndrome with severe acute hepatic failure.HHV6 serology and serum PCR revealed a primary HHV6 infection.Discussion.We report the second case of drug hypersensitivity syndrome associated with a primary HHV6 infection.An immunological disorder may explain such an association by increasing viral replication.Detection and titration of anti-HHV6 antibodies in each case of drug hypersensitivity syndrome should help to confirm this association and possibly modify treatment strategy.
