Application of extracellular vesicles from mesenchymal stem cells promotes hair growth by regulating human dermal cells and follicles

BACKGROUND Dermal papillae(DP)and outer root sheath(ORS)cells play important roles in hair growth and regeneration by regulating the activity of hair follicle(HF)cells.AIM To investigate the effects of human mesenchymal stem cell-derived extracellular vesicles(hMSC-EVs)on DP and ORS cells as well as HFs.EVs are known to regulate various cellular functions.However,the effects of hMSC-EVs on hair growth,particularly on human-derived HF cells(DP and ORS cells),and the possible mechanisms underlying these effects are unknown.METHODS hMSC-EVs were isolated and characterized using transmission electron microscopy,nanoparticle tracking analysis,western blotting,and flow cytometry.The activation of DP and ORS cells was analyzed using cellular proliferation,migration,western blotting,and real-time polymerase chain reaction.HF growth was evaluated ex vivo using human HFs.RESULTS Wnt3a is present in a class of hMSC-EVs and associated with the EV membrane.hMSC-EVs promote the proliferation of DP and ORS cells.Moreover,they translocateβ-catenin into the nucleus of DP cells by increasing the expression ofβ-catenin target transcription factors(Axin2,EP2 and LEF1)in DP cells.Treatment with hMSC-EVs also promoted the migration of ORS cells and enhanced the expression of keratin(K)differentiation markers(K6,K16,K17,and K75)in ORS cells.Furthermore,treatment with hMSC-EVs increases hair shaft elongation in cultured human HFs.CONCLUSION These findings suggest that hMSC-EVs are potential candidates for further preclinical and clinical studies on hair loss treatment.

Safety and Efficacy of Deeply Rooted^®Nutraceutical for Hair Growth in Women

Hair loss can significantly affect quality of life. Patients seeking treatment for hair loss find that many surgical or medication options involve significant risks or side effects. DEEPLY ROOTED® is a nutritional supplement that combines botanical and natural ingredients with the goal of effectively reducing hair loss and stimulating hair growth—without the side effects of many standard treatments. DEEPLY ROOTED’s active ingredients have both anti-inflammatory and adaptogenic properties, and inhibit dihydrotestosterone without the use of hormones, drugs, or industry by-products. This 3-month prospective study evaluates the effectiveness of DEEPLY ROOTED® for the prevention of hair loss. Healthy female subjects complaining of hair loss were enrolled, and supplied with three months of DEEPLY ROOTED®, without other compensation. The primary endpoint was a significant increase in hair growth. This was measured using anonymously collected patient data to evaluate hair growth and quality, as well as blinded Investigator hair assessment data. Patients who ingested DEEPLY ROOTED® over a three-month period reported noticeable improvement of hair growth (91%), increased hair volume (86%), and improved hair quality (86%). Subjects were noted to exhibit an increase in the number of new hairs, hair thickness and scalp coverage. No significant adverse effects were reported. DEEPLY ROOTED® safely helped over 90% of otherwise healthy women with self-perceived hair thinning to improve hair quality and quantity. The unique combination of botanical and natural ingredients provided a drug-free alternative for the management of hair loss.

Hair Growth Promoting Effect of Trichoxidil<up>TM</up>: A New Natural Compound for Hair Loss

Background: Androgenetic alopecia (AGA) is a most common condition of hair loss. Thus, the present study aimed to investigate the effect of TrichoxidilTM, a phytocomplex obtained from a blend of essential oils, in the treatment of hair loss caused by AGA. Methods: The CCD1072Sk cells were cultured for the 24-hour cell viability assessment and cytotoxicity of TrichoxidilTM. The expression of mRNA levels from KGF, IGF-1, and VEGF in fibroblasts was evaluated by RT-qPCR. Thirty-three volunteers, diagnosed with AGA, men and women, aged between 25 and 50 years, were divided into Control group, without treatment (n = 5);TrichosolTM vehicle group, without active (n = 5);Hydroalcoholic vehicle group, without active (n = 4);TrichosolTM vehicle group, with 2.5% minoxidil (n = 5);Hydroalcoholic vehicle group, with 2.5% minoxidil (n = 5);TrichosolTM group with 2.5% TrichoxidilTM (n = 5) and Hydroalcoholic vehicle group with 2.5% TrichoxidilTM (n = 4) to dermoscopic and histologic. Results: Fibroblasts exhibited higher proliferation when treated with higher concentrations of TrichoxidilTM. TrichoxidilTM significantly increased the expression of KGF, IGF-1, and VEGF mRNA in fibroblasts cells. Analysis of the capillary density showed that TrichoxidilTM associated with TrichosolTM vehicle, was the most effective association. In addition, it was observed an increased more effectively the percentage of anagen phase and reduction of the telogen when compared to other formulations. Conclusion: TrichoxidilTM promoted proliferative effects and positively modulated the expression of growth factors IGF-1, VEGF, and KGF, being a promising candidate for the treatment of hair loss caused by AGA.

