Long noncoding RNAs HAND2-AS1 ultrasound microbubbles suppress hepatocellular carcinoma progression by regulating the miR-873-5p/tissue inhibitor of matrix metalloproteinase-2 axis

BACKGROUND Increasing data indicated that long noncoding RNAs(lncRNAs)were directly or indirectly involved in the occurrence and development of tumors,including hepatocellular carcinoma(HCC).Recent studies had found that the expression of lncRNA HAND2-AS1 was downregulated in HCC tissues,but its role in HCC progression is unclear.Ultrasound targeted microbubble destruction mediated gene transfection is a new method to overexpress genes.AIM To study the role of ultrasound microbubbles(UTMBs)mediated HAND2-AS1 in the progression of HCC,in order to provide a new reference for the treatment of HCC.METHODS In vitro,we transfected HAND2-AS1 siRNA into HepG2 cells by UTMBs,and detected cell proliferation,apoptosis,invasion and epithelial-mesenchymal transition(EMT)by cell counting kit-8 assay,flow cytometry,Transwell invasion assay and Western blotting,respectively.In addition,we transfected miR-837-5p mimic into UTMBs treated cells and observed the changes of cell behavior.Next,the UTMBs treated HepG2 cells were transfected together with miR-837-5p mimic and tissue inhibitor of matrix metalloproteinase-2(TIMP2)overexpression vector,and we detected cell proliferation,apoptosis,invasion and EMT.In vivo,we established a mouse model of subcutaneous transplantation of HepG2 cells and observed the effect of HAND2-AS1 silencing on tumor formation ability.RESULTS We found that UTMBs carrying HAND2-AS1 restricted cell proliferation,invasion,and EMT,encouraged apoptosis,and HAND2-AS1 silencing eliminated the effect of UTMBs.Additionally,miR-873-5p targets the gene HAND2-AS1,which also targets the 3’UTR of TIMP2.And miR-873-5p mimic counteracted the impact of HAND2-AS1.Further,miR-873-5p mimic solely or in combination with pcDNA-TIMP2 had been transformed into HepG2 cells exposed to UTMBs.We discovered that TIMP2 reversed the effect of miR-873-5p mimic caused by the blocked signalling cascade for matrix metalloproteinase(MMP)2/MMP9.In vivo results showed that HAND2-AS1 silencing significantly inhibited tumor formation in mice.CONCLUSION LncRNA HAND2-AS1 promotes TIMP2 expression by targeting miR-873-5p to inhibit HepG2 cell growth and delay HCC progression.

Controversy of hand-assisted laparoscopic colorectal surgery

Laparoscopically assisted colorectal procedures are time-consuming and technically demanding and hence have a long steep learning curve. In the technical demand, surgeons need to handle a long mobile organ, the colon, and have to operate on multiple abdominal quadrants, most of the time with the need to secure multiple mesenteric vessels. Therefore, a new surgical innovation called hand-assisted laparoscopic surgery (HALS) was introduced in the mid 1990s as a useful alternative to totally laparoscopic procedures. This hybrid operation allows the surgeon to introduce the non-dominant hand into the abdominal cavity through a special hand port while maintaining the pneumoperitoneum. A hand in the abdomen can restore the tactile sensation which is usually lacking in laparoscopic procedures. It also improves the eye-to-hand coordination, allows the hand to be used for blunt dissection or retraction and also permits rapid control of unexpected bleeding. All of those factors can contribute tremendously to reducing the operative time. Moreover, this procedure is also considered as a hybrid procedure that combines the advantages of both minimally invasive and conventional open surgery. Nevertheless, the exact role of HALS in colorectal surgery has not been well defined during the advanced totally laparoscopic procedures. This article reviews the current status of hand-assisted laparoscopic colorectal surgery as a minimally invasive procedure in the era of laparoscopic surgery.

