基于Hill动力学与Michaelis-Menten方程,建立理论模型研究发状分裂相关增强子1(Hairy and enhancer of split 1,Hes1)调控蛋白激酶B(Protein Kinase B,AKT)-鼠双微体2(Murine Double Minute2,MDM2)-抗癌基因p53(p53)-第10号染色体缺失...基于Hill动力学与Michaelis-Menten方程,建立理论模型研究发状分裂相关增强子1(Hairy and enhancer of split 1,Hes1)调控蛋白激酶B(Protein Kinase B,AKT)-鼠双微体2(Murine Double Minute2,MDM2)-抗癌基因p53(p53)-第10号染色体缺失的磷酸酶及张力蛋白同源的基因(Phosphatase and tensin homolog deleted on chromosome ten,PTEN)通路的一种物理机制.研究发现,Hes1通过与PTEN结合抑制PTEN表达,并调控AKT信号.表明了Hes1蛋白的合成,以及Hes1与PTEN相互作用调控AKT-MDM2-p53-PTEN通路信号,将会有效地控制细胞结果.Hes1作为AKT-MDM2-p53-PTEN信号通路中上游调节的重要因素,还可以在一定程度上通过影响p53蛋白功能,改变p53对肿瘤的抑制性.理论结果可用于预测Notch通路信号异常诱导的致癌性,并进一步揭示了Notch信号通路影响细胞AKT-MDM2-p53-PTEN通路的激活机制.展开更多
Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial...Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.展开更多
目的观察丹龙醒脑方对脑缺血再灌注模型大鼠侧脑室室管膜下区(SVZ)神经干细胞(NSCs)增殖与Hes1、Hes5表达的影响,探讨其促进内源性NSCs增殖的作用机制。方法将80只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(依达组)、丹龙醒脑方...目的观察丹龙醒脑方对脑缺血再灌注模型大鼠侧脑室室管膜下区(SVZ)神经干细胞(NSCs)增殖与Hes1、Hes5表达的影响,探讨其促进内源性NSCs增殖的作用机制。方法将80只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(依达组)、丹龙醒脑方组(丹龙组)。采用线栓法制备局灶性脑缺血再灌注模型,再灌注7 d后取缺血侧SVZ脑组织。Brdu免疫荧光法检测SVZ NSCs增殖,RT-q PCR、Western blot分别检测Hes1、Hes5 m RNA和蛋白的表达。结果与假手术组比较,其余各组Brdu阳性细胞率增加,Hes1、Hes5 m RNA及蛋白表达明显升高(P<0.01);与模型组比较,依达组、丹龙组Brdu阳性细胞率明显增加,Hes1、Hes5 m RNA及蛋白表达水平明显增强(P<0.01);丹龙组Hes1 m RNA表达水平优于依达组(P<0.01),其余指标均无明显差异。结论丹龙醒脑方可促进脑缺血再灌注后大鼠SVZ NSCs增殖,并上调Hes1、Hes5表达水平,其机制可能与激活Notch信号通路有关。展开更多
文摘基于Hill动力学与Michaelis-Menten方程,建立理论模型研究发状分裂相关增强子1(Hairy and enhancer of split 1,Hes1)调控蛋白激酶B(Protein Kinase B,AKT)-鼠双微体2(Murine Double Minute2,MDM2)-抗癌基因p53(p53)-第10号染色体缺失的磷酸酶及张力蛋白同源的基因(Phosphatase and tensin homolog deleted on chromosome ten,PTEN)通路的一种物理机制.研究发现,Hes1通过与PTEN结合抑制PTEN表达,并调控AKT信号.表明了Hes1蛋白的合成,以及Hes1与PTEN相互作用调控AKT-MDM2-p53-PTEN通路信号,将会有效地控制细胞结果.Hes1作为AKT-MDM2-p53-PTEN信号通路中上游调节的重要因素,还可以在一定程度上通过影响p53蛋白功能,改变p53对肿瘤的抑制性.理论结果可用于预测Notch通路信号异常诱导的致癌性,并进一步揭示了Notch信号通路影响细胞AKT-MDM2-p53-PTEN通路的激活机制.
基金Program of Natural Science Foundation of Shanghai,Grant/Award Number:21ZR1453800 and 22ZR1452400Program of National Natural Science Foundation of China,Grant/Award Number:82370057+3 种基金Fundamental Research Funds for the Central Universities,Grant/Award Number:22120220562Program of Shanghai Municipal Health Commission,Grant/Award Number:20204Y0384Program of National Key Research and Development Project of China,Grant/Award Number:2023YFC2509500。
文摘Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.
文摘目的观察丹龙醒脑方对脑缺血再灌注模型大鼠侧脑室室管膜下区(SVZ)神经干细胞(NSCs)增殖与Hes1、Hes5表达的影响,探讨其促进内源性NSCs增殖的作用机制。方法将80只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(依达组)、丹龙醒脑方组(丹龙组)。采用线栓法制备局灶性脑缺血再灌注模型,再灌注7 d后取缺血侧SVZ脑组织。Brdu免疫荧光法检测SVZ NSCs增殖,RT-q PCR、Western blot分别检测Hes1、Hes5 m RNA和蛋白的表达。结果与假手术组比较,其余各组Brdu阳性细胞率增加,Hes1、Hes5 m RNA及蛋白表达明显升高(P<0.01);与模型组比较,依达组、丹龙组Brdu阳性细胞率明显增加,Hes1、Hes5 m RNA及蛋白表达水平明显增强(P<0.01);丹龙组Hes1 m RNA表达水平优于依达组(P<0.01),其余指标均无明显差异。结论丹龙醒脑方可促进脑缺血再灌注后大鼠SVZ NSCs增殖,并上调Hes1、Hes5表达水平,其机制可能与激活Notch信号通路有关。
