The cult of Mazu is a common folk belief in China and Vietnam.Originating from the Song and Yuan Dynasties,particularly during the Ming and Qing Dynasties,the cult of Mazu spread to Vietnam through maritime trade and ...The cult of Mazu is a common folk belief in China and Vietnam.Originating from the Song and Yuan Dynasties,particularly during the Ming and Qing Dynasties,the cult of Mazu spread to Vietnam through maritime trade and migration from coastal regions of China.After centuries of integration and development,it evolved into its own cultural system of Mazu worship,becoming an important component of Vietnamese folk culture.Hainan,China,as a crucial supply station on the Maritime Silk Road,has close connections with the transmission of Mazu culture to Vietnam.This paper adopts field investigations and literature research methods to explore the Mazu worship and culture in Hainan,China,and the Qiongfu Assembly Halls in Vietnam.展开更多
This study aims to explore the integration of excellent traditional Chinese culture with ideological and political education in universities in Henan Province,focusing on the unique challenges and opportunities presen...This study aims to explore the integration of excellent traditional Chinese culture with ideological and political education in universities in Henan Province,focusing on the unique challenges and opportunities presented by the local context.Using a combination of quantitative and qualitative methods,the study analyzes the impact of cultural elements on political ideology and education in the region.The study contributes to a broader discussion of educational reform in China and provides insights for policymakers and educators seeking to develop well-rounded,culturally informed citizens.展开更多
This study focuses on Hong Kong youth,recognizing them as a vital subject within the context of“four histories”education.From the perspective of the integration of Lingnan culture,it explores the rich resources of L...This study focuses on Hong Kong youth,recognizing them as a vital subject within the context of“four histories”education.From the perspective of the integration of Lingnan culture,it explores the rich resources of Lingnan culture,innovates the narrative system,and explores the path of positive guidance in history education.Responding to the demands of the times,it seeks to alleviate the pressing concerns of Hong Kong society,help Hong Kong youth break down prejudices,gain a comprehensive understanding of China,grasp the development context of modern China,recognize historical trends,undertake national responsibilities,and take on the mission of the times.展开更多
A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol_producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the su...A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol_producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2α,5α,10β_triacetoxy_14β_propionyloxy_4(20),11_taxadiene (1), 2α,5α,10β_triacetoxy_14β_(2′_methyl)_butyryloxy_4(20),11_taxadiene (2), 2α,5α,10β_14β_tetra_acetoxy_4 (20),11_taxadiene (3, taxuyunnanine C), 2α,5α,10β_triacetoxy_14β_(2′_methyl_3′_hydroxy)_butyryloxy_4(20),11_taxadiene (4, yunnanxane) and its 3′_epimer (5), baccatin Ⅳ (6), baccatin Ⅲ (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large_scale culture.展开更多
As a specific region most abundant in bamboo species and their natural forests i n the world, China's Yunnan Province harbors over 250 bamboo species belongin g to 28 genera in its 331 000?hm\+2 of bamboo fore...As a specific region most abundant in bamboo species and their natural forests i n the world, China's Yunnan Province harbors over 250 bamboo species belongin g to 28 genera in its 331 000?hm\+2 of bamboo forests,thus forming a unique macro\| ba ckground of its ecotourist landscape resources.Not only is it acknowledged as on e of the original places and modern distribution centers of bamboo by scientists ,but it is a region with diverse ethnical and bamboo cultures nurtured out of th e peculiar ethnical customs and wide bamboo utilization of its 26 nationalities. Based on the analyses of the ornamental bamboo and its value in landscape resour ces,5 aesthetic characteristics are microscopically and morphologically approach ed as a material base of the formation of bamboo culture.By reexamining the macr o\|background of the specific natural and humanity landscape,4 categories are de scribed as fundamental macro\|landscape resources of Yunnan's bamboo culture eco tourism.After researching the ethnical ecological ethics and cultural symbolism, 3 typical ethnical traditions are summarized as traditional ecological awareness ,and 3 cultural features of bamboo are revealed as essence of ethnical bamboo cu lture preserved in Yunnan.Borrowing some theories from the new economic developm ent,innovation and successful ecotourist management experience at home and abroa d with wide academic vision,the authors have synthetically studied the substanti al,conceptual and symbolic landscapes in a broad field involving