Learning curve for hand-assisted laparoscopic D2 radical gastrectomy

AIM:To describe the learning curves of hand-assisted laparoscopic D2 radical gastrectomy(HALG) for the treatment of gastric cancer.METHODS:The HALG surgical procedure consists of three stages:surgery under direct vision via the port for hand assistance,hand-assisted laparoscopicsurgery,and gastrointestinal tract reconstruction.According to the order of the date of surgery,patients were divided into 6 groups(A-F) with 20 cases in each group.All surgeries were performed by the same group of surgeons.We performed a comprehensive and indepth retrospective comparative analysis of the clinical data of all patients,with the clinical data including general patient information and intraoperative and postoperative observation indicators.RESULTS:There were no differences in the basic information among the patient groups(P > 0.05).The operative time of the hand-assisted surgery stage in group A was 8-10 min longer than the other groups,with the difference being statistically significant(P = 0.01).There were no differences in total operative time between the groups(P = 0.30).Postoperative intestinal function recovery time in group A was longer than that of other groups(P = 0.02).Lengths of hospital stay and surgical quality indicators(such as intraoperative blood loss,numbers of detected lymph nodes,intraoperative side injury,postoperative complications,reoperation rate,and readmission rate 30 d after surgery) were not significantly different among the groups.CONCLUSION:HALG is a surgical procedure that can be easily mastered,with a learning curve closely related to the operative time of the hand-assisted laparoscopic surgery stage.

Hand-assisted laparoscopic splenectomy is a useful surgical treatment method for patients with excessive splenomegaly: A metaanalysis

BACKGROUND Hand-assisted laparoscopic splenectomy(HALS) can help overcome the drawbacks of laparoscopic splenectomy(LS) while maintaining its advantages.AIM To evaluate the efficacy and advantages of HALS for splenomegaly.METHODS The relevant literature was reviewed using the PubMed, EMBASE, Cochrane,Ovid Medline, and Wanfang databases to compare the clinical outcomes of HALS and LS. Odds ratios or mean differences were calculated with 95% confidence intervals for fixed-effects and random-effects models. Overall, 754 patients from16 trials who met the inclusion criteria were selected.RESULTS In pure splenectomy, blood loss volume(P < 0.001) and conversion rate(P =0.008) were significantly lower in the HALS group than in the LS group.Conversely, for splenomegaly, the operative time(P = 0.04) was shorter and blood loss volume(P < 0.001) and conversion rate(P = 0.001) were significantly lower in the HALS group than in the LS group. However, no significant difference was observed in hospital stay length, blood transfusion, time to food intake, complications, or mortality rate between the two groups. Moreover, in splenectomy and devascularization of the upper stomach(DUS), the operative time(P = 0.04) was significantly shorter and blood loss volume(P < 0.001) andconversion rate(P = 0.05) were significantly lower in the HALS + DUS group than in the LS + DUS group. However, no significant difference was observed in hospital stay length, timing of diet, and complications between the two groups.CONCLUSION HALS is an ideal surgical treatment method for splenomegaly because it can maximize the benefits for patients while maintaining the advantages of LS.

Surgical outcomes of hand-assisted laparoscopic liver resection vs. open liver resection: A retrospective propensity scorematched cohort study

Objective: Hand-assisted laparoscopic liver resection has the advantages of open and laparoscopic surgeries.There is still lack of comparison of surgical outcomes between hand-assistied laparoscopic liver resection(HALLR)and open liver resection(OLR). This study compared the surgical outcomes of the two approaches between wellmatched patient cohorts.Methods: Patients who received liver resection during January 2014 and October 2017 in Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College were included in this retrospective study. Propensity score matching(PSM) was performed to reduce selection bias between the two groups. Operation and short-term surgical outcomes were compared between the well matched groups.Results: During this period, 232 patients with a median age of 55.1 years old received OLR, while 49 patients with a median age of 54.7 years old received HALLR. Compared with HALLR group, OLR group has a higher proportion in male patients(190/232, 81.9% vs. 34/49, 69.4%, P=0.048) and lower albumin(43.2±4.5 vs. 44.8±3.7,P=0.020). After PSM, 49 patients from each group were included in the following analysis. Two groups were well balanced in their baseline characteristics, liver functions, preoperative treatments, abdominal surgery history, and surgical difficulty. None perioperative mortality was observed in both groups. Operation time and postoperative complications were similar in two groups(P=0.935, P=0.056). The HALLR group showed less bleeding amount(177.8±217.1 mL vs. 283.1±225.0 mL, P=0.003) and shorter postoperative stay period(6.9±2.2 d vs. 9.0±3.5 d,P=0.001).Conclusions: We demonstrated that hand-assisted laparoscopic surgery is feasible and safe for liver resection,including some difficult cases. HALLR can provide better bleeding control and faster recovery after surgery.

