HPTLC联用像素与光密度法快速定量茶饮料中的EGCG

以HPTLC分析平台,分别用像素分析法和光密度法,建立并对比两种快速检测茶饮料中EGCG含量的方法。以3种市售茶饮料为代表性样品,对比两种方法的应用性能,评估像素分析替代仪器依赖的光密度检测作为茶饮料中EGCG含量检测的新方法的先进性。结果显示,HPTLC-像素分析方法、HPTLC-光密度方法检测的定量限分别为6.8,5 mg/kg,均可以定量茶饮料中EGCG。两种方法目标物在200~1200 ng/斑点浓度范围内线性相关系数相差不大,均在0.999以上。两种方法的回收率均满足要求,HPTLC-像素分析方法测定3种样品加标回收率分别在102.4%~110.8%,102.3%~118.8%,100.3%~113.5%之间,HPTLC-光密度方法测定3种样品加标回收率分别在:103.5%~110.8%,95.3%~111.2%,99.3%~111.2%之间,表明两种方法具有良好的准确性。两种方法的精密度也相差不大,HPTLC-像素分析方法RSD<6.7%,HPTLC-光密度方法RSD<3.2%。结论:HPTLC检测技术中,简单易用的像素分析可以较好地代替昂贵的仪器化光密度扫描,提高方法的性价比和实用性。

野葛根中葛根素的提取及HPTLC测定

采用有机溶剂萃取结合酸水解的方法 ,直接从野葛根中分离葛根素 ,并用 HPTL C进行纯度分析。产率为1.15 % ,含量为 97.6 %。操作简便 ,产品纯度和产率高 。

芹菜提取物中芹菜素的HPLC与HPTLC定量分析研究

通过采用高效液相色谱(HPLC)、紫外光谱和黄酮颜色特征反应对芹菜提取物主要成分芹菜素进行定性鉴定,同时建立定量测量芹菜素的高效薄层色谱(HPTLC)方法。结果表明:芹菜提取物的主要成分是黄酮醇-芹菜素,与芹菜素标准品具有相同的光谱和色谱性质;采用G60高效硅胶板,以氯仿-甲醇-水(18:2.3:0.35)为展开剂,芹菜素标样与试样有相同的Rf值为0.65;用最小二乘法作线性回归,芹菜素含量与斑点峰面积的标准曲线回归方程:y=15138x+7594.8,R2=0.993,线性范围:0.332～0.996μg/斑点。精密度实验RSD(n=5)=1.33%,平均回收率97.82%。

3种五味子药材HPTLC鉴别与HPLC含量测定

目的建立五味子HPTLC定性鉴别和HPLC含量测定方法。方法以五味子甲素、五味子乙素、五味子醇甲、五味子酯甲为对照品,采用HPTLC法带状点样于GF254板上,以石油醚(30～60℃)-甲酸乙酯-甲酸(15∶5∶1)为展开剂,上行展开8 cm,置于紫外灯254nm下观察,比较不同来源的五味子活性成分的差异。以五味子醇甲为对照品,采用HPLC法,ZORBAX SB-C18(4.6 mm×250 mm,5μm),流动相:甲醇-水(65∶35);流速:1.0 ml.min-1;柱温:室温;检测波长:254 nm,比较不同来源的五味子醇甲的含量。结果 HPTLC法能够准确快速鉴别不同来源的五味子;3种药材中的五味子醇甲含量有差异。结论该方法简便、准确、重现性好,可从定性、定量两方面评价五味子药材质量。

HPTLC法测定芹菜提取物中芹菜素的含量

本研究建立了测定芹菜素的高效薄层色谱（HPTLC）法。采用CAMAG全自动薄层色谱仪，高效硅胶G60薄层板，展开剂为氯仿-甲醇-水（18：2．3：0．35），测定波长为345nm，测定了芹菜提取物中芹菜素含量，在0．116～0．696μg／ml范围内呈良好的线性关系（R^2〉0．99）。该方法简单、快速，分离度好，灵敏度高，适用于芹菜提取物中芹菜素的测定。

农药土壤光解与HPTLC技术的应用

农药土壤光解是化学农药在土壤环境中消失的重要途径。土壤的非匀质相分布和吸附态分子的特征给农药土壤光解的研究带来很多困难,多年来对农药土壤光解并没有形成一种成熟的方法。HPTLC技术在研究农药吸附态光解上有很多优点,是一种很好的研究土壤中农药吸附态光化学行为的实验室方法。

银杏聚戊烯醇同系物的提取及其HPTLC法的初步分析

目的 :确定银杏聚戊烯醇各同系物的链长。方法 :从银杏叶的石油醚提取物中分离获得了纯度较高的聚戊烯醇同系物的混合物。进而利用反相高效薄层色谱 ,以丙酮为展开剂 ,碘蒸气显色后呈显五个斑点。结果 :经与标准品对照 ,可确定为聚戊烯醇 16～聚戊烯醇 2 0五个单体。结论 :用HPTLC能快速、简便地确定各同系物链长 ,较HPLC方法费用低廉。

