Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and P...Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and PacBio sequencing technique to assemble a high-quality,chromosome-level genome for this species.The 539 Mb genome had a contig N50 with a size of 3.43 Mb,while 755 contigs clustered into 24 chromosomal groups with an anchoring rate of 99.02%.Of the total genomic sequence,132.74Mb(24.39%)were annotated as repeat elements.A total of 21360 protein-coding genes were identified,of which 20787 genes(97.32%)were successfully annotated to public databases.The BUSCO evaluation indicated that 96.90%of the total orthologous genes were matched.The phylogenetic tree representing H.analis and 14 other bony fish species indicated that the H.analis genome contained 364 expanded gene families related to olfactory receptor activity,compared with the common ancestor of H.analis and Sciaenidae.Comparative genomic analysis further identified 3584 contracted gene families.Branch-site modeling identified 277 genes experiencing positive selection,which may facilitate the adaptation to rocky reef environments.The genome reported here is helpful for ecological and evolutionary studies of H.analis.展开更多
[ Objective] This study aimed to isolate and identify Vibrio ichthyoenteri strains from diseased Hapalogenys nitens. [ Method] In January 2013, dis- eased H. nitens juveniles from a maricultural farm in Luoyuanwan Bay...[ Objective] This study aimed to isolate and identify Vibrio ichthyoenteri strains from diseased Hapalogenys nitens. [ Method] In January 2013, dis- eased H. nitens juveniles from a maricultural farm in Luoyuanwan Bay of Fuzhou City were selected to investigate the incidence, clinical symptoms and pathogenic characteristics. Liver, spleen, kidney and gill of five diseased H. nitens juveniles were collected for bacterial isolation and pure culture to conduct morphological, physiological and biochemical assays, 16S rRNA gene sequencing and phylogenetic analysis. The susceptibility of five strains to 30 kinds of antimicrobial drugs was detected. [ Result] The death of H. nitens juveniles was caused by bacterial infections. The isolated strains fzlyw201301071 and fzlyw201301072 belonged to the same genus as Vibrio ichthyoenteri. Drug susceptibihty test suggested that there were no significant differences in drug sensitivity and resistance among different strains. [ Conclusion] This study provided theoretical basis for the prevention and control of diseases in aquaculture animals.展开更多
基金supported by the Province Key Research and Development Program of Zhejiang (No.2021C02047)the Special Projects of Zhejiang Provincial Science and Technology Department (Nos.HYS-CZ-004,HYS-CZ-202208)the‘San Nong Jiu Fang’Science and Technology Cooperation Project of Zhejiang Province (No.2022 SN JF073)。
文摘Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and PacBio sequencing technique to assemble a high-quality,chromosome-level genome for this species.The 539 Mb genome had a contig N50 with a size of 3.43 Mb,while 755 contigs clustered into 24 chromosomal groups with an anchoring rate of 99.02%.Of the total genomic sequence,132.74Mb(24.39%)were annotated as repeat elements.A total of 21360 protein-coding genes were identified,of which 20787 genes(97.32%)were successfully annotated to public databases.The BUSCO evaluation indicated that 96.90%of the total orthologous genes were matched.The phylogenetic tree representing H.analis and 14 other bony fish species indicated that the H.analis genome contained 364 expanded gene families related to olfactory receptor activity,compared with the common ancestor of H.analis and Sciaenidae.Comparative genomic analysis further identified 3584 contracted gene families.Branch-site modeling identified 277 genes experiencing positive selection,which may facilitate the adaptation to rocky reef environments.The genome reported here is helpful for ecological and evolutionary studies of H.analis.
基金Supported by Program of Aquatic Animal Disease Monitoring in Fujian Province(2012)Program of Fishery Pest Control in Fuzhou City(2012)
文摘[ Objective] This study aimed to isolate and identify Vibrio ichthyoenteri strains from diseased Hapalogenys nitens. [ Method] In January 2013, dis- eased H. nitens juveniles from a maricultural farm in Luoyuanwan Bay of Fuzhou City were selected to investigate the incidence, clinical symptoms and pathogenic characteristics. Liver, spleen, kidney and gill of five diseased H. nitens juveniles were collected for bacterial isolation and pure culture to conduct morphological, physiological and biochemical assays, 16S rRNA gene sequencing and phylogenetic analysis. The susceptibility of five strains to 30 kinds of antimicrobial drugs was detected. [ Result] The death of H. nitens juveniles was caused by bacterial infections. The isolated strains fzlyw201301071 and fzlyw201301072 belonged to the same genus as Vibrio ichthyoenteri. Drug susceptibihty test suggested that there were no significant differences in drug sensitivity and resistance among different strains. [ Conclusion] This study provided theoretical basis for the prevention and control of diseases in aquaculture animals.
