目的研究HOXA13基因与喉癌的关系及临床应用价值。方法利用The Cancer Genome Atlas(TCGA)数据库分析HOXA13在喉癌中的表达及与喉癌患者的生存关系和诊断价值。采用荧光定量PCR检测45例喉癌组织及癌旁组织样本中HOXA13表达水平。应用Gen...目的研究HOXA13基因与喉癌的关系及临床应用价值。方法利用The Cancer Genome Atlas(TCGA)数据库分析HOXA13在喉癌中的表达及与喉癌患者的生存关系和诊断价值。采用荧光定量PCR检测45例喉癌组织及癌旁组织样本中HOXA13表达水平。应用Gene Set Enrichment Analysis(GSEA)软件探索HOXA13影响喉癌进展潜在信号通路。结果TCGA数据库和验证集均证实喉癌组织样本中HOXA13表达水平均显著高于癌旁组织(均P>0.05);HOXA13表达水平与喉癌各项临床因素无明显相关性,但与喉癌患者低总体生存率相关;多因素Cox分析显示HOXA13表达水平能够成为喉癌预后的独立影响因素;ROC诊断分析提示在TCGA数据集中HOXA13诊断喉癌的曲线下面积(AUC)为0.906,敏感性和特异性分别为78.4%和100%。而在验证集中AUC为0.898敏感性和特异性分别为80%和86.7%。GSEA软件分析发现HOXA13基因可通过多种信号通路,如WNT信号通路,促进喉癌发生。结论HOXA13基因在喉癌发生发展过程中发挥重要作用,可作为一个潜在的喉癌分子诊断标志物并且是喉癌不良预后的独立影响因素。展开更多
The occurrence and development of digestive tract tumors are mainly caused by the interaction of genetic and environmental factors, multiple incentives, multiple genes involved, and multi-step regulation. With the dev...The occurrence and development of digestive tract tumors are mainly caused by the interaction of genetic and environmental factors, multiple incentives, multiple genes involved, and multi-step regulation. With the development of gene sequencing technology, precise treatment of tumor era has arrived. Studies have shown that the HOXA13 gene in the homeobox gene is abnormally expressed in digestive system tumors. Studies have shown that HOXA13 may have a certain relationship with the occurrence, development and prognosis of the tumor, pending the diagnosis and treatment of digestive tract tumors with a new gene target.展开更多
文摘目的研究HOXA13基因与喉癌的关系及临床应用价值。方法利用The Cancer Genome Atlas(TCGA)数据库分析HOXA13在喉癌中的表达及与喉癌患者的生存关系和诊断价值。采用荧光定量PCR检测45例喉癌组织及癌旁组织样本中HOXA13表达水平。应用Gene Set Enrichment Analysis(GSEA)软件探索HOXA13影响喉癌进展潜在信号通路。结果TCGA数据库和验证集均证实喉癌组织样本中HOXA13表达水平均显著高于癌旁组织(均P>0.05);HOXA13表达水平与喉癌各项临床因素无明显相关性,但与喉癌患者低总体生存率相关;多因素Cox分析显示HOXA13表达水平能够成为喉癌预后的独立影响因素;ROC诊断分析提示在TCGA数据集中HOXA13诊断喉癌的曲线下面积(AUC)为0.906,敏感性和特异性分别为78.4%和100%。而在验证集中AUC为0.898敏感性和特异性分别为80%和86.7%。GSEA软件分析发现HOXA13基因可通过多种信号通路,如WNT信号通路,促进喉癌发生。结论HOXA13基因在喉癌发生发展过程中发挥重要作用,可作为一个潜在的喉癌分子诊断标志物并且是喉癌不良预后的独立影响因素。
文摘The occurrence and development of digestive tract tumors are mainly caused by the interaction of genetic and environmental factors, multiple incentives, multiple genes involved, and multi-step regulation. With the development of gene sequencing technology, precise treatment of tumor era has arrived. Studies have shown that the HOXA13 gene in the homeobox gene is abnormally expressed in digestive system tumors. Studies have shown that HOXA13 may have a certain relationship with the occurrence, development and prognosis of the tumor, pending the diagnosis and treatment of digestive tract tumors with a new gene target.