甘薯长喙壳菌毒素的HPTLC实验方法研究

在 3 0℃条件下在石油醚 -醋酸乙酯 (2 :1,v/ v)和苯 -甲醇 (9:1,v/ v)两种溶剂系统中 ,对甘薯酮 (C)、甘薯酮醇 (A)等毒素有较好的分离效果 ,采用高效薄层色谱法检测甘薯酮 ,最低检测限量纯品为 0 .0 0 2μg,粗品为 0 .0 1μg。

复方补乌糖浆的HPTLC图谱研究

目的研究复方补乌糖浆(补骨脂、川芎、当归、生首乌等)高效薄层色谱,用于质量控制。方法复方补乌糖浆用碳酸钠溶液溶解,乙酸乙酯和正丁醇萃取,不同极性部位进行特征图谱研究,用Chromafinger软件进行相似度分析。结果对10批复方补乌糖浆进行了考察,乙酸乙酯部位确定了12个共有峰,各批样品特征图谱相似度均大于0.99;正丁醇部位确定了10个共有峰,各批样品特征图谱相似度均大于0.97,共有峰中包括补骨脂素、异补骨脂素、大黄素、丹参酮ⅡA、丹酚酸B和阿魏酸等6个色谱峰。结论此方法未发现复方补乌糖浆中左旋紫草素、齐墩果酸和藁本内酯。

HPTLC-DPPH法定性定量分析保健品中芦丁

将1,1-二苯基-2-三硝基苯肼(DPPH)还原变色反应与高效薄层色谱分离相结合,建立了一种HPTLC-DPPH联用测定保健品中芦丁的方法,并考察了DPPH显色反应时间、流动相配比、光密度扫描参数对色谱分离、扫描效果的影响,同时测试了该方法的回收率和精密度。结果显示,该方法的标准添加回收率为82%～97%,精密度RSD<10%,且可消除保健品中复杂基质的干扰,适用于保健品中芦丁的快速测定。

HPTLC法测定绿茶脂溶性色素

采用丙酮∶石油醚(体积比3∶2)混合提取液直接浸提绿茶中的脂溶性色素,用石油醚∶丙酮(体积比3∶1)溶剂系统层析展开,使用CAMAG高效薄层扫描仪在450和700 nm双波长扫描脂溶性色素,结果表明,直接浸提脂溶性色素方法简便,稳定;丙酮与石油醚混合的溶剂展开系统分离清晰色素斑点13个,根据其最大吸收峰波长和Rf值确定各斑点分别为β-胡萝卜素,γ-胡萝卜素,脱镁叶绿素酸酯a,b,脱镁叶绿素a,b,玉米黄素,紫黄质,叶绿素酸酯a,叶绿素a,叶绿素酸酯b,叶绿素b.

HPTLC法测定庆大霉素组分及其相关物质

目的:建立测定庆大霉素组分及其相关物质的 HPTLC 法。方法:用高效薄层色谱法测定庆大霉素组分及其相关物质。CAMAG 全自动薄层色谱仪;薄层板采用 MEREK 高效硅胶 G 板,展开剂为氯仿-甲醇-25%氨水(5:7:6),测定波长为485nm。结果:庆大霉素在3.98～39.8μg·mL^(-1)。范围内各组分呈良好的线性关系(r>0.99);检测限为 ng 级。庆大霉素注射液中组分及其相关小组分之间分离度良好。结论:该方法简单、快速,灵敏度高,适用于庆大霉素组分中相关物质的分离测定。

HPTLC-DPPH显色-像素分析快速测定酚类抗氧化剂:以食用油和烘焙食品中TBHQ为例

目前,酚类抗氧化剂TBHQ的常用检测方法存在前处理烦琐、耗时长、单次只能测定一个样品的问题,因此亟需建立一种高通量、快速准确的检测方法。为此,将高效薄层色谱(HPTLC)分离与1,1-二苯基-2-三硝基苯肼(DPPH)还原变色反应、图像像素定量分析相结合,建立了一种快速筛检酚类抗氧化剂TBHQ的方法。在此基础上,以食用油、面包和蛋糕为代表性样品,验证所建方法的实际应用性能。结果显示:所建方法检测TBHQ的检测限为70 ng/zone (35 mg/kg),定量限为140 ng/zone (70 mg/kg),定量限低于GB 2760—2014规定的使用限量(200 mg/kg);通过对比分析,样品提取物中共存的背景基质对目标物定量的干扰可以忽略,证明了该方法具有良好的选择性;目标物在150~550 ng/zone范围内表现出良好的线性(R^(2)=0.989 2);多次平行(n=3)测试和国标规定使用限量水平附近的加标回收率测试结果证明了该方法具有较好的精密度和准确度。综上,该方法具有基质耐受性强、定量准确、简便可靠且分析通量大等优点,特别适合粮油食品中各种抗氧化剂的快速定量筛查,具有凝炼成为相关领域国家标准的潜力和价值。

Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique

Objective:To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata(A.lanata) L.Methods:Preliminary phytochemicul screening was done by the method as Harborne described.HPTLC studies were canied out as Harborne and Wagner et al described.The Ethyl acetate-ethanol-water(8:2:1.2) was employed as mobile phase for glycosides.Results:The desired aim was achieved using Chloroform-acetone(8:2) as the mobile phase.The methanolic extract of stem,leaves,root,flower and seeds of A.lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number(11) of steroids has been observed in leaves followed by root(10).Conclusions: HPTLC profile of steroids has been chosen here to reveal the diversity existing in A.lanata.Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.

