Objective: To explore the clinical significance of serum uric acid combined with cystatin C detection in high risk degree of hypertension of different grades. Methods: The patients who were treated in the Department o...Objective: To explore the clinical significance of serum uric acid combined with cystatin C detection in high risk degree of hypertension of different grades. Methods: The patients who were treated in the Department of Cardiovascular Medicine of our hospital from January to December 2022 were selected as the study subjects, and the high risk and extremely high risk groups of grade I, II and III hypertension were selected for comparative analysis to explore their clinical significance. Results: There was a statistically significant difference in the number of patients with extremely high risk of grade II hypertension between men and women (P 0.05), which showed that there was no specificity in the concentration detection of serum uric acid and cystatin C in different grades of hypertension;there was no significant difference in serum uric acid concentration between grade II and grade III of hypertension with high risk grade and cystatin C concentration between grade I and grade III of hypertension with high risk grade (P > 0.05), but there was significant difference in the concentration detection of serum uric acid and cystatin C between the other groups (P < 0.05), which indicates that the detection of serum uric acid and cystatin C has important clinical significance in the high risk degree of hypertension. Conclusion: In the comparison of the detection of blood uric acid and cystatin C in different levels of hypertension with extremely high risk, the difference of cystatin C in grade I and grade III was not statistically significant, and the rest were statistically significant;therefore, the detection of serum uric acid and cystatin C can provide reliable laboratory data for clinical diagnosis and treatment of the extremely high risk degree of different levels of hypertension.展开更多
The authors conducted a retrospective study over a period of 6 months in a hypertensive population in order to determine the correlation between serum uric acid on glomerular filtration rate (GFR) and estimated serum ...The authors conducted a retrospective study over a period of 6 months in a hypertensive population in order to determine the correlation between serum uric acid on glomerular filtration rate (GFR) and estimated serum potassium with left ventricular hypertrophy (LVH) and obesity. This study enrolled 122 patients including 63 women (51.6%). The mean age was 55.9 ± 10.6 years (range 30 to 74 years). Obesity weight was found in 38 cases (31.1%) of which 20?were men (33.9%) and 18 women (28.6%). Abdominal obesity was found in 104 cases (85.2%). The average serum uric acid in patients with obesity weight was 63.3 ± 18.9 mg/L vs 62.4 ± 14.2 mg/L for no-obese patients (p?= 0.63). The average serum potassium in obese patients was 4.06 ± 0.42 mEq/L vs 4.02 ± 0.46 mEq/L for no-obese (p?= 0.65). The average GFR was 73.4 ± 21.4 ml/L in obese patients vs 66.6 ± 22.6 ml/min in no-obese (p?= 0.03). The LVH was found in 81 cases (66.4%). The LVH was found in 65 (62.5%) obese patients vs?16 (88%) non-obese patients (OR = 4.8, 95% 1.04?-?22?p?= 0.02). Only abdominal?obesity has been correlated with left ventricular hypertrophy after multivariate analysis. Emphasis must be focused on public health actions for effective and appropriate measures against obesity and hypertension, whose prevalence is increasing in our region.展开更多
