[ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin （HE） staining method and obs...[ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin （HE） staining method and observed under microscope. Taenia solium specimens with clear structure and bright color were made. [ Results] Male reproductive system and excretory system of Taenia solium segment could be observed under the optical microscope, structures of uterus and eggs in Taenia solium gravid proglottid could also be observed. [ Conclusion] Aiming at providing a reference for the identification of Taenia solium and the diagnosis of the tapeworm disease.展开更多
Inflammation after stroke is the main cause of cerebral ischemia/reperfusion injury. Cascading events after injury can lead to cell death. Heat shock protein 70 and other endogenous injury-signaling molecules are rele...Inflammation after stroke is the main cause of cerebral ischemia/reperfusion injury. Cascading events after injury can lead to cell death. Heat shock protein 70 and other endogenous injury-signaling molecules are released by damaged cells, which can lead to systemic stress reactions. Protecting the brain through repair begins with the stress-injury-repair signaling chain. This study aimed to verify whether acupuncture acts through this chain to facilitate effective treatment of ischemic stroke. Rat models of cerebral ischemia/reperfusion injury were established by Zea Longa＇s method, and injury sites were identified by assessing neurological function, 2,3,5-triphenyltetrazolium chloride staining, and hematoxylin-eosin staining. Electroacupuncture at acupoints Baihui（DU20） and Zusanli（ST36） was performed in the model rats with dilatational waves, delivered for 20 minutes a day at 2–100 Hz and an amplitude of 2 m A. We analyzed the blood serum from the rats and found that inflammatory cytokines affected the levels of adrenotrophin and heat shock protein 70, each of which followed a similar bimodal curve. Specifically, electroacupuncture lowered the peak levels of adrenocorticotrophic hormone and heat shock protein 70. Thus, electroacupuncture was able to inhibit excessive stress, reduce inflammation, and promote the repair of neurons, which facilitated healing of ischemic stroke.展开更多
BACKGROUND Collagenous gastritis(CG) is a rare condition whose pathogenesis may be related to immune abnormalities. We report a case of CG from China.CASE SUMMARY A 24-year-old woman presented with recurrent abdominal...BACKGROUND Collagenous gastritis(CG) is a rare condition whose pathogenesis may be related to immune abnormalities. We report a case of CG from China.CASE SUMMARY A 24-year-old woman presented with recurrent abdominal distension and discomfort for 3 mo. Upper gastrointestinal endoscopy found diffuse nodular elevation-depression changes in the mucosa of the entire gastric corpus.Endoscopic ultrasound showed predominant involvement of the lamina propria and submucosa, and computed tomography imaging showed mild enhancement of the gastric wall. Pathological histology revealed that the thickness of the subepithelial collagen band was about 40 μm, and the Masson trichrome staining result was positive and the Congo red staining result was negative. This case is consistent with the child-adolescent type of CG.CONCLUSION Serum pepsinogen Ⅰ, pepsinogen Ⅱ, pepsinogen Ⅰ/Ⅱ ratio, and gastrin-17 may be potential non-invasive monitoring markers. Currently, treatments for CG vary,and the likely prognosis is unknown. Individual cases of gastric cancer in patients with CG have been reported.展开更多
Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast...Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast cancer cells in vitro,which can provide experimental ideas and methods for the establishment of a living tumor tissue cell bank.Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who needed surgical treatment in our hospital.Cells were isolated and used to establish successful cell culture.Cell activity and cell purity were measured before liquid nitrogen cryopreservation.Results(1)At the initial culture stage,cells grew with adherence.Cell multiplication could be seen after the cell medium was exchanged three times.Cell viability was above 86%,while the viability of the target cells was above 75%,as detected by hematoxylin and eosin(HE)staining.(2)The number of breast cancer cells decreased,while the number of fibroblasts increased after five rounds of passage.(3)The success rate was 73.08%,which did not include polluted cells and those that were not successfully cryopreserved.Conclusion(1)breast cancer cells could be selected from primary culture in vitro through an appropriate method.(2)Exchange of the cell medium and further cell passage improved cell multiplication.(3)The experimental results could be monitored using trypan blue and HE staining.(4)The success of breast cancer cell culture in vitro could be used as a reference for other cell culture,so as to establish a tumor tissue cell bank.展开更多
Background Treatment of rats with the beta-adrenergic agonist Isoprenaline（ISO） results in cardiac hypertrophy and myocardial fibrosis.In the present work,we aimed to study the in vivo effects of ISO on serum levels...Background Treatment of rats with the beta-adrenergic agonist Isoprenaline（ISO） results in cardiac hypertrophy and myocardial fibrosis.In the present work,we aimed to study the in vivo effects of ISO on serum levels of monocyte chemoattractant protein- 1 and tissue inhibitor of matrix metalloproteinases type I in Wistar rats.Methods ISO（5 mg·kg-1） or Saline were injected subcutaneously into Wistar rats once a day for 3 or 7 consecutive days.Ventricular remodeling and cardiac function were evaluated by echocardiography.Sections of heart were stained with hematoxylin-eosin（HE） for histopathology or with Massons trichrome for collagen visualization.In addition,heart tissue immunohistochemistry for ɑ-SMA was also analyzed.The serum levels of tissue inhibitor of matrix metalloproteinases type I（TIMP-1） and monocyte chemoattractant protein-1（MCP-1） were determined by Luminex multiplex technology.Results ISO induced cardiac dysfunction in rats after 3 or 7 days of treatment.ISO caused significant increase of myocardial disorder and fibrosis withincreased ɑ-SMA expression.ISO treated aats showed a significant increase in the serum levels of TIMP- 1 and MCP-1.Conclusions Our study suggests that ISO induces profound cardiac remodeling accompanied with increase of serum TIMP-1and MCP-1.展开更多
文摘[ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin （HE） staining method and observed under microscope. Taenia solium specimens with clear structure and bright color were made. [ Results] Male reproductive system and excretory system of Taenia solium segment could be observed under the optical microscope, structures of uterus and eggs in Taenia solium gravid proglottid could also be observed. [ Conclusion] Aiming at providing a reference for the identification of Taenia solium and the diagnosis of the tapeworm disease.
