The effects of cadmium(Cd)on metal accumulation,microelements contents,and antioxidant responses in Hexagram-mos otakii were studied.The fish were exposed to 0.2,2.5,or 10μg L−1 Cd for 12 or 24 days.Then,the concentr...The effects of cadmium(Cd)on metal accumulation,microelements contents,and antioxidant responses in Hexagram-mos otakii were studied.The fish were exposed to 0.2,2.5,or 10μg L−1 Cd for 12 or 24 days.Then,the concentration of Cd and mi-croelements(Ca,Fe,Zn,and Se)were determined in the liver and kidney.Moreover,the activities of antioxidant enzymes including superoxide dismutase(SOD),catalase(CAT),and glutathione-S-transferase(GST),and the content of malondialdehyde(MDA)in the liver and kidney were also measured.A continuous accumulation of Cd was observed throughout the experimental period.Cd accumulation was higher in liver than that in the kidney,while Ca and Fe contents were lower in liver than those in the kidney.Cd exposure resulted in a decrease of Ca and Fe concentrations in the kidney,while there was no effect on the liver.Zn and Se remained unaffected with exposure to Cd.Cd exposure induced severe oxidative stress in H.otakii,as indicated by significant induction of the activities of SOD,CAT,and GST,and a simultaneous increase of MDA content.These data show that antioxidant enzymes and mi-croelements contents can be used as potential biomarkers to monitor environmental health in fish.展开更多
Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it...Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of H. otakii. In this study, 35 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1875 to 0.6562 and 0.2679 to 0.5079, respectively. The minor allele frequency ranged from 0.1875 to 0.4375. Polymorphic information content ranged from 0.229 to 0.375. Six SNPs were found to be deviated significantly from the HWE (P H. otakii.展开更多
Integrated multi-tropic aquaculture(IMTA)systems have been used in China for many years and have achieved significant economic,social,and ecological benefits.However,there is still a lack of benthic bioremediation spe...Integrated multi-tropic aquaculture(IMTA)systems have been used in China for many years and have achieved significant economic,social,and ecological benefits.However,there is still a lack of benthic bioremediation species that can effectively utilize the aquaculture particulate organic waste in the system.Polychaete Perinereis aibuhitensis Grube is used as an environmental remediation species for large-scale aquaculture to reduce particulate organic waste,which is of great significance to environmental protection.To improve bio-elements utilization efficiency,P.aibuhitensis was applied for IMTA indoor fish(Hexagrammos otakii)farming.Results showed that in the system,production of 1 kg of the fish discharged 2141-2338 mg of carbon and 529-532 mg of nitrogen,while in the monoculture of the fish,the figures were 3033-3390 mg and 764-794 mg,or 24.84%-35.26%and 30.35%-33.32%less,respectively.This approach promoted IMTA technology that could utilize the particulate organic waste from intensive aquaculture and reduce the adverse environmental effects.展开更多
基金supported by the Key Technology Re-search and Development Program of Shandong Province(Nos.2019GHY112071 and 2019GHY112062)the Major Agricultural Application Technology Innovation Pro-jects in Shandong Province(No.SD2019YY007).
文摘The effects of cadmium(Cd)on metal accumulation,microelements contents,and antioxidant responses in Hexagram-mos otakii were studied.The fish were exposed to 0.2,2.5,or 10μg L−1 Cd for 12 or 24 days.Then,the concentration of Cd and mi-croelements(Ca,Fe,Zn,and Se)were determined in the liver and kidney.Moreover,the activities of antioxidant enzymes including superoxide dismutase(SOD),catalase(CAT),and glutathione-S-transferase(GST),and the content of malondialdehyde(MDA)in the liver and kidney were also measured.A continuous accumulation of Cd was observed throughout the experimental period.Cd accumulation was higher in liver than that in the kidney,while Ca and Fe contents were lower in liver than those in the kidney.Cd exposure resulted in a decrease of Ca and Fe concentrations in the kidney,while there was no effect on the liver.Zn and Se remained unaffected with exposure to Cd.Cd exposure induced severe oxidative stress in H.otakii,as indicated by significant induction of the activities of SOD,CAT,and GST,and a simultaneous increase of MDA content.These data show that antioxidant enzymes and mi-croelements contents can be used as potential biomarkers to monitor environmental health in fish.
文摘Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of H. otakii. In this study, 35 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1875 to 0.6562 and 0.2679 to 0.5079, respectively. The minor allele frequency ranged from 0.1875 to 0.4375. Polymorphic information content ranged from 0.229 to 0.375. Six SNPs were found to be deviated significantly from the HWE (P H. otakii.
基金Supported by the National Natural Science Foundation of China(No.41876185)the Major Agricultural Applied Technological Innovation program in Shandong Province(No.SD2019YY007)the Central Public-interest Scientifi c Institution Basal Research Fund,Chinese Academy of Fishery Sciences(No.2018GH15)。
文摘Integrated multi-tropic aquaculture(IMTA)systems have been used in China for many years and have achieved significant economic,social,and ecological benefits.However,there is still a lack of benthic bioremediation species that can effectively utilize the aquaculture particulate organic waste in the system.Polychaete Perinereis aibuhitensis Grube is used as an environmental remediation species for large-scale aquaculture to reduce particulate organic waste,which is of great significance to environmental protection.To improve bio-elements utilization efficiency,P.aibuhitensis was applied for IMTA indoor fish(Hexagrammos otakii)farming.Results showed that in the system,production of 1 kg of the fish discharged 2141-2338 mg of carbon and 529-532 mg of nitrogen,while in the monoculture of the fish,the figures were 3033-3390 mg and 764-794 mg,or 24.84%-35.26%and 30.35%-33.32%less,respectively.This approach promoted IMTA technology that could utilize the particulate organic waste from intensive aquaculture and reduce the adverse environmental effects.
