Objective:To compare the genotype frequencies of HLA class-ⅡDRB1 alleles in Giardia(G.)lamblia-infected children.Methods:A total of 490 Egyptian children aged 2-16 years were subjected to microscopic stool examinatio...Objective:To compare the genotype frequencies of HLA class-ⅡDRB1 alleles in Giardia(G.)lamblia-infected children.Methods:A total of 490 Egyptian children aged 2-16 years were subjected to microscopic stool examination to detect G.lamblia infection,and to exclude other intestinal pathogens.On the basis of their microscopic findings,a group of 80 children were chosen as giardiasis cases,another 80 children were confirmed as Giardia free control group by immunochromatographic test,and the remaining children were excluded.Both giardiasis and control groups were then subjected to blood examination to identify their genetic type of HLA-DRB1 alleles.Results:HLA class-ⅡDRB1*03:01 and DRB1*13:01 alleles were significantly associated with G.lamblia infection(P<0.001 for each variable).On the other hand,HLA class-ⅡDRB1*04:02,DRB1*10:01,DRB1*14:01 and DRB1*15:01 alleles were significantly demonstrated in Giardia free children.However,other HLA-DRB1 alleles did not show any significant association with giardiasis.Conclusions:HLA class-ⅡDRB1*03,DRB1*13,DRB1*04,DRB1*10,DRB1*14 and DRB1*15 alleles may be involved in the establishment of host immune response to G.lamblia infection.展开更多
目的:为获得癫痫患者免疫学异常的证据,本实验检测了患者血清中抗脑抗体(Anti-encephalic antibodies,AEAb)及脑组织人类白细胞抗原Ⅱ类抗原(Human leukocyte antigen class Ⅱantigen,HLA class Ⅱ antigen,HLA-Ⅱ类抗原),并与正常对...目的:为获得癫痫患者免疫学异常的证据,本实验检测了患者血清中抗脑抗体(Anti-encephalic antibodies,AEAb)及脑组织人类白细胞抗原Ⅱ类抗原(Human leukocyte antigen class Ⅱantigen,HLA class Ⅱ antigen,HLA-Ⅱ类抗原),并与正常对照组比较。对象及方法:1.用ELISA方法测定37例癫痫患者血清抗脑自身抗体;2.借助免疫组织化学方法观察了HLA—Ⅱ类抗原在脑组织中表达与分布。结果:1.癫痫患者血清抗脑抗体高于正常对照;2.癫痫患者脑组织中星形胶质细胞和小胶质细胞异常表达HLA—Ⅱ类抗原。讨论:本实验证实癫痫患者存在一定程度的自身免疫应答异常。脑组织胶质细胞表面HLA—Ⅱ类抗原表达异常可能通过多种机制参与癫痫发病。展开更多
目的:检测抗原处理相关转运蛋白(transporters associated with antigen processing,TAP)和HLA-Ⅰ类分子在恶性黑素瘤组织中的表达。方法:应用免疫组织化学方法检测77例恶性黑素瘤组织TAP1、TAP2和HLA-Ⅰ类分子的表达,并进行半定量分析,...目的:检测抗原处理相关转运蛋白(transporters associated with antigen processing,TAP)和HLA-Ⅰ类分子在恶性黑素瘤组织中的表达。方法:应用免疫组织化学方法检测77例恶性黑素瘤组织TAP1、TAP2和HLA-Ⅰ类分子的表达,并进行半定量分析,以20例色素痣组织作为对照研究。结果:在77例恶性黑素瘤组织中,TAP1、TAP2和HLA-Ⅰ类分子的阳性表达率分别为23.38%、11.69%和63.64%,HLA-Ⅰ类分子的表达与TAP1和TAP2成正相关;而TAP1、TAP2和HLA-Ⅰ类分子几乎在所有的色素痣组织中都为阳性表达。结论:在恶性黑素瘤组织中TAP1、TAP2和HLA-Ⅰ类分子的表达水平下降,可能与人恶性黑素瘤细胞的免疫逃逸表型有关。TAP1和TAP2的表达下降可能是导致HLA-Ⅰ类分子表达下降的重要原因。展开更多
文摘Objective:To compare the genotype frequencies of HLA class-ⅡDRB1 alleles in Giardia(G.)lamblia-infected children.Methods:A total of 490 Egyptian children aged 2-16 years were subjected to microscopic stool examination to detect G.lamblia infection,and to exclude other intestinal pathogens.On the basis of their microscopic findings,a group of 80 children were chosen as giardiasis cases,another 80 children were confirmed as Giardia free control group by immunochromatographic test,and the remaining children were excluded.Both giardiasis and control groups were then subjected to blood examination to identify their genetic type of HLA-DRB1 alleles.Results:HLA class-ⅡDRB1*03:01 and DRB1*13:01 alleles were significantly associated with G.lamblia infection(P<0.001 for each variable).On the other hand,HLA class-ⅡDRB1*04:02,DRB1*10:01,DRB1*14:01 and DRB1*15:01 alleles were significantly demonstrated in Giardia free children.However,other HLA-DRB1 alleles did not show any significant association with giardiasis.Conclusions:HLA class-ⅡDRB1*03,DRB1*13,DRB1*04,DRB1*10,DRB1*14 and DRB1*15 alleles may be involved in the establishment of host immune response to G.lamblia infection.
文摘目的:为获得癫痫患者免疫学异常的证据,本实验检测了患者血清中抗脑抗体(Anti-encephalic antibodies,AEAb)及脑组织人类白细胞抗原Ⅱ类抗原(Human leukocyte antigen class Ⅱantigen,HLA class Ⅱ antigen,HLA-Ⅱ类抗原),并与正常对照组比较。对象及方法:1.用ELISA方法测定37例癫痫患者血清抗脑自身抗体;2.借助免疫组织化学方法观察了HLA—Ⅱ类抗原在脑组织中表达与分布。结果:1.癫痫患者血清抗脑抗体高于正常对照;2.癫痫患者脑组织中星形胶质细胞和小胶质细胞异常表达HLA—Ⅱ类抗原。讨论:本实验证实癫痫患者存在一定程度的自身免疫应答异常。脑组织胶质细胞表面HLA—Ⅱ类抗原表达异常可能通过多种机制参与癫痫发病。
文摘目的:检测抗原处理相关转运蛋白(transporters associated with antigen processing,TAP)和HLA-Ⅰ类分子在恶性黑素瘤组织中的表达。方法:应用免疫组织化学方法检测77例恶性黑素瘤组织TAP1、TAP2和HLA-Ⅰ类分子的表达,并进行半定量分析,以20例色素痣组织作为对照研究。结果:在77例恶性黑素瘤组织中,TAP1、TAP2和HLA-Ⅰ类分子的阳性表达率分别为23.38%、11.69%和63.64%,HLA-Ⅰ类分子的表达与TAP1和TAP2成正相关;而TAP1、TAP2和HLA-Ⅰ类分子几乎在所有的色素痣组织中都为阳性表达。结论:在恶性黑素瘤组织中TAP1、TAP2和HLA-Ⅰ类分子的表达水平下降,可能与人恶性黑素瘤细胞的免疫逃逸表型有关。TAP1和TAP2的表达下降可能是导致HLA-Ⅰ类分子表达下降的重要原因。