Quantification of total phenol,flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits

Objective:To carry out the physicochemical and phytochemical standardization with high performance thin layer chromatography fingerprinting of Piper nigrum L.(P.nigrum)fruits in order to ascertain the standard pharmacognostical parameters of this king of spices.Methods:Many standardization parameters like extractive values,total ash value,water soluble ash value and acid insoluble ash,moisture content,loss on drying and pH values of P.nigrum L.fruits were analyzed.The method of Harborne was adopted for the preliminary phytochemicals screening.Analysis of total phenolic and flavonoid contents,pesticides residues,aflatoxin and heavy metals were also performed.CAMAG-high performance thin layer chromatography system was used for fingerprinting of methanolic extract of P.nigrum L.fruits.Results:The results of phytochemicals testing indicated the presence of carbohydrates,phenolic compounds,flavonoids,alkaloids,proteins,saponins,lipids,sterols and tannins in various solvent extracts.Total phenolic and flavonoid contents in methanolic extract were found to be 1.728 1 mg/g and 1.087 ug/g,respectively.Heavy metals concentrations were found to be within standard limits.Aflatoxins and pesticides residues were absent.Conclusions:The outcome of this study might prove beneficial in herbal industries for identification,purification and standardization of P.nigrum L.fruits.

Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements,teas and commercial creams

Objective:To develop and validate a simple,accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol inZingiber officinaleextract and gingercontaining dietary supplements,teas and commercial creams.Methods:The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254(E-Merck,Germany)with n-hexane:ethyl acetate 60:40(v/v)as mobile phase.Camag TLC Scanner III was used for the UV densitometric scanning at 569.Results:This system was found to give a compact spot of 8-gingerol at retention factor(Rf) value of(0.39依0.04)and linearity was found in the ranges 50-500 ng/spot(r2=0.9987).Limit of detection(12.76 ng/spot),limit of quantification(26.32 ng/spot),accuracy(less than 2%)and recovery(ranging from 98.22-99.20)were found satisfactory.Conclusions:The HPTLC method developed for quantification of 8-gingerol was found to be simple,accurate,reproducible,sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinaleextract and ginger-containing dietary supplements,teas and commercial creams.

Free radical scavenging potential and HPTLC analysis of Indigofera tinctoria linn(Fabaceae)

The objective of this study was to evaluate the free radical scavenging potential and high performance thin layer chromatography （HPTLC） fingerprinting of Indigofem tinctoria （I. tinctoria）, Phytochemical analysis was carried out using standard methods, and free radical scavenging activity of the plant was determined using 2,2-diphenyl-l-picrylhydrazy （DPPH）, nitric oxide （NO） and superoxide anion （O2-） radical scavenging capacities. HPTLC plate was kept in CAMAG TLC Scanner 3 and the Rf values at fin- gerprint data were recorded by WlNCATS software, Aqueous extract of I. tinctoria reliably showed the total phenolics （267.2 ± 2.42 mg/g）, flavonoids （75.43 ± 3.36 mg/g） and antioxidants （349.11 ±8.04 mg/g）. The extract was found to have DPPH （52.08%）, NO （23.12%） and 02 （26.79%） scavenging activities at the concentration of 250 pg/mL and the results were statistically significant compared with ascorbic acid standard （p 〈 0.05）. HPTLC results confirmed that the extract contained several potential active com- ponents such as phenols, flavonoids, saponins and terpenoids as the slides revealed multi-colored bands of varying intensities. This study confirmed that the plant had multipotential antioxidant and free ra- dicals scavenging activities.

Secondary metabolite credentials ofEvolvulus alsinoides by high performance thin layer chromatography(HPTLC)

Plants and plant-based products are the bases of many modern pharmaceuticals that are current in use today for various diseases.The aim of the study was to investigate the biochemical constituents and high performance thin layer chromatography（HPTLC） finger printing of the ethanolic extract of Evolvulus alsinoides.Phytochemical screening was done by standard procedures and HPTLC method was also established to analyze alkaloids,flavonoids and phenolic compounds from the ethanolic extract of Evolvulus alsinoides.Preliminary phytochemical screening showed that ethanol extracted more secondary metabolites than other solvents.HPTLC fingerprinting analysis showed the presence of various alkaloids,flavonoids and phenols（quercetin） in the ethanolic extract.It can be concluded that Evolvulus alsinoides may serve as a source of potent antioxidants that may be used in the prevention of various diseases such as cancer,diabetes and cardiovascular diseases due to the presence of phenolic compounds.HPTLC finger print of Evolvulus alsinoides may be useful in the differentiation of the species from adulterants and act as a biochemical marker for this medicinally important plant in the pharmaceutical industry and plant systematic studies.