Objective To investigate the effect of long-term and low-level occupational Mn exposure on the level of uric acid （UA） in human urine. Methods In this study, 65 volunteers were recruited, who were working on welding...Objective To investigate the effect of long-term and low-level occupational Mn exposure on the level of uric acid （UA） in human urine. Methods In this study, 65 volunteers were recruited, who were working on welding and foundry work in an plant in Gansu province, China. Additionally, 29 control samples were collected from individuals who did not have any history of excessive Mn exposure. An improved high performance liquid chromatography system equipped with a diode-array detector （HPLC-DAD） method was developed to determine the UA level in human urine. A Spectra AA 220 Atomic Absorption Spectrophotometer （AAS） was used to measure the Mn level in the urine. Results The analytical method was validated acceptable accuracy, precision, and recovery significantly lower than that of control samples for concentrations ranging from 3.82-45.84 pg/mL with Overall, the UA levels of Mn exposure samples were （P〈O.O5）. Conclusion The practical method developed here is suitable for both routine monitoring of UA level in human urine and metabolism research. Long-term and low-level occupational Mn exposure may lead to a lower UA level in urine, and UA might be an indicator of the early stage of manganism.展开更多
目的探索脊柱退变住院患者血清尿酸/高密度脂蛋白比值与骨密度的相关性。方法共纳入803例脊柱退变的受试者,评估临床因素及实验室检查结果,测量骨密度,按照骨密度结果分为骨质疏松症组及非骨质疏松症组。采用多元Logistic回归分析血清尿...目的探索脊柱退变住院患者血清尿酸/高密度脂蛋白比值与骨密度的相关性。方法共纳入803例脊柱退变的受试者,评估临床因素及实验室检查结果,测量骨密度,按照骨密度结果分为骨质疏松症组及非骨质疏松症组。采用多元Logistic回归分析血清尿酸/高密度脂蛋白比值(uric acid to high-density lipoprotein cholesterol ratio,UHR)与骨质疏松症的相关性。结果与非骨质疏松症组相比,骨质疏松症组的UHR更低(244.93±.102.51 vs 199.97±.91.96,P<0.001),多元Logistics回归分析提示,在校正了骨质疏松症的传统危险因素后,UHR最高的四分位患者发生骨质疏松症的可能性是UHR最低的四分位患者的0.402倍(P=0.018)。骨质疏松症的患病率在UHR四分位呈下降的趋势,骨密度在UHR四分位呈升高的趋势。UHR在骨量正常、骨量减少及骨质疏松症3组呈下降的趋势。结论低UHR是昆山地区脊柱退变住院患者发生骨质疏松症的危险因素。对于UHR较低的脊柱退变患者,应注意筛查骨质疏松症。展开更多
目的:采用两样本孟德尔随机化(Mendelian Randomization,MR)方法,探讨高尿酸与房颤之间的因果关系。方法:从IEU Open GWAS数据库中检索单核苷酸多态性(SNP)数量最多的文献数据,将高尿酸作为暴露因素,将房颤作为结局变量。采用MR-Egger...目的:采用两样本孟德尔随机化(Mendelian Randomization,MR)方法,探讨高尿酸与房颤之间的因果关系。方法:从IEU Open GWAS数据库中检索单核苷酸多态性(SNP)数量最多的文献数据,将高尿酸作为暴露因素,将房颤作为结局变量。采用MR-Egger法、逆方差加权法(IVW)进行孟德尔随机化分析,研究高尿酸与房颤的因果关系。结果:去除连锁不平衡影响后,最终确定了3个独立且高度相关的SNPs,的r2值<0.01,与高尿酸水平的关联显著(P值小<5×10^(8))。IVW法估计高尿酸与房颤的因果效应,显示两者存在正向的因果联系(OR=1.745,95%CI为1.012-1.355,P=0.033)。结论:高尿酸与房颤之间存在显著的因果关系,对潜在房颤的高尿酸患者实行早期诊断和干预是必要的。展开更多
Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in...Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography （RP-HPLC） with ultraviolet （UV） detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26 （5：95）. Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957-0.9993. The proposed analytical method has satisfactory repeatability （the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49-97.90%, 95.38-96.45%, 112.46-115.78%and 90.82-97.13%with standard deviation of o5%, respectively） and the limits of detection （LODs, S/N Z 3） for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L, respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis＇ biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis.展开更多
