Objective: TO study the mechanism of cancer, theDNA for BAC was cloned from an ascites hepatoma cellline Hca-F25lCL-16A3 using PCR. Methods: The nucleotide sequences were determined using ABI PRISMTM377 DNA sequencer....Objective: TO study the mechanism of cancer, theDNA for BAC was cloned from an ascites hepatoma cellline Hca-F25lCL-16A3 using PCR. Methods: The nucleotide sequences were determined using ABI PRISMTM377 DNA sequencer. The expression of hcf-2 and CDK4gene were determined using im munohis tochemis try.Results: The sequences of BAC segment on Hca-F25lCL-16A3 have nearly identical sequences withhuman BAC. The hcl-2 and CDK4 are highly expressionon this cell line. Conclusion: The highly expression ofhcf-2 and CDK4 may the one of mechanisms for tumorgrowth.展开更多
To analyze immune response to murine hepatocarcinoma Hca-F of mice immunized with heat shock protein 70 （HSP70） derived from elemene combo tumor cell vaccine （EC-TCV） of Hca-F, HSP70 was isolated from EC-TCV by AD...To analyze immune response to murine hepatocarcinoma Hca-F of mice immunized with heat shock protein 70 （HSP70） derived from elemene combo tumor cell vaccine （EC-TCV） of Hca-F, HSP70 was isolated from EC-TCV by ADP affinity chromatography. Mice were immunized with HSP70 intraperitoneally three times and spleen calls were sampled. For cells, their proliferation and cytotoxicity against Hca-F were measured with MTT assay and their phenotypes were analyzed with flow cytometry. Spleen cells of immunized mice with HSP70 exhibited more potent cytotoxicity against Hca-F and proliferation than that of normal control mice, but less potent than that of mice immunized with EC-TCV. Among three groups, the percent of T6 T lymphocytes in the mice immunized with HSP70 （35.5%） was the highest compared with 6.25% in normal mice, and 28.4% in the mice immunized with EC-TCV. Immunization of HSP70 derived from EC-TCV could elicit potent immune response to Hca-F. HSP70 is one of elements inducing anti-tumor immune responses against Hca-F.展开更多
文摘Objective: TO study the mechanism of cancer, theDNA for BAC was cloned from an ascites hepatoma cellline Hca-F25lCL-16A3 using PCR. Methods: The nucleotide sequences were determined using ABI PRISMTM377 DNA sequencer. The expression of hcf-2 and CDK4gene were determined using im munohis tochemis try.Results: The sequences of BAC segment on Hca-F25lCL-16A3 have nearly identical sequences withhuman BAC. The hcl-2 and CDK4 are highly expressionon this cell line. Conclusion: The highly expression ofhcf-2 and CDK4 may the one of mechanisms for tumorgrowth.
基金supported by Key Programs of Science and Technology Research of Education Ministry of P.R.Chinathe National Science Foundation of China(39730440-11).
文摘To analyze immune response to murine hepatocarcinoma Hca-F of mice immunized with heat shock protein 70 （HSP70） derived from elemene combo tumor cell vaccine （EC-TCV） of Hca-F, HSP70 was isolated from EC-TCV by ADP affinity chromatography. Mice were immunized with HSP70 intraperitoneally three times and spleen calls were sampled. For cells, their proliferation and cytotoxicity against Hca-F were measured with MTT assay and their phenotypes were analyzed with flow cytometry. Spleen cells of immunized mice with HSP70 exhibited more potent cytotoxicity against Hca-F and proliferation than that of normal control mice, but less potent than that of mice immunized with EC-TCV. Among three groups, the percent of T6 T lymphocytes in the mice immunized with HSP70 （35.5%） was the highest compared with 6.25% in normal mice, and 28.4% in the mice immunized with EC-TCV. Immunization of HSP70 derived from EC-TCV could elicit potent immune response to Hca-F. HSP70 is one of elements inducing anti-tumor immune responses against Hca-F.
