分别采用商品化的酶联免疫吸附试验(ELISA)抗体检测试剂盒和血凝抑制(HI)试验方法对152份临床血清样本和标准阴阳性血清进行新城疫病毒(NDV)抗体检测,比较间接 ELISA 和 HI 两种方法的相关性。研究结果表明,间接 ELISA 和 HI 两种方法...分别采用商品化的酶联免疫吸附试验(ELISA)抗体检测试剂盒和血凝抑制(HI)试验方法对152份临床血清样本和标准阴阳性血清进行新城疫病毒(NDV)抗体检测,比较间接 ELISA 和 HI 两种方法的相关性。研究结果表明,间接 ELISA 和 HI 两种方法呈显著相关,二者间的相关系数为0.9261。间接 ELISA 和 HI 两种方法检测 NDV 抗体的阳性符合率为96.8%,阴性符合率为92.9%,间接 ELISA 方法是一种敏感、特异、快速的血清学诊断方法,可以用于临床样本的大规模检测。展开更多
Newcastle disease (ND) virus is a leading threat to commercial and domestic poultry in Pakistan. The virus infects and constitutes irreversible impairment to the nervous system, damages the respiratory system, and mar...Newcastle disease (ND) virus is a leading threat to commercial and domestic poultry in Pakistan. The virus infects and constitutes irreversible impairment to the nervous system, damages the respiratory system, and marks severe gastrointestinal lesions leading to heavy mortality in short-living birds and substantial losses in layers and breeders. The continuous emergence and evolution of the virus made it inclined to evade the humoral response and indirectly the circumvention of artificial active immunization. Newcastle disease is caused by the orthoavula genus of the paramyxoviridae family and has shown high genetic diversity even in their genotypes while information regarding enzootic trends of the virus is scanty in Pakistan. A total of 40 tracheal samples of NDV were collected from different commercial broiler farms and 11 isolates of NDV were identified. In the current study, we determined the genetic diversity of the Newcastle disease virus based on the partial sequencing of the fusion protein gene available in the NCBI database. Genetic analysis showed that seven isolates belonged to class I genotype VII and four belonged to class II genotype II. Interestingly, two isolates had epidemiological connections with vaccine-like class II genotype II. Our findings, concerning the recent outbreaks of class I genotype VII and class II genotype II of NDV in vaccinated commercial flocks, suggest possible potential partial mutations in the fusion protein gene. Genetic diversity and formation of the new cleavage site in an important neutralizing protein of wild strain are linked with the potency of artificial active immunization and a major cause of vaccine failure.展开更多
应用商品化酶联免疫吸附试验(BLISA)试剂盒和血凝抑制试验(HI)法,同时测定了173个常规鸡产蛋下降综合症(EDS_(76))抗体送检样品.结果显示,ELISA 法的灵敏度和特异性分别为 HI 法的93.9%和100%.每一样品用这二种方法在相互独立的条件下,...应用商品化酶联免疫吸附试验(BLISA)试剂盒和血凝抑制试验(HI)法,同时测定了173个常规鸡产蛋下降综合症(EDS_(76))抗体送检样品.结果显示,ELISA 法的灵敏度和特异性分别为 HI 法的93.9%和100%.每一样品用这二种方法在相互独立的条件下,检测取得了基本相同的结果,Kappa 值达到了0.97,表明了这二种方法有很好的一致性。二种方法抗体效价的相关性分析表明,相关系数为0.77.在对带有高、中、低抗体和阴性混合血清的重复测定中,发现随着 S/P 值的变小,其标准差也随之减小,显示了较好的重复性.本试验还对阴阳性判别的临界点的设定对试验灵敏度的影响进行了分析。展开更多
文摘Newcastle disease (ND) virus is a leading threat to commercial and domestic poultry in Pakistan. The virus infects and constitutes irreversible impairment to the nervous system, damages the respiratory system, and marks severe gastrointestinal lesions leading to heavy mortality in short-living birds and substantial losses in layers and breeders. The continuous emergence and evolution of the virus made it inclined to evade the humoral response and indirectly the circumvention of artificial active immunization. Newcastle disease is caused by the orthoavula genus of the paramyxoviridae family and has shown high genetic diversity even in their genotypes while information regarding enzootic trends of the virus is scanty in Pakistan. A total of 40 tracheal samples of NDV were collected from different commercial broiler farms and 11 isolates of NDV were identified. In the current study, we determined the genetic diversity of the Newcastle disease virus based on the partial sequencing of the fusion protein gene available in the NCBI database. Genetic analysis showed that seven isolates belonged to class I genotype VII and four belonged to class II genotype II. Interestingly, two isolates had epidemiological connections with vaccine-like class II genotype II. Our findings, concerning the recent outbreaks of class I genotype VII and class II genotype II of NDV in vaccinated commercial flocks, suggest possible potential partial mutations in the fusion protein gene. Genetic diversity and formation of the new cleavage site in an important neutralizing protein of wild strain are linked with the potency of artificial active immunization and a major cause of vaccine failure.