bamboo science, ethnology,ecology,ecoeconomics and tourist management.A preliminary theory of e thnical bamboo culture is thus constructed,and its peculiarities are briefly dis cussed while the correlation between ethnical bamboo culture and the natural res ources,other cultures as well as ecotourist development are systematically analy zed to lay a solid theoretical foundation and reliable scientific basis for ecoe conomy\|oriented forestry,cultural economics and ecotourist practice.Accordingly ,3 primary strategies and certain related principles are suggested for the susta inable development of ecotourism in Yunnan via effective protection and rational exploitation of abundant natural landscape resources and colorful humanity land scape resources including ethnical bamboo culture.展开更多
Chinese Root-kin Culture Expo-Park in Guishi County of Henan Province was studied,cultural background of the project and basic conditions of the site were introduced,planning concepts and supportive projects of the ro...Chinese Root-kin Culture Expo-Park in Guishi County of Henan Province was studied,cultural background of the project and basic conditions of the site were introduced,planning concepts and supportive projects of the root-kin culture theme park elaborated.The overall structure was designed to a pattern of "one axis and five subareas":one axis indicates the major sightseeing axis represented by Zhengxiao Riverfront Landscape Belt,and five subareas indicate Returning-to-homeland Park,Worshipping Ancestors Park,Folk Customs Park,Fujian and Taiwan Park,and Pioneering Park.Design concepts and landscape nodes of all subareas were introduced in detail,and Root-kin Culture applied in the integrated design.The authors proposed that landscape display based on Root-kin Culture should focus on materialization of cultures,reproduction of scenarios and creation of cultural atmosphere.Representative symbols should be refined from native cultures to create garden landscapes fit for actual conditions of the park.展开更多
To create a graceful living environment is the core responsibility of landscape designers in the designing process of residential environment. Residential environment design should pay attention to correctly involving...To create a graceful living environment is the core responsibility of landscape designers in the designing process of residential environment. Residential environment design should pay attention to correctly involving cultural feature and environmental and ecological elements which should be integrated in certain living environment through a good design method, so as to reflect landscape designers’ concern on the urban living space. Taking the buildings of Urban Paradise (Paradise in Chinese literature is also called Taohua Yuan, that is, Land of Peach Blossom, originated from the essay by TAO Yuan-ming in the Eastern Jin Dynasty) in Nanning City for example, the research investigated how to integrate ecology and culture in the environment creation of residential district.展开更多
Residential construction is the important part of new rural construction,also the great event relating to the living of the general farmers,but also the physical appearance of rural area.Taking advantage of the post-q...Residential construction is the important part of new rural construction,also the great event relating to the living of the general farmers,but also the physical appearance of rural area.Taking advantage of the post-quake reconstruction and new rural construction,combining with the inheritance and development of traditional culture,the new rural construction should be the first to be launched,constructing applicable and beautiful residences with rich residential cultural connotation.Longnan Region is economically underdeveloped,but it is with a beautiful environment and the forest coverage rate is high.Therefore,the discussion and investigation on the residential style and culture in this district are of great significance to the construction of the ethnic,economic and beautiful residential construction mode during new residential construction in Longnan Region.Through the analysis of the economic situation,environmental condition of Longnan Region and the analysis of the structure,function,architectural composition and existing problems of the present residence,the author discussed the style and structure of the present residence.The author also studied the protection and inheritance of the residential culture in Longnan Region,emphasizing the inheritance and development of residential culture and gave new cultural connotation to the new rural residential construction.All these provided reference for the new rural construction and planning in this area.展开更多
The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,an...The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.展开更多