Hand-assisted laparoscopic restorative proctocolectomy for ulcerative colitis

AIM To evaluate the utility of hand-assisted laparoscopic restorative proctocolectomy(HALS-RP) compared with the conventional open procedure(OPEN-RP).METHODS Fifty-one patients who underwent restorative total proctocolectomy with rectal mucosectomy and ileal pouch anal anastomosis between January 2008 and July 2015 were retrospectively analyzed.Twentythree patients in the HALS-RP group and twentyfour patients in the OPEN-RP group were compared.Four patients who had purely laparoscopic surgery were excluded.Restorative total proctocolectomy was performed with mucosectomy and a hand-sewn ilealpouch-anal anastomosis.Preoperative comorbidities,intraoperative factors such as blood loss and operative time,postoperative complications,and postoperative course were compared between two groups.RESULTS Patients in both groups were matched with regards to patient age,gender,and American Society of Anesthesiologists score.There were no significant differences in extent of colitis,indications for surgery,preoperative comorbidities,and preoperative medications in the two groups.The median operative time for the HALS-RP group was 369(320-420) min,slightly longer than the OPEN-RP group at 355(318-421) min; this was not statistically significant.Blood loss was significantly less in HALS-RP [300(230-402) m L] compared to OPEN-RP [512(401-1162) m L,P = 0.003].Anastomotic leakage was noted in 3 patients in the HALS-RP group and 2 patients in the OPEN-RP group(13% vs 8.3%,NS).The rates of other postoperative complications and the length of hospital stay were not different between the two groups.CONCLUSION HALS-RP can be performed with less blood loss and smaller skin incisions.This procedure is a feasible technique for total proctocolectomy for ulcerative colitis.

Abnormal Secondary Growth and Histochemical Localization of Alkaloids in Root System of Aconitum flavum Hand.-Mazz.

[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.

On the Influence Factors of Second-hand House Prices in First-tier Cities——Empirical Analysis Based on Panel Data

With the arrival of the ＂housing stock＂ in first - tier cities, the second - handhousing^market will become the dominant property market. This ardcle aim to the first - tiercities of second - hand housing prices and new home price index for the empirical analysis, thedata related to the cointegration analysis found that the result of the first -tier cities real estatemarket in China, the new home price index is the significant factors influencing the second -hand house price indexi For Beijing, Shanghai second - hand housing and new home price in-dex time series johans test, found that there exists cointegration relationship between two varia-bles,the new city real estate market prices out of a line on the secondary market have clearguide. Therefore, the real estate market regulation aiming at the first -tier cities and the＂housing stock＂ should take the second - hand housing market as the main direction, startingwith the sale price and influencing factors of new houses. At the same time, in different cities,we should adhere to the city＇ s policies, reflect the policy differentiation, promote the reformof the real estate supply side, and promote the return of housing properties.

光子晶体结构生色真丝织物的制备及应用研究

为实现真丝织物的结构生色,文章分别利用真空辅助过滤法和手绘法在白色真丝织物上构筑光子晶体结构。进一步探究真空辅助过滤法中微球组装液体积浓度对结构色的影响规律,分析微球在真丝织物上形成光子晶体运动机制,讨论真丝织物上光子晶体结构色的虹彩效应,并探究手绘法在真丝织物光子晶体结构生色中的应用效果。结果表明:真空辅助过滤法中聚(苯乙烯-甲基丙烯酸),即P(St-MAA)微球组装液的体积浓度为2.4 L/m^(2)时,真丝织物的两面能同时获得光子晶体结构色,此时正反面结构色的反射率峰值在18%左右;在真空辅助过滤法中,P(St-MAA)微球在真丝织物上形成光子晶体结构的过程遵循“先反后正”堆积原则,即织物反面先形成光子晶体,随后微球进一步透过织物堆积在正面。此外,该光子晶体结构生色真丝织物的虹彩效应不明显,与常规染料或颜料着色效果接近;手绘法可以获得图案化的光子晶体生色结构,较适合于个性化加工。该研究为光子晶体结构生色在真丝织物着色中的应用提供参考。

免打结双倒刺线手工缝合法在机器人辅助全胃切除术中的应用及疗效分析(附手术视频)

