Objective:To identify the larvieidal activity of the seagrass extracts.Methods:Seagrass extracts,Syringodium isoelifolium(.S.isoetifolium),Cymodocea serrulata and Hulophila beccarii,were dissolved in DMSO to prepare a...Objective:To identify the larvieidal activity of the seagrass extracts.Methods:Seagrass extracts,Syringodium isoelifolium(.S.isoetifolium),Cymodocea serrulata and Hulophila beccarii,were dissolved in DMSO to prepare a graded series of concentration.Batches of 25 early 4th instars larvae of Aedes aegypti(Ae.aegypti) were transferred to 250 ml.enamel bowl containing199 mL of distilled water and 1 mL of plant extracts(0.01 mg- 0.1 mg).After 24 h the mortality rate was identified with the formulae[(%of lest mortality-%of control mortality)/(100-%of control morlality)]× 100.Each experiment was conducted with three replicates and a concurrent control group.A control group consisted of 1 mL of DMSO and 199 mL of distilled water only.Results:The root extract of S.isoelifolium showed maximum larvicidal activity with minimum concentration of extract of LC_(50)= 0.0604 ± 0.0040)μg/mL with lower confidence limit(LCL)-upper confidence limit(UCL) =(0.051-0.071) and LC_(90)=0.0972 μg/mL followed by leaf extract of S.isoelifolium showed LC_(50)=(0.062 ± 0.005)μ'g/mL.The regression equation of pool and leaf extract of S.isoelifolium for4th instar larvae were Y =4.909- 1.32x(R^2= 0.909) and Y= 2.066+ 1.2x(R^2 =0.897) respectively.The results of the preliminary photochemical constituents shows the presence of saponin,steroids,terpenoid,phenols,protein and sugars.Conclusions:from the present study the ethanolic extracts of seagrass of S.isoelifolium possesses lead compound for development of larvieidal activity.展开更多
基金Supported by Ministry of Environment and Forest[Grant No.D.O.NO.3/2/2004 CS(M)-11.12.2006]
文摘Objective:To identify the larvieidal activity of the seagrass extracts.Methods:Seagrass extracts,Syringodium isoelifolium(.S.isoetifolium),Cymodocea serrulata and Hulophila beccarii,were dissolved in DMSO to prepare a graded series of concentration.Batches of 25 early 4th instars larvae of Aedes aegypti(Ae.aegypti) were transferred to 250 ml.enamel bowl containing199 mL of distilled water and 1 mL of plant extracts(0.01 mg- 0.1 mg).After 24 h the mortality rate was identified with the formulae[(%of lest mortality-%of control mortality)/(100-%of control morlality)]× 100.Each experiment was conducted with three replicates and a concurrent control group.A control group consisted of 1 mL of DMSO and 199 mL of distilled water only.Results:The root extract of S.isoelifolium showed maximum larvicidal activity with minimum concentration of extract of LC_(50)= 0.0604 ± 0.0040)μg/mL with lower confidence limit(LCL)-upper confidence limit(UCL) =(0.051-0.071) and LC_(90)=0.0972 μg/mL followed by leaf extract of S.isoelifolium showed LC_(50)=(0.062 ± 0.005)μ'g/mL.The regression equation of pool and leaf extract of S.isoelifolium for4th instar larvae were Y =4.909- 1.32x(R^2= 0.909) and Y= 2.066+ 1.2x(R^2 =0.897) respectively.The results of the preliminary photochemical constituents shows the presence of saponin,steroids,terpenoid,phenols,protein and sugars.Conclusions:from the present study the ethanolic extracts of seagrass of S.isoelifolium possesses lead compound for development of larvieidal activity.