Background: Gonocytes give rise to spermatogonial stem cells, and thereby play an essential role in establishing spermatogenesis. Optimized culture conditions for gonocytes provide an opportunity for their study and i...Background: Gonocytes give rise to spermatogonial stem cells, and thereby play an essential role in establishing spermatogenesis. Optimized culture conditions for gonocytes provide an opportunity for their study and in vitro manipulation for potential application in reproductive technologies. Using six experiments in a step-wise design, we examined the effects of several culture conditions on the maintenance, proliferation, and colony formation of porcine gonocytes. Testis cells from neonatal piglets were cultured for 7 d in DMEM supplemented with 10% fetal bovine serum. The examined culture conditions included using different cell seeding densities, gonocyte proportions, incubation temperatures, sampling strategies, and medium changing regimens.Results: Confluency of cel s was optimal(>90% by ~6 d) when 3.0 × 104 testis cel s/cm2 containing ~40% gonocytes were used. Incubating the cel s at 35 °C or 37 °C resulted in similar cel number and viability at confluency, but incubation at 35 °C resulted in a delayed confluency. In the first 2 d of culture, gonocytes remained mostly floating in the medium and gradual y settled over the next 5 d. Consequently, not changing the medium for 7 d(as opposed to changing it every 2 d) led to a significant increase in the number of gonocyte colonies by reducing the loss of "floating gonocytes".Conclusion: We found that gonocytes require the presence of a critical minimum number of somatic cel s for settlement, and can proliferate and form growing colonies even in a basic medium. Large numbers of viable gonocytes remain floating in the medium for several days. The optimized culture conditions in the present study included seeding with 3.0 × 104 testis cel s/cm2 containing ~40% gonocytes, incubating at 37 °C, and without changing the medium in the first week, which can result in improved colony formation of porcine gonocytes.展开更多
Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed...Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.展开更多
Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare pract...Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.展开更多
As the building of a socialist countryside in China is at a critical stage,village rules and regulations,as an important means of rural governance,is also in the orderly construction and operation. However,there still...As the building of a socialist countryside in China is at a critical stage,village rules and regulations,as an important means of rural governance,is also in the orderly construction and operation. However,there still exist obvious shortcomings in the contemporary rural governance,which hinders the active inheritance of the traditional villages' culture. Through the in-depth analysis of the relevant theories of rural governance and the historical culture of Ancient Zhentou Village,this study is expected to exploit the concept of the ancient village's culture in the heart of its dwellers so as to help them to better collect their existing cultural heritage.展开更多
Pre-modern Chinese crafts,such as iron smelting,cookery,medicine,and the production of vehicles,bows,and arrows indicate the traditional Chinese view of technology as being organic,holistic,and comprehensive.This view...Pre-modern Chinese crafts,such as iron smelting,cookery,medicine,and the production of vehicles,bows,and arrows indicate the traditional Chinese view of technology as being organic,holistic,and comprehensive.This view of technology is guided by the concept of he(和)and employs the means and methods of he,thus achieving the purport of he.In Chinese,the character he(和)possesses positive connotations.It originated from the meaning of"to season;to add flavoring to"(调和)and that of flavors being"perfectly harmonious"(和美).From this sensory experience,he gradually extended to the abstract levels of materiality,humanity,sociality,"order"(wei位),and "power,situation,force"(shi势).Finally,he was elevated to the supreme level of"qi of great harmony"(taihe zhi qi太和之气),which is comparable to the concept of dao(道).The philosophy of he has exerted wide impact on such areas as technology,art,national character,cultural psychology,and behavior patterns,and has become an integral part of China's inherent culture.The paradoxes and deviations of he hold their own profound philosophical implications that merit further exploration.As humanity confronts significant challenges,such as how we can coexist with others,with technology,and with nature,the ancient Eastern wisdom embodied in he continues to possess practical characteristics and value.展开更多