目的:探讨免打结双倒刺线手工缝合法在机器人辅助根治性全胃切除术后的近期临床疗效。方法:回顾性分析2020年10月—2023年7月吉林大学第一医院普通外科中心胃结直肠外科行机器人辅助根治性全胃切除术的42例胃体或贲门癌患者的临床病理资料,主要包括手术时间、吻合时间、术中出血量、术后住院时间、术后并发症及随访情况。结果:手术时间(290±37)min,吻合时间(23±3)min,术中出血量20(20,100)m L,术后住院时间(8±1)d,术后并发症发生率为33.3%(14/42)。42例患者失访2例,其余40例患者随访时间1~34个月,中位随访时间为19个月。结论:患者行机器人辅助根治性全胃切除术后,采用免打结双倒刺线手工缝合食管空肠法重建消化道,不会增加并发症,且术后近期吻合口瘘和吻合口狭窄发生率较低。

手助腹腔镜下活体供肾切取手术时间的影响因素及其与术后并发症的关系分析

目的探究手助腹腔镜下活体供肾切取手术时间的影响因素,并探究其与术后并发症严重程度间的关系。方法回顾性分析91例行手助腹腔镜下活体供肾切取术的供者的临床资料,分析供者术前基线资料与手术时间之间的相关性,研究手术时间与术后并发症的关系并确定手术时间的阈值。结果肾动脉数量多、肾周脂肪及肾脏后侧脂肪较厚、罹患代谢综合征、梅奥粘连概率(MAP)评分及Clavien-Dindo评分较高均会导致手术时间延长。通过分析受试者工作特征(ROC)曲线发现,当手术时间≥138 min时,供者术后并发症的发生率显著升高(P<0.05)。结论对于具有多支肾动脉、肾周及肾脏后侧脂肪厚度较厚、罹患代谢综合征、MAP评分及Clavien-Dindo评分较高的供者,可选择手术经验更为丰富的医师,做好充分的术前准备并在术后予以密切关注,以及时发现术后并发症并降低并发症的严重程度,改善供者预后。

太极云手联合机器人辅助训练对脑卒中患者手功能的影响

目的:探讨太极云手结合机器人辅助训练对脑卒中患者手功能的康复效果。方法:将60例脑卒中患者随机分为治疗组(n=30)和对照组(n=30),两组均接受常规康复治疗,治疗组接受太极云手训练结合结合机器人辅助训练,对照组接受机器人辅助训练。两组的训练频率为60min/天,5天/周,持续治疗8周。于治疗前、治疗4周后、治疗8周后采用Fugl-Meyer运动功能量表(Fugl-Meyer motor assessment,FMA)腕手部分、Wolf运动功能量表(Wolf motor function test,WMFT)、箱块测试(Box and Block test,BBT)、握力和捏力来评估受试者的手功能。结果:两组患者治疗后与治疗前比较,差异具有统计学意义(P<0.001)。治疗4周后组间比较差异不具有统计学意义(P>0.05);治疗8周后组间比较,WMFT差异具有统计学意义(P<0.05),FMA腕手部分、BBT、握力和捏力不具有统计学差异(P>0.05)。结论:太极云手结合机器人辅助训练能够改善脑卒中患者的手功能。

一种基于STM32单片机的手部运动机能康复训练系统设计

针对脑卒中等疾病引起的手部运动机能减退以及由于长期活动不畅可能导致肌肉萎缩甚至手部功能逐步丧失问题,提出一种基于STM32单片机的手部康复训练系统,该系统由运动辅助训练模块和康复训练动作示范模块组成。STM32处理器通过设定、保存并记录一个训练周期的康复示范动作全过程,并由带高精度编码器的直流伺服电机配合减速器完成各关节设定动作的执行。利用所提出的从动式康复训练方法,经过阶段性训练,可以在一定程度上保持或者增强手部各关节的运动机能,达到防止肌肉萎缩或关节功能退化等问题的产生,有效提升手部功能康复的效果。

MALDI-TOF-MS法对重组人内皮抑素蛋白分子质量测定

肿瘤的生长依赖于血管的生成,新生血管不仅为肿瘤生长提供必需的营养物质,而且为肿瘤细胞扩散提供了重要的途径[1].1997年哈佛大学的O'Reilly等[2]发现了一种内源性新血管生成抑制因子内皮抑素(Endostatin),显示出特异抑制激活的血管内皮细胞增殖和肿瘤新血管生成的生物学活性,其抗肿瘤作用具有高效、低毒、无耐药性的优点.……

