AIM To evaluate the one sampling and three sampling reverse passive hemagglutination fecal occult blood test (RPHA FOBT) for colorectal neoplasm screening.
In the poultry industry, it is common to evaluate the humoral immune response associated to the vaccination calendar used for the different zootechnical purposes. When the vaccine against avian influenza and Newcastle...In the poultry industry, it is common to evaluate the humoral immune response associated to the vaccination calendar used for the different zootechnical purposes. When the vaccine against avian influenza and Newcastle disease currently used in a farm is going to be changed, an important parameter observed to choose a product over the others is based on the antibody titers reached by the application of the new vaccine. This study aimed to compare the serologic results obtained by hemagglutination inhibition （HI） test of avian influenza and Newcastle disease reported in four different national laboratories. One-day-old Ross broiler chickens were kept in Horsfall-Bauer isolation units and were vaccinated subcutaneously for the prevention of avian influenza and Newcastle disease. Then, serum of all the birds was extracted at three, six and seven weeks old and sent to four different national diagnostic laboratories, where HI test was performed for avian influenza and Newcastle disease. The treatments were designed in 4 × 3 factorial. Data showed significant statistical differences between laboratory results （up to six logarithms for influenza at six and seven weeks）. This study confirms that the results of the HI test can vary from one laboratory to another, thus it is important to consider this, when the vaccines against avian influenza and Newcastle disease are evaluated at field.展开更多
In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-bindi...In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase （GST） fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A antigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.展开更多
文摘AIM To evaluate the one sampling and three sampling reverse passive hemagglutination fecal occult blood test (RPHA FOBT) for colorectal neoplasm screening.
文摘In the poultry industry, it is common to evaluate the humoral immune response associated to the vaccination calendar used for the different zootechnical purposes. When the vaccine against avian influenza and Newcastle disease currently used in a farm is going to be changed, an important parameter observed to choose a product over the others is based on the antibody titers reached by the application of the new vaccine. This study aimed to compare the serologic results obtained by hemagglutination inhibition （HI） test of avian influenza and Newcastle disease reported in four different national laboratories. One-day-old Ross broiler chickens were kept in Horsfall-Bauer isolation units and were vaccinated subcutaneously for the prevention of avian influenza and Newcastle disease. Then, serum of all the birds was extracted at three, six and seven weeks old and sent to four different national diagnostic laboratories, where HI test was performed for avian influenza and Newcastle disease. The treatments were designed in 4 × 3 factorial. Data showed significant statistical differences between laboratory results （up to six logarithms for influenza at six and seven weeks）. This study confirms that the results of the HI test can vary from one laboratory to another, thus it is important to consider this, when the vaccines against avian influenza and Newcastle disease are evaluated at field.
文摘In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase （GST） fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A antigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.
