从鸡贫血病毒(CAV———ch icken anem ia virus)总DNA中克隆凋亡蛋白(Apoptin)基因片段,并与pcDNA3.1/CT-GFP-TOPO载体构建融合载体,转化E.coli.DH5α,筛选氨苄抗性阳性克隆并鉴定.以该融合载体转染Hep2细胞后,在倒置荧光显微镜下观察...从鸡贫血病毒(CAV———ch icken anem ia virus)总DNA中克隆凋亡蛋白(Apoptin)基因片段,并与pcDNA3.1/CT-GFP-TOPO载体构建融合载体,转化E.coli.DH5α,筛选氨苄抗性阳性克隆并鉴定.以该融合载体转染Hep2细胞后,在倒置荧光显微镜下观察到绿色荧光标记,同时观察到细胞核形态学呈凋亡特征变化,表明凋亡蛋白可以诱导Hep2细胞系发生凋亡.展开更多
In phylum Echinodermata, the family Holothuridae is distinguished by its capacity of bioactive compounds. Sea cu- cumber Holothuria atra is commonly known as the lollyfish. The antifimgal activity was detected using a...In phylum Echinodermata, the family Holothuridae is distinguished by its capacity of bioactive compounds. Sea cu- cumber Holothuria atra is commonly known as the lollyfish. The antifimgal activity was detected using agar well diffusion method against the various fungal strains such as Trichoderma viride, Aspergillus niger, Aspergillus flavis, Candida albicans and Penicillium chrysogenum. Relatively high antifungal activity was seen against Candida albicans at 100 μL-1 concentration of extracts. Zone of inhibition was measured at 18 mm of diameter. The anti-tumor activities were detected against the Vero and Hep2 cell lines using MTT assay. The cells were treated with H. atra extract at concentrations 0.078-10mg mL-1. The extract showed high proliferative activity against the Hep2 cells. The body wall extracts of sea cucumber （H. atra） showed effective antifungal and antitumor activities All these findings suggest that the extracts could be used for the development of drugs.展开更多
文摘从鸡贫血病毒(CAV———ch icken anem ia virus)总DNA中克隆凋亡蛋白(Apoptin)基因片段,并与pcDNA3.1/CT-GFP-TOPO载体构建融合载体,转化E.coli.DH5α,筛选氨苄抗性阳性克隆并鉴定.以该融合载体转染Hep2细胞后,在倒置荧光显微镜下观察到绿色荧光标记,同时观察到细胞核形态学呈凋亡特征变化,表明凋亡蛋白可以诱导Hep2细胞系发生凋亡.
文摘In phylum Echinodermata, the family Holothuridae is distinguished by its capacity of bioactive compounds. Sea cu- cumber Holothuria atra is commonly known as the lollyfish. The antifimgal activity was detected using agar well diffusion method against the various fungal strains such as Trichoderma viride, Aspergillus niger, Aspergillus flavis, Candida albicans and Penicillium chrysogenum. Relatively high antifungal activity was seen against Candida albicans at 100 μL-1 concentration of extracts. Zone of inhibition was measured at 18 mm of diameter. The anti-tumor activities were detected against the Vero and Hep2 cell lines using MTT assay. The cells were treated with H. atra extract at concentrations 0.078-10mg mL-1. The extract showed high proliferative activity against the Hep2 cells. The body wall extracts of sea cucumber （H. atra） showed effective antifungal and antitumor activities All these findings suggest that the extracts could be used for the development of drugs.