HandPort手辅式腹腔镜大器官切除10例效益评估

目的 对采用HandPort手辅式腹腔镜大器官切除术的疗效及其效益进行评估。方法 在病人腹壁切一个5～6cm长的小切口,另置2个trocar(套管)。采用HandPort手辅式装置和超声刀,充分利用这个“小口”,运用开放手术和腹腔镜手术的技术,施行肝切除、结肠切除、空肠切除、脾切除和胃大部分切除等10例腹部大器官切除术。9例为择期手术,另1例结肠憩室大出血的病人急诊施行了右半结肠切除术。结果 10例手术时间为30′～2h,手术失血量为几毫升～80ml,中转开放手术率为0。病人术后过程平稳,痛苦小,进食早(术后1～2天),恢复快。结论 本方法在腹腔镜大器官切除术所需腹壁最小创伤的条件下,运用HandPort手辅式腹腔镜技术,在手术步骤上集中了传统开放手术和腹腔镜手术的优势,扬长避短,变难为易,使手术达到微创的目的。

全腔镜Ivor-Lewis术联合空肠造瘘术治疗中下段食管癌30例疗效观察

目的探讨全腔镜Ivor-Lewis术联合空肠造瘘术在临床上的应用及疗效。方法回顾性分析80例食管癌患者的临床资料。根据手术方式,分为全腔镜组(n=30)和小切口组(n=50)。全腔镜组采用全腔镜Ivor-Lewis术联合空肠造瘘术治疗方案;小切口组采用腹部小切口胸腹腔镜联合Ivor-Lewis术治疗方案。分别对比两组患者手术时间、术中失血量、置空肠营养管耗时、术后胸管引流量及胃肠减压管引流量、术后肺部感染、吻合口瘘等围手术期并发症发生例数、术后住院日数、术后疼痛评分、切口美容满意度等数据。结果全腔镜组手术耗时(242.00±18.69)min、术中失血量(153.83±33.37)m L、置空肠营养管耗时(14.20±3.47)min、住院日数(9.57±1.85)d、术后疼痛评分[24 h为(2.70±0.70)分,48 h为(2.10±0.71)分,72h为(1.73±0.83)分]、手术切口美容满意度均优于常规组[VAS得分为(81.53±9.35)分,Likert Scales得分为(3.93±0.785)分],均差异有统计学意义(P<0.05);两组患者在淋巴结清扫数目,术后胸管引流量及胃肠减压管引流量,二次开胸腹止血、吻合口瘘、胃壁坏死及胃残端瘘、乳糜胸、肺部感染及肺不张等并发症发生率方面,差异无统计学意义(P>0.05)。结论运用全腔镜食管癌根治术联合空肠造瘘术,保证了腹壁的完整性,手术切口瘢痕微小,术中出血减少,术后疼痛降低,有效缩短手术时间及治疗疗程,使得患者快速康复出院。

采用套索驱动的手部助力外骨骼设计与抓握实验研究

针对航天和工业领域中人体佩戴手套执行任务时,手指关节处因受到编织物挤压而造成的手部疲劳损伤以及抓握力下降等问题,设计了一种采用套索驱动的手部助力外骨骼系统。该系统采用以速度为内环、力为外环的力PID控制方法,以拉力传感器反馈输出力,通过力学建模对手部助力外骨骼导线槽开展位置优化。对所设计的外骨骼系统开展抓握实验,结果表明:人体穿戴手部助力外骨骼可再现Feix分类法中全部33种抓握类型;外骨骼抓握指尖力最高可达14N;与不穿戴外骨骼相比,穿戴外骨骼时人体手指关节运动范围能够达到自主弯曲运动范围的63%以上,掌指关节、近端指间关节和大拇指的指间关节角度的皮尔森相关系数可达0.90以上;外骨骼助力抓握使得肱桡肌和桡侧腕长伸肌的肌电均方根峰值分别减小了37.86%和62.46%。实验结果证明所设计的手部助力外骨骼能够明显减少手部肌肉耗能。

响应面优化伏毛铁棒锤总生物碱微波辅助提取工艺

目的:优化微波辅助提取伏毛铁棒锤总生物碱工艺,并比较不同产地伏毛铁棒锤中总生物碱含量。方法:以单因素试验为基础,结合响应面法,以总生物碱的含量为评价指标,考察温度、料液比、时间和体积分数对提取得率的影响。结果:最佳微波辅助提取工艺参数(条件)为:温度48℃,料液比1∶19(g/mL),时间12 min,甲醇体积分数39%。结论:该提取方法稳定可靠,操作简便,可用于优化伏毛铁棒锤总生物碱提取工艺。比较不同地区伏毛铁棒锤中总生物碱含量,贵南县伏毛铁棒锤总生物碱含量最高,为0.290%。