目的 探究住院老年2型糖尿病(type 2 diabetes mellitus,T2DM)患者合并肌少症的影响因素。方法 采用病例对照研究,回顾性分析2020年1月至2023年5月在北京医院内分泌科住院的老年T2DM患者,根据亚洲肌少症工作组(Asian Working Group for ...目的 探究住院老年2型糖尿病(type 2 diabetes mellitus,T2DM)患者合并肌少症的影响因素。方法 采用病例对照研究,回顾性分析2020年1月至2023年5月在北京医院内分泌科住院的老年T2DM患者,根据亚洲肌少症工作组(Asian Working Group for Sarcopenia,AWGS)2019年诊断标准诊断肌少症,应用多因素Logistic回归分析探究糖尿病合并肌少症的影响因素。结果 住院老年T2DM患者共469例,男性发生率高于女性(65.3%比34.7%,P<0.05)。糖尿病合并肌少症组(T2DM+Sar组)年龄高于T2DM无肌少症组(T2DM组)(P<0.01)。T2DM+Sar组49例,T2DM组420例。倾向性评分匹配(propensity score matching,PSM)后纳入糖尿病无肌少症组153例(T2DM组),T2DM+Sar组45例,矫正性别、年龄混杂因素后,T2DM+Sar组体质指数(body mass index,BMI)显著低于T2DM组(P <0.01),T2DM+Sar组中,BMI<24kg/m2患者比例更高(53.6%比11.1%,P<0.01),合并脑血管病及糖尿病周围神经病变比例更高(P<0.05)。T2DM+Sar组较T2DM组,Morse跌倒评分更高,日常生活能力(activities of daily living,ADL)评分更低,5次坐站所需时间更长、优势手握力低(均P<0.05),T2DM+Sar组的优势侧上肢臂围更细,总瘦组织含量、骨矿物质含量、内脏脂肪面积均低于T2DM组(P<0.05)。多因素Logistic回归分析显示:合并糖尿病周围神经病变(OR=3.348,95%CI:1.252~8.955)、内脏脂肪面积(OR=1.042,95%CI:1.005~1.079)、血尿酸(OR=1.007,95%CI:1.002~1.012)是老年T2DM合并肌少症的危险因素,BMI (OR=0.512,95%CI:0.363~0.721)、骨矿物质含量(OR=0.037,95%CI:0.008~0.186)是老年T2DM合并肌少症的保护因素。在BMI<24kg/m2的老年T2DM患者群体中,血尿酸(OR=1.010,95%CI:1.003~1.017)为老年T2DM合并肌少症的危险因素,下肢平均腿围(OR=0.624,95%CI:0.447~0.870)、高密度脂蛋白胆固醇(OR=0.195,95%CI:0.040~0.945)、总瘦组织含量(OR=0.889,95%CI:0.797~0.990)为老年T2DM合并肌少症的保护因素。结论 在老年T2DM的管理中,不建议将BMI控制在过低水平,身体成分对于老年T2DM患者合并肌少症的预测作用可能比BMI更有意义。展开更多
文摘Objective: To explore the clinical significance of serum uric acid combined with cystatin C detection in high risk degree of hypertension of different grades. Methods: The patients who were treated in the Department of Cardiovascular Medicine of our hospital from January to December 2022 were selected as the study subjects, and the high risk and extremely high risk groups of grade I, II and III hypertension were selected for comparative analysis to explore their clinical significance. Results: There was a statistically significant difference in the number of patients with extremely high risk of grade II hypertension between men and women (P 0.05), which showed that there was no specificity in the concentration detection of serum uric acid and cystatin C in different grades of hypertension;there was no significant difference in serum uric acid concentration between grade II and grade III of hypertension with high risk grade and cystatin C concentration between grade I and grade III of hypertension with high risk grade (P > 0.05), but there was significant difference in the concentration detection of serum uric acid and cystatin C between the other groups (P < 0.05), which indicates that the detection of serum uric acid and cystatin C has important clinical significance in the high risk degree of hypertension. Conclusion: In the comparison of the detection of blood uric acid and cystatin C in different levels of hypertension with extremely high risk, the difference of cystatin C in grade I and grade III was not statistically significant, and the rest were statistically significant;therefore, the detection of serum uric acid and cystatin C can provide reliable laboratory data for clinical diagnosis and treatment of the extremely high risk degree of different levels of hypertension.
