试验对 HMGA1基因的两个 SNPs 位点进行分析,研究了 HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了 HMGA1基因的两个 SNPs 位点（g.-543 T 〉 C 和g.1356 C〉T）,利用 Sequenom 质谱测序...试验对 HMGA1基因的两个 SNPs 位点进行分析,研究了 HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了 HMGA1基因的两个 SNPs 位点（g.-543 T 〉 C 和g.1356 C〉T）,利用 Sequenom 质谱测序平台对杜洛克猪 g.-543 T〉C 和 g.1356 C〉T 位点进行基因分型,并分析该位点多态性与生长、饲料利用性状的关联性。结果发现,杜洛克猪群体 g.-543 T〉C 位点,CT 与 CC 基因型相比,90日龄体重升高了2.15 kg （P 〈0.05）,30 kg 体重日龄降低了3.21 d （P 〈0.05）,断奶重升高,剩余采食量降低,100 kg 体重日龄减少,30-100 kg 平均日增重、40-90 kg 平均日采食量、40-90 kg 平均日增重均降低。杜洛克猪群体 g.1356 C〉T 位点,CC 与 TT 基因型相比,90日龄体重升高了0.37 kg （P 〈0.05）；平均日采食量降低0.13 kg/d （P 〈0.05）,100 kg 背膘厚降低0.43 mm （P 〈0.05）,剩余采食量降低了70.42 g （P 〈0.05）,30 kg 体重日龄降低了0.56 d （P 〈0.05）；40-90 kg 平均日采食量降低了0.17 kg （P 〈0.05）,断奶重升高,100 kg 体重日龄增大,30-100、40-90 kg 平均日增重均降低；CT 与 TT 基因型结果与上结果类似。结果初步表明 HMGA1基因两个 SNPs 位点的突变有利于杜洛克猪的生长、饲料利用效率的提高。展开更多
高迁移率族蛋白A1基因(high mobility group protein A1,HMGA1)是一类对DNA转录起调控作用的基因,广泛存在于多种动物体内。为探究HMGA1基因在绵羊体内的功能,对该基因及其编码产物进行了生物信息学分析。结果显示,绵羊HMGA1基因编码15...高迁移率族蛋白A1基因(high mobility group protein A1,HMGA1)是一类对DNA转录起调控作用的基因,广泛存在于多种动物体内。为探究HMGA1基因在绵羊体内的功能,对该基因及其编码产物进行了生物信息学分析。结果显示,绵羊HMGA1基因编码152个氨基酸残基,其编码的蛋白质分子式为C443H759N151O151S1,分子质量为10.648 35 kDa,等电点pI为10.31。绵羊HMGA1蛋白为不稳定蛋白、亲水性蛋白、非分泌蛋白且无信号肽序列和跨膜结构;亚细胞定位主要存在于细胞核(95.7%)。绵羊与牛的HMGA1蛋白相似度为100%。其二级结构和三级结构主要以无规卷曲组成。展开更多
Porcine carcass traits and organ weights have important economic roles in the swine industry. A total of 576 animals from a Large White×Minzhu intercross population were genotyped using the Illumina PorcineSNP60K...Porcine carcass traits and organ weights have important economic roles in the swine industry. A total of 576 animals from a Large White×Minzhu intercross population were genotyped using the Illumina PorcineSNP60K Beadchip and were phenotyped for 10 traits, speciifcally, backfat thickness （6-7 libs）, carcass length, carcass weight, foot weight, head weight, heart weight, leaf fat weight, liver weight, lung weight and slaughter body weight. The genome-wide association study （GWAS） was assessed by Genome Wide Rapid Association using the mixed model and regression-genomic control approach. A total of 31 single nucleotide polymorphisms （SNPs） （with the most signiifcant SNP being MARC0033464, P value=6.80×10-13） were located in a 9.76-Mb （31.24-41.00 Mb） region on SSC7 and were found to be signiifcantly associated with one or more carcass traits and organ weights. High percentage of phenotypic variance explanation was observed for each trait ranging from 31.21 to 67.42%. Linkage analysis revealed one haplotype block of 495 kb, in which the most signiifcant SNP being MARC0033464 was contained, on SSC7 at complete linkage disequilibrium. Annotation of the pig reference genome suggested 6 genes （GRM4, HMGA1, NUDT3, RPS10, SPDEF and PACSIN1） in this candidate linkage disequilibrium （LD） interval. Functional analysis indicated that the HMGA1 gene presents the prime biological candidate for carcass traits and organ weights in pig, with potential application in breeding programs.展开更多