Regional culture of the Qinling Mountains shows distinct features since it was born in the local outstanding ecological environment, study on local architecture is significant for the dominant expression of regional c...Regional culture of the Qinling Mountains shows distinct features since it was born in the local outstanding ecological environment, study on local architecture is significant for the dominant expression of regional culture, protection of local environment, and echoing with the theme of ecological civilization construction. This paper, on the basis of the mutual infl uence and evolution of regional culture and style of local residential buildings, explored the reasons for the weakening of local architectural style, and specified the signifi cance of promoting local style of the living environment. By studying the infl uence of local natural environment and humanistic environment on architectural style along the northern foot of the Qinling Mountains, the paper explored the expression of regional culture in residential buildings, with Xian Garden(Xi'an Yuanzi) as an example, and aimed at giving useful help to the dominant expression of regional culture in modern residential buildings.展开更多
As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new cul...As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new culture modes were adopted to increase the production of Coscinodiscus sp.,the effect of changes in culture conditions and growth status on the hemostatic activity of diatoms was detected.To prevent Coscinodiscus sp.from sinking in culture,the suspension culture mode was realized by adding0.5%agar.The semi-continuous high nutrient concentration culture mode increased the cell density of Coscinodiscus sp.to 11000cells mL^(-1)and shorten the culture cycle to 5 d.In terms of coagulation activity,the addition of frustules reduced the in vitro coagulation time by half and the activation time of coagulation by 70%.The hemolysis rate and cytotoxicity of frustules harvested in the two culture modes did not change significantly.The results showed that suspension culture mode and high nutrient concentration culture mode only changed the growth state of Coscinodiscus sp.,while the hemostatic performance remained stable.展开更多
Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional agriculture.While most cellular agriculture is predominantly centered on the produc...Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional agriculture.While most cellular agriculture is predominantly centered on the production of cultured meat,there is a growing demand for an understanding of the production techniques involved in dairy products within cellular agriculture.This review focuses on the current status of cellular agriculture in the dairy sector and technical challenges for cell-cultured milk production.Cellular agriculture technology in the dairy sector has been classified into fermentation-based and animal cell culture-based cellular agriculture.Currently,various companies synthesize milk components through precision fermentation technology.Nevertheless,several startup companies are pursuing animal cell-based technology,driven by public concerns regarding genetically modified organisms in precision fermentation technology.Hence,this review offers an up-to-date exploration of animal cell-based cellular agriculture to produce milk components,specifically emphasizing the structural,functional,and productive aspects of mammary epithelial cells,providing new information for industry and academia.展开更多
The Woman Warrior" is an influential book in the 20th century making a thunder of voice in the world to accuse the stereotype of patriarchy and racism, which also shows the Chinese Americans’ difficulty of being...The Woman Warrior" is an influential book in the 20th century making a thunder of voice in the world to accuse the stereotype of patriarchy and racism, which also shows the Chinese Americans’ difficulty of being edged to the cultural cliff. This article analyses the content and the style of the novel to see inside what the writer wants to express and to figure out the real reason of the inequality between sexes and reveal the truth of the western culture dominance. Feminism, Patriarchy and culture are the main factors considered in the article.展开更多
Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible...Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.展开更多
Through exploring the material and nonmaterial cultural resources in leisure agriculture of Sani People in Shilin of Yunnan Province,this paper introduced festivals,marriage,traditional clothing,and food culture of Sa...Through exploring the material and nonmaterial cultural resources in leisure agriculture of Sani People in Shilin of Yunnan Province,this paper introduced festivals,marriage,traditional clothing,and food culture of Sani People,and present the long history and brilliant culture of Sani People. These culture resources could be used to expand the cultural aspects of leisure agriculture,to solve the problems of low participation and less distinct characteristics. This study can provide a reference for building the high-end leisure agriculture with distinct characteristics of Yunnan Province,to form a new growth point for regional growth.展开更多
基金sponsored by the 2021 Research Project of Hainan Research Center for Applied Foreign Languages(HNWYJD21-05)by the Scientific Research Project of Hainan Higher Education Institutions(Hnky2023-20).