Research Progress of Hair Growth Technology

This summarizes the research progress of domestic and foreign experts on the methods and technologies of hair growth,including the hair growth effects of natural plant extracts,mesotherapy and stem cell therapy.Looking forward to the development trend in the field of hair growth,mesotherapy has the advantages of convenient operation,good targeting,fewer side effects,and high cost performance.Combining it with anti-hair loss cosmetic products containing natural plant extracts to treat hair loss has very broad application prospects.

Red organic light-emitting diodes based photobiomodulation therapy enabling prominent hair growth

Hair loss can cause psychological distress.Here,red organic light-emitting diode(OLED)light source is first introduced as the photobiomodulation therapy(PBMT)for hair growth and demonstrated as a promising and non-invasive therapeutic modality for alopecia.OLED exhibits unique advantages of homogeneous irradiation,flexible in form factor,less heat generation.These features enable OLED to be an ideal candidate for wearable PBMT light sources.A systematic study of using red OLEDs to facilitate hair growth was conducted.The results show that OLEDs excellently promote hair regrowth.OLED irradiation can increase the length of the hair by a factor of 1.5 as compared to the control,the hair regrowth area is enlarged by over 3 times after 20 days of treatments.Moreover,the mechanism of OLED that stimulates hair follicle regeneration is investigated invivo by conducting a systematic controlled experiments on mice with or without OLED PBMT.Based on the comprehensive histological and immunofluorescence staining studies,two key factors are identified for red OLEDs to facilitate hair follicle regeneration:(i)increased autophagy during the anagen phase of the hair growth cycle;(ii)increased blood oxygen content promoted by the accelerated microvascular blood flow.

SH-118 Quick Growth Hair Blackening Oil

The SH-118 quick growth hair blackening oil produced by the Shanghai Yifa Health Care Industry Co. is made of effective ingredients extracted from natural wild plants on the principle of Chinese traditional medicine combined with modern scientific technology, There is neither any harmful chemical composition, nor side-effect. The product is a kind of brownish semi-transparent oily liquid, having the functions of aiding general weakness,

CeO_(2)纳米酶对环磷酰胺所致斑秃小鼠毛发生长的影响

目的 探讨氧化铈(CeO_(2))纳米酶对环磷酰胺所致斑秃小鼠模型毛发生长的影响。方法 将雌性C57BL/6J小鼠30只随机分为空白组、模型组和CeO_(2)纳米酶组(300μg/mL),每组10只。模型组和CeO_(2)纳米酶组小鼠采用单次腹腔内注射环磷酰胺注射液,构建斑秃小鼠模型。CeO_(2)纳米酶组小鼠给予300μg/mL CeO_(2)纳米酶外涂于小鼠背部剃毛区,空白组和模型组小鼠均予以等体积的PBS溶液外涂,1次/d,连续27 d。实验结束后(第28天)肉眼观察小鼠背部秃发区毛发生长情况并进行评分;HE染色小鼠背部经处理的皮肤组织计算单位视野内毛囊数量;酶联免疫吸附试验(ELISA)法检测小鼠血清细胞间黏附分子-1(ICAM-1)及内皮细胞白细胞黏附分子-1(ELAM-1)水平;水溶性四唑盐-1法(WST-1)检测皮肤组织丙二醛(MDA)及超氧化物歧化酶(SOD)含量。结果 与空白组相比,模型组小鼠毛发生长及毛发评分显著下降(P<0.05),毛囊数量明显减少(P<0.05),血清ICAM-1及ELAM-1水平升高(P<0.05),皮肤组织MDA含量升高而SOD含量降低(P<0.05);与模型组相比,CeO_(2)纳米酶组毛发生长及毛发评分显著增加(P<0.05),毛囊数量明显增多(P<0.05),血清ICAM-1及ELAM-1水平降低(P<0.05),皮肤组织MDA含量降低而SOD含量升高(P<0.05)。结论 外用CeO_(2)纳米酶对环磷酰胺所致斑秃小鼠模型具有促进毛发再生的作用,为CeO_(2)纳米酶外用治疗斑秃提供了实验依据。

