Hrr25是酿酒酵母中具有丝氨酸/苏氨酸蛋白激酶活性的I型酪蛋白激酶家族成员.在自噬过程中Hrr25可以磷酸化多种蛋白调控自噬过程,然而目前对Hrr25的作用底物分析仍不明确,存在巨大的科研探究价值.Hrr25相互作用蛋白的鉴定可以通过免疫沉...Hrr25是酿酒酵母中具有丝氨酸/苏氨酸蛋白激酶活性的I型酪蛋白激酶家族成员.在自噬过程中Hrr25可以磷酸化多种蛋白调控自噬过程,然而目前对Hrr25的作用底物分析仍不明确,存在巨大的科研探究价值.Hrr25相互作用蛋白的鉴定可以通过免疫沉淀结合质谱分析来实现.构建了表达Hrr2513Myc蛋白的菌株,以Hrr2513Myc为靶蛋白进行免疫沉淀,将免疫沉淀的样品进行质谱检测.利用Venny2.1软件,得到在诱导自噬条件下与Hrr25特异性相互作用的蛋白.通过DAVID数据库进行基因本体论(gene ontology,GO)和京都基因和基因组百科全书(kyoto encyclopedia of genes and genome,KEGG)基因富集.结果在诱导自噬条件下筛选出321种特异与Hrr25相互作用的蛋白,根据P<0.05,GO富集共得到23项生物过程(biological process,BP)、16项细胞组成(cellular component,CC)、21项分子功能(molecular function,MF),KEGG富集分析到14条通路.通过免疫沉淀结合质谱技术,筛选出在自噬过程中与Hrr25互作的候选蛋白,为研究Hrr25与底物的相互作用及深入了解其调控自噬的分子机制提供新信息.展开更多
Hrr25是酿酒酵母中Ⅰ型酪蛋白激酶家族成员,具有丝氨酸/苏氨酸蛋白激酶活性。其可以通过磷酸化多种蛋白质来发挥作用。Hrr25的底物蛋白质包括自噬相关蛋白、COPⅡ(coat protein complexes Ⅱ)囊泡衣被蛋白Sec24和Sec23、真核翻译起始因...Hrr25是酿酒酵母中Ⅰ型酪蛋白激酶家族成员,具有丝氨酸/苏氨酸蛋白激酶活性。其可以通过磷酸化多种蛋白质来发挥作用。Hrr25的底物蛋白质包括自噬相关蛋白、COPⅡ(coat protein complexes Ⅱ)囊泡衣被蛋白Sec24和Sec23、真核翻译起始因子6、γ-微管蛋白Tub4、延长器复合蛋白1等。另外Hrr25还可以与减数分裂重组蛋白Rec8、核孔蛋白Nup53、转录调节因子Crz1、转录激活因子Haa1等相互作用。Hrr25的多种相互作用蛋白质使其在自噬、囊泡运输、微管组装、减数分裂、有丝分裂、DNA修复、核糖体生物发生和弱有机酸胁迫途径等多种生物过程发挥作用。为了更好地了解Hrr25在各个生物过程中的作用机制以及各个生物过程之间的联系,本文总结了Hrr25的生物学功能及其作用机制,并概述其研究的潜在意义,为Hrr25的进一步研究提供理论依据。展开更多
The paper addresses the problem of target recognition using High-resolution Radar Range Profiles(HRRP).A novel approach of feature extraction and dimension reduction based on extended high order central moments is pro...The paper addresses the problem of target recognition using High-resolution Radar Range Profiles(HRRP).A novel approach of feature extraction and dimension reduction based on extended high order central moments is proposed in order to reduce the dimension of range profiles.Features extracted from radar HRRPs are normalized and smoothed,and then comparative analysis of the similar approaches is done.The range profiles are obtained by step frequency technique using the two-dimensional backscatters distribution data of four different aircraft models.The template matching method by nearest neighbor rules,which is based on the theory of kernel methods for pattern analysis,is used to classify and identify the range profiles from four different aircrafts.Numerical simulation results show that the proposed approach can achieve good performance of stability,shift independence and higher recognition rate.It is helpful for real-time identification and the engineering implements of automatic target recognition using HRRP.The number of required templates could be reduced con-siderably while maintaining an equivalent recognition rate.展开更多
To obtain the radar High Range Resolution (HRR) profile of the slowly moving ground target in strong clutter background, the Phase-Coded Hopped-Frequency (PCHF) waveform is proposed. By multiple-bursts coherent proces...To obtain the radar High Range Resolution (HRR) profile of the slowly moving ground target in strong clutter background, the Phase-Coded Hopped-Frequency (PCHF) waveform is proposed. By multiple-bursts coherent processing, the HRR profile synthesis, target velocity compensation and clutter compression can be accomplished simultaneously. The new waveform is shown to have good ability to suppress ground clutter and good Electronic Counter-CounterMeasures (ECCM) ability as well. The clutter compression performance of the proposed method is verified by the numerical results.展开更多
