Newcastle disease virus (NDV) is not considered as cause of serious disease in humans. But, recent data make it clear that, under particular circumstances, it is indeed possible for NDV to cause severe human respirato...Newcastle disease virus (NDV) is not considered as cause of serious disease in humans. But, recent data make it clear that, under particular circumstances, it is indeed possible for NDV to cause severe human respiratory disease. Newcastle Disease infection has been reported in many bird species. Cormorants that inhabit at the Qinghai-Tibet Plateau are mainly represented by Great Cormorant (Phalacrocorax carbo sinensis), which is distributed mainly on the Qinghai Lake area. Cormorants are considered as one of the main NDV-reservoir. We conducted the study for the presence of antibodies to Newcastle disease virus by hemagglutination inhibition test in yolks. We got 50% of seropositive yolks to Newcastle disease virus. These results show that NDV circulates in the Qinghai Lake population of cormorants. We first used the technique of detection of antibodies to Newcastle disease virus in the egg yolk for study the circulation of the virus in cormorants and demonstrated its effectiveness. We should carefully monitor cases of pneumonia in the population of people living around the lake and assess the causes of the disease.展开更多
In the poultry industry, it is common to evaluate the humoral immune response associated to the vaccination calendar used for the different zootechnical purposes. When the vaccine against avian influenza and Newcastle...In the poultry industry, it is common to evaluate the humoral immune response associated to the vaccination calendar used for the different zootechnical purposes. When the vaccine against avian influenza and Newcastle disease currently used in a farm is going to be changed, an important parameter observed to choose a product over the others is based on the antibody titers reached by the application of the new vaccine. This study aimed to compare the serologic results obtained by hemagglutination inhibition （HI） test of avian influenza and Newcastle disease reported in four different national laboratories. One-day-old Ross broiler chickens were kept in Horsfall-Bauer isolation units and were vaccinated subcutaneously for the prevention of avian influenza and Newcastle disease. Then, serum of all the birds was extracted at three, six and seven weeks old and sent to four different national diagnostic laboratories, where HI test was performed for avian influenza and Newcastle disease. The treatments were designed in 4 × 3 factorial. Data showed significant statistical differences between laboratory results （up to six logarithms for influenza at six and seven weeks）. This study confirms that the results of the HI test can vary from one laboratory to another, thus it is important to consider this, when the vaccines against avian influenza and Newcastle disease are evaluated at field.展开更多
Aluminum hydroxide sol （consisting of aluminum hydroxide nano-particles） prepared by a hydrothermal method was used as nano-aluminum adjuvant adsorbed the inactived Newcastle disease virus （NDV）. The zeta potentia...Aluminum hydroxide sol （consisting of aluminum hydroxide nano-particles） prepared by a hydrothermal method was used as nano-aluminum adjuvant adsorbed the inactived Newcastle disease virus （NDV）. The zeta potential of aluminum hydroxide colloidal particles,the inactivated NDV and the nano-aluminum adjuvant NDV are ＋48.16 mV,？13.8 mV and ＋39.97 mV,respec-tively. The nano-aluminum adjuvant vaccine was transparent and stable without precipitate,whereas the conventional aluminum ad-juvant vaccine was turbid and a white precipitate was visible soon. The hemagglutination inhibition （HI） titers of the nano-aluminum adjuvant group were generally higher than those of the conventional aluminum group except the titer on day 29. The results show that the nano-aluminum adjuvant has better stability and higher levels of antibodies for a longer time than the conventional aluminum adjuvant.展开更多
文摘Newcastle disease virus (NDV) is not considered as cause of serious disease in humans. But, recent data make it clear that, under particular circumstances, it is indeed possible for NDV to cause severe human respiratory disease. Newcastle Disease infection has been reported in many bird species. Cormorants that inhabit at the Qinghai-Tibet Plateau are mainly represented by Great Cormorant (Phalacrocorax carbo sinensis), which is distributed mainly on the Qinghai Lake area. Cormorants are considered as one of the main NDV-reservoir. We conducted the study for the presence of antibodies to Newcastle disease virus by hemagglutination inhibition test in yolks. We got 50% of seropositive yolks to Newcastle disease virus. These results show that NDV circulates in the Qinghai Lake population of cormorants. We first used the technique of detection of antibodies to Newcastle disease virus in the egg yolk for study the circulation of the virus in cormorants and demonstrated its effectiveness. We should carefully monitor cases of pneumonia in the population of people living around the lake and assess the causes of the disease.
文摘In the poultry industry, it is common to evaluate the humoral immune response associated to the vaccination calendar used for the different zootechnical purposes. When the vaccine against avian influenza and Newcastle disease currently used in a farm is going to be changed, an important parameter observed to choose a product over the others is based on the antibody titers reached by the application of the new vaccine. This study aimed to compare the serologic results obtained by hemagglutination inhibition （HI） test of avian influenza and Newcastle disease reported in four different national laboratories. One-day-old Ross broiler chickens were kept in Horsfall-Bauer isolation units and were vaccinated subcutaneously for the prevention of avian influenza and Newcastle disease. Then, serum of all the birds was extracted at three, six and seven weeks old and sent to four different national diagnostic laboratories, where HI test was performed for avian influenza and Newcastle disease. The treatments were designed in 4 × 3 factorial. Data showed significant statistical differences between laboratory results （up to six logarithms for influenza at six and seven weeks）. This study confirms that the results of the HI test can vary from one laboratory to another, thus it is important to consider this, when the vaccines against avian influenza and Newcastle disease are evaluated at field.
基金Supported by the National Science and Technology Supporting Program in the Eleventh Five-Year Plan of China (2006BAD06A08)
文摘Aluminum hydroxide sol （consisting of aluminum hydroxide nano-particles） prepared by a hydrothermal method was used as nano-aluminum adjuvant adsorbed the inactived Newcastle disease virus （NDV）. The zeta potential of aluminum hydroxide colloidal particles,the inactivated NDV and the nano-aluminum adjuvant NDV are ＋48.16 mV,？13.8 mV and ＋39.97 mV,respec-tively. The nano-aluminum adjuvant vaccine was transparent and stable without precipitate,whereas the conventional aluminum ad-juvant vaccine was turbid and a white precipitate was visible soon. The hemagglutination inhibition （HI） titers of the nano-aluminum adjuvant group were generally higher than those of the conventional aluminum group except the titer on day 29. The results show that the nano-aluminum adjuvant has better stability and higher levels of antibodies for a longer time than the conventional aluminum adjuvant.