基金supported by a grant from the Major Science and Technology Project "Major New Drug Created" Funding,No.2009ZX09103-707
文摘Inflammation after stroke is the main cause of cerebral ischemia/reperfusion injury. Cascading events after injury can lead to cell death. Heat shock protein 70 and other endogenous injury-signaling molecules are released by damaged cells, which can lead to systemic stress reactions. Protecting the brain through repair begins with the stress-injury-repair signaling chain. This study aimed to verify whether acupuncture acts through this chain to facilitate effective treatment of ischemic stroke. Rat models of cerebral ischemia/reperfusion injury were established by Zea Longa＇s method, and injury sites were identified by assessing neurological function, 2,3,5-triphenyltetrazolium chloride staining, and hematoxylin-eosin staining. Electroacupuncture at acupoints Baihui（DU20） and Zusanli（ST36） was performed in the model rats with dilatational waves, delivered for 20 minutes a day at 2–100 Hz and an amplitude of 2 m A. We analyzed the blood serum from the rats and found that inflammatory cytokines affected the levels of adrenotrophin and heat shock protein 70, each of which followed a similar bimodal curve. Specifically, electroacupuncture lowered the peak levels of adrenocorticotrophic hormone and heat shock protein 70. Thus, electroacupuncture was able to inhibit excessive stress, reduce inflammation, and promote the repair of neurons, which facilitated healing of ischemic stroke.
文摘BACKGROUND Collagenous gastritis(CG) is a rare condition whose pathogenesis may be related to immune abnormalities. We report a case of CG from China.CASE SUMMARY A 24-year-old woman presented with recurrent abdominal distension and discomfort for 3 mo. Upper gastrointestinal endoscopy found diffuse nodular elevation-depression changes in the mucosa of the entire gastric corpus.Endoscopic ultrasound showed predominant involvement of the lamina propria and submucosa, and computed tomography imaging showed mild enhancement of the gastric wall. Pathological histology revealed that the thickness of the subepithelial collagen band was about 40 μm, and the Masson trichrome staining result was positive and the Congo red staining result was negative. This case is consistent with the child-adolescent type of CG.CONCLUSION Serum pepsinogen Ⅰ, pepsinogen Ⅱ, pepsinogen Ⅰ/Ⅱ ratio, and gastrin-17 may be potential non-invasive monitoring markers. Currently, treatments for CG vary,and the likely prognosis is unknown. Individual cases of gastric cancer in patients with CG have been reported.
基金a grant from the Science Foundation of Inner Mongolia Autonomous Region People’s Hospital(No.2016015).
文摘Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast cancer cells in vitro,which can provide experimental ideas and methods for the establishment of a living tumor tissue cell bank.Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who needed surgical treatment in our hospital.Cells were isolated and used to establish successful cell culture.Cell activity and cell purity were measured before liquid nitrogen cryopreservation.Results(1)At the initial culture stage,cells grew with adherence.Cell multiplication could be seen after the cell medium was exchanged three times.Cell viability was above 86%,while the viability of the target cells was above 75%,as detected by hematoxylin and eosin(HE)staining.(2)The number of breast cancer cells decreased,while the number of fibroblasts increased after five rounds of passage.(3)The success rate was 73.08%,which did not include polluted cells and those that were not successfully cryopreserved.Conclusion(1)breast cancer cells could be selected from primary culture in vitro through an appropriate method.(2)Exchange of the cell medium and further cell passage improved cell multiplication.(3)The experimental results could be monitored using trypan blue and HE staining.(4)The success of breast cancer cell culture in vitro could be used as a reference for other cell culture,so as to establish a tumor tissue cell bank.
基金supported by the National Natural Science Foundation of China(No.81202602/81373486)
文摘Background Treatment of rats with the beta-adrenergic agonist Isoprenaline（ISO） results in cardiac hypertrophy and myocardial fibrosis.In the present work,we aimed to study the in vivo effects of ISO on serum levels of monocyte chemoattractant protein- 1 and tissue inhibitor of matrix metalloproteinases type I in Wistar rats.Methods ISO（5 mg·kg-1） or Saline were injected subcutaneously into Wistar rats once a day for 3 or 7 consecutive days.Ventricular remodeling and cardiac function were evaluated by echocardiography.Sections of heart were stained with hematoxylin-eosin（HE） for histopathology or with Massons trichrome for collagen visualization.In addition,heart tissue immunohistochemistry for ɑ-SMA was also analyzed.The serum levels of tissue inhibitor of matrix metalloproteinases type I（TIMP-1） and monocyte chemoattractant protein-1（MCP-1） were determined by Luminex multiplex technology.Results ISO induced cardiac dysfunction in rats after 3 or 7 days of treatment.ISO caused significant increase of myocardial disorder and fibrosis withincreased ɑ-SMA expression.ISO treated aats showed a significant increase in the serum levels of TIMP- 1 and MCP-1.Conclusions Our study suggests that ISO induces profound cardiac remodeling accompanied with increase of serum TIMP-1and MCP-1.