文摘The cult of Mazu is a common folk belief in China and Vietnam.Originating from the Song and Yuan Dynasties,particularly during the Ming and Qing Dynasties,the cult of Mazu spread to Vietnam through maritime trade and migration from coastal regions of China.After centuries of integration and development,it evolved into its own cultural system of Mazu worship,becoming an important component of Vietnamese folk culture.Hainan,China,as a crucial supply station on the Maritime Silk Road,has close connections with the transmission of Mazu culture to Vietnam.This paper adopts field investigations and literature research methods to explore the Mazu worship and culture in Hainan,China,and the Qiongfu Assembly Halls in Vietnam.
文摘This study aims to explore the integration of excellent traditional Chinese culture with ideological and political education in universities in Henan Province,focusing on the unique challenges and opportunities presented by the local context.Using a combination of quantitative and qualitative methods,the study analyzes the impact of cultural elements on political ideology and education in the region.The study contributes to a broader discussion of educational reform in China and provides insights for policymakers and educators seeking to develop well-rounded,culturally informed citizens.
基金2021 Guangdong Provincial Education Science Planning Project(Moral Education Special)“Constructing Situation to Narrate:Exploring Lingnan Cultural Resources for Telling the China Story Well”(2021JKDY064)2021 Shenzhen Education Science Key Funding Project(14th Five-Year Plan)“Contradiction,Motivation,and Purpose:An Exploration of the Needs for a Better Life for Cross-Border Schoolchildren between Shenzhen and Hong Kong”(SZIIT2022SK037)。
文摘This study focuses on Hong Kong youth,recognizing them as a vital subject within the context of“four histories”education.From the perspective of the integration of Lingnan culture,it explores the rich resources of Lingnan culture,innovates the narrative system,and explores the path of positive guidance in history education.Responding to the demands of the times,it seeks to alleviate the pressing concerns of Hong Kong society,help Hong Kong youth break down prejudices,gain a comprehensive understanding of China,grasp the development context of modern China,recognize historical trends,undertake national responsibilities,and take on the mission of the times.
文摘A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol_producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2α,5α,10β_triacetoxy_14β_propionyloxy_4(20),11_taxadiene (1), 2α,5α,10β_triacetoxy_14β_(2′_methyl)_butyryloxy_4(20),11_taxadiene (2), 2α,5α,10β_14β_tetra_acetoxy_4 (20),11_taxadiene (3, taxuyunnanine C), 2α,5α,10β_triacetoxy_14β_(2′_methyl_3′_hydroxy)_butyryloxy_4(20),11_taxadiene (4, yunnanxane) and its 3′_epimer (5), baccatin Ⅳ (6), baccatin Ⅲ (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large_scale culture.
文摘As a specific region most abundant in bamboo species and their natural forests i n the world, China's Yunnan Province harbors over 250 bamboo species belongin g to 28 genera in its 331 000?hm\+2 of bamboo forests,thus forming a unique macro\| ba ckground of its ecotourist landscape resources.Not only is it acknowledged as on e of the original places and modern distribution centers of bamboo by scientists ,but it is a region with diverse ethnical and bamboo cultures nurtured out of th e peculiar ethnical customs and wide bamboo utilization of its 26 nationalities. Based on the analyses of the ornamental bamboo and its value in landscape resour ces,5 aesthetic characteristics are microscopically and morphologically approach ed as a material base of the formation of bamboo culture.By reexamining the macr o\|background of the specific natural and humanity landscape,4 categories are de scribed as fundamental macro\|landscape resources of Yunnan's bamboo culture eco tourism.After researching the ethnical ecological ethics and cultural symbolism, 3 typical ethnical traditions are summarized as traditional ecological awareness ,and 3 cultural features of bamboo are revealed as essence of ethnical bamboo cu lture preserved in Yunnan.Borrowing some theories from the new economic developm ent,innovation and successful ecotourist management experience at home and abroa d with wide academic vision,the authors have synthetically studied the substanti al,conceptual and symbolic landscapes in a broad field involving bamboo science, ethnology,ecology,ecoeconomics and tourist management.A preliminary theory of e thnical bamboo culture is thus constructed,and its peculiarities are briefly dis cussed while the correlation between ethnical bamboo culture and the natural res ources,other cultures as well as ecotourist development are systematically analy zed to lay a solid theoretical foundation and reliable scientific basis for ecoe conomy\|oriented forestry,cultural economics and ecotourist practice.Accordingly ,3 primary strategies and certain related principles are suggested for the susta inable development of ecotourism in Yunnan via effective protection and rational exploitation of abundant natural landscape resources and colorful humanity land scape resources including ethnical bamboo culture.
