Hemo-parasites (HP) are one of the major constraints to the economic development of the livestock industry in Uganda. Generally, the occurrence and importance of HP is a reflection of complex interactions involving th...Hemo-parasites (HP) are one of the major constraints to the economic development of the livestock industry in Uganda. Generally, the occurrence and importance of HP is a reflection of complex interactions involving the causative organisms, tick vectors, the vertebrate hosts and the environment. We carried out a cross sectional study to identify and determine the prevalence of the major HPs in Central and Western Uganda, to form a baseline for appropriate interventions. A total of 295 bovine samples were analyzed from 15 districts of Uganda;56.3% being from the Central and 43.7% from the Western region of the country for a period of six months, and a questionnaire was administered to the farmers. Thin peripheral blood smears stained with Giemsa were used during the laboratory identification of the parasites. The disease prevalence was established at 47.4%, 6.7%, 1.9% and 14.4% for Theleria parva, Babesia spp., Trypanasoma brucei, Anaplasma spp. with a corresponding disease risk ratio (DRR) of 67.4%, 9.5%, 2.6%, 20.5% respectively in Uganda. The odds of having an infection from the Central region were 1.7 times greater (P < 0.05) than those from the Western region with a corresponding risk ratio of 1.2 (CI.1.1, 2.84, 95%), which showed that there exist marked differences between the two regions. Mean PCV was at 29.8%, and majority (59%) of infected animals had low PCV which varied across the months. These showed that generally, cattle in the West are more severely affected by HP than those from the Central region. The questionnaire revealed that 100% of the farmer treatments are based on clinical sign presentation, with occasional reliance on veterinarians. In conclusion, the burden of hemo-parasites is steadily increasing across the regions, and the current policies are not helping farmers who are hit the hardest by the development of antibiotic and acaricide resistance. These findings, along with previous studies, suggest that eradication HP is not feasible unless there are radical changes implemented, and that current practices are expensive and unsustainable. Further studies would be carried out to provide knowledge on the level of antibiotic resistance for the promotion of veterinary public health and trade.展开更多
This work described an amperometric hydrogen peroxide （H2O2） biosensor based on immobilization of hemoglobin （Hb） on a glassy carbon （GC） electrode modified by platinum nanoparticles, which was prepared by an in...This work described an amperometric hydrogen peroxide （H2O2） biosensor based on immobilization of hemoglobin （Hb） on a glassy carbon （GC） electrode modified by platinum nanoparticles, which was prepared by an in situ chemical reductive growth method. The electrochemical impedance measurements confirmed that the Hb was immobilized on the platinum nanoparticles-modified glassy carbon surface and has a synergistic effect with platinum nanoparti- cles in improving the catalytic reduction of H2O2. The Hb immobi- lized platinum nanoparticles-modified GC （Hb/Pt/GC） electrode displays an effective catalytic response to the reduction of H2O2. A linear dependence of the catalytic current versus H2O2 concentration was obtained in the range of 5.0×10 6 to 4.5×10^-4 mol·L^-1 with a detection limit （S/N=3） of 7.4×10^-7 mol·L-1.展开更多
文摘Hemo-parasites (HP) are one of the major constraints to the economic development of the livestock industry in Uganda. Generally, the occurrence and importance of HP is a reflection of complex interactions involving the causative organisms, tick vectors, the vertebrate hosts and the environment. We carried out a cross sectional study to identify and determine the prevalence of the major HPs in Central and Western Uganda, to form a baseline for appropriate interventions. A total of 295 bovine samples were analyzed from 15 districts of Uganda;56.3% being from the Central and 43.7% from the Western region of the country for a period of six months, and a questionnaire was administered to the farmers. Thin peripheral blood smears stained with Giemsa were used during the laboratory identification of the parasites. The disease prevalence was established at 47.4%, 6.7%, 1.9% and 14.4% for Theleria parva, Babesia spp., Trypanasoma brucei, Anaplasma spp. with a corresponding disease risk ratio (DRR) of 67.4%, 9.5%, 2.6%, 20.5% respectively in Uganda. The odds of having an infection from the Central region were 1.7 times greater (P < 0.05) than those from the Western region with a corresponding risk ratio of 1.2 (CI.1.1, 2.84, 95%), which showed that there exist marked differences between the two regions. Mean PCV was at 29.8%, and majority (59%) of infected animals had low PCV which varied across the months. These showed that generally, cattle in the West are more severely affected by HP than those from the Central region. The questionnaire revealed that 100% of the farmer treatments are based on clinical sign presentation, with occasional reliance on veterinarians. In conclusion, the burden of hemo-parasites is steadily increasing across the regions, and the current policies are not helping farmers who are hit the hardest by the development of antibiotic and acaricide resistance. These findings, along with previous studies, suggest that eradication HP is not feasible unless there are radical changes implemented, and that current practices are expensive and unsustainable. Further studies would be carried out to provide knowledge on the level of antibiotic resistance for the promotion of veterinary public health and trade.
基金Supported by Science Research Foundation of Huazhong University of Science and Technology (2006Z002A)
文摘This work described an amperometric hydrogen peroxide （H2O2） biosensor based on immobilization of hemoglobin （Hb） on a glassy carbon （GC） electrode modified by platinum nanoparticles, which was prepared by an in situ chemical reductive growth method. The electrochemical impedance measurements confirmed that the Hb was immobilized on the platinum nanoparticles-modified glassy carbon surface and has a synergistic effect with platinum nanoparti- cles in improving the catalytic reduction of H2O2. The Hb immobi- lized platinum nanoparticles-modified GC （Hb/Pt/GC） electrode displays an effective catalytic response to the reduction of H2O2. A linear dependence of the catalytic current versus H2O2 concentration was obtained in the range of 5.0×10 6 to 4.5×10^-4 mol·L^-1 with a detection limit （S/N=3） of 7.4×10^-7 mol·L-1.