浓缩生长因子治疗雄激素性秃发的回顾性研究

目的:回顾性分析浓缩生长因子(concentrated growth factor,CGF)治疗雄激素性秃发(androgenetic alopecia,AGA)的疗效与安全性。方法:收集使用CGF治疗的60例AGA患者资料。12例为CGF治疗组,48例为联合治疗组。CGF组和联合治疗组均给予CGF治疗,连续注射3次,联合组另给予米诺地尔酊外用,口服非那雄胺(男性)或螺内酯(女性)。通过毛发密度和毛囊单位密度改善程度、毛发镜图像特征变化、治疗前后照片以及医生和患者满意度评价综合评估有效性。同时,记录发生局部及全身不良反应的患者例数,以评估安全性。结果:CGF联合治疗组和CGF组治疗后的平均毛囊单位密度较治疗前分别增加了(18.04±11.15)和(8.81±5.77)个/cm 2(均P<0.05),平均毛发密度较治疗前分别增加了(36.43±21.18)和(16.19±12.62)根/cm 2(均P<0.05)。两组之间在改善毛发密度上差异具有统计学意义(P<0.05),在改善毛囊单位密度上差异无统计学意义(P>0.05)。两组毛囊周围褐色征、黄点征、空毛囊的治疗后改善率均在70%以上,但两组之间差异均无统计学意义(均P>0.05)。两组医生与患者治疗后满意率均在90%以上。60例患者中仅有2例局部注射部位疼痛明显,3例患者头皮瘙痒。结论:CGF治疗AGA安全有效,联合使用米诺地尔酊和非那雄胺/螺内酯,效果更佳。

自噬在毛发再生中的作用

背景:在生物医学界,自噬是一个快速发展的研究热点,许多研究人员正在积极探究多种疾病中自噬的分子调控机制,但是自噬在毛发生长中所起的作用仍存在许多未知。目的:综述目前自噬在毛发生长与再生中的研究进展及应用价值,了解自噬在毛发生长中的作用,以探究自噬在病理性脱发中的发病机制,为研究治疗脱发的药物提供新思路。方法:应用计算机在中国知网(CNKI)、PubMed数据库中,以“毛囊生长,毛发生长,毛发再生,自噬相关蛋白,自噬活性,自噬相关基因,自噬”或“hair growth,hair follicle,hair regeneration,autophagy”为检索词进行检索。查阅国内外近年来关于自噬、毛发生长和自噬在毛发生长中作用的研究进展进行总结,排除与文章主题内容不相符的文献,最终纳入78篇文献进行结果分析。结果与结论:(1)自噬是真核生物的正常代谢过程,具有复杂的分子机制和功能特性,既有利于细胞生存,又可促进细胞死亡。(2)脱发相关疾病与自噬活性的改变有关,且自噬活性可调控毛发生长周期;敲除或过表达自噬相关基因可改变毛发生长状态,已发现有多条自噬相关信号通路与毛囊生长有关;自噬的激活剂或抑制剂可用于治疗或预防脱发疾病。

