This paper investigates behaviors that a set of initial cell densities of Heterocapsa Circularisquama(HC)and Proroce nt rum Dentatum(PD)and a set of environmental factors disturbing the growth of HC and PD cells forme...This paper investigates behaviors that a set of initial cell densities of Heterocapsa Circularisquama(HC)and Proroce nt rum Dentatum(PD)and a set of environmental factors disturbing the growth of HC and PD cells formed in their growth process,which are actually fractal phenomena.First,the calculation of the fractal dimension of the set of initial cell densities of HC and PD is given in the paper.Second,by controlling the set of initial cell density,HC and PD cells grow according to a given growth target.The approximate or same behaviors of two different sets of initial cell densities of HC and PD are realized by introducing the coupling terms.Finally,the set of environmental factors distu rbing the growth of HC and PD cells is construe ted by introducing real parameters.Then the result that makes HC and PD cells growing according to a given growth target is reached by a proper mathematical transform to the real parameters.The approximate or same behaviors of the set of environmental factors disturbing the growth of HC and PD cells are realized by introducing the coupling terms.展开更多
Heterocapsa circularisquama RNA virus(HcRNAV) is the first single-stranded RNA virus to be characterized that infects dinoflagellates.The ability of HcRNAV coat protein(HcRNAV CP) to self-assemble into virus-like part...Heterocapsa circularisquama RNA virus(HcRNAV) is the first single-stranded RNA virus to be characterized that infects dinoflagellates.The ability of HcRNAV coat protein(HcRNAV CP) to self-assemble into virus-like particles(VLPs) in vitro suggested that heterologous expression was possible,and that the VLPs might be ideal nanocontainers for the targeted delivery of genes and chemicals.In this paper,we report the expression of a codon-optimized HcRNAV 109 CP gene in Pichia pastoris and the production of self-assembled HcRNAV VLPs using large-scale fermentation.The HcRNAV 109 CP gene was synthesized according to the codon preference of P.pastoris and cloned into a pPICZA vector.The recombinant plasmid pPICZA-CPsyns was transformed into P.pastoris by electroporation.The resulting yeast colonies were screened by PCR and analyzed for protein expression by SDS polyacrylamide gel electrophoresis.After large-scale fermentation,the yield of HcRNAV CPsyns reached approximately 2.5 g L 1 within 4 d.The HcRNAV VLPs were purified using PEG precipitation followed by cesium chloride density gradient ultracentrifugation,and were subsequently analyzed using UV spectrophotometry and transmission electron microscopy.Fluorescence dye-labeled myoglobin was loaded into the cages of the HcRNAV VLPs and the encapsulation was confirmed by fluorescence spectroscopy.The results point to the possible utilization in pharmacology or nanotechnology of HcRNAV VLPs produced by P.pastoris fermentation.展开更多
基金the National Natural Science Foundation of China(Nos.U1806203 and 61533011).
文摘This paper investigates behaviors that a set of initial cell densities of Heterocapsa Circularisquama(HC)and Proroce nt rum Dentatum(PD)and a set of environmental factors disturbing the growth of HC and PD cells formed in their growth process,which are actually fractal phenomena.First,the calculation of the fractal dimension of the set of initial cell densities of HC and PD is given in the paper.Second,by controlling the set of initial cell density,HC and PD cells grow according to a given growth target.The approximate or same behaviors of two different sets of initial cell densities of HC and PD are realized by introducing the coupling terms.Finally,the set of environmental factors distu rbing the growth of HC and PD cells is construe ted by introducing real parameters.Then the result that makes HC and PD cells growing according to a given growth target is reached by a proper mathematical transform to the real parameters.The approximate or same behaviors of the set of environmental factors disturbing the growth of HC and PD cells are realized by introducing the coupling terms.
基金supported by the Pioneer Research Center Program through the National Research Program of Korea funded by the Ministry of Education,Science and Technology,Korea (Grant M1071118001-08M1118-00110)(2010)
文摘Heterocapsa circularisquama RNA virus(HcRNAV) is the first single-stranded RNA virus to be characterized that infects dinoflagellates.The ability of HcRNAV coat protein(HcRNAV CP) to self-assemble into virus-like particles(VLPs) in vitro suggested that heterologous expression was possible,and that the VLPs might be ideal nanocontainers for the targeted delivery of genes and chemicals.In this paper,we report the expression of a codon-optimized HcRNAV 109 CP gene in Pichia pastoris and the production of self-assembled HcRNAV VLPs using large-scale fermentation.The HcRNAV 109 CP gene was synthesized according to the codon preference of P.pastoris and cloned into a pPICZA vector.The recombinant plasmid pPICZA-CPsyns was transformed into P.pastoris by electroporation.The resulting yeast colonies were screened by PCR and analyzed for protein expression by SDS polyacrylamide gel electrophoresis.After large-scale fermentation,the yield of HcRNAV CPsyns reached approximately 2.5 g L 1 within 4 d.The HcRNAV VLPs were purified using PEG precipitation followed by cesium chloride density gradient ultracentrifugation,and were subsequently analyzed using UV spectrophotometry and transmission electron microscopy.Fluorescence dye-labeled myoglobin was loaded into the cages of the HcRNAV VLPs and the encapsulation was confirmed by fluorescence spectroscopy.The results point to the possible utilization in pharmacology or nanotechnology of HcRNAV VLPs produced by P.pastoris fermentation.
