The properties of generalized flip Markov chains on connected regular digraphs are discussed.The 1-Flipper operation on Markov chains for undirected graphs is generalized to that for multi-digraphs.The generalized 1-F...The properties of generalized flip Markov chains on connected regular digraphs are discussed.The 1-Flipper operation on Markov chains for undirected graphs is generalized to that for multi-digraphs.The generalized 1-Flipper operation preserves the regularity and weak connectivity of multi-digraphs.The generalized 1-Flipper operation is proved to be symmetric.Moreover,it is presented that a series of random generalized 1-Flipper operations eventually lead to a uniform probability distribution over all connected d-regular multi-digraphs without loops.展开更多
BACKGROUND The diagnostic value of combined methylated branched chain amino acid transaminase 1(BCAT1)/IKAROS family zinc finger 1(IKZF1)in plasma for colorectal cancer(CRC)has been explored since 2015.Recently,severa...BACKGROUND The diagnostic value of combined methylated branched chain amino acid transaminase 1(BCAT1)/IKAROS family zinc finger 1(IKZF1)in plasma for colorectal cancer(CRC)has been explored since 2015.Recently,several related studies have published their results and showed its diagnostic efficacy.AIM To analyze the diagnostic value of methylated BCAT1/IKZF1 in plasma for screening and postoperative follow-up of CRC.METHODS The candidate studies were identified by searching the PubMed,Embase,Cochrane Library,CNKI,and Wanfang databases from May 31,2003 to June 1,2023.Sensitivity,specificity,and diagnostic accuracy were calculated by merging ratios or means.RESULTS Twelve eligible studies were included in the analysis,involving 6561 participants.The sensitivity of methylated BCAT1/IKZF1 in plasma for CRC diagnosis was 60%[95%confidence interval(CI)53-67]and specificity was 92%(95%CI:90-94).The positive and negative likelihood ratios were 8.0(95%CI:5.8-11.0)and 0.43(95%CI:0.36-0.52),respectively.Diagnostic odds ratio was 19(95%CI:11-30)and area under the curve was 0.88(95%CI:0.85-0.91).The sensitivity and specificity for CRC screening were 64%(95%CI:59-69)and 92%(95%CI:91-93),respectively.The sensitivity and specificity for recurrence detection during follow-up were 54%CONCLUSION The detection of methylated BCAT1/IKZF1 in plasma,as a non-invasive detection method of circulating tumor DNA,has potential CRC diagnosis,but the clinical application prospect needs to be further explored.展开更多
The neuronal differentiation of mesenchymal stem cells offers a new strategy for the treatment of neurological disorders.Thus,there is a need to identify a noninvasive and sensitive in vivo imaging approach for real-t...The neuronal differentiation of mesenchymal stem cells offers a new strategy for the treatment of neurological disorders.Thus,there is a need to identify a noninvasive and sensitive in vivo imaging approach for real-time monitoring of transplanted stem cells.Our previous study confirmed that magnetic resonance imaging,with a focus on the ferritin heavy chain 1 reporter gene,could track the proliferation and differentiation of bone marrow mesenchymal stem cells that had been transduced with lentivirus carrying the ferritin heavy chain 1 reporter gene.However,we could not determine whether or when bone marrow mesenchymal stem cells had undergone neuronal differentiation based on changes in the magnetic resonance imaging signal.To solve this problem,we identified a neuron-specific enolase that can be differentially expressed before and after neuronal differentiation in stem cells.In this study,we successfully constructed a lentivirus carrying the neuron-specific enolase promoter and expressing the ferritin heavy chain 1 reporter gene;we used this lentivirus to transduce bone marrow mesenchymal stem cells.Cellular and animal studies showed that the neuron-specific enolase promoter effectively drove the expression of ferritin heavy chain 1 after neuronal differentiation of bone marrow mesenchymal stem cells;this led to intracellular accumulation of iron and corresponding changes in the magnetic resonance imaging signal.In summary,we established an innovative magnetic resonance imaging approach focused on the induction of reporter gene expression by a neuron-specific promoter.This imaging method can be used to noninvasively and sensitively detect neuronal differentiation in stem cells,which may be useful in stem cell-based therapies.展开更多
Two 1D chain metal-organic framework complexes, {Co[（C10H5N3O5）- （H2O）2？H2O]}n （1） and {Cd[（C10H5N3O5）（H2O）2]}n （2）, based on a T-shaped tripodal ligand 4-（4,5-dicarboxy- 1H-imidazol-2-yl） pyridine 1-ox...Two 1D chain metal-organic framework complexes, {Co[（C10H5N3O5）- （H2O）2？H2O]}n （1） and {Cd[（C10H5N3O5）（H2O）2]}n （2）, based on a T-shaped tripodal ligand 4-（4,5-dicarboxy- 1H-imidazol-2-yl） pyridine 1-oxide （H3DCImPyO） have been reacted under hydrothermal conditions, and were characterized by single-crystal X-ray structure analysis, fluorescent analysis and thermal gravimetric analysis. According to single-crystal X-ray determination, complex 1 crystallizes in the monoclinic system, space group P21/n with a = 9.4930（13）, b = 13.2024（18）, c = 10.5203（14） A^°, β = 98.402（2）°, Z = 4, V = 1304.4（3）A^°3; and complex 2 crystallizes in the monoclinic system, space group C2/c with a = 13.557（3）, b = 13.568（3）, c = 6.7804（13） A^°, β = 105.095（2）°, Z = 4 and V = 1204.2（4） A^°3. Fluorescent analysis of complex 2 showed an intense emission band at 466 nm when the exciting radiation was set at 360 nm. Dielectric constants of complexes 1 and 2 were measured at different frequencies with temperature variation.展开更多
基金National Natural Science Foundation of China(No.11671258)。
文摘The properties of generalized flip Markov chains on connected regular digraphs are discussed.The 1-Flipper operation on Markov chains for undirected graphs is generalized to that for multi-digraphs.The generalized 1-Flipper operation preserves the regularity and weak connectivity of multi-digraphs.The generalized 1-Flipper operation is proved to be symmetric.Moreover,it is presented that a series of random generalized 1-Flipper operations eventually lead to a uniform probability distribution over all connected d-regular multi-digraphs without loops.