超高效液相色谱-串联质谱法测定洗发水中14种防脱育发类药物的含量

提出了超高效液相色谱-串联质谱法测定洗发水中西咪替丁、米诺地尔、美拉酮宁、香草酸甲酯、酮康唑、比马前列素、非那雄胺、塞替匹仑、尼鲁米特、氟罗地尔、螺内酯、氟他胺、拉坦前列素、度他雄胺等14种防脱育发类药物含量的方法。样品经水溶解、乙腈提取后,用氨基固相萃取柱净化。以Waters Accquity UPLC BEH C18色谱柱为固定相,以乙腈-含0.1%(体积分数)甲酸的2 mmol·L^(-1)乙酸铵溶液为流动相体系进行梯度洗脱;分离后的14种目标物经电喷雾离子源正、负离子模式扫描,多反应监测模式检测。结果表明:14种目标物的质量浓度在1.0~50.0μg·L^(-1)内与对应的峰面积呈线性关系,检出限(3S/N)为0.05~3.0μg·kg^(-1);对空白样品进行3个浓度水平的加标回收试验,回收率为83.6%~99.0%,测定值的相对标准偏差(n=6)为5.6%~15%;方法用于10个洗发水样品分析,一个样品中检出米诺地尔、西咪替丁、塞替匹仑、酮康唑、尼鲁米特、氟罗地尔,另一个样品中检出美拉酮宁,检出量为0.4~47.8μg·kg^(-1)。

水禽羽毛生长机制及其营养调控研究进展

羽毛是水禽的关键经济性状,也是重要的外貌特征,羽毛在飞翔、保温、游泳、防护、调控温度等方面都具有重要作用。羽毛的结构复杂,羽毛类型及组成也大不相同,另外羽毛的发育需经过三代逐级生长才最终长成。毛囊的生长发育在羽毛生长发育中扮演着重要角色,从毛囊的结构及组成,毛囊发生发育的过程到毛囊生长期、退行期和静止期3个时期的更新,初级毛囊和次级毛囊的分类等无一不显示出毛囊发育是羽毛生长发育的关键。羽毛的生长发育是一个极其复杂的过程,除了Wnt/β-连环蛋白(β-catenin)信号通路、成纤维细胞生长因子(fibroblast growth factor,FGF)家族、SHH信号通路、Notch信号、Hox基因、表皮生长因子(EGF)和血小板衍生生长因子(PDGF)等多种因素参与外,还受到各种营养物质的调控,如蛋白质、氨基酸、维生素、矿物元素等。然而,近年来随着养禽业集约化发展和旱养规模化的进步,水禽羽毛问题(如生长缓慢、无光泽、异常脱落等)日益突出,严重影响其羽毛品质及屠宰外观,导致极大经济损失。作者主要从水禽羽毛生长、毛囊的结构与发育等方面阐述了参与水禽羽毛生长和毛囊发育的相关因子,并整理归纳了对羽毛生长发育具有积极调控作用的营养物质,以期为解决水禽羽毛问题和推进水禽产业发展提供一定的参考。

防脱育发草本精粹对毛发生长及其抗氧化、抗炎的性质研究

目的 探究防脱育发草本精粹防脱育发能力。方法 通过细胞计数试剂盒8测定防脱育发草本精粹对真皮乳头细胞(DPC)增殖与抗氧化能力作用,利用实时荧光定量PCR检测防脱育发草本精粹对DPC抗氧化、抗炎症关键基因的表达影响,通过动物实验验证其对于毛发生长的作用。结果 防脱育发草本精粹(250μg/ml)能显著促进DPC增殖与抗氧化能力,显著抑制白细胞介素-6(IL-6)和促进抗氧化相关基因:谷氨酸-半胱氨酸连接酶修饰剂亚基基因(GCLM)、血红素加氧酶1基因(HMOX1)、铁蛋白重链1基因(FTH1)、铁蛋白轻链基因(FTL)、NAD(P)H:醌氧化还原酶1(NQO1)的表达,差异有统计学意义(P <0.05)。动物实验验证显示,防脱育发草本精粹缩短了小鼠毛囊的休止期。结论 该防脱育发草本精粹显著促进DPC的增殖、抗氧化及抗炎症能力,缩短毛囊的休止期和促进毛发生长。

Polyphenol-Rich Olive Mill Wastewater Extract and Its Potential Use in Hair Care Products

In this work, the influence of phenol-enriched olive mill wastewater (OMWW) extract on hair growth was investigated in vitro on human follicle dermal papilla cells. OMWW has already shown great potential for use in skincare products, and its high polyphenol content is predestined to have a positive effect on hair growth. The studies included caffeine, a positive modulator of hair growth, and dihydrotestosterone, which causes hair loss in vivo, as controls. The impact of the investigated compounds on hair growth was evaluated by studies on cell viability and proliferation, the release of growth factors (insulin-like growth factor-1 and vascular endothelial growth factor), and the reduction of reactive oxygen species formation. OMWW showed a positive influence on the proliferation of the human follicle dermal papilla cells. Moreover, the extract leads to a significantly increased secretion of insulin-like growth factor-1, and a considerable reduction in reactive oxygen species formation was observed. Overall, our results show that the investigated phenol-enriched OMWW extract is a promising ingredient for hair care to improve hair growth, prevent hair loss due to oxidative stress and maintain a healthy scalp.