文摘试验对 HMGA1基因的两个 SNPs 位点进行分析,研究了 HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了 HMGA1基因的两个 SNPs 位点（g.-543 T 〉 C 和g.1356 C〉T）,利用 Sequenom 质谱测序平台对杜洛克猪 g.-543 T〉C 和 g.1356 C〉T 位点进行基因分型,并分析该位点多态性与生长、饲料利用性状的关联性。结果发现,杜洛克猪群体 g.-543 T〉C 位点,CT 与 CC 基因型相比,90日龄体重升高了2.15 kg （P 〈0.05）,30 kg 体重日龄降低了3.21 d （P 〈0.05）,断奶重升高,剩余采食量降低,100 kg 体重日龄减少,30-100 kg 平均日增重、40-90 kg 平均日采食量、40-90 kg 平均日增重均降低。杜洛克猪群体 g.1356 C〉T 位点,CC 与 TT 基因型相比,90日龄体重升高了0.37 kg （P 〈0.05）；平均日采食量降低0.13 kg/d （P 〈0.05）,100 kg 背膘厚降低0.43 mm （P 〈0.05）,剩余采食量降低了70.42 g （P 〈0.05）,30 kg 体重日龄降低了0.56 d （P 〈0.05）；40-90 kg 平均日采食量降低了0.17 kg （P 〈0.05）,断奶重升高,100 kg 体重日龄增大,30-100、40-90 kg 平均日增重均降低；CT 与 TT 基因型结果与上结果类似。结果初步表明 HMGA1基因两个 SNPs 位点的突变有利于杜洛克猪的生长、饲料利用效率的提高。
文摘高迁移率族蛋白A1基因(high mobility group protein A1,HMGA1)是一类对DNA转录起调控作用的基因,广泛存在于多种动物体内。为探究HMGA1基因在绵羊体内的功能,对该基因及其编码产物进行了生物信息学分析。结果显示,绵羊HMGA1基因编码152个氨基酸残基,其编码的蛋白质分子式为C443H759N151O151S1,分子质量为10.648 35 kDa,等电点pI为10.31。绵羊HMGA1蛋白为不稳定蛋白、亲水性蛋白、非分泌蛋白且无信号肽序列和跨膜结构;亚细胞定位主要存在于细胞核(95.7%)。绵羊与牛的HMGA1蛋白相似度为100%。其二级结构和三级结构主要以无规卷曲组成。
基金supported by the Agricultural Science and Technology Innovation Program, China (ASTIPIAS02)the National Key Technology R&D Program of China (2011BAD28B01)+2 种基金the National Natural Science Foundation of China (31201781)the Earmarked Fund for Modern Agroindustry Technology Research System, National Technology Program of China (2011ZX08006-003)the Chinese Academy of Agricultural Sciences Foundation (2011cj-5, 2012ZL069 and 2014ywf-yb-8)
文摘Porcine carcass traits and organ weights have important economic roles in the swine industry. A total of 576 animals from a Large White×Minzhu intercross population were genotyped using the Illumina PorcineSNP60K Beadchip and were phenotyped for 10 traits, speciifcally, backfat thickness （6-7 libs）, carcass length, carcass weight, foot weight, head weight, heart weight, leaf fat weight, liver weight, lung weight and slaughter body weight. The genome-wide association study （GWAS） was assessed by Genome Wide Rapid Association using the mixed model and regression-genomic control approach. A total of 31 single nucleotide polymorphisms （SNPs） （with the most signiifcant SNP being MARC0033464, P value=6.80×10-13） were located in a 9.76-Mb （31.24-41.00 Mb） region on SSC7 and were found to be signiifcantly associated with one or more carcass traits and organ weights. High percentage of phenotypic variance explanation was observed for each trait ranging from 31.21 to 67.42%. Linkage analysis revealed one haplotype block of 495 kb, in which the most signiifcant SNP being MARC0033464 was contained, on SSC7 at complete linkage disequilibrium. Annotation of the pig reference genome suggested 6 genes （GRM4, HMGA1, NUDT3, RPS10, SPDEF and PACSIN1） in this candidate linkage disequilibrium （LD） interval. Functional analysis indicated that the HMGA1 gene presents the prime biological candidate for carcass traits and organ weights in pig, with potential application in breeding programs.