基金Supported by Municipal Administration and Financial Support Program of Gushi County~~
文摘Chinese Root-kin Culture Expo-Park in Guishi County of Henan Province was studied,cultural background of the project and basic conditions of the site were introduced,planning concepts and supportive projects of the root-kin culture theme park elaborated.The overall structure was designed to a pattern of "one axis and five subareas":one axis indicates the major sightseeing axis represented by Zhengxiao Riverfront Landscape Belt,and five subareas indicate Returning-to-homeland Park,Worshipping Ancestors Park,Folk Customs Park,Fujian and Taiwan Park,and Pioneering Park.Design concepts and landscape nodes of all subareas were introduced in detail,and Root-kin Culture applied in the integrated design.The authors proposed that landscape display based on Root-kin Culture should focus on materialization of cultures,reproduction of scenarios and creation of cultural atmosphere.Representative symbols should be refined from native cultures to create garden landscapes fit for actual conditions of the park.
文摘To create a graceful living environment is the core responsibility of landscape designers in the designing process of residential environment. Residential environment design should pay attention to correctly involving cultural feature and environmental and ecological elements which should be integrated in certain living environment through a good design method, so as to reflect landscape designers’ concern on the urban living space. Taking the buildings of Urban Paradise (Paradise in Chinese literature is also called Taohua Yuan, that is, Land of Peach Blossom, originated from the essay by TAO Yuan-ming in the Eastern Jin Dynasty) in Nanning City for example, the research investigated how to integrate ecology and culture in the environment creation of residential district.
文摘Residential construction is the important part of new rural construction,also the great event relating to the living of the general farmers,but also the physical appearance of rural area.Taking advantage of the post-quake reconstruction and new rural construction,combining with the inheritance and development of traditional culture,the new rural construction should be the first to be launched,constructing applicable and beautiful residences with rich residential cultural connotation.Longnan Region is economically underdeveloped,but it is with a beautiful environment and the forest coverage rate is high.Therefore,the discussion and investigation on the residential style and culture in this district are of great significance to the construction of the ethnic,economic and beautiful residential construction mode during new residential construction in Longnan Region.Through the analysis of the economic situation,environmental condition of Longnan Region and the analysis of the structure,function,architectural composition and existing problems of the present residence,the author discussed the style and structure of the present residence.The author also studied the protection and inheritance of the residential culture in Longnan Region,emphasizing the inheritance and development of residential culture and gave new cultural connotation to the new rural residential construction.All these provided reference for the new rural construction and planning in this area.
基金supported by the National Key Research and Development Program of China(2021YFD2100902-3)the National Natural Science Foundation of China(32072258)+5 种基金Major Science and Technology Program of Heilongjiang(2020ZX08B02)Harbin University of Commerce“Young Innovative Talents”Support Program(2019CX062020CX262020CX27)the Central Financial Support for the Development of Local Colleges and Universities,Graduate Innovation Research Project of Harbin University of Commerce(YJSCX2021-698HSD)Training plan of Young Innovative Talents in Universities of Heilongjiang(UNPYSCT-2020218).
文摘The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.