基金Supported by Natural Science Foundation of Sichuan Province,No.2023NSFSC0729Wu Jieping Foundation Special Fund for Clinical Research,No.320.6750.2022-19-100+1 种基金Foundation of Key Clinical Specialty of Sichuan Province,No.2022School Foundation of Chengdu Medical College,No.CYZYB21-05.
文摘BACKGROUND The diagnostic value of combined methylated branched chain amino acid transaminase 1(BCAT1)/IKAROS family zinc finger 1(IKZF1)in plasma for colorectal cancer(CRC)has been explored since 2015.Recently,several related studies have published their results and showed its diagnostic efficacy.AIM To analyze the diagnostic value of methylated BCAT1/IKZF1 in plasma for screening and postoperative follow-up of CRC.METHODS The candidate studies were identified by searching the PubMed,Embase,Cochrane Library,CNKI,and Wanfang databases from May 31,2003 to June 1,2023.Sensitivity,specificity,and diagnostic accuracy were calculated by merging ratios or means.RESULTS Twelve eligible studies were included in the analysis,involving 6561 participants.The sensitivity of methylated BCAT1/IKZF1 in plasma for CRC diagnosis was 60%[95%confidence interval(CI)53-67]and specificity was 92%(95%CI:90-94).The positive and negative likelihood ratios were 8.0(95%CI:5.8-11.0)and 0.43(95%CI:0.36-0.52),respectively.Diagnostic odds ratio was 19(95%CI:11-30)and area under the curve was 0.88(95%CI:0.85-0.91).The sensitivity and specificity for CRC screening were 64%(95%CI:59-69)and 92%(95%CI:91-93),respectively.The sensitivity and specificity for recurrence detection during follow-up were 54%CONCLUSION The detection of methylated BCAT1/IKZF1 in plasma,as a non-invasive detection method of circulating tumor DNA,has potential CRC diagnosis,but the clinical application prospect needs to be further explored.
基金supported by the National Natural Science Foundation of China,No.81771892(to JHC).
文摘The neuronal differentiation of mesenchymal stem cells offers a new strategy for the treatment of neurological disorders.Thus,there is a need to identify a noninvasive and sensitive in vivo imaging approach for real-time monitoring of transplanted stem cells.Our previous study confirmed that magnetic resonance imaging,with a focus on the ferritin heavy chain 1 reporter gene,could track the proliferation and differentiation of bone marrow mesenchymal stem cells that had been transduced with lentivirus carrying the ferritin heavy chain 1 reporter gene.However,we could not determine whether or when bone marrow mesenchymal stem cells had undergone neuronal differentiation based on changes in the magnetic resonance imaging signal.To solve this problem,we identified a neuron-specific enolase that can be differentially expressed before and after neuronal differentiation in stem cells.In this study,we successfully constructed a lentivirus carrying the neuron-specific enolase promoter and expressing the ferritin heavy chain 1 reporter gene;we used this lentivirus to transduce bone marrow mesenchymal stem cells.Cellular and animal studies showed that the neuron-specific enolase promoter effectively drove the expression of ferritin heavy chain 1 after neuronal differentiation of bone marrow mesenchymal stem cells;this led to intracellular accumulation of iron and corresponding changes in the magnetic resonance imaging signal.In summary,we established an innovative magnetic resonance imaging approach focused on the induction of reporter gene expression by a neuron-specific promoter.This imaging method can be used to noninvasively and sensitively detect neuronal differentiation in stem cells,which may be useful in stem cell-based therapies.
基金Supported by the National Natural Science Foundation of China(No.21201087 and 51502117)NSF of JiangSu Province(BK20131244 and BK20130460)+1 种基金the Foundation of Jiangsu Educational Committee(11KJB150004)the Qinglan Project of Jiangsu Province and Jiangsu Overseas Research&Training Program for University Prominent Young&Middle-aged Teacher and Presidents
文摘Two 1D chain metal-organic framework complexes, {Co[（C10H5N3O5）- （H2O）2？H2O]}n （1） and {Cd[（C10H5N3O5）（H2O）2]}n （2）, based on a T-shaped tripodal ligand 4-（4,5-dicarboxy- 1H-imidazol-2-yl） pyridine 1-oxide （H3DCImPyO） have been reacted under hydrothermal conditions, and were characterized by single-crystal X-ray structure analysis, fluorescent analysis and thermal gravimetric analysis. According to single-crystal X-ray determination, complex 1 crystallizes in the monoclinic system, space group P21/n with a = 9.4930（13）, b = 13.2024（18）, c = 10.5203（14） A^°, β = 98.402（2）°, Z = 4, V = 1304.4（3）A^°3; and complex 2 crystallizes in the monoclinic system, space group C2/c with a = 13.557（3）, b = 13.568（3）, c = 6.7804（13） A^°, β = 105.095（2）°, Z = 4 and V = 1204.2（4） A^°3. Fluorescent analysis of complex 2 showed an intense emission band at 466 nm when the exciting radiation was set at 360 nm. Dielectric constants of complexes 1 and 2 were measured at different frequencies with temperature variation.