文摘The authors conducted a retrospective study over a period of 6 months in a hypertensive population in order to determine the correlation between serum uric acid on glomerular filtration rate (GFR) and estimated serum potassium with left ventricular hypertrophy (LVH) and obesity. This study enrolled 122 patients including 63 women (51.6%). The mean age was 55.9 ± 10.6 years (range 30 to 74 years). Obesity weight was found in 38 cases (31.1%) of which 20?were men (33.9%) and 18 women (28.6%). Abdominal obesity was found in 104 cases (85.2%). The average serum uric acid in patients with obesity weight was 63.3 ± 18.9 mg/L vs 62.4 ± 14.2 mg/L for no-obese patients (p?= 0.63). The average serum potassium in obese patients was 4.06 ± 0.42 mEq/L vs 4.02 ± 0.46 mEq/L for no-obese (p?= 0.65). The average GFR was 73.4 ± 21.4 ml/L in obese patients vs 66.6 ± 22.6 ml/min in no-obese (p?= 0.03). The LVH was found in 81 cases (66.4%). The LVH was found in 65 (62.5%) obese patients vs?16 (88%) non-obese patients (OR = 4.8, 95% 1.04?-?22?p?= 0.02). Only abdominal?obesity has been correlated with left ventricular hypertrophy after multivariate analysis. Emphasis must be focused on public health actions for effective and appropriate measures against obesity and hypertension, whose prevalence is increasing in our region.
基金supported by the Hundred Talents Program of the Chinese Academy of Sciences (CAS) in 2007the National Natural Sciences Foundation of China (NSFC No.20775083)
文摘Objective To investigate the effect of long-term and low-level occupational Mn exposure on the level of uric acid （UA） in human urine. Methods In this study, 65 volunteers were recruited, who were working on welding and foundry work in an plant in Gansu province, China. Additionally, 29 control samples were collected from individuals who did not have any history of excessive Mn exposure. An improved high performance liquid chromatography system equipped with a diode-array detector （HPLC-DAD） method was developed to determine the UA level in human urine. A Spectra AA 220 Atomic Absorption Spectrophotometer （AAS） was used to measure the Mn level in the urine. Results The analytical method was validated acceptable accuracy, precision, and recovery significantly lower than that of control samples for concentrations ranging from 3.82-45.84 pg/mL with Overall, the UA levels of Mn exposure samples were （P〈O.O5）. Conclusion The practical method developed here is suitable for both routine monitoring of UA level in human urine and metabolism research. Long-term and low-level occupational Mn exposure may lead to a lower UA level in urine, and UA might be an indicator of the early stage of manganism.