手助腹腔镜巨脾切除联合贲门食管血管离断术的临床疗效分析

目的:探讨手助腹腔镜巨脾切除联合贲门食管血管离断术的临床疗效。方法:回顾分析2015年1月至2023年1月为52例患者行脾切除联合贲门食管周围血管离断术的临床资料,根据手术方式分为腔镜组(行完全腹腔镜手术,n=18)、手助组(行手助腹腔镜手术,n=11)与开腹组(n=23),对比分析三组患者一般资料、手术疗效及术后并发症。结果:3组患者年龄、性别、入院血常规、Child-Pugh分级等一般资料差异无统计学意义(P>0.05)。手助组脾脏最大径大于腔镜组(P<0.001)。手助组手术时间、术后排气时间、术后住院时间短于开腹组(P<0.01),与腔镜组相比差异无统计学意义(P>0.05)。手助组术中出血量少于腔镜组与开腹组(P<0.01)。腔镜组、手助组及开腹组术后并发症发生率分别为16.7%、9.1%与34.8%,差异无统计学意义(P=0.182)。3组术后腹腔出血、胰瘘等并发症发生率差异无统计学意义(P>0.05)。结论:为巨型脾脏患者行手辅助腹腔镜脾切除联合贲门食管周围血管离断术不会增加手术风险及术后并发症,且具有创伤小、康复快等微创优势,值得临床推广。

单孔机器人辅助腹腔镜输尿管再植术与腹腔镜输尿管再植术治疗膀胱输尿管反流的对比研究

目的比较单孔机器人辅助腹腔镜输尿管再植术(robot-assisted laparoscopic ureteral reimplantation,RALUR)与腹腔镜输尿管再植术(laparoscopic ureteral reimplantation,LUR)治疗儿童原发性膀胱输尿管反流(vesicoureteral reflux,VUR)的疗效与应用。方法回顾性分析2017年1月至2022年4月于福建省立医院小儿外科行手术治疗的70例VUR患儿临床资料,根据手术方式将患儿分为机器人组(采取单孔机器人辅助腹腔镜输尿管再植术)及腹腔镜组(采取腹腔镜输尿管再植术)。机器人组30例,其中单侧反流22例,双侧反流8例;腹腔镜组40例,其中单侧反流30例,双侧反流10例。比较两组术前准备时间、腹腔操作时间、术中出血量、住院时间、手术成功率以及术后并发症发生情况等。结果两组均手术成功,无一例中转开放手术。手术时间:单侧机器人组(155.18±18.94)min,单侧腹腔镜组(126.33±19.75)min,差异有统计学意义(P<0.05);双侧机器人组(210.50±19.25)min,双侧腹腔镜组(177.10±16.61)min,差异有统计学意义(P<0.05)。手术操作时间:单侧机器人组(117.77±23.21)min,单侧腹腔镜组(109.30±10.57)min,差异无统计学意义(P=0.180);双侧机器人组(169.50±25.48)min,双侧腹腔镜组(158.50±16.67)min,差异无统计学意义(P=0.285)。术中出血量:机器人组(15.20±3.24)mL,腹腔镜组(25.23±5.84)mL,差异有统计学意义(P<0.05)。住院时间:机器人组6.5(6.0,7.0)d,腹腔镜组7.0(7.0,8.0)d,差异有统计学意义(P<0.05)。术后留置导尿管时间:机器人组3.0(3.0,4.0)d,腹腔镜组3.0(3.0,4.0)d,差异无统计学意义(P=0.714)。机器人组有1例、腹腔镜组有2例术后出现发热性泌尿系感染(urinary tract infection,UTI),经抗感染治疗后痊愈。机器人组有1例、腹腔镜组有3例术后出现单侧输尿管反流,经预防性口服抗生素治疗3个月后均反流消失。机器人组有1例、腹腔镜组有3例术后出现急性尿潴留,经延长留置导尿时间至术后2周,未再出现尿潴留。结论单孔机器人辅助腹腔镜输尿管再植术与腹腔镜下输尿管再植术治疗小儿VUR均安全有效,单孔RALUR相对于LUR手术时间长,然而术中出血量少,住院时间短,且单孔RALUR切口美观,具有一定的临床应用价值。