THE snake venom zinc-metalloproteinases and matrix metalloproteinases (MMP) have the similar active site and biological function. They all belong to the MMP super-family. Some
The haemorrhagin AaH Ⅲ isolated from the snake venom of Agkistrodon acutus is one of the few al-kaline ones in snake venoms. Its crystals belong to space group P212121 with a = 9. 573 4 nm, b = 4. 996 7 nm and c = 4....The haemorrhagin AaH Ⅲ isolated from the snake venom of Agkistrodon acutus is one of the few al-kaline ones in snake venoms. Its crystals belong to space group P212121 with a = 9. 573 4 nm, b = 4. 996 7 nm and c = 4. 728 8 nm. Its crystal structure was determined by the molecular replacement method according to the model of metalloproteinase Adamalysin n from eastern rattlesnake venom. The AaHⅢ structure has been refined by PROLSQ. The final R factor was 0.254 and the RMS deviations of bond lengths and angles were 0. 001 8 nm and 1.5°. The structure comparison suggested that AaHⅢ has a similar structure to other snake venom zinc-metalloproteinases. They all belong to matrix metalloproteinases super-family.展开更多
Haemorrhagins,Which cause local haemorrhage or even death after injection into exper-imental animals,exist widely in many kinds of snake venoms.Almost allthe haemorrhagins reported are zinc-metalloproteinases with hig...Haemorrhagins,Which cause local haemorrhage or even death after injection into exper-imental animals,exist widely in many kinds of snake venoms.Almost allthe haemorrhagins reported are zinc-metalloproteinases with highly conserved amino acid se-quences.Some of them could degrade the proteins in matrices so that they are the im-portant targets for drugs to combat diseases such as arthritis and cancer.Snake venommetalloproteinases can be divided into three classes based on their molecular展开更多
文摘THE snake venom zinc-metalloproteinases and matrix metalloproteinases (MMP) have the similar active site and biological function. They all belong to the MMP super-family. Some
基金Project supported by the Chinese Academy of Sciences, State Key Laboratory of Biomacromolecules and State Education Commission of China.
文摘The haemorrhagin AaH Ⅲ isolated from the snake venom of Agkistrodon acutus is one of the few al-kaline ones in snake venoms. Its crystals belong to space group P212121 with a = 9. 573 4 nm, b = 4. 996 7 nm and c = 4. 728 8 nm. Its crystal structure was determined by the molecular replacement method according to the model of metalloproteinase Adamalysin n from eastern rattlesnake venom. The AaHⅢ structure has been refined by PROLSQ. The final R factor was 0.254 and the RMS deviations of bond lengths and angles were 0. 001 8 nm and 1.5°. The structure comparison suggested that AaHⅢ has a similar structure to other snake venom zinc-metalloproteinases. They all belong to matrix metalloproteinases super-family.
基金Project supported by the State Key Laboratory of Biomacromolecules, Chinese Academy of Science and State Foundation Commission of China.
文摘Haemorrhagins,Which cause local haemorrhage or even death after injection into exper-imental animals,exist widely in many kinds of snake venoms.Almost allthe haemorrhagins reported are zinc-metalloproteinases with highly conserved amino acid se-quences.Some of them could degrade the proteins in matrices so that they are the im-portant targets for drugs to combat diseases such as arthritis and cancer.Snake venommetalloproteinases can be divided into three classes based on their molecular