Effect of CO_2 Elevation on Root Growth and Its Relationship with Indole Acetic Acid and Ethylene in Tomato Seedlings

A hydroponic experiment was carried out to study the effect of elevated carbon dioxide(CO2) on root growth of tomato seedlings.Compared with the control(350 μL L-1),CO2 enrichment(800 μL L-1) significantly increased the dry matter of both shoot and root,the ratio of root to shoot,total root length,root surface area,root diameter,root volume,and root tip numbers,which are important for forming a strong root system.The elevated CO2 treatment also significantly improved root hair development and elongation,thus enhancing nutrient uptake.Increased indole acetic acid concentration in plant tissues and ethylene release in the elevated CO2 treatment might have resulted in root growth enhancement and root hair development and elongation.

Vitamin B12 Activates the Wnt-Pathway in Human Hair Follicle Cells by Induction of <i>beta</i>-Catenin and Inhibition of Glycogensynthase Kinase-3 Transcription

Background and Objectives: Micrograft transplantation is accompanied by a transient induction of telogen in transplanted hair follicles (HF), which might be avoided by supporting the metabolic pathways of the micrograft during the ex vivo period. Vitamin B12 (cobalamin) has been suggested to influence HF growth and cycling in humans, but the mechanisms are unclear. Method: HFs were obtained from patients undergoing routine micrograft transplantation and were cultured for 5 days in Dulbecco’s modified Eagles Medium, supplemented with different amounts of vitamin B12. Hair shaft elongation (HSE) of the isolated HFs as well as quantitative changes of mRNA for beta-catenin, glykogensynthase kinase-3 (GSK-3) and TCF/Lef-1 in HF cells were determined. Results: In vitro HSE demonstrated a dose dependent induction of HSE after stimulation with 2.5 ug/ml and 25 ug/ml vitamin B12 (6.2 +/- 2.1% and 15.4 +/- 3.8% respectively). A dose dependent induction of beta-catenin-mRNA could be demonstrated in cultured HFs after stimulation with 2.5 ug/ml and 25 ug/ml vitamin B12 (fold change compared to DMEM: 9.5 +/- 2.7, p < 0.05 and 23.1 +/- 7.4, p < 0.01;respectively). Concomitantly the amounts of GSK-3 were significantly reduced after stimulation with 25 ug/ml vitamin B12 (fold change compared to DMEM: 0.76 +/- 0.12, p < 0.05). Conclusions: Our data demonstrate a hair growth promoting effect of vitamin B12 in vitro. This effect is accompanied by the modulation of intracellular signal transduction molecules of the wnt-pathway and might promote hair growth after micrograft transplantation.

乳酸促脂溢性脱发小鼠毛发生长研究

通过除去C57BL/6小鼠背部毛发及涂抹睾酮建立脂溢性脱发模型,研究乳酸对脂溢性脱发小鼠毛发生长,毛囊生长周期及生长因子的影响。将60只C57BL/6小鼠随机分为正常组,模型组,米诺地尔酊阳性组,乳酸高、中、低剂量组,并分别在脱毛区涂抹相对应的药物。连续给药24天,记录小鼠皮肤颜色变化时间、脱毛区毛发生长状况以及毛囊组织学特征和毛囊数目的变化;使用Elisa试剂盒检测小鼠体内KGF,VEGF和TGF-β1的质量浓度。结果表明,与模型组、正常组相比较,乳酸能显著促进脂溢性脱发小鼠毛发生长,增加小鼠毛发重量和给药实验区域皮肤毛囊数量,同时上调小鼠皮肤中KGF和VEGF质量浓度,降低小鼠皮肤中TGF-β1质量浓度,差异有统计学意义(P<0.05),其中以中剂量10g/L乳酸溶液效果最佳,可加快小鼠毛发生长、促进小鼠毛囊生长期转化进程,抑制脂溢性脱发症状,其疗效与阳性药米诺地尔酊效果相当(P>0.05),可制成日用洗护产品预防脱发,为脂溢性脱发患者提供更多选择。