基金financially supported by grants from the Natural Science of Engineering Research and Council(NSERC)of Canada awarded to A.HonaramoozGraduate student scholarships to A.H.Awang-Junaidi were provided by the Ministry of Higher Education of Malaysia,the University of Saskatchewan College of Graduate and Postdoctoral Studiesthe University of Saskatchewan Western College of Veterinary Medicine
文摘Background: Gonocytes give rise to spermatogonial stem cells, and thereby play an essential role in establishing spermatogenesis. Optimized culture conditions for gonocytes provide an opportunity for their study and in vitro manipulation for potential application in reproductive technologies. Using six experiments in a step-wise design, we examined the effects of several culture conditions on the maintenance, proliferation, and colony formation of porcine gonocytes. Testis cells from neonatal piglets were cultured for 7 d in DMEM supplemented with 10% fetal bovine serum. The examined culture conditions included using different cell seeding densities, gonocyte proportions, incubation temperatures, sampling strategies, and medium changing regimens.Results: Confluency of cel s was optimal(>90% by ~6 d) when 3.0 × 104 testis cel s/cm2 containing ~40% gonocytes were used. Incubating the cel s at 35 °C or 37 °C resulted in similar cel number and viability at confluency, but incubation at 35 °C resulted in a delayed confluency. In the first 2 d of culture, gonocytes remained mostly floating in the medium and gradual y settled over the next 5 d. Consequently, not changing the medium for 7 d(as opposed to changing it every 2 d) led to a significant increase in the number of gonocyte colonies by reducing the loss of "floating gonocytes".Conclusion: We found that gonocytes require the presence of a critical minimum number of somatic cel s for settlement, and can proliferate and form growing colonies even in a basic medium. Large numbers of viable gonocytes remain floating in the medium for several days. The optimized culture conditions in the present study included seeding with 3.0 × 104 testis cel s/cm2 containing ~40% gonocytes, incubating at 37 °C, and without changing the medium in the first week, which can result in improved colony formation of porcine gonocytes.
基金partly funded by the Department of Science and Technology Fund for Improvement of S&T Infrastructure (Grant No. SR/FST/LS-I/2018/125)。
文摘Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.
文摘Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.
基金Sponsored by National Natural Science Foundation of China(51568025)
文摘As the building of a socialist countryside in China is at a critical stage,village rules and regulations,as an important means of rural governance,is also in the orderly construction and operation. However,there still exist obvious shortcomings in the contemporary rural governance,which hinders the active inheritance of the traditional villages' culture. Through the in-depth analysis of the relevant theories of rural governance and the historical culture of Ancient Zhentou Village,this study is expected to exploit the concept of the ancient village's culture in the heart of its dwellers so as to help them to better collect their existing cultural heritage.
基金the support of the Center for Cultural Studies on Science and Technology in China at Technische Universitat Berlin。
文摘Pre-modern Chinese crafts,such as iron smelting,cookery,medicine,and the production of vehicles,bows,and arrows indicate the traditional Chinese view of technology as being organic,holistic,and comprehensive.This view of technology is guided by the concept of he(和)and employs the means and methods of he,thus achieving the purport of he.In Chinese,the character he(和)possesses positive connotations.It originated from the meaning of"to season;to add flavoring to"(调和)and that of flavors being"perfectly harmonious"(和美).From this sensory experience,he gradually extended to the abstract levels of materiality,humanity,sociality,"order"(wei位),and "power,situation,force"(shi势).Finally,he was elevated to the supreme level of"qi of great harmony"(taihe zhi qi太和之气),which is comparable to the concept of dao(道).The philosophy of he has exerted wide impact on such areas as technology,art,national character,cultural psychology,and behavior patterns,and has become an integral part of China's inherent culture.The paradoxes and deviations of he hold their own profound philosophical implications that merit further exploration.As humanity confronts significant challenges,such as how we can coexist with others,with technology,and with nature,the ancient Eastern wisdom embodied in he continues to possess practical characteristics and value.
基金Sponsored by Key Research Projects of Humanistic and Social Sciences of Henan Provincial Department of Education(2013-ZD-002)Research Projects of Humanistic and Social Sciences of Henan Provincial Department of Education(2013-GH-141)
文摘Regional culture of the Qinling Mountains shows distinct features since it was born in the local outstanding ecological environment, study on local architecture is significant for the dominant expression of regional culture, protection of local environment, and echoing with the theme of ecological civilization construction. This paper, on the basis of the mutual infl uence and evolution of regional culture and style of local residential buildings, explored the reasons for the weakening of local architectural style, and specified the signifi cance of promoting local style of the living environment. By studying the infl uence of local natural environment and humanistic environment on architectural style along the northern foot of the Qinling Mountains, the paper explored the expression of regional culture in residential buildings, with Xian Garden(Xi'an Yuanzi) as an example, and aimed at giving useful help to the dominant expression of regional culture in modern residential buildings.