文摘Hrr25是酿酒酵母中具有丝氨酸/苏氨酸蛋白激酶活性的I型酪蛋白激酶家族成员.在自噬过程中Hrr25可以磷酸化多种蛋白调控自噬过程,然而目前对Hrr25的作用底物分析仍不明确,存在巨大的科研探究价值.Hrr25相互作用蛋白的鉴定可以通过免疫沉淀结合质谱分析来实现.构建了表达Hrr2513Myc蛋白的菌株,以Hrr2513Myc为靶蛋白进行免疫沉淀,将免疫沉淀的样品进行质谱检测.利用Venny2.1软件,得到在诱导自噬条件下与Hrr25特异性相互作用的蛋白.通过DAVID数据库进行基因本体论(gene ontology,GO)和京都基因和基因组百科全书(kyoto encyclopedia of genes and genome,KEGG)基因富集.结果在诱导自噬条件下筛选出321种特异与Hrr25相互作用的蛋白,根据P<0.05,GO富集共得到23项生物过程(biological process,BP)、16项细胞组成(cellular component,CC)、21项分子功能(molecular function,MF),KEGG富集分析到14条通路.通过免疫沉淀结合质谱技术,筛选出在自噬过程中与Hrr25互作的候选蛋白,为研究Hrr25与底物的相互作用及深入了解其调控自噬的分子机制提供新信息.
文摘Hrr25是酿酒酵母中Ⅰ型酪蛋白激酶家族成员,具有丝氨酸/苏氨酸蛋白激酶活性。其可以通过磷酸化多种蛋白质来发挥作用。Hrr25的底物蛋白质包括自噬相关蛋白、COPⅡ(coat protein complexes Ⅱ)囊泡衣被蛋白Sec24和Sec23、真核翻译起始因子6、γ-微管蛋白Tub4、延长器复合蛋白1等。另外Hrr25还可以与减数分裂重组蛋白Rec8、核孔蛋白Nup53、转录调节因子Crz1、转录激活因子Haa1等相互作用。Hrr25的多种相互作用蛋白质使其在自噬、囊泡运输、微管组装、减数分裂、有丝分裂、DNA修复、核糖体生物发生和弱有机酸胁迫途径等多种生物过程发挥作用。为了更好地了解Hrr25在各个生物过程中的作用机制以及各个生物过程之间的联系,本文总结了Hrr25的生物学功能及其作用机制,并概述其研究的潜在意义,为Hrr25的进一步研究提供理论依据。
文摘The paper addresses the problem of target recognition using High-resolution Radar Range Profiles(HRRP).A novel approach of feature extraction and dimension reduction based on extended high order central moments is proposed in order to reduce the dimension of range profiles.Features extracted from radar HRRPs are normalized and smoothed,and then comparative analysis of the similar approaches is done.The range profiles are obtained by step frequency technique using the two-dimensional backscatters distribution data of four different aircraft models.The template matching method by nearest neighbor rules,which is based on the theory of kernel methods for pattern analysis,is used to classify and identify the range profiles from four different aircrafts.Numerical simulation results show that the proposed approach can achieve good performance of stability,shift independence and higher recognition rate.It is helpful for real-time identification and the engineering implements of automatic target recognition using HRRP.The number of required templates could be reduced con-siderably while maintaining an equivalent recognition rate.
基金Supported by the National Natural Science Foundation of China (No.60302009).
文摘To obtain the radar High Range Resolution (HRR) profile of the slowly moving ground target in strong clutter background, the Phase-Coded Hopped-Frequency (PCHF) waveform is proposed. By multiple-bursts coherent processing, the HRR profile synthesis, target velocity compensation and clutter compression can be accomplished simultaneously. The new waveform is shown to have good ability to suppress ground clutter and good Electronic Counter-CounterMeasures (ECCM) ability as well. The clutter compression performance of the proposed method is verified by the numerical results.
