Plasmid expressing small interfering RNA （siRNA） against HIF-1α （pSilence-2.1-U6-siRNA） was constructed and transfected into LS174T cells in hypoxia condition.After expression of siRNA against HIF-1 α in LS174T ...Plasmid expressing small interfering RNA （siRNA） against HIF-1α （pSilence-2.1-U6-siRNA） was constructed and transfected into LS174T cells in hypoxia condition.After expression of siRNA against HIF-1 α in LS174T cells, expressions of HIF-1 α and N-myc downstream regulated gene 1 （NDRG1） gene were inhibited significantly. HIF-1 cta transcripts were positive in 67.7% （42/62） and 44.4% （8/18） of colorectal adenocarcinoma and adenoma, re- spectively. The mean percentage of cells with positive hybridization of HIF-1 α mRNA increases with the development from Duke stage A to stage C＋D （p〈 0.05）. The positive staining rate of NDRG1 protein was significant higher in than that in colorectal adenoma colorectal adenocarcinoma group group （p〈 0.05）. The level of HIF-1 a transcripts was positively correlated with the level of NDRG1 protein （p 〈 0.05） during colorectal tumor progression. HIF-1α and its down stream gene NDRG1 may play roles in tumor progression of human colorectal carcinoma.展开更多
Objective:To investigate the association between serum HIF-1α,β-catenin levels and the vascular calcification in the rat model of chronic kidney disease(CKD)vascular calcification.Methods:30 SD male rats were random...Objective:To investigate the association between serum HIF-1α,β-catenin levels and the vascular calcification in the rat model of chronic kidney disease(CKD)vascular calcification.Methods:30 SD male rats were randomly divided into normal control group(CON group,n=10)and CKD vascular calcification group(CKD group,n=20).Rats in calcification group were fed with adenine combined with high phosphorus diet.At the end of the 6th week,the blood and urine of the two groups were collected to detect renal function,calcium,phosphorus and 24-hour urinary protein,the renal tissue was stained with HE,the aorta of rats was stained with Von Kossa and calcium content was determined,and the levels of HIF-1α and β-catenin in serum were detected by ElISA method.Results:compared with CON group,24-hour urinary protein,blood BUN,Scr,P,Ca×P,aortic calcium content,serum HIF-1α and β-catenin levels were significantly increased and serum calcium decreased in CKD group(P<0.05);Glomerular atrophy,renal tubule dilatation and interstitial fibrosis were seen in CKD group,Von Kossa staining of calcified nodules in aorta showed more black substance deposition.The levels of serum HIF-1α,β-catenin and serum P,Ca×P were positively correlated with the content of calcium in rat aorta.Conclusion:the levels of serum HIF-1α and β-catenin are significantly increased in patients with vascular calcification in CKD,and they are significantly related to the degree of aortic calcification.展开更多
目的:探讨补血生髓方对肿瘤相关性贫血(cancer related anemia, CRA)小鼠的改善作用及可能的作用机制。方法:C57BL/6雌性小鼠腹腔注射苯肼(50mg/kg),并且皮下接种肺癌LLC细胞建立CRA模型,随机分成模型组、EPO组(1365IU/kg)、补血生髓方...目的:探讨补血生髓方对肿瘤相关性贫血(cancer related anemia, CRA)小鼠的改善作用及可能的作用机制。方法:C57BL/6雌性小鼠腹腔注射苯肼(50mg/kg),并且皮下接种肺癌LLC细胞建立CRA模型,随机分成模型组、EPO组(1365IU/kg)、补血生髓方组(60.67g/kg),每组10只,另取10只正常小鼠作为空白组,给药2周。ELISA检测促红细胞生成素、血清干扰素-γ(interferon-γ,IFN-γ)水平,流式细胞术检测外周血Tregs细胞比例,Real time-PCR及免疫荧光检测缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、程序性死亡-配体1(programmed cell death ligand 1,PD-L1)mRNA及蛋白的表达。结果:与模型组相比,补血生髓方组小鼠的肿瘤体积、肿瘤质量均显著降低(P<0.01)。与空白组相比,模型组血红蛋白、促红细胞生成素、IFN-γ水平显著下调(P<0.01),外周血Tregs细胞比例显著升高(P<0.05)。与模型组相比,补血生髓方组血红蛋白、促红细胞生成素、IFN-γ水平显著上调(P<0.01),外周血Tregs细胞比例显著下降(P<0.01),HIF-1α、PD-L1mRNA和蛋白表达显著降低(P<0.01)。结论:补血生髓方能够改善CRA小鼠的贫血状态,通过下调HIF-1α/PD-L1通路调节CRA小鼠的免疫功能,从而抑制肿瘤的生长。展开更多
基金Supported by the Fund for Key Technologies R and D Pro-gramme of Hubei Province(2006AA301A03 )
文摘Plasmid expressing small interfering RNA （siRNA） against HIF-1α （pSilence-2.1-U6-siRNA） was constructed and transfected into LS174T cells in hypoxia condition.After expression of siRNA against HIF-1 α in LS174T cells, expressions of HIF-1 α and N-myc downstream regulated gene 1 （NDRG1） gene were inhibited significantly. HIF-1 cta transcripts were positive in 67.7% （42/62） and 44.4% （8/18） of colorectal adenocarcinoma and adenoma, re- spectively. The mean percentage of cells with positive hybridization of HIF-1 α mRNA increases with the development from Duke stage A to stage C＋D （p〈 0.05）. The positive staining rate of NDRG1 protein was significant higher in than that in colorectal adenoma colorectal adenocarcinoma group group （p〈 0.05）. The level of HIF-1 a transcripts was positively correlated with the level of NDRG1 protein （p 〈 0.05） during colorectal tumor progression. HIF-1α and its down stream gene NDRG1 may play roles in tumor progression of human colorectal carcinoma.
基金Research project of Sichuan medical association(No.2018SHD2-6)。
文摘Objective:To investigate the association between serum HIF-1α,β-catenin levels and the vascular calcification in the rat model of chronic kidney disease(CKD)vascular calcification.Methods:30 SD male rats were randomly divided into normal control group(CON group,n=10)and CKD vascular calcification group(CKD group,n=20).Rats in calcification group were fed with adenine combined with high phosphorus diet.At the end of the 6th week,the blood and urine of the two groups were collected to detect renal function,calcium,phosphorus and 24-hour urinary protein,the renal tissue was stained with HE,the aorta of rats was stained with Von Kossa and calcium content was determined,and the levels of HIF-1α and β-catenin in serum were detected by ElISA method.Results:compared with CON group,24-hour urinary protein,blood BUN,Scr,P,Ca×P,aortic calcium content,serum HIF-1α and β-catenin levels were significantly increased and serum calcium decreased in CKD group(P<0.05);Glomerular atrophy,renal tubule dilatation and interstitial fibrosis were seen in CKD group,Von Kossa staining of calcified nodules in aorta showed more black substance deposition.The levels of serum HIF-1α,β-catenin and serum P,Ca×P were positively correlated with the content of calcium in rat aorta.Conclusion:the levels of serum HIF-1α and β-catenin are significantly increased in patients with vascular calcification in CKD,and they are significantly related to the degree of aortic calcification.