基金supported by the National Natural Science Foundation of China (No.U22A20588)the Sanya Science and Technology Project (No.2022KJCX57)+1 种基金the Qingdao National Laboratory for Marine Science and Technology (No.12-04)the Project supported by the Education Department of Hainan Province (No.Hnjg2024276)。
文摘As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new culture modes were adopted to increase the production of Coscinodiscus sp.,the effect of changes in culture conditions and growth status on the hemostatic activity of diatoms was detected.To prevent Coscinodiscus sp.from sinking in culture,the suspension culture mode was realized by adding0.5%agar.The semi-continuous high nutrient concentration culture mode increased the cell density of Coscinodiscus sp.to 11000cells mL^(-1)and shorten the culture cycle to 5 d.In terms of coagulation activity,the addition of frustules reduced the in vitro coagulation time by half and the activation time of coagulation by 70%.The hemolysis rate and cytotoxicity of frustules harvested in the two culture modes did not change significantly.The results showed that suspension culture mode and high nutrient concentration culture mode only changed the growth state of Coscinodiscus sp.,while the hemostatic performance remained stable.
基金supported by a National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)(No.2022R1A2C1008327)。
文摘Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional agriculture.While most cellular agriculture is predominantly centered on the production of cultured meat,there is a growing demand for an understanding of the production techniques involved in dairy products within cellular agriculture.This review focuses on the current status of cellular agriculture in the dairy sector and technical challenges for cell-cultured milk production.Cellular agriculture technology in the dairy sector has been classified into fermentation-based and animal cell culture-based cellular agriculture.Currently,various companies synthesize milk components through precision fermentation technology.Nevertheless,several startup companies are pursuing animal cell-based technology,driven by public concerns regarding genetically modified organisms in precision fermentation technology.Hence,this review offers an up-to-date exploration of animal cell-based cellular agriculture to produce milk components,specifically emphasizing the structural,functional,and productive aspects of mammary epithelial cells,providing new information for industry and academia.
文摘The Woman Warrior" is an influential book in the 20th century making a thunder of voice in the world to accuse the stereotype of patriarchy and racism, which also shows the Chinese Americans’ difficulty of being edged to the cultural cliff. This article analyses the content and the style of the novel to see inside what the writer wants to express and to figure out the real reason of the inequality between sexes and reveal the truth of the western culture dominance. Feminism, Patriarchy and culture are the main factors considered in the article.
基金support from the National Key Research and Development Program of China(Grant No.2017YFA0700501),and the Innovation Fund of WNLO.
文摘Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.
基金Supported by Science and Technology Project Research of Kunming University "Study on Yunnan Leisure Agriculture Cultural Origin"(XJL13021)Yunnan Province Urban Leisure Agriculture Engineering Technology Research Center Project "Study on Family Leisure Farm Circular Agriculture Development Model"(XXNY1410)+2 种基金Construction Project of Key Superior and Characteristic Disciplines(Ecology) in Colleges and Universities of Yunnan ProvinceScientific Research Foundation of Yunnan Provincial Department of Education "Study on Ecological Reconstruction of Old City Based on Low Impact Development Target"(2017ZZX090)Science and Technology Project Research of Kunming University "Study on LID System Theory Construction of Sponge City Landscape Corridor"(XJZD1602)
文摘Through exploring the material and nonmaterial cultural resources in leisure agriculture of Sani People in Shilin of Yunnan Province,this paper introduced festivals,marriage,traditional clothing,and food culture of Sani People,and present the long history and brilliant culture of Sani People. These culture resources could be used to expand the cultural aspects of leisure agriculture,to solve the problems of low participation and less distinct characteristics. This study can provide a reference for building the high-end leisure agriculture with distinct characteristics of Yunnan Province,to form a new growth point for regional growth.