Six different indigenous plants were screened for antifeedant and insecticidal activity against fourth instar larvae of Epilachna beetle, Henosepilachna vigintioctopunctata, which is a severe pest on brinjal. Among th...Six different indigenous plants were screened for antifeedant and insecticidal activity against fourth instar larvae of Epilachna beetle, Henosepilachna vigintioctopunctata, which is a severe pest on brinjal. Among the plants screened, Achyranthes aspera showed higher activity against the selected pest. Ethyl acetate extracts of A. aspera showed higher antifeedant index and insecticidal activity against fourth instar larvae of H. vigintioctopunctata. Preliminary phytochemical analysis revealed that the presence of alkaloid and quinines in the ethyl acetate extracts indicate higher percentage of activities. Hence, it may suggest its use for controlling the vegetables insect pest, H. vigintioctopunctata.展开更多
Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify se...Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify sex-related genes.We recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 bp.We found 5002 genes highly expressed in males and 2179 genes highly expressed in females.Expression profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis.Using all unigenes as references,we identified 6657 putative simple sequence repeats.The transcriptome sequences and gene expression profiles of female and male H.vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.展开更多
文摘Six different indigenous plants were screened for antifeedant and insecticidal activity against fourth instar larvae of Epilachna beetle, Henosepilachna vigintioctopunctata, which is a severe pest on brinjal. Among the plants screened, Achyranthes aspera showed higher activity against the selected pest. Ethyl acetate extracts of A. aspera showed higher antifeedant index and insecticidal activity against fourth instar larvae of H. vigintioctopunctata. Preliminary phytochemical analysis revealed that the presence of alkaloid and quinines in the ethyl acetate extracts indicate higher percentage of activities. Hence, it may suggest its use for controlling the vegetables insect pest, H. vigintioctopunctata.
基金the National Key R and DProgram of China(Grant No.2017YFD0200900)the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2017).
文摘Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify sex-related genes.We recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 bp.We found 5002 genes highly expressed in males and 2179 genes highly expressed in females.Expression profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis.Using all unigenes as references,we identified 6657 putative simple sequence repeats.The transcriptome sequences and gene expression profiles of female and male H.vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.