毛发移植术中两种受损单株生长期毛囊单位移植效果观察

目的毛囊损伤是毛发移植术中常见的现象,本研究拟对两种常见术中损伤的单株生长期毛囊进行移植并观察1年后的生长效果,为减少毛囊资源浪费,提高利用率,增加移植密度,提高手术效果提供参考。方法选取本院整形科2017年10月至2020年1月的52例前额发际线缺陷的手术患者作为研究对象,其中男24例,女28例。移植单位数:1,450~2,827株。选取正常、毛乳头缺失和外毛根鞘下1/2缺损失的生长期单株毛囊单位作为研究对象,分别种植在3个固定区域,观察1年后的生长效果。结果正常单株毛囊单位存活率为(88.46±5.26)%;毛乳头缺失的毛囊单位存活率为(65.13±9.07)%,生长方向较自然,毛发光泽较好;外毛根鞘缺失下1/2的毛囊存活率(17.37±4.568)%,有细发或生长方向不自然的情况,与皮肤呈角度偏大,有部分贴紧头皮,三者之间差异存在统计学意义(P<0.05)。结论外毛根鞘缺失下1/2的毛囊单位的存活率低于毛乳头缺失的毛囊单位,且两者的存活率均低于正常毛囊单位。两种受损的毛囊可以种植在发际线以上的隐蔽位置或与正常的毛囊同时种植。同时,医护人员在手术中应提高分离精度,减少因提取和切割不当造成毛囊单位的损伤,减少损失,提高利用率。

降香提取物对脂多糖诱导的RAW264.7细胞的抗炎、抗氧化作用和对人毛乳头细胞的促毛发生长作用

目的:探讨降香提取物(DOE)对脂多糖(LPS)诱导的RAW264.7细胞的抗炎及抗氧化作用及其对人毛乳头细胞(h DPCs)的促毛发生长作用。方法:将RAW264.7细胞分为空白对照组、模型组、4%DOE组和8%DOE组。空白对照组不予处理,模型组使用LPS诱导体外炎症模型,4%DOE组和8%DOE组用LPS处理后分别加入4%和8%的DOE,分别采用Griess法和酶联免疫吸附试验(ELISA)法测定各组细胞培养上清液中一氧化氮(NO)、肿瘤坏死因子α(TNF-α)的含量,DCFH-DA荧光探针检测各组细胞内的活性氧(ROS)含量。体外培养人毛囊分离的hDPCs,设置空白对照组、4%DOE组和8%DOE组,采用CCK-8法检测细胞活力,RT-qPCR法检测毛发生长因子CTNNB1和毛发抑制因子DKK1基因的表达,western blotting法检测β-连环蛋白(β-catenin)蛋白表达水平。结果:与模型组相比,4%DOE组和8%DOE组RAW264.7细胞NO、TNF-α、ROS含量降低(均P<0.05)。与空白对照组相比,4%DOE组和8%DOE组hDPCs细胞活力升高,CTNNB1基因和β-catenin蛋白表达上调,且DKK1基因表达下调(均P<0.05)。结论:DOE对LPS诱导的RAW264.7巨噬细胞具有抗炎和抗氧化作用,并且可以通过增加hDPCs的活力及调节关键基因的表达促进毛发生长。

生长激素治疗毛发-鼻-指(趾)综合征Ⅰ型一例报告

随访深圳市儿童医院内分泌科经治的1例毛发-鼻-指(趾)综合征(trichorhinophalangeal syndrome, TRPS)Ⅰ型患儿应用生长激素治疗的效果,并进行文献复习。本文报道并总结生长激素治疗TRPSⅠ型患儿的疗效,除改善矮身材外,首次报道其促进毛发生长。通过总结分析生长激素治疗TRPSⅠ型患儿的效果,以提高临床医生对TRPS的认识。