文摘目的探索脊柱退变住院患者血清尿酸/高密度脂蛋白比值与骨密度的相关性。方法共纳入803例脊柱退变的受试者,评估临床因素及实验室检查结果,测量骨密度,按照骨密度结果分为骨质疏松症组及非骨质疏松症组。采用多元Logistic回归分析血清尿酸/高密度脂蛋白比值(uric acid to high-density lipoprotein cholesterol ratio,UHR)与骨质疏松症的相关性。结果与非骨质疏松症组相比,骨质疏松症组的UHR更低(244.93±.102.51 vs 199.97±.91.96,P<0.001),多元Logistics回归分析提示,在校正了骨质疏松症的传统危险因素后,UHR最高的四分位患者发生骨质疏松症的可能性是UHR最低的四分位患者的0.402倍(P=0.018)。骨质疏松症的患病率在UHR四分位呈下降的趋势,骨密度在UHR四分位呈升高的趋势。UHR在骨量正常、骨量减少及骨质疏松症3组呈下降的趋势。结论低UHR是昆山地区脊柱退变住院患者发生骨质疏松症的危险因素。对于UHR较低的脊柱退变患者,应注意筛查骨质疏松症。
文摘目的:采用两样本孟德尔随机化(Mendelian Randomization,MR)方法,探讨高尿酸与房颤之间的因果关系。方法:从IEU Open GWAS数据库中检索单核苷酸多态性(SNP)数量最多的文献数据,将高尿酸作为暴露因素,将房颤作为结局变量。采用MR-Egger法、逆方差加权法(IVW)进行孟德尔随机化分析,研究高尿酸与房颤的因果关系。结果:去除连锁不平衡影响后,最终确定了3个独立且高度相关的SNPs,的r2值<0.01,与高尿酸水平的关联显著(P值小<5×10^(8))。IVW法估计高尿酸与房颤的因果效应,显示两者存在正向的因果联系(OR=1.745,95%CI为1.012-1.355,P=0.033)。结论:高尿酸与房颤之间存在显著的因果关系,对潜在房颤的高尿酸患者实行早期诊断和干预是必要的。
基金supported by the National Natural Science Foundation of China(No.21275088)
文摘Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography （RP-HPLC） with ultraviolet （UV） detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26 （5：95）. Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957-0.9993. The proposed analytical method has satisfactory repeatability （the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49-97.90%, 95.38-96.45%, 112.46-115.78%and 90.82-97.13%with standard deviation of o5%, respectively） and the limits of detection （LODs, S/N Z 3） for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L, respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis＇ biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis.
文摘目的 探究住院老年2型糖尿病(type 2 diabetes mellitus,T2DM)患者合并肌少症的影响因素。方法 采用病例对照研究,回顾性分析2020年1月至2023年5月在北京医院内分泌科住院的老年T2DM患者,根据亚洲肌少症工作组(Asian Working Group for Sarcopenia,AWGS)2019年诊断标准诊断肌少症,应用多因素Logistic回归分析探究糖尿病合并肌少症的影响因素。结果 住院老年T2DM患者共469例,男性发生率高于女性(65.3%比34.7%,P<0.05)。糖尿病合并肌少症组(T2DM+Sar组)年龄高于T2DM无肌少症组(T2DM组)(P<0.01)。T2DM+Sar组49例,T2DM组420例。倾向性评分匹配(propensity score matching,PSM)后纳入糖尿病无肌少症组153例(T2DM组),T2DM+Sar组45例,矫正性别、年龄混杂因素后,T2DM+Sar组体质指数(body mass index,BMI)显著低于T2DM组(P <0.01),T2DM+Sar组中,BMI<24kg/m2患者比例更高(53.6%比11.1%,P<0.01),合并脑血管病及糖尿病周围神经病变比例更高(P<0.05)。T2DM+Sar组较T2DM组,Morse跌倒评分更高,日常生活能力(activities of daily living,ADL)评分更低,5次坐站所需时间更长、优势手握力低(均P<0.05),T2DM+Sar组的优势侧上肢臂围更细,总瘦组织含量、骨矿物质含量、内脏脂肪面积均低于T2DM组(P<0.05)。多因素Logistic回归分析显示:合并糖尿病周围神经病变(OR=3.348,95%CI:1.252~8.955)、内脏脂肪面积(OR=1.042,95%CI:1.005~1.079)、血尿酸(OR=1.007,95%CI:1.002~1.012)是老年T2DM合并肌少症的危险因素,BMI (OR=0.512,95%CI:0.363~0.721)、骨矿物质含量(OR=0.037,95%CI:0.008~0.186)是老年T2DM合并肌少症的保护因素。在BMI<24kg/m2的老年T2DM患者群体中,血尿酸(OR=1.010,95%CI:1.003~1.017)为老年T2DM合并肌少症的危险因素,下肢平均腿围(OR=0.624,95%CI:0.447~0.870)、高密度脂蛋白胆固醇(OR=0.195,95%CI:0.040~0.945)、总瘦组织含量(OR=0.889,95%CI:0.797~0.990)为老年T2DM合并肌少症的保护因素。结论 在老年T2DM的管理中,不建议将BMI控制在过低水平,身体成分对于老年T2DM患者合并肌少症的预测作用可能比BMI更有意